Expression profile of receptors for myelin-associated inhibitors of axonal regeneration in the intact and injured mouse central nervous system

Although CNS neurons have the potential to regenerate their axons after injury, myelin debris carrying axon growth inhibitors rapidly induce growth cone collapse. Receptors (NgR1, NgR2) and coreceptors (LINGO-1, p75(NTR), TROY) for these inhibitors have been characterized and transduction pathways partially identified. However, little is known about the expression of these receptors in intact and lesioned supraspinal projection neurons. Using in situ hybridization, immunohistochemistry and neuronal tract-tracing, we found that NgR1, NgR2 and LINGO-1 are strongly expressed in several neuronal populations of the adult mouse brain projecting to the spinal cord, including neurons projecting through the corticospinal, rubrospinal, caerulospinal, reticulospinal, raphespinal and vestibulospinal tracts. As expected, p75(NTR) expression was restricted to neuronal descending pathways from the brainstem. TROY was absent from most brain regions and from all neuronal projection systems, suggesting that additional signal-transducing coreceptors exist. Qualitative and quantitative analyses revealed that brain expression for these receptors was not affected by a severe T10 spinal cord contusion.     

纳米羟基磷灰石/聚酰胺66骨填充材料与自体骨修复良性骨肿瘤术后骨缺损的对比研究

目的比较纳米羟基磷灰石/聚酰胺66骨填充材料与自体骨修复良性骨肿瘤术后骨缺损的临床效果。方法 2007年1月-2009年3月,选取52例良性骨肿瘤患者随机分成A、B两组,行肿瘤刮除术,A组瘤腔用颗粒型及条形纳米羟基磷灰石/聚酰胺66骨填充材料填充,B组用自体骨填充,伤口常规缝合;比较两组手术时间、术中失血量、术后血沉及C反应蛋白变化情况,评价骨缺损愈合情况。结果纳米羟基磷灰石/聚酰胺66材料填充组与自体髂骨填充组比较,手术时间明显缩短（P〈0.01）,失血量明显减少（P〈0.01）,两组对修复良性骨肿瘤术后骨缺损疗效相当。结论纳米羟基磷灰石/聚酰胺66骨填充材料是修复良性骨肿瘤术后骨缺损的优良材料。     

聚酰胺树脂纯化油菜花粉黄酮部位工艺研究

目的研究聚酰胺树脂精制纯化油菜花粉抗前列腺增生黄酮部位工艺及参数。方法以总黄酮质量分数为考察指标,通过不同条件下聚酰胺树脂对油菜花粉黄酮静态和动态吸附与解吸特性的研究,确定聚酰胺树脂对油菜花粉抗前列腺增生黄酮部位的最佳精制工艺;采用优选的最佳精制工艺对油菜花粉黄酮粗提物进行多次精制,得到高质量分数的油菜花粉抗前列腺增生的黄酮部位。结果在室温和pH值为10.0,油菜花粉黄酮粗提物质量浓度为8 mg/mL时,以80%乙醇1.5 mL/min体积流量洗脱效果最佳。结论油菜花粉黄酮粗提物经过聚酰胺树脂精制纯化后黄酮的质量分数由粗品的24.65%提高到74.90%,提高了2.03倍,说明聚酰胺树脂精制纯化油菜花粉抗前列腺增生黄酮部位是可行的。     

骨髓间充质干细胞与支架材料nHA/PA66复合培养的生物活性研究

目的：研究骨髓间充质干细胞与支架材料-纳米羟基磷灰石/聚酰胺66（nHA/PA66）复合培养时生物学特性。方法：体外培养兔骨髓间充质干细胞（MSCs）,并分为A、B、C、D四组。A组为对照;B组和D组进行骨向诱导;同时,C组和D组细胞与nHA/PA66复合培养。应用MTT实验、碱性磷酸酶（ALP）染色和生物化学分析、扫描电镜,分别对各组细胞的增殖速度、ALP活性及细胞在支架上的黏附情况进行检测。结果：MTT结果显示,A组细胞增殖曲线与C组相似,而B组和D组相似;A组和C组细胞增殖略高于B组和D组。结果表明,骨向诱导可使细胞增殖轻微下降,而与支架材料复合培养对MSCs增殖无影响。ALP染色及ALP活性生化分析进一步表明,MSCs与支架材料复合培养对其骨向分化能力无影响。SEM观察则表明,C组细胞和D组细胞与支架材料均粘附良好。结论：nHA/PA66适于MSCs的黏附、增殖及骨向分化,是一种极具价值的骨组织工程支架材料。     

188Re anti‐CD66 radioimmunotherapy combined with reduced‐intensity conditioning and in‐vivo T cell depletion in elderly patients undergoing allogeneic haematopoietic cell transplantation

The addition of radioimmunotherapy to conventional and reduced‐intensity conditioning has been shown to be feasible and effective. Within an ongoing prospective phase II trial, 22 patients with advanced myeloid malignancies and a median age of 65 years (range 54–76) received anti‐CD66 Rhenium radioimmunotherapy followed by fludarabine (150 mg/m²), busulfan (8 mg/kg) and alemtuzumab (75 mg) before allogeneic haematopoietic stem cell transplantation from matched sibling (n = 7) and unrelated donors (n = 15). The extramedullary toxicity in the first 100 d post‐transplantation was limited and all patients engrafted with complete donor chimaerism. The incidence of non‐relapse mortality at day 100 and after 2 years was 4·5% and 23%, respectively. The probability of overall survival at 2 years was 40%. A comparison with a younger historical cohort (median age 57 years) having received the same dose of fludarabine and busulfan but neither radioimmunotherapy nor alemtuzumab showed no difference in outcome. Although the use of alemtuzumab reduced the incidence of acute graft‐versus‐host‐disease, it was associated with a relapse incidence of 40% despite the incorporation of radioimmmunotherapy. In summary, we confirmed the feasibility of combined radioimmunotherapy and reduced‐intensity conditioning in elderly patients. Further optimisation, probably involving less T cell depletion, is necessary before a randomized comparison with standard conditioning can be planned.     

纳米羟基磷灰石对软化牙本质再矿化效果实验研究

目的研究纳米羟基磷灰石对软化牙本质再矿化的效果。方法采用人工添龋模型,将纳米羟基磷灰石和氢氧化钙作为垫底材料,置于模型中的软化牙本质上,在人工唾液恒温水浴,显微硬度计测定标本垫底1个月、2个月以及3个月后的硬度值,观察标本表面显微硬度的变化。结果纳米羟基磷灰石能明显提高软化牙本质的表面显微硬度,同时随着盖髓时间的增长再矿化效果明显增加。结论纳米羟基磷灰是一种具有良好牙本质再矿化作用的盖髓剂。     

牙体修复性纳米羟基磷灰石复合材料的机械性能研究

目的 本研究目的 旨在研制一种能达到牙齿仿生学要求并用于牙科修复的生物活性材料.方法 将经过硅烷偶联剂KH-570进行表面处理后的纳米羟基磷灰石(Nano-hydroxyapatite,NHA)与配制好的光固化树脂基质共混,制备成复合材料,根据复合材料中NHA所占的不同比例(45wt%、50wt%、55wt%、60wt...     

功能矫治器前伸下颌后SD大鼠咀嚼肌中Ⅳ型胶原mRNA表达水平的改变

目的：通过检测大鼠下颌前伸后咀嚼肌中Ⅳ型胶原的表达水平的改变，研究颌骨矫形过程中咀嚼肌适应性变化及其规律，并初步探讨其适应性变化机制，为矫形治疗提供新的理论依据。方法：选用40只4周龄雄性Sprague—Dawley（SD）大鼠为实验对象，用自制的功能矫治器斜导导下颌向前。采用RT-PCR法对Ⅳ型胶原mRNA进行测定。结果：实验组Ⅳ型胶原的表达随着时间的变化出现先增加后减少再增加的表现，实验组Ⅳ型胶原的表达较实验组整体减少。结论：Ⅳ型胶原介导了组织重构过程，为MMPs、TIMPs的平衡理论提供了新的依据，即MMPs／TIMPs处于一个平衡-改变-平衡这样一个动态的平衡当中。     

Raji肿瘤细胞的氨基酸代谢

研究Ｒａｊｉ肿瘤细胞氨基酸代谢的初步规律。方法收集培养过程听Ｒａｊｉ肿瘤细胞冻融，制备我蛋白细胞滤液，采用聚酰胺薄层层析法测定Ｒａｊｉ肿瘤细胞游离基酸含量。结果细胞培养至４８ｈ时，酪氨酸、等的浓度升至最高峰。羟脯氨酸，谷氨酸和天门冬氨酸浓度无改变。     

Molecular analysis of HLA DR4-/β1 gene in malaria vaccinees. Typing and subtyping by PCR technique and oligonucleotides

The combination of the PCR technique and the synthetic oligonucleotides has proved to be a useful tool in the molecular analysis of HLA class II genes, allowing recognition of as little as a single nucleotide modification in the sequence of the gene. The molecules encoded by these genes have been associated with genetic control of the immune response and with susceptibility to certain diseases. Studies carried out in our laboratory have shown three patterns of humoral immune response in the human volunteers vaccinated with the synthetic protein SPf 66; high, intermediate and low responders. Approximately 73.3% of the low responders were serologically typed as HLA DR4 and 42% as DQw6. These results moved us to look for a subtype (Dw) correlation between the DR4 positive individuals and the different humoral immune response patterns. Using oligo-typing methods after previous amplification of the DR4 B1 exon, we subtyped 20 DR4 volunteers, classified as high, intermediate and low responders. We did not find any direct association between the HLA DR4 Dw special subtype in the high or low responders immunized with the SPf 66 vaccine.