人参皂苷Rb1对体外培养人脂肪干细胞增殖与成骨分化的影响

背景：脂肪干细胞成骨分化受多种因素的影响,中药成骨诱导活性因子在脂肪干细胞研究中有重要意义。 目的：观察人参皂苷Rb1在体外培养条件下对人脂肪干细胞增殖和成骨分化的影响。 方法：体外分离培养人脂肪干细胞,传至第3代后,按2×10³/孔接种至96孔板,分别加入0.5,1.0,2.0,4.0,6.0 μmol/L人参皂苷Rb1培养基200 μL进行培养;设立对照组,仅加入等量普通DMEM培养基。采用XTT比色法测定大鼠脂肪干细胞的生长增殖曲线。通过碱性磷酸酶试剂盒测定细胞碱性磷酸酶活性,放射免疫法检测骨钙素含量,茜素红染色观察钙化结节形成能力。 结果与结论：0.5 μmol/L人参皂苷Rb1可明显促进人脂肪干细胞增殖;随着人参皂苷Rb1浓度的增加,促细胞增殖活性降低,6.0 μmol/L人参皂苷Rb1表现为明显的抑制细胞增殖作用。人参皂苷Rb1呈剂量依赖性促进人脂肪干细胞碱性磷酸酶活性和骨钙素表达。4.0,6.0 μmol/L人参皂苷Rb1诱导钙化结节形成能力优于0.5,1.0和2.0 μmol/L人参皂苷Rb1,对照组人脂肪干细胞未见钙化结节形成。提示人参皂苷Rb1在一定浓度范围内对体外培养条件下的人脂肪干细胞具有促生长增殖作用,但在高浓度时,人参皂苷Rb1对人脂肪干细胞的成骨分化具有促进作用,因此可作为一种良好的成骨诱导活性因子。     

肝细粒棘球蚴外囊壁钙化相关受体

目的 比较肝细粒棘球蚴病患者钙化外囊壁及非钙化外囊壁上的钙化相关受体BMPRⅡ(骨形态发生蛋白Ⅱ型受体)、IGF1R(胰岛素样生长因子1受体)和ERα(雌激素受体α)的表达差异。方法 钙化外囊壁和非钙化外囊壁茜素红染色,Envision免疫组化法和qRT-PCR分别检测同一细粒棘球蚴病患者钙化外囊壁及非钙化外囊壁上钙化相关受体BMPRⅡ、IGF1R和ERα的表达水平和钙化相关受体的mRNA表达量。结果 与细粒棘球蚴非钙化外囊壁相比较,同一患者钙化外囊壁茜素红染色钙化显著,且差异有统计学意义(χ²=20.369,P<0.01);钙化外囊壁相关受体的表达明显增加,且差异有统计学意义(P<0.05),mRNA表达量明显增高且差异具有统计学意义(P<0.05)。结论 肝细粒棘球蚴病患者钙化外囊壁钙化相关受体表达量较高,钙化相关因子通过与受体BMPRⅡ、IGF1R和ERα等结合,引起细粒棘球蚴外囊壁钙化,外囊壁的钙化可以有效地抑制细粒棘球蚴的生长,在细粒棘球蚴病患者临床治疗过程中发挥着重要作用。     

The Impact of a Diagnosis of Silicosis

The report of the National Commission on State Workmen’s Compensation Laws, submitted in July 1972, concluded that workmen’s compensation laws are inadequate and inequitable. However, the program should be retained if the most grievous faults are remedied. Eighty-four recommendations for a modern program were made.

The commission felt the federal government could help reform workmen’s compensation. A presidential commission to encourage and assist the states was urged. Congress was asked to review in 1975 the states’ compliance with 19 essential recommendations and guarantee their adoption if necessary. (Three commissioners urged immediate federal action.)

The report was generally endorsed by interest groups. Pending and enacted federal legislation used the report as a guide.

States made numerous changes in their laws this year. Nonetheless, the reform pace would not meet the commission’s 1975 timetable. The test of the report is what happens in the next few years.     

激痛点神经阻滞联合针刀治疗冈上肌腱炎的临床疗效评价

目的:评价激痛点神经阻滞联合针刀治疗冈上肌腱炎的临床疗效。方法:63例患者随机分为"神经阻滞组"32例和"神经阻滞联合针刀组"31例,共2组。取穴:肱骨大结节激痛点和冈上窝激痛点。神经阻滞组于激痛点注射消炎镇痛液;神经阻滞联合针刀组注射消炎镇痛液后,行针刀治疗。1次/周,共3次,3周后随访。结果:2种疗法均能降低VAS评分,提高C-M分值(P0.05)。神经阻滞联合针刀组治疗后VAS=(23.39±22.30)分,C-M=(81.06±15.83)分,优于神经阻滞组(P0.01)。结论:神经阻滞联合针刀组冈上肌腱炎疗效显著。     

A case of persistent subepidermal calcified nodule in an adult treated with CO2 laser

A subepidermal calcified nodule (SCN) is an uncommon form of calcinosis cutis that is not associated with other dermatologic or systemic illnesses. Clinically, SCN presents as a solitary, firm tumor that can be white, white-yellow, or erythematous. Most SCNs occur in children; they are extremely rare in adults. The treatment of choice is believed to be surgical excision. We describe a case of SCN on the right ear lobe of a 33-year-old male, which was treated using a CO2 laser and electrodessication.     

超声引导下肩袖钙化性肌腱炎的穿刺封闭治疗的探讨

目的 探讨在超声引导下肩袖钙化性肌腱炎的穿刺封闭治疗的优点.方法 对我院2012年06月-2012年12月骨科门诊的20例肩袖钙化性肌腱炎患者进行超声引导下穿刺封闭治疗,复查,随诊,并对随访结果进行统计学分析.结果 20例患者均得到随访,患者末次随访进行ASES评分、Constant-Murley评分,2个评分治疗前后相比有统计学差异（P＜0.01）.结论 穿刺封闭治疗是治疗肩袖钙化性肌腱炎的一种安全、有效的方法,在超声引导下实施这一操作,精确点高,注射位置精确.     

火针治疗跟腱炎验案

本文摘录了一则针灸特殊疗法治疗跟腱炎的临床案例,从中可以体会到我国传统针灸疗法的博大精深。     

Proximal aortic surgery in the elderly population: Is advanced age a contraindication for surgery?

Objective

The study objective was to describe the clinical outcomes of elderly patients undergoing ascending aortic surgery.

3-Dimensional Accuracy of Dynamic Navigation Technology in Locating Calcified Canals

IntroductionThis study aimed to present a novel dynamic navigation method to attain minimally invasive access cavity preparations and to evaluate its 3-dimensional (3D) accuracy in locating highly difficult simulated calcified canals among maxillary and mandibular teeth.MethodsThree identical sets of maxillary and mandibular 3D-printed jaw models composed of 84 teeth in their anatomic locations with simulated calcified canals (N = 138 canals) were set up on dental manikins. The Navident dynamic navigation system (ClaroNav, Toronto, Ontario, Canada) was used to plan and execute access preparations randomly with high-speed drills by a board-certified Endodontist. Two-dimensional (2D) and 3D horizontal, vertical, and angulation discrepancies between the planned and placed access preparations were digitally measured using superimposed cone-beam computed tomographic scans. Analysis of covariance models were used to evaluate the associations and the interaction between tooth type and jaw, the canal orifice depth, and the discrepancies between planned and prepared access cavities. The significance level was set at .05.ResultsThe mean 2D horizontal deviation from the canal orifice was 0.9 mm, and it was significantly higher on maxillary compared with mandibular teeth (P < .05). The mean 3D deviation from the canal orifice was 1.3 mm, and it was marginally higher on maxillary teeth in comparison with mandibular teeth (P ≥ .05). The mean 3D angular deviation was 1.7 degrees, and it was significantly higher in molars compared with premolars (P < .05). The 3D and 2D discrepancies were independent of the canal orifice depths (P > .05). The average drilling time was 57.8 seconds with significant dependence on the canal orifice depth, tooth type, and jaw (P < .05).ConclusionsThis study shows the potential of applying dynamic 3D navigation technology with high-speed drills to preserve tooth structure and accurately locate root canals in teeth with pulp canal obliteration.