胃癌手术前后活化CD69、HLA-DR的变化与临床意义

目的了解胃癌患者围手术期的免疫功能变化,探讨其临床意义.方法应用流式细胞仪检测42例胃癌患者手术前后外周血中T细胞2种表面抗原CD69、HLA-DR,并与良性病变患者进行对照.结果发现胃癌患者术前CD69细胞百分率低于对照组(P＜0.01),HLA-DR细胞百分率与对照组相近(P＞0.05).术后胃癌患者的CD69、HLA-DR细胞百分率较术前均明显增高(P＜0.01).结论胃癌患者术前免疫功能低下,术后活化T细胞明显增多,表明切除肿瘤有利于提高患者的免疫功能,增强机体的抗癌能力,故应尽量争取根治性切除肿瘤.     

Human peritoneal B-1 cells and the influence of continuous ambulatory peritoneal dialysis on peritoneal and peripheral blood mononuclear cell (PBMC) composition and immunoglobulin levels

In mice, peritoneal B cells are composed of a unique B-1 cell population which can repopulate the intestinal lamina propria with IgA-producing cells, as well as contribute to the majority of serum IgM. In this study, peritoneal lymphocytes from patients starting continuous ambulatory peritoneal dialysis (CAPD) and from women undergoing bilateral tubal ligation (BTL) were analysed for the presence of a B-1 cell population as well as the expression of potential homing receptors. Up to 63% of the peritoneal B cells express surface antigen CD5, and most peritoneal lymphocytes express the mucosal homing receptors, α4β7 and αEβ7. When analysing serial samples collected from patients from the beginning of dialysis to 1 year, no marked changes were observed in serum or salivary immunoglobulin levels, although the peritoneal lymphocyte population was reduced by 50%. These data suggest that the phenotype of human peritoneal B-1 cells is similar to that of mice, but the contributions to the immune system may differ.     

Cellular events associated with peripherally induced rejection of mature neural xenografts placed into neonatal rat brains

Various circumstances have brought about a dispute concerning the immunologically priviledged status of the central nervous system (CNS). Using a transplantation paradigm, we have examined the cellular events associated with an experimentally induced focal assault on the CNS by the immune system. Chunks of embryonic mouse cortex were transplanted into neonatal rat brains and allowed to survive for 4 weeks. The adult rats then received a skin graft of donor origin to induce rejection of the transplanted tissue. Animals were sacrificed at various time points and examined histologically and immunocytochemically. Under these circumstances, the transplant is rejected via a first-set rejection response, and astrocytes of donor origin appear to be the primary target of the host immune system. Expression of class I and class II major histocompatibility antigens is noted to correlate with lymphocytic invasion of the transplant.     

^147Pm诱发体细胞和生殖细胞的UDS效应

本文研究了机体摄入不同放射性活度的荧光涂料激发能源１４７Ｐｍ时，诱发外周免疫器官脾淋巴细胞和生殖细胞精子的ＵＤＳ效应。观察结果表明，当机体摄入１４７Ｐｍ的放射性活度处在３．７×１０１～１．８５×１０２Ｂｑ／ｇ体重时，可使ＵＶ诱发脾淋巴细胞和精子的ＵＤＳ值明显增升，使ＤＮＡ的修复能力增强。可是，随着１４７Ｐｍ摄入放射性活度进一步增大至３．７×１０３Ｂｑ／ｇ体重时，观察到ＵＶ诱发的脾淋巴细胞和精子细胞的ＵＤＳ值都呈明显下降，呈现抑制效应，表明１４７Ｐｍ使脾淋巴细胞和精子的ＤＮＡ修复能力受到损伤。     

Intestinal T lymphocytes in the chicken express an integrin-like antigen

We report the characterization of a molecule recognized on chicken T cells by the murine A19 monoclonal antibody that was generated by immunization with intestinal intraepithelial lymphocytes. Immunofluorescence analysis indicated that both αβ and γδ T cell subpopulations in the intestine express the A19 antigen, but natural killer cells and B cells do not. The A19-marked T cells were preferentially localized in the intestinal epithelium and less frequently in the underlying lamina proprial, T cells appearing in the intestine during embryonic life were A19 negative but acquired the antigen within the first few days after hatching. Although rarely found on cells in non-intestinal tissues at any age, very late expression of the A19 antigen could be induced by concanavalin A stimulation of splenic and circulating T cells. Transforming growth factor β1 enhanced this induction of A19 expression. The A19 molecules expressed by intestinal T cells and activated splenic T cells were biochemically identical, consisting of a multi-molecular complex of proteins with approximate M_r of 205, 145 and 75 kDa under nonreducing conditions and 120, 90 and 28 kDa under reducing conditions. The characteristics of this multimolecular complex and its differential expression suggest that the A19 antigen is a member of the integrin family which may function in the retention of intestinal lymphocytes.     

High grade malignant lymphoma with clinical characteristics and immunophenotype of natural killer cells

The malignant proliferation of natural killer (NK) cells which are morphologically characterized as large granular lymphocytes (LGL) is a well known clinical entity which was named after its morphological appearance as LGL-leukemia/lymphoma. Similar to non-malignant NK-cells, these tumors can be divided into those which express the CD3-T-cell receptor complex and those which do not. The CD3-positive type of LGL-leukemia is immunophenotypologically characterized by the expression of CD16, and variably CD 56 and CD 57, and generally follows a more indolent course. In contrast, malignant proliferations of CD3-negative LGL express either CD16 or CD 56, and only occasionally CD 57 on their cell surface. Clinically, CD3-negative NK-lymphomas tend to progress rapidly. We report here the case of a high grade malignant lymphoma which was characterized by an immunophenotype typical for CD3-negative NK-cells (CD2+, CD3?, CD16+, CD56(+), CD57?). The disease proved to be rapidly fatal despite aggressive chemotherapy. Interestingly, the patient suffered from a high turn over pancytopenia, which also characterizes NK-cell leukemias/lymphomas of the LGL-type. However, our patient's lymphatic cells appeared highly immature, and cytoplasmic granules, characteristic for LGL-cells, could not be discerned either microscopically or electronmicroscopically. Furthermore, the malignant lymphatic population had the T-cell receptor α-chain rearranged. We therefore concluded that our patient might have suffered from a malignant proliferation of a putative precursor cell intermediate between T-cells and NK-cells. © 1995 Wiley-Liss, Inc.     

模拟失重和辐射对大鼠免疫功能和垂体β—内啡肽含量的影响

本文研究的目的的是探讨短期模拟失重、电离辐射及两者复合因素对大鼠免疫的功能的影响及其免疫功能改变的原因。大鼠为实验动物，尾悬吊为模拟失重动物模型＾６０Ｃｏ为辐射源。