八珍汤对血虚模型小鼠造血调控因子影响的实验研究

探讨八珍汤对由环磷酰胺引起的小鼠骨髓造血功能抑制的调控作用。采用环磷酰胺致小鼠血虚模型 ,测定八珍汤对骨髓抑制小鼠外周血象及其细胞因子产生的影响。结果表明 ,八珍汤对环磷酰胺所致血虚模型小鼠骨髓细胞有促进增殖作用 ;经八珍汤诱导制备的巨噬细胞、脾细胞、肺条件培养液和骨骼肌条件培养液能促进血虚模型小鼠骨髓细胞增殖 ,促进血虚模型小鼠骨髓基质细胞分泌肿瘤坏死因子 (TNF)。八珍汤对环磷酰胺所致化疗损伤的造血调控作用可能与直接或间接刺激造血微环境的基质细胞分泌正性和负性造血生长因子有关。     

The N-terminal flanking region of the invariant chain peptide augments the immunogenicity of a cryptic "self" epitope from a tumor-associated antigen

The N-terminal flanking region of the invariant chain peptide termed CLIP appears to have superagonistic properties interacting with the T cell receptor and the MHC class II molecule at or near the binding site for the bacterial superantigen Staphylococcal enterotoxin B (SEB). The present studies explored the hypothesis that the N-terminal segment of CLIP can augment the immunogenicity of cryptic "self" tumor-associated antigens. A chimeric construct of an MHC class II binding peptide from the c-erb oncogene (Her-2/neu) containing the N-terminal flanking region of CLIP elicited potent antitumor activity against a Her-2/neu-positive tumor in a rat model system. Comparatively, the unmodified parent peptide was ineffective. The induction of effective antitumor immunity, however, required presentation of the chimeric peptide construct on irradiated tumor cells or the peptide construct in concert with a Her-2/neu MHC class I-restricted peptide from Her-2/neu. As revealed by adoptive transfer studies, effective protective antitumor immunity in this setting required the CD4 T helper subset. Additionally, in vitro analysis revealed that immunization with the parent peptide resulted in a weak immune response to the unmodified peptide consisting of both type 1 (IL-2, IFN-gamma) and type 2 (IL-4, IL-10) cytokine-producing cells analyzed by RT-PCR (qualitative and quantitative) and by limiting dilution assay. Comparatively, immunization with the chimeric construct elicited a potent immune response to the parent peptide with predominantly type 1 cytokine-producing cells. Taken together, the results suggest that immunization with the chimeric Her-2/neu peptide induced protective antitumor immunity. Associated with this immunization strategy was the enhancement of a type 1 cytokine response.     

Hydrophobically Modified Poly(acrylic acid) Using 3‐Pentadecylcyclohexylamine: Synthesis and Rheology

Summary: Hydrophobically modified poly(acrylic acid) was synthesized using 3‐pentadecylcyclohexylamine (3‐PDCA), which was in turn synthesized from 3‐pentadecylphenol, one of the components of cashew‐nut shell liquid (CNSL), a renewable resource material. ¹H NMR spectra confirmed the incorporation of 3‐PDCA onto PAA and a series of HMPs with three different molar concentrations, viz ? 3, 5 and 7 mol‐% of 3‐PDCA, were synthesized. An increase in viscosity with increasing hydrophobic content was observed by rheological measurements. The critical association concentrations were determined using an Ubbelohde viscometer and a controlled stress rheometer. The stability of HMPs towards temperature and shear was studied. Rheological measurements showed that there was a steady increase in viscosity with increase in hydrophobe content due to the formation of reversible networks. These polymers exhibited gel‐like behavior at low concentrations (≥2 wt.‐%) with an apparent yield stress (ca. 10 Pa) and showed shear thinning properties (non‐Newtonian). However, below a critical concentration, c [η], they showed Newtonian behavior.

η_sp of unmodified and modified PAA‐Na at various polymer concentrations.     

从氧自由基、一氧化氮探讨四逆汤抗急性失血性休克的肝脏机制

目的:从氧自由基、一氧化氮探讨四逆汤抗急性失血性休克的肝脏机制。方法:复制急性失血性休克大鼠模型, 分为假手术对照组;单纯休克模型组;休克+生理盐水复苏组;休克+四逆注射液复苏组。四逆注射液(浓度1000g生药/L), 剂量0.1mL/200g大鼠。用生理盐水或四逆注射液治疗3h后处死动物并取组织。测定各组肝脏超氧化物歧化酶(SOD)活性、丙二醛(MDA)水平, 一氧化氮(NO)水平。采用免疫组化染色法, 观察诱导型一氧化氮合酶(iNOS)在肝细胞中的变化特点。RT-PCR观测肝细胞iNOS和内皮源性一氧化氮合酶(eNOS)基因表达的变化。结果:模型组在休克1h后SOD活性明显低于对照组(P＜0.01)、MDA水平明显高于对照组(P＜0.01)。四逆汤组复苏3h后肝组织SOD明显高于生理盐水组(P＜0.01)、MDA低于生理盐水组(P＜0.01)、NO水平明显高于生理盐水组(P＜0.01)。生理盐水组iNOS在肝细胞中染色阳性单位明显高于四逆汤组(P＜0.05)。生理盐水组促进iNOSmRNA的表达。四逆汤组eNOSmRNA的表达增强。结论:四逆汤通过清除氧自由基, 升高NO, 改善肝组织微循环, 减少诱导型iNOS表达的各种因素, 理论上减轻了NO与氧自由基生成的ONOO的细胞毒作用和血管的低反应性, 并对肝脏起到保护作用。     

Association between HLA and Japanese patients with rheumatoid arthritis

Japanese patients with rheumatoid arthritis (RA) were observed to have a statistical association with HLA-DR4, MT3. Strong association between the clinical severity of RA and HLA was also observed. Male patients had a stronger association with HLA than female patients. Males are more resistant to RA than females. This suggested that the threshold of liability for RA is higher in males than in females. Japanese patients with RA with systemic vasculitis were negative for HLA-Bw44 and had antilymphocytotoxic autoantibody, indicating that RA with systemic vasculitis is different in etiology from RA without systemic vasculitis.     

羌活胜湿汤加减联合臭氧注射、射频热凝靶点穿刺术治疗神经根型颈椎病风寒痹阻型临床观察

目的 观察羌活胜湿汤加减联合臭氧注射、射频热凝靶点穿刺术治疗神经根型颈椎病（风寒痹阻型）的临床疗效。方法 选择2020年8月至2021年5月于河南省中医院治疗的68例神经根型颈椎病患者,以随机数字表法分组,34例患者为观察组,患者为对照组,对照组给予羌活胜湿汤安慰剂颗粒,同时予臭氧注射、射频热凝靶点穿刺术治疗,观察组给予羌活胜湿汤加减联合臭氧注射、射频热凝靶点穿刺术治疗,治疗前后检测两组患者肿瘤坏死因子-α（TNF-α）、降钙素原（PCT）、白细胞介素-2（IL-2）、白细胞介素-1β（IL-1β）、6-酮前列腺素E1α（6-keto-PGE1α）、血浆P物质（SP）、β内啡肽（β-EP）、脂质过氧化物（LPO）、丙二醛（MDA）、超氧化物歧化酶（SOD）水平,给予患者疼痛视觉量表（VAS）、颈椎功能障碍指数量表（NDI）、颈椎病临床评价量表（CASCS）评价,观察临床疗效。结果 与本组治疗前比较,治疗后两组患者IL-1β、PCT、TNF-α水平降低,IL-2水平升高（P<0.05）;治疗后与对照组比较,观察组IL-1β、PCT、TNF-α、IL-2改善更明显（P<0.05）。与本组治疗前比较,治疗后两组患者SOD水平升高,LPO、MDA水平降低（P<0.05）;治疗后与对照组比较,观察组SOD、LPO、MDA改善更明显（P<0.05）。与本组治疗前比较,治疗后两组患者SP、6-keto-PGE1α水平降低,β-EP水平升高（P<0.05）;治疗后与对照组比较,观察组SP、6-keto-PGE1α、β-EP改善更明显（P<0.05）。与本组治疗前比较,治疗后观察组VAS、NDI评分降低,CASCS评分升高（P<0.05）;治疗后与对照组比较,观察组VAS、NDI、CASCS评分改善更明显（P<0.05）。治疗后,观察组患者总有效率高于对照组（Z=2.018,P<0.05）。结论 羌活胜湿汤加减联合臭氧注射、射频热凝靶点穿刺术治疗神经根型颈椎病（风寒痹阻型）患者,可抑制患者炎症,清除氧自由基,改善疼痛介质水平,缓解患者疼痛,提升颈椎功能及临床疗效。     

电子鼻技术应用于白及及其近似饮片快速辨识的可行性分析

目的：探讨电子鼻技术应用于白及及其近似饮片快速辨识的可行性。方法：收集134批白及及其近似饮片（白及45批、天麻30批、玉竹30批、黄花白及29批）作为待测样品,使用PEN3型电子鼻采集样品嗅觉感官数据作为自变量X,基于2020年版《中华人民共和国药典》和地方标准的鉴别结果,以及各饮片高效液相色谱法（HPLC）指纹图谱和原始采购信息,获得辨识模型的标杆数据Y,分别采用主成分分析-判别分析（PCA-DA）、偏最小二乘法-判别分析（PLS-DA）、最小二乘法-支持向量机（LS-SVM）及K-最近邻（KNN）4种化学计量学方法建立45批白及与89批非白及的二分类辨识模型和上述4种饮片的四分类辨识模型Y=F(X)。结果：经留一法交互验证,在二分类辨识中,上述4种模型分类正判率分别为97.01%、97.01%、98.51%和97.01%;在四分类辨识中,这4种模型分类正判率分别为97.76%、89.55%、98.51%和97.01%。二分类和四分类辨识模型的最高正判率均可达到98.51%,且均以LS-SVM算法为最优,最优核函数分别选择径向基核函数和线性核函数。最优模型判别结果良好,没有未分类样...     

黄芪桂枝五物汤加减对糖尿病大鼠坐骨神经细胞凋亡相关Bax和Caspase-12的影响

目的 研究黄芪桂枝五物汤加减对糖尿病大鼠坐骨神经细胞凋亡相关B细胞淋巴瘤-2相关X蛋白（Bax）和胱天蛋白酶-12（Caspase-12）蛋白与mRNA表达的影响,以探究黄芪桂枝五物汤加减治疗糖尿病周围神经病变的作用机制。方法 选用动物实验方法进行研究,将60只雄性SD大鼠通过高糖高脂饲料喂养联合链尿佐菌素（STZ）腹腔注射诱导成糖尿病大鼠动物模型,连续3 d随机血糖≥16.7 mmol·L^-1者为糖尿病大鼠造模成功,将48只造模成功的糖尿病大鼠随机分为模型组、α-硫辛酸组（0.026 8 g·kg^-1·d^-1）、中药高、低剂量组（2.5、1.25 g·kg^-1·d^-1）,每组各12只,并设正常组10只。监测大鼠体质量和随机血糖水平;干预16周末通过Key point肌电采集系统检测大鼠坐骨神经传导速度;分别通过蛋白免疫印迹法（Western blot）和实时荧光定量聚合酶链式反应（Real-time PCR）检测大鼠坐骨神经中Bax和Caspase-12蛋白与mRNA的表达。结果 与正常组比较,模型组大鼠体质量显著下降（P<0.01）,随机血糖水平显著升高（P<0.01）;干预16周,与模型组比较,中药高剂量组大鼠体质量明显升高（P<0.05）,其他给药组体质量变化差异无统计学意义;各给药组随机血糖水平均显著降低（P<0.01）。与正常组比较,干预16周,模型组大鼠运动和感觉神经传导速度显著降低（P<0.01）;与模型组比较,各给药组大鼠运动和感觉神经传导速度均明显升高（P<0.05,P<0.01）。与正常组比较,模型组大鼠坐骨神经Bax和Caspase-12蛋白表达显著升高（P<0.01）;与模型组比较,各给药组大鼠坐骨神经Bax和Caspase-12蛋白表达均显著降低（P<0.01）。与正常组比较,模型组大鼠坐骨神经Bax和Caspase-12 mRNA表达显著升高（P<0.01）;与模型组比较,α-硫辛酸组、中药高剂量组大鼠坐骨神经Bax mRNA表达明显降低（P<0.05,P<0.01）,中药低剂量组坐骨神经Bax mRNA表达降低有下降趋势;各给药组大鼠坐骨神经Caspase-12 mRNA表达显著降低（P<0.01）。结论 黄芪桂枝五物汤加减可能通过抑制坐骨神经细胞凋亡来改善和修复糖尿病大鼠坐骨神经损伤。     

加味瓜蒌枳实汤联合哌拉西林钠他唑巴坦钠对痰热蕴肺型卒中相关性肺炎患者的免疫功能和血清炎症因子水平的影响

目的：探究加味瓜蒌枳实汤联合哌拉西林钠他唑巴坦钠对痰热蕴肺型卒中相关性肺炎(SAP)患者的免疫功能和血清炎症因子水平的影响。方法：将80例痰热蕴肺型SAP患者随机分为对照组与观察组,各40例,对照组SAP患者在基础治疗上加用哌拉西林钠他唑巴坦钠,观察组患者在对照组治疗基础上加用加味瓜蒌枳实汤,连续治疗2周,并比较两组SAP患者治疗前后的临床治疗效果、免疫指标、炎症指标及肺功能情况。结果：与本组治疗前比较,两组SAP患者治疗后X射线片病灶、肺啰音、咳嗽、发热、痰色、便秘评分均明显降低(P<0.05);两组SAP患者的第1秒内用力呼气量占呼气量的比例(FEV1/FVC)、第1秒用力呼气容积百分比(FEV1%)均明显改善(P<0.05)。与对照组治疗后比较,观察组患者的FEV1/FVC、FEV1%改善更明显(P<0.05)。与本组治疗前比较,两组SAP患者经治疗后中性粒细胞与淋巴细胞比值(NLR值)均明显降低(P<0.05);与对照组治疗后比较,观察组患者的NLR值明显降低(P<0.05)。与本组治疗前比较,两组SAP患者在治疗后的血清降钙素原(PCT)、超敏C...