白花前胡对肺动脉高压大鼠肺循环血液流变学及血流动力学的影响

目的评价白花前胡对慢性炎症性肺动脉高压的防治效果,探讨肺循环血液流变学特征的改变在肺动脉高压形成中的作用.方法采用野百合碱(monocrotoline,MCT)诱导的慢性炎症性肺动脉高压大鼠模型;白花前胡(50,100,150mg·kg     

管花马兜铃导致大鼠急性肾损伤的实验研究

目的观察管花马兜铃对大鼠急性肾损伤的作用. 方法用管花马兜铃水煎剂(15g*kg-1*d-1)对大鼠灌胃,建立大鼠急性肾损伤模型,检测有关肾功能指标,进行组织形态学观察.结果大鼠肾脏的主要功能改变为多尿、氮质血症、蛋白尿及血尿.组织形态学改变主要表现为急性肾小管变性、坏死.结论实验剂量的管花马兜铃可导致大鼠急性肾功能衰竭.     

玉郎伞的显微和紫外吸收光谱鉴定

目的为玉郎伞鉴别和开发利用提供依据.方法显微鉴定和紫外吸收光谱.结果发现玉郎伞有明显的鉴别特征根的横切面中皮层有石细胞群和纤维束,断续成环;粉末中晶鞘纤维、具缘纹孔导管多见;粉末乙醇液在261、216、204±1nm波长处有吸收峰,而氯仿液在262±1nm波长处有吸收峰.结论以上特征可作为鉴别玉郎伞的依据.     

鸡血藤复方治疗再生障碍性贫血66例30年随访

目的 评价再生障碍性贫血（简称再障）经鸡血藤复方治疗后的长远期结局。 方法 采用通信联系早年（1976—1994年）鸡血藤复方及丙酸睾酮（阳虚时加用）治疗有效的110例再障患者,收集其目前健康、工作状况,复发和并发症。同时将获得信息反馈的66例患者与近期初诊的13例再障患者以及36名健康人作了对比。结果 获得66例患者的信息反馈,66例平均血细胞计数：WBC 4.7×10⁹/L, N（ 中性粒细胞计数）2.5×10⁹/L, Hb 121.2 g/L, 血小板（BPC） 109.5 ×10⁹/L, 骨髓造血组织平均72.7%,与初诊组比较差异均有统计学意义（P＜0.01）;SF-36积分与健康人比较差异无统计学意义（P>0.05）。66例随访者中处于学龄期30例,接受教育17例（56.7%）;治疗后结婚24例,加上原已成家的26例和8例在校学生,治疗后家庭完善率达87.9%（58/66）;参加工作43例（65.2%）。死亡4例;复发7例（10.6%）;合并症5例（7.6%）。 结论 再障可以用鸡血藤复方及丙酸睾酮治愈,有很好的药物安全性。患者治愈后可完全融入社会,过着正常人的生活与工作。     

Double plication for spring-mediated in-continuity intestinal lengthening in a porcine model

Background

Short bowel syndrome is a condition with substantial morbidity and mortality, yet definitive therapies are lacking. Distraction enterogenesis uses mechanical force to “grow” new intestine. In this study, we examined whether intestinal plication can be used to safely achieve spring-mediated intestinal lengthening in a functioning segment of jejunum in its native position.

白花前胡水提物对阿霉素在大鼠体内药动学的影响

目的:研究白花前胡水提物对阿霉素在大鼠体内的药物动力学的影响。方法:将SD大鼠随机分为两组:阿霉素组,阿霉素+白花前胡水提物组,采用HPLC法测定阿霉素在不同时间点的血药浓度,3P97程序计算药动学参数。结果:阿霉素+白花前胡水提物组与阿霉素组的药动学参数相比,AUC显著增大(P<0.05),同时CL显著减小(P<0.05),其余参数差异无统计学意义(P>0.05)。结论:白花前胡水提物可使阿霉素在大鼠体内的血药浓度增加,提高其生物利用度。     

Antiplatelet effects of Spatholobus suberectus via inhibition of the glycoprotein IIb/IIIa receptor

Ethnopharmacological relevance

The vine stem of Spatholobus suberectus is a widely used blood-activating and stasis-dispelling medicine for the treatment of diseases related to blood stasis syndrome in traditional medicine in Korea, Japan, and China.

Aim of the study

To demonstrate the clinical effects of Spatholobus suberectus against blood stasis syndromes using in vitro and in vivo platelet aggregation studies and to investigate its exact mechanisms.

Materials and methods

We extracted vine stems of Spatholobus suberectus, using 95% EtOH (SSE) and investigated its antiplatelet activity on platelet aggregation induced by collagen and ADP in human platelet-rich plasma (PRP). For the mechanism study, a glycoprotein IIb/IIIa (GP IIb/IIIa) assay using flow cytometric analysis and a thromboxane A₂ (TXA₂) assay were performed. In addition, we investigated the effects of SSE in a thromboembolic mouse model.

Results

SSE significantly inhibited ADP- and collagen-induced platelet aggregation in human PRP concentration-dependently without affecting plasma clotting time. It also significantly inhibited fibrinogen binding to the GP IIb/IIIa receptor and partly inhibited the formation of TXA₂. In the in vivo study, oral administration of SSE dose-dependently suppressed the death of thromboembolism model mice induced by intravenous injection of collagen plus epinephrine.

Conclusions

SSE showed antiplatelet activity without anticoagulant effects mainly through the inhibition of fibrinogen binding to the GP IIb/IIIa receptor. Our current results support the clinical usage of SSE in the East Asian region treating atherothrombotic diseases and may represent a new natural source to develop antiplatelet agents.     

Spatholobus suberectus inhibits cancer cell growth by inducing apoptosis and arresting cell cycle at G2/M checkpoint

Aim of the study

Although herbs have long been alternatively applied for cancer treatment in China, its treatment effects and their potential mechanisms have not been sufficiently investigated. The chinese herb Spatholobus suberectus (SS) is commonly prescribed to cancer patients. In this study, the anti-cancer effect of SS and its molecular mechanisms have been investigated.

Materials and methods

The effect of SS on cell proliferation was studied by cell growth assay and flow cytometry on breast cancer cell lines MCF-7 and colon cancer cell line HT-29. The role of SS in apoptosis was studied by flow cytometry, DNA fragmentation assay and mitochondrial membrane potential assay. Expression of proteins associated with cell cycle and apoptosis was determined by Western blot analysis. The in vivo effect of SS was tested in nude mouse cancer xenografts.

Results

Cell growth assay showed that SS effectively inhibits tumor cell growth in a dose-dependent manner. Flow cytometry analysis showed that SS could arrest the cell cycle at G2/M checkpoint, which is associated with DNA damage and activation of phosphor-Chk1/Chk2. The pro-apoptotic effect of SS was demonstrated by Annexin V-PI staining and mitochondrial membrane potential assay. In vivo experiments show that the efficiency of SS alone group was superior to docetaxel or to docetaxel and SS combined. No obvious body weight loss or blood toxicity was observed in SS tested animals.

Conclusions

Our data demonstrates that SS is a potential herb for cancer treatment by inhibiting tumor growth via induction of apoptosis and arrest of the cell cycle at G2/M phase.     

长柄紫珠化学成分研究

目的 系统研究长柄紫珠Callicarpa longipes的化学成分,为先导化合物的发现及质量评价提供基础.方法 采用反相硅胶、Sephadex LH-20、Toyopearl HW-40等柱色谱技术,结合重结晶进行分离纯化,光谱及核磁共振技术结合化学反应鉴定结构.结果 分离并鉴定了12个化合物,分别为熊竹山姜素(1)、5,4'-二羟基-3,6,7-三甲氧基黄酮(2)、阿亚黄素(3)、槲皮素-3,3',4'-三甲醚(4)、5,7,3',4'-四羟基-3-甲氧基黄酮(5)、芹菜素(6)、槲皮素-3,3'-二甲醚(7)、芹菜素-6,8-二-C-β-D-葡萄糖苷(8)、山柰酚-7-O-β-D-葡萄糖苷(9)、白桦脂酸(10)、熊果酸(11)、β-谷甾醇(12).结论 12个化合物均为首次从该植物中分离得到,其中化合物1～9为黄酮类,化合物2～5、7～9为首次从紫珠属中分离得到.     

不同溶剂对鸡血藤提取物总黄酮含量 及抗肿瘤活性的影响

目的:探讨不同溶剂对鸡血藤提取物中的总黄酮含量和抗肿瘤活性的影响。方法:分别用不同浓度的乙醇水溶液、丙酮水溶液作为提取溶剂,利用回流法、超声提取法制备鸡血藤提取物;采用四甲基噻唑蓝(MTT)法测定提取物对结肠癌HT-29细胞增殖的抑制活性;采用分光光度法测定提取物中总黄酮含量;采用HPLC法测定提取物中芒柄花素含量。结果:60%乙醇回流60 min所得提取物总黄酮含量最高并且对HT-29细胞增殖的抑制活性最强。结论:鸡血藤抗肿瘤活性提取物的制备以60%乙醇回流提取为佳。