携带HPV11型基因组细胞三维培养模型的建立及其衣壳蛋白L1的表达

目的用携带人乳头瘤病毒11型(HPV11)基因组的角质形成细胞(HPV11.HaCaT细胞),在体外建立皮肤类似培养物三维模型,观察HPV11衣壳蛋白L1是否表达,为进一步建立体外病毒复制系统和药效筛选模型奠定基础。方法用人成纤维细胞和I型鼠尾胶原制备真皮类似物凝胶,接种HPV11.HaCaT细胞进行三维培养。同时以正常HaCaT细胞作对照。15天时取皮肤类似物进行冰冻切片,HE染色进行组织结构检查,免疫组化法检查角蛋白10、泛角蛋白以及HPV11L1蛋白表达。结果HE染色显示,HPV11.HaCaT细胞和正常HaCaT细胞的层数明显增多。免疫组化显示,两种细胞的皮肤类似物角蛋白10、泛角蛋白表达全部阳性。HPV11.HaCaT细胞表达L1蛋白阳性,而正常HaCaT表达L1蛋白阴性。结论三维培养方式可诱导HPV11.HaCaT细胞分化,皮肤类似培养物出现细胞分化标志,并且表达HPV11型衣壳蛋白L1。     

α-干扰素栓联合制霉菌素治疗外阴阴道念珠菌病疗效观察

目的：探讨α-干扰素栓联合制霉菌素治疗外阴阴道念珠菌病疗效。方法：随机抽取240例外阴阴道念珠菌病患者。随机分为3组：α-干扰素栓组（A组）、霉菌素组（B组）、α-干扰素栓联合霉菌素组（C组）,每组各80例。所有患者于治疗后第2,4、8周随访,治疗后4周判定疗效,并比较3组治疗结束后疗效和复发率。结果：停药后4周,A、B、C三组的总有效率分别为82．5％、85．0％和95．0％。A、B组与C组比较,差异有统计学意义（P〈0．05）。停药后8周复发率：对照A组为40．0％,对照B组为37．5％,C组为12．5％,A、B组与C组比较,差异有统计学意义（P〈0．05）。结论：α-干扰素栓联合制霉菌素治疗外阴阴道念珠菌病具有高效、复发率低、起效快、安全等特点,值得临床推广。     

NP方案联合三维适形放疗治疗局部晚期非小细胞肺癌的疗效及安全性观察

目的：观察NP方案联合三维适形放疗治疗局部晚期非小细胞肺癌的疗效及安全性。方法：56例局部晚期非小细胞肺癌患者根据治疗方法不同随机分为三维适形放疗联合NP方案化疗组（观察组,28例）与单纯放疗组（对照组,28例）,比较两组疗效及毒副反应情况。结果：观察组的疗效明显高于对照组（P〈0.05）。两组的1年生存率、2年生存率比较,P〈0.05,差异有统计学意义。结论：NP方案联合三维适形放疗治疗局部晚期非小细胞肺癌的疗效确切,有效率高,近期疗效理想,提高了患者的生存率。     

不同产地急性子中人体必需微量元素含量测定与分析

目的:分析不同产地急性子中9种人体必需微量元素的含量,评价药材质量。方法:采用ICP-MS法测定急性子微量元素含量,使用SPSS软件分析急性子中微量元素之间的相关性,并通过主成分分析对药材进行质量评价。结果:Fe,Zn,Mn,Cu等对人体有益元素含量丰富。微量元素含量间具有显著相关性。主成分分析确定综合评价函数,其函数为F=0.209x1+0.256x2+1.194x3+0.236x4+0.267x5+0.044x6+0.196x7-0.084x8+0.263x9。结论:9种人体必需微量元素对急性子的药效起主要或辅助的作用,不同产地间含量差异较大,可为不同产地急性子药材质量评价提供科学依据。     

基于知识元的中医药信息知识标引

目的建立中医药知识标引库。方法基于文献的题目、摘要以及关键词,采用文献标引方法、Delphi法和计算机软件采集知识标引。结果确立了知识元描述原则,从文献中提取具有知识元属性的特征词,从而构建中医药信息知识标引库。结论与文献内容密切相关的知识元标引研究,是实现知识管理系统的构建研究的关键点。     

从证素分布特点探讨艾滋病病因

目的：研究艾滋病（AIDS）中医证素分布特点,探讨艾滋病的致病因素。方法：采用流行病学调查设计和现场问卷调查方法,选择艾滋病高发的河南省、新疆维吾尔族自治区、云南省和广东省各市、县、乡、村,随机抽样AIDS患者790例,并收集同地区非HIV/AIDS 390例作对照组,运用＂WF文锋-Ⅲ中医辅助诊疗系统＂软件诊断并统计分析。结果：两组调查对象基本情况基线一致,病位和病性证素对比都有统计学意义。结论：艾滋病患者在病性证素上表现为气虚、血虚、阴虚、阳虚和湿、痰、热等,在病位证素上以肾、肺、脾、肝等脏为主。     

髋关节发育不良髋臼内壁内移截骨全髋置换术的三维有限元分析

目的利用三维有限元力学分析方法,研究髋关节发育不良患者行髋臼内壁内移截骨全髋关节置换术对髋臼假体-骨界面间的应力分布情况的影响。方法选取髋关节发育不良患者的骨盆为实验对象,用螺旋CT做全长连续扫描,然后利用计算机仿真技术对CT图像进行三维重建,建立髋关节发育不良骨盆模型。利用计算机辅助设计(CAD)功能,在计算机环境中对重建模型进行髋臼内壁内移截骨全髋关节置换的模拟手术,利用有限元分析软件SolidWorks对重建模型进行有限元网格化,最后使用SolidWorks软件中的力学分析工具包COSMOS对网格化的模型进行力学分析,得到髋臼假体-骨界面间的应力分布情况的实验结果,对实验结果进行统计和分析,观察术后髋臼假体-骨界面间的应力分布情况。结果术后髋臼压应力分布符合后上象限最大,前下象限最小,前上象限、后下象限居中的趋势。剪切应力分布情况为后上象限剪切应力最大,其他象限无差异。结论髋臼内壁内移截骨术没有改变正常髋臼的应力分布情况。     

民族药铁棒锤不同炮制品中各元素的光谱分析

目的:探讨民族药铁棒锤不同炮制品中各元素的种类及含量。方法:利用电感耦合等离子体发射光谱法(ICP-AES)、原子吸收光谱法(AAS)和原子荧光光谱法(AFS)全面扫描并分析民族药铁棒锤生品及其8种不同炮制品中与人体健康和生命相关的常量元素、微量元素和重金属的种类及含量。结果:从铁棒锤中共检测出22种元素,与生品相比,不同炮制方法使某些元素发生了明显不同的变化,表明元素变化与炮制方法有较强的相关性。结论:3种光谱分析方法均能快速准确地分析药材中各种元素含量及其变化趋势,说明炮制和辅料不仅能改变元素含量而且能与有效成分一道发挥协同治疗疾病的效果,并发现藏医和羌医的炮制方法将导致重金属Hg的含量急剧增高,临床使用铁棒锤时存在安全隐患。实验拓宽了光谱法在中药安全性评价中的应用。     

Suppression of WIF-1 through promoter hypermethylation causes accelerated proliferation of the aryl hydrocarbon receptor (AHR) overexpressing MCF10AT1 breast cancer cells

The cause for increased cell proliferation in AHR overexpressing breast cancer cells still remains unknown. Here we studied the molecular basis of aggressive cell proliferation of an AHR overexpressing and ERα functionally down-regulated MCF10AT1 cell line, designated as P20E, in comparison to a matched sub-line, P20C with normal AHR expression and ERα function. We found that a 4-day treatment of P20E cells with 5-aza-2′-deoxycytidine (AZ) caused a significant suppression of cell proliferation. Such an effect of AZ was accompanied with the significant recovery of ERα function. Among diagnostic markers of AZ-induced cellular changes we found conspicuous up-regulation of mRNA expression of Wnt inhibitory factor-1 (WIF-1), particularly in P20E. The possibility of AZ-induced demethylation on the promoter of WIF-1 gene was confirmed through methylation specific PCR assay. Such AZ-induced changes in P20E cells were also accompanied with the decrease in the binding of nuclear proteins to the ³²P labeled TRE (TCF response element) and the reduced accumulation of β-catenin protein in the cell nucleus, indicating the importance of Wnt/β-catenin pathway in maintaining the increased cell proliferation in P20E line over P20C line. The importance of WIF-1 in this regard has been validated by transfecting cells with siRNA against WIF-1, which caused an increase in cell proliferation. Moreover, artificial overexpression of ERα in both P20E as well as MDA-MB-231 cells increased the mRNA expression of WIF-1. Together these results support our main conclusion that the aggressive cell proliferation of this AHR-overexpressing sub-type of transformed mammary epithelial cells is assisted by the epigenetic suppression of WIF-1 gene, which is caused by the loss of ERα.     

Experiments and numerical modeling to estimate the coating variability in a pan coater

The purpose of this work is to investigate the effect of the coating process parameters on coating performance and coating variability, and hence determine the optimal operating conditions. Coating of particles is done to mask the unpleasant taste or odor of the drug, to control the bioavailability of the API, and to increase shelf-life. The coating solution is sprayed in specific locations of the granular bed and coating uniformity is achieved by interparticle collisions and overall mixing behavior in the coater. Thus, good understanding of particle flow and granular mixing in a pan coater is vital to optimize the process parameters to reduce coating variability. Coating experiments are performed at previously determined optimal mixing conditions using Lactose nonpareils. The coating fluid (aqueous solution of Opadry II) is sprayed intermittently at different flow rates and concentration. Vernier Caliper is used to measure the change in diameter and the coating of the particles. Moreover, DEM based numerical modeling of spray coating is also performed for same operational parameter set and spray characteristic (center and the radius of the spray zone) used in the experiments. DEM simulation provides the residence time distribution of all the particles passing through the spray zone. The coating variability in the experiments is estimated at different pan and spray variables. The coating variability decreases with the increase inpan tilt, coating time and an optimum speed. The spray characteristics does not seem to have much effect on the variability although better coating is observed under better mixing conditions of high tilt and pan speed for the same spray parameters. The mass distribution of coated particles is quantified in the numerical model by the total number of particles passing through the spray zone and also by the frequency distribution of the residence time of the coated particles. It is observed that the simulations are in good agreement with the experiments for the effect of orientation (tilt) of the pan coater on coating variability. However simulations over predicted the effect of speed as compared to the experiments to reach the minimum coating variability. In the current study, the experimental setup did not reflect the typical bead coating setup used in the industry; rather depict a simplified setup to validate the numerical model.