体针联合静滴康体多治疗泛发性神经性皮炎50例

目的:观察体针联合康体多治疗神经性皮炎的的疗效。方法:86例患者随机分为两组,治疗组采用体针针刺(双合谷、曲池、三阴交等)联合静滴康体多注射液,对照组单纯采用静滴康体多注射液。结果:治疗组总有效率100%,对照组88.9%,有显著性差异(P<0.05)。提示:本方法具有活血通经功效,可使肥厚苔藓化的皮损逐步变得光滑有弹性。     

脾切除贲门周围血管离断术后肝脏血流动力学和储备功能的变化

Objective To observe changes of hepatic hemodynamics and hepatic functional reserve after splenectomy and periesophagogastric devascularization. Methods From July 2006 to August 2007, thirty patients with portal hypertension caused by hepatitis B cirrhosis underwent splenectomy and periesophagogastric devascuiarization. The PVPG (portal venous pressure gradient) was measured by inductor continually during operation. The HAF (hepatic artery flow), PVF (portal venours flow) and hepatic arterial RI(resistant index) were measured with Doppler sonography. The EHBF(effective hepatic blood flow) and ICGR15 (indocyaninegreen retention rate at 15 minutes) were obtained respectively by indocyaninegreen clearance test before and after the operation. Results PVPG after laparotomy (19±4) mm Hg, ligating the splenic artery(14±4) mm Hg, splenectomy(14±3)mm Hg and periesophagogastric devascularization (12±4) mm Hg showed a tendency to decrease progressively. The PVF decreased [from (42±14) ml/s to (16±8) ml/s] and HAF increased in compensation after operation. The EHBF increased [from (0.48±0.10) L/min to (0.56±0.10) L/min], and the ICGR15 decreased (from 23%±8% to 18%±4%) postoperatively. Conclusion After splenectomy and periesophagogastric devascularization, the hepatic functional reserve improves at least in a short term notwithstanding the decrease of PVPG and PVF.     

细胞因子对多器官功能不全综合征大鼠结肠运动的影响

机体发生多脏器功能不全综合征(MODS)后,白介素 6(IL 6 )、肿瘤坏死因子 α(TNF α)和一氧化氮(NO)水平升高,而结肠运动减弱;我们对二者之间的内在联系进行了初步的研究。材料与方法1.材料:Wistar大鼠体重2 5 0 g左右(军科院动物实验中心)。Biopacsrsremmp15 0型多导生理仪,诱生性一氧化氮合酶(iNOS)、TNF α和IL 6免疫组化试剂盒(中山公司,北京) ,总RNA提取试剂(Trizol,美国Gibico公司)、逆转录聚合酶联反应(RT PCR)试剂盒(美国Promega公司)、iNOS、TNF α和IL 6及βactin引物由上海生工合成。2 .方法:(1)模型制备及分组:…     

用双腔心脏起搏器治疗缓慢性心律失常10例

临床资料研究表明,缓慢性心律失常导致症状的主要原因为心动过缓:(1)阿-斯综合征;(2)头昏、乏力、黑朦等全身症状;(3)心衰加重[1].     

Rosiglitazone down-regulates lipopolysaccharide-induced expression of CD40 and intercellular adhesion molecule 1 in rat peritoneal mesothelial cells through a NF-κB dependent mechanism

Objective To investigate the effect and mechanism by which PPARγ ligand, rosiglitasone, regulates the expression of CD40 and intercellular adhesion molecule 1 (ICAM-1) in the rat peritoneal mesothelial cells (RPMCs) induced by lipopolysaccharide (LPS). Methods RPMCs were harvested from Sprague-Dawley rat peritoneal cavity and maintained under defined in vitro conditions. The cells were randomly divided into groups as follows: medium, LPS (5 mg/L), LPS (5 mg/L)+BAY11-7085(5 μmol/L, NF-κB inhibitor), rosiglitazone (10 μmol/L or 20 μmol/L, peroxisome proliferator-activated receptor γ activator), LPS (5 mg/L)+rosiglitazone (10 μmol/L)+GW9662 (3 μmol/L, peroxisome proliferator-aetivatcd receptor γ antagonist), and LPS (5 mg/L)+vehicle (DMSO 0.2 ml/L). The expressions of CD40 and ICAM-1 RNA in RPMCs were examined by RT-PCR after 3 hour treatment, and the protein expressions of CD40, ICAM-1, p-NF-κB p65 and p-IκBα were examined by Western blot or immunofluorescence after 24 hour treatment. Results Following treatment with LPS, both the expressions of CD40 and ICAM-1 protein in RPMCs were up-regulated significantly (P<0.05), and the phosphoralation of p65 was increased greatly (1.10±0.17 vs 0.55±0.06, P<0.05). BAY11-7085 (5 μmol/L) significantly decreased the protein expression of p-p65 (0.22±0.11 vs 1.10±0.17, P<0.01), CD40 (0.34±0.02 vs 0.50±0.06, P<0.05) and ICAM-1 (0.35±0.16 vs 0.74±0.03, P<0.05). Pretreated with rosiglitazone for 3 h then added with LPS for 1 h, the levels of p-p65, CD40 and ICAM-1 in RPMCs were significantly decreased compared with those of LPS group (0.77±0.08 vs 0.90±0.10, P相似文献   

一期后前路手术治疗多节段脊髓型颈椎病

目的 探讨治疗多节段重度脊髓型颈椎病的手术方式及疗效。方法 采用一期后路“双开门”椎板成形、侧块钛板固定加前路减压支撑植骨钛板固定术治疗术前MRI显示三个节段以上(含三个节段)颈脊髓前后方受压，X线动态片显示颈椎失稳或后突畸形的20例脊髓型颈椎病。结果 一期后、前路手术20例，手术时间平均5．5h(5～7h)，失血量平均500ml。术后1～2d病人戴颈围离床活动，颈围外固定12周，所有病例随访6～18个月(平均10个月)，无伤口感染，未见颈椎内固定及植骨块松脱移位，X线摄片显示术后4个月前方植骨块与椎体融合。临床颈痛消失，四肢神经症状按Nurick分级均较术前上升一级以上，平均从术前3．3级升至术后2．4级。结论 一期后、前路颈椎管减压、内固定、植骨融合术为复杂棘手的多节段的脊髓型颈椎病手术治疗提供了一种比较安全、可靠的方法。在达到充分解除颈脊髓前后压迫同时稳定颈椎，预防了前方支撑植骨的松脱、颈椎的后突畸形发生，提高了植骨融合成功率。     

姬松茸多糖的抗炎作用

[目的 ]观察姬松茸多糖的抗炎作用 .[方法 ]制作二甲苯所致小鼠耳急性炎症模型、角叉菜胶所致大鼠关节肿胀模型、小鼠棉球肉芽肿亚急性炎症模型和大鼠佐剂关节炎模型 ,给予 4 0 ,80 ,16 0mg/kg的姬松茸多糖 .[结果 ]姬松茸多糖明显抑制上述各类型炎症反应 .[结论 ]姬松茸多糖具有抗炎作用 .     

2型糖尿病低血糖昏迷15例临床分析

目的　探讨 2型糖尿病发生低血糖昏迷的临床特点。方法　对 15例患者的临床资料进行回顾性分析。结果　 2型糖尿病低血糖昏迷高发于 60岁以上年龄大、病程长的患者 ,以使用磺脲类药物和胰岛素者多见。快速进展的低血糖脑病为其主要临床表现。经积极治疗 ,预后良好。结论　老年 2型糖尿病患者应坚持专科治疗 ,合理用药 ,监测血糖 ,并了解低血糖的常见原因和急救措施 ,可望预防低血糖昏迷的发生。     

携带人胰岛素样生长因子-1重组腺病毒的构建和鉴定

[目的] 采用 Cre-LoxP同源重组系统构建并鉴定携带人胰岛素样生长因子-1( human insulin-like growth factor-1, hIGF-1)目的基因的复制缺陷重组腺病毒,为椎间盘退变的 hIGF-1基因治疗奠定基础.[方法] PCR合成 hIGF-1基因,用 pMD18-T载体克隆 hIGF-1目的基因,酶切、测序并进行 NCBI BLAST相似性在线分析.将 pMD18-T-hIGF-1和 pDNR-1r质粒分别行 EcoRⅠ和 BamHⅠ双酶切, DNA 连接酶连接双酶切产物,转化感受态大肠杆菌 DH5α,合成中间载体 pDNR-hIGF-1,酶切鉴定.Cre-loxP系统介导 pDNR-hIGF-1和 pInt.AV1.SpaT同源重组形成 pInt.AV1.SpaT-hIGF-1重组表达质粒,行 EcoRⅠ酶切鉴定.复苏 293细胞,将 pInt.AV1.SpaT-hIGF-1和 pInt.B.B质粒在 293细胞内进行同源重组成含 hIGF-1基因的复制缺陷重组腺病毒.倒置显微镜观察 293细胞病变样效应( cytopathogenic effect, CPE);透射电镜观察 293细胞中的病毒颗粒;提取细胞裂解液中病毒 DNA, PCR鉴定 hIGF-1目的基因的存在; Western blot 检测 hIGF-1蛋白表达.用半数组织培养感染量 (tissue culture infectious dose 50,TCID50)方法测定重组腺病毒的滴度.[结果]克隆的 hIGF-1目的基因经 NCBI BLAST相似性在线分析证实与 Homo sapiens IGF-1 (GI:19923111)完全相同.pInt.AV1.SpaT-hIGF-1酶切鉴定,出现 5.4 kb和 1 kb两条片段,与理论值完全相符.转染 293细胞 12～ 14 d后,大部分细胞出现肿胀,脱落等细胞病变样效应.PCR鉴定细胞裂解液中含有 hIGF-1目的基因.Western blot 证实重组腺病毒表达 hIGF-1蛋白.第 2代腺病毒的滴度为 80× 106 PFU/mL.[结论]成功构建出含有 hIGF-1目的基因的复制缺陷重组腺病毒.     

中等剂量静脉注射免疫球蛋白治疗成人慢性免疫性血小板减少性紫癜

[目的]探讨中等剂量免疫球蛋白在治疗免疫性血小板减少性紫瘢的疗效.[方法]治疗组21例采用中等剂量免疫球蛋200mg(kg·d),对照组19例为400mg/(kg·d)静脉滴注,均连续5天,观察血小板峰值,峰值持续时间,下降程度.[结果]治疗组21例中完全反应8例,部分反应7例,无效6例,总有效率为71.3%.对照组19例中完全反应8例,部分反应6例,总有效率为73.5%.从两组血小板峰值,峰值持续时间,峰值后血小板下降程度等比较无统计学差异(P>0.05).[结论]中等剂量与大剂量免疫球蛋白同样有效,且可降低费用,值得临床推广使用.