人p66Shc重组腺病毒抑制Hela细胞增殖的体外研究

目的 探讨人p66Shc重组腺病毒抑制人宫颈癌Hela细胞增殖的作用及机制.方法 Hela细胞分为空白对照组(不感染腺病毒)、阴性对照组(感染Adeno X-LacZ)和实验组(感染AdenoX-p66Shc).通过细胞生长曲线、MTT检测分析观察细胞的生长状态;DHE染色法检测细胞内活性氧生成;流式细胞仪检测细胞周期;Western Blot法检测细胞内相关蛋白的表达.结果 p66Shc重组腺病毒能够抑制Hela细胞增殖,且随着p66Shc重组腺病毒处理时间延长和剂量增加其抑制作用增强.与空白和阴性对照组比较,p66Shc重组腺病毒感染48 h后,G2/M期细胞比例明显升高,其差异具有统计学意义(P＜0.01);同时引起Hela细胞活性氧水平显著上升,p53、CyclinB1蛋白表达显著升高,其差异具有统计学意义(P＜0.05).结论 p66Shc重组腺病毒能够显著抑制Hela细胞增殖,并诱导其发生G2/M期阻滞.     

Shear bond strength of an autopolymerizing repair resin to injection-molded thermoplastic denture base resins

Abstract

Objective. This study investigated the shear bond strength of an autopolymerizing repair resin to injection-molded thermoplastic denture base resins. Materials and methods. Four injection-molded thermoplastic resins (two polyamides, a polyethylene terephthalate copolymer and a polycarbonate) were used in this study. The specimens were divided into eight groups according to the type of surface treatment given: (1) no treatment, (2) air abrasion with alumina, (3) dichloromethane, (4) ethyl acetate, (5) 4-META/MMA-TBB resin, (6) alumina and 4-META/MMA-TBB resin, (7) tribochemical silica coating or (8) tribochemical silica coating and 4-META/MMA-TBB resin. Half of the specimens in groups 1, 5, 6 and 8 were thermocycled for 10,000 cycles in water between 5–55°C with a dwell time of 1 min at each temperature. The shear bond strengths were determined. Results. The shear bond strengths to the two polyamides treated with alumina, dichloromethane and ethyl acetate and no treatment were very low. The greatest post-thermocycling bond strengths to polyamides were recorded for the specimens treated with tribochemical silica coating and 4-META/MMA-TBB resin (PA12: 16.4 MPa, PACM12: 17.5 MPa). The greatest post-thermocycling bond strengths to polyethylene terephthalate copolymer and polycarbonate were recorded for the treatment with alumina and 4-META/MMA-TBB resin (22.7 MPa, 20.8 MPa). Conclusion. Polyamide was exceedingly difficult to bond to an autopolymerizing repair resin; the shear bond strength improved using tribochemical silica coating followed by the application of 4-META/MMA-TBB resin. Both polyethylene terephthalate copolymer and polycarbonate were originally easy to bond to an autopolymerizing repair resin. However, with 4-META/MMA-TBB resin, the bond was more secure.     

脂肪干细胞复合载淫羊藿苷支架材料对兔下颌骨缺损的修复

目的:研究脂肪干细胞(ADSCs)作为种子细胞复合淫羊藿苷支架材料修复兔下颌骨缺损的效果.方法:体外培养ADSCs,茜素红染色检测骨向分化.实验组在骨缺损处植入ADSCs复合载淫羊藿苷支架材料;对照组2植入单纯纳米羟基磷灰石(n-HA)材料;对照组2植入淫羊藿苷/n-HA复合材料;空白组不植入任何材料.应用X线、组织学、扫描电镜观察并比较植入物与骨组织交界处的成骨修复情况.结果:实验组骨缺损区完全被骨组织修复,边界已融合;对照组1部分修复,有少量炎性渗出;对照组2大部分已修复,边界模糊;空白组基本未见修复.结论:ADSCs复合载淫羊藿苷支架材料具有骨缺损修复能力,其效果优于淫羊藿苷/n-HA材料及单纯n-HA材料.     

n—HA／PA66Cage在腰椎融合术中的生物安全性研究

目的评价新型纳米羟基磷灰石／聚酰胺66融合器（nano-hydroxyapatite／polyamide66Cage,n—HA／PA66Cage）植人人体内可能引起的全身毒性反应及对人体局部组织的影响。方法2012年2月至2012年4月将n—FLA／PA66Cage通过腰椎后路经椎间孔进行腰椎椎体融合植入20例患者体内,通过对研究对象检查术前、术后4d、术后2个月等3个时期的血压、脉搏、体温、免疫球蛋白A、G、M、补体C3、C4、谷丙转氨酶、谷草转氨酶、肌酐、尿素、白细胞、红细胞、血红蛋白、血小板、C反应蛋白、血沉、局部反应等指标。结果n—HA／PA66Cage植人人体后,除了术后4d白细胞、红细胞、血红蛋白、血小板等血常规、C反应蛋白、血沉等检查与术前相比,存在统计学差异（P〈0．05）;在不同时相,其他检查结果之间无统计学意义（P〉0．05）。结论新型n—HA／PA66Cage具有良好的生物安全性。     

Comparison of anterior cervical fusion by titanium mesh cage versus nano-hydroxyapatite/polyamide cage following single-level corpectomy

Purpose

The titanium mesh cage (TMC) is a typical metal cage device which has been widely used in cervical reconstruction for decades. Nano-hydroxyapatite/polyamide-66 (n-HA/PA66) cage is a novel biomimetic non-metal cage device growing in popularity in many medical centres in recent years. There has been no comparison of the efficacy between these two anterior reconstructing cages. The purpose of this study was to compare the radiographic and clinical outcomes of these two different devices.

Methods

Sixty-seven eligible patients with single-level ACCF using TMC or n-HA/PA66 cage for cervical degenerative diseases, with four-year minimum follow-up, were included in this prospective non-randomised comparative study. Their radiographic (cage subsidence, fusion status, segmental sagittal alignment [SSA]) and clinical (VAS and JOA scales) data before surgery and at each follow-up was recorded completely.

Results

The fusion rate of the n-HA/PA66 group was higher than TMC at one year after surgery (94 % vs. 84 %) though their finial fusion rates were similar (97 % vs. 94 %). Finial n-HA/PA66 cage subsidence was 1.5 mm with 6 % of severe subsidence over three millimetres, which was significantly lower than the respective 2.9 mm and 22 % of TMC (P < 0.0001). Lastly, SSA, VAS and JOA in TMC group were worse than in the n-HA/PA66 group (P = 0.235, 0.034 and 0.007, respectively).

Conclusions

The n-HA/PA66 cage is associated with earlier radiographic fusion, less subsidence and better clinical results than TMC within four years after one-level ACCF. With the added benefit of radiolucency, the n-HA/PA66 cage may be superior to TMC in anterior cervical construction.     

The lack of association between catechol-O-methyltransferase (COMT) Val108/158Met and brain-derived neurotrophic factor (BDNF) Val66Met polymorphisms and schizophrenia in a group of Turkish population

IntroductionSchizophrenia is a complex neuropsychiatric disorder with deficits of multiple domains of cognitive functions, volition and emotions. Family and twin studies have provided cumulative evidence for the genetic basis of schizophrenia. The aetiolgy of this disease involves the interplay of multifactiorial inheritance operating on brain maturational processes and polygenic inheritance with some genes showing susceptibility at many genomic locations such as 22q and 11q.The catechol-O-methyltransferase (COMT-22q11) is an extensively studied candidate gene for schizophrenia. COMT acts as an enzymatic detoxicating barrier between the blood and other tissues regulating the amounts of active dopamine and norepinephrine in various parts of the brain and therefore to be associated with schizophrenia.The presence of a common functional single nucleotide polymorphism (SNP) in exon 4 [Guanine (G) Adenine (A); Val108/158Met], alters the enzymatic activity with a trimodal distribution of high-HH, intermediate-HL and low-LL activity alleles which appear to have association with schizophrenia.Brain-derived neurotrophic factor (BDNF-11q13) is a member of the nerve growth factor family working as a molecular regulator of neuronal development and plasticity. Molecules that are critical in the development and survival of neurons such as BDNF play a significant role in the neuropathology of schizophrenia. While upregulation of BDNF increases the neuronal cell size and synaptic plasticity, a functional polymorphism at codon 66 [G→A; Val66Met] down regulates this process and induces schizophrenia.ObjectiveIn the present study, our aim was to investigate the differences in allele frequencies between schizophrenic patients [n = 97 (51 men, 46 women)] and control group [n = 376 (228 men, 148 women)] subjects.ResultsWhen the control and schizophrenia groups were compared for BDNFVal66Met polymorphism, we did not find a significant difference between the study groups either for genotype (χ² = 3.370447, p > 0.05) or Val/Met haplotype analysis (χ² = 2.840264, p > 0.05). When a comparison was revealed for COMT-Val108/158Met polymorphism, no significant difference was detected among schizophrenia and control groups for genotype (χ² = 0.373330, p > 0.05) and Val/Met haplotype analysis (χ² = 0.339073, p > 0.05). When the control and study groups were compared for BDNFVal66Met–COMTVal108/158Met polymorphisms compound genotype and haplotype analyses, there was no significant difference between the two groups (χ² = 11.015; p > 0.05 and χ² = 3.191; p > 0.05), respectively.ConclusionOur results indicate that there is no association between schizophrenia and BDNF–COMT polymorphisms and haplotypes analysis. We also did not find an association between schizophrenia and BDNF–COMT compound genotype and haplotype analyses. Although our study is unique in Turkey as combining BDNF and COMT compound genotype–haplotype analyses, for a generalization of Turkish schizophrenia patient's susceptibility to schizophrenia; we need further studies with an enlarged cohort.     

弥漫性轴索损伤BDNF及其Val66Met基因多态性与认知功能的相关性研究

目的探讨弥漫性轴索损伤（DAI）（Ⅱ型）患者伤后1个月血清脑源性神经营养因子（BDNF）水平及其Val66Met基因多态性与认知功能的关系。 方法选取晋江市医院神经外科自2015年8月至2020年8月收治的106例DAI（Ⅱ型）患者为病例组,选择同期来本院体检的105名健康体检者为对照组,采用第二版洛文斯顿作业疗法认知量表（LOTCA）、蒙特利尔评估量表中文版（MoCA）分别评估对照组和病例组伤后1个月时的认知功能;采用酶联免疫吸附试验测定2组研究对象的血清BDNF水平;聚合酶链反应-限制性片段长度多态性分析BDNF Val66Met基因多态性;多元逐步回归法分析病例组整体认知功能与BDNF及BDNF Val66Met基因多态性的相关性。 结果病例组伤后1个月相同基因亚型血清BDNF浓度均低于对照组,差异有统计学意义（P<0.05）;病例组Val/Val亚型血清BDNF浓度高于Val/Met、Met/Met亚型,差异有统计学意义（P<0.05）,而Val/Met和Met/Met亚型血清BDNF浓度比较差异无统计学意义（P>0.05）。病例组患者3种基因亚型伤后1个月的LOTCA和MoCA评分均低于对照组,差异有统计学意义（P<0.05）;病例组Val/Val亚型评分高于Val/Met、Met/Met评分,差异有统计学意义（P<0.05）,而Val/Met和Met/Met亚型评分比较,差异无统计学意义（P>0.05）。DAI（Ⅱ型）整体认知水平与BDNF Val66Met基因多态性、BDNF浓度具有线性回归关系（F=11.417,P<0.001）,其具有一定的相关性（|β|=0.966、0.877;r=0.569、0.579）。 结论BDNF可影响DAI认知功能,其BDNF Val66Met基因多态性可能是影响DAI认知功能的风险因素之一。     

n-HA/PA66复合生物活性人工椎体支撑植骨治疗胸腰椎爆裂型骨折合并截瘫

目的:探讨用前路椎管减压、自行研制的纳米羟基磷灰石/聚酰胺66(n-HA/PA66)复合生物活性人工椎体支撑植骨治疗胸腰椎爆裂型骨折合并截瘫的临床疗效。方法:2003年12月—2006年1月收治胸腰椎爆裂型骨折合并截瘫40例,年龄17～62岁。受伤部位:T_(12)5例、L_1 16例、L_2 13例、L_3 6例。神经损害按Frankel分级:A级7例、B级19例、C级8例、D级6例。所有病例均行前路椎管减压、n-HA/PA66复合生物活性人工椎体支撑植骨、钛钉板或钉棒系统内固定。结果:所有病例术后均获得6～25个月(平均13个月)的随访。神经功能:除5例Franel A级和2例D级无变化外,其余均有1～2级的改善。其中由A级到B级2例;B级到C级7例,B级到D级12例;C级到D级5例,C级到E级3例;D级到E级4例。36例伤椎接近解剖复位,6例复位良好。术前伤椎前缘高度平均为椎体的42.8%,术后平均为90.5%,重建的椎体高度无丢失。矢状面Cobb角术前平均为28.4°,术后平均为14.6°。n-HA/PA66复合生物活性人工椎体于术后3～6个月骨性融合。无感染、无椎体移位和断钉等并发症。结论:前路n-HA/PA66复合生物活性人工椎体支撑植骨能有效恢复和维持伤椎的结构和高度,可避免取自体髂骨植骨,是一种有效的脊柱前路重建方法。     

后交叉韧带重建中转角效应：力学机制与临床改良

目的总结后交叉韧带（posterior cruciate ligament，PCL）重建中转角效应的研究进展。方法查阅近年来国内外 PCL 重建中转角效应相关研究，并进行总结。结果研究表明转角效应是导致 PCL 重建术后移植物松弛的主要原因。目前临床上主要通过改变骨隧道固定方式、改变骨隧道方位以及挤压螺钉辅助固定、保留韧带残端、打磨骨隧道口骨质等方式来减小该效应，但缺乏长期临床随访结果。结论针对转角效应的改进方法仍存在较大争议，需要更深入的基础研究，进一步探索重建后 PCL 磨损机制。     

化学萃取同种异体肌腱联合同种异体软骨细胞重建兔肩关节前盂唇实验研究

目的研究化学萃取同种异体肌腱移植联合注射同种异体软骨细胞重建兔肩关节前盂唇损伤的效果。方法成年新西兰大白兔 45 只，体质量 2.5～3.0 kg。取其中 15 只兔的跟腱，采用化学萃取法进行抗原灭活制备同种异体肌腱，将萃取后肌腱与未处理肌腱行 HE 染色和 Masson 染色对比；取膝关节软骨，采用胰蛋白酶法分离培养软骨细胞并行Ⅱ型胶原免疫组织化学染色鉴定。取剩余 30 只兔制备肩关节前盂唇缺损模型，将同种异体肌腱移植至受损盂唇后，随机分为两组（每组 15 只），A 组移植术后即刻关节内注射同种异体软骨细胞，B 组不作任何处理。术后 4、6、8 周每组各取 5 只兔的移植肌腱，大体观察后采用 HE 染色观察细胞核数量，Masson 染色观察肌纤维组织胶原纤维的表达情况，AB 染色检测移植后糖胺聚糖含量，评估两组同种异体肌腱组织内细胞生长情况。结果HE 染色、Masson 染色显示，采用化学萃取法制备的同种异体肌腱抗原被灭活且纤维组织结构保持完好；Ⅱ型胶原免疫组织化学染色示，培养细胞为软骨细胞。肌腱移植术后 AB 染色示，各时间点 A 组糖胺聚糖含量均显著高于 B 组，差异有统计学意义（P<0.05）。术后 6 周 HE 染色示，A 组肌腱组织内的细胞核数量明显多于 B 组（t=20.043，P=0.000）。术后 6 周 Masson 染色示，A 组肌腱组织中的细胞核明显增多，肌纤维及胶原纤维相互交错，组织结构更加紧凑，肌腱组织以蓝染为主；而 B 组细胞核较少，主要为原移植物的胶原纤维。 结论经化学萃取法灭活抗原的同种异体肌腱，能够修复重建兔肩关节前盂唇缺损，且联合同种异体软骨细胞移植能够促进移植肌腱的愈合。