BHA等试剂对黄曲霉毒素诱发的大鼠骨髓微核的影响

用BHA和PB预先诱导大鼠4天,然后经腹腔给予黄曲霉毒素B_1(AFB_1),给毒后24小时处死大鼠,取股骨骨髓涂片,用Giemsa染色,计算微核率。经诱导后,可使AFB_1诱发的微核率下降约一倍。说明BHA和PB诱导后可降低AFB_1的致突变作用。     

羟基磷灰石眼台植入在眼窝重建中的应用

目的 统计分析501例羟基磷灰石(HA)眼台植入在眼窝重建中的应用。方法局麻或全麻下Ⅰ期植入307例，Ⅱ期植入194例，年龄最大82岁、最小2．5岁。结果480例疗效满意，并发症21例占4，18％。主要有：(1)眼台暴露18例，10例取出；(2)结膜切口裂开11例，7例自行愈合，4例第2次缝合愈合。结论羟基磷灰石由于是人体骨组织的主要无机成分，且无毒，无抗原性，使用安全。     

钚促排药物的设计与合成

利用仿生化学的原理，本文设计并合成了两个儿茶酚胺类和两个羟基吡啶酮类八配位的螯合剂，初步动物试验表明，具有较强的钚促排能力。     

珊瑚人工骨的研究现况

珊瑚的主要成份是碳酸钙,具有类似人类骨骼的多孔结构。近年来人们将其作为一种新型种植材料进行了不少实验和临床应用研究,证实了珊瑚是一种较为理想的生物活性材料。本文就珊瑚的种类、结构与理化特性,珊瑚的生物学特性,实验研究和临床应用作了综述。     

羟基磷灰石复合糊剂用于诱导根尖成形术的临床疗效观察

羟基磷灰石复合糊剂用于诱导根尖成形术的临床疗效观察广西区人民医院黄琦王少英年轻恒牙根尖孔尚未发育完成时如果受到外伤及龋病影响而导致牙髓坏死将影响牙根的继续发育，临床上可采用根尖成形术促进其发育完成（１）。１９９３年～１９９６年我们选用羟基磷灰石加四环...     

羟基磷灰石微粒眶骨膜下植入矫正外伤性眼球内陷二例

羟基磷灰石微粒眶骨膜下植入矫正外伤性眼球内陷二例潘振东，刘德成，王惠珍，于红眼球内陷是眶骨骨折的常见并发症之一。我们自１９９４年来用Ａｌｌｅｎ等［１］方法，于眶底骨膜下植入羟基磷灰石微粒，修复２例眼球内陷，取得了满意效果。现报告如下。一、病例简介例１...     

羟基磷灰石的表面形貌对大鼠骨髓细胞向成骨细胞分化的影响

羟基磷灰石(HA)是一种生物相容性的骨替代材料，不同孔隙率的HA具有不同的表面形貌。本实验旨在研究HA的表面形貌对大鼠骨髓细胞向成骨细胞分化的影响。     

改良以聚羟基乙酸为支架同种异体组织工程化塑形软骨的构建

OBJECTIVE: To improve the method for constructing allogeneic molded cartilage by means of tissue engineering techniques. METHODS: The chondrocytes from the rib and articular cartilage of infant rabbits were harvested by type II collagenase digestion, followed by in vitro cell culture for 3 to 4 passages. The chondrocytes were then prepared into cell suspension and seeded onto C -and O -shaped pre-molded polyglycolic acid (PGA) scaffolds form chondrocyte-PGA composites, which were subsequently cultured in vitro for 7 to 10 d before implanted subcutaneously into adult rabbits. Improvement was made upon conventional shaping and implantation procedures. Morphological observation and cartilage regeneration assessment were conducted at different time points following the implantation, in comparison with the observation by conventional shaping and implantation methods. RESULTS: During in vitro cell culture, the rate of viable chondrocytes in the final cell suspension was (92+/-2)% after well-controlled prolongation of digestion trypsin, similar to the viable cell rate (93+/-2) % by traditional procedures (P>0.05). Gross observation found milk-white, newly generated cartilage which had good flexibility 4 weeks after implantation, and after 8 weeks and later, the cartilage took on the color of porcelain-white. Histological examination showed a few inflammatory cells around the newly generated immature cartilage 4 weeks after implantation, and the inflammation abated when the newly generated cartilage acquired similar histological properties to that of the original cartilage 8 weeks postoperatively and later. After 16 weeks, no blood vessel or capillaries were visible within the new cartilage. CONCLUSION: The chondrocyte viability is not affected when the cells are treated with well-controlled prolonged digestion with trypsin during in vitro cell culture. Improved PGA scaffolds shaping and the implantation procedure facilitate the regeneration of the cartilage after the implantation of the composites.     

10-羟基喜树碱对HepG2体外生长及其小鼠肾包膜下移植瘤的影响

目的　探讨 10 羟基喜树碱 (HCPT)对人肝癌细胞HepG2体外生长及其小鼠肾包膜下移植瘤的影响。方法　用 10 HCPT处理HepG2细胞 ,四甲基偶氮唑蓝 (MTT )法检测其生长指数 (GI) ,软琼脂克隆形成试验检测其克隆形成率 ;建立HepG2小鼠肾包膜下移植瘤模型 ,每天腹腔注射 10 HCPT ,第 6天处死动物 ,测量瘤块体积。结果　 10～ 3 60mg/L浓度 10 HCPT处理组HepG2细胞GI分别为 84.72 %～ 47.65 % ,与对照组比较明显降低 (P <0 .0 5 )。 90～ 3 60mg/L 10 HCPT处理组HepG2软琼脂克隆形成率分别为 3 8.80 %～ 2 5 .2 0 % ,与对照组比较亦明显降低 (P <0 .0 1) ,两者均具有量效依赖关系。 3× 10 -3 ～ 9× 10 -3 mg/3 0 g剂量 10 HCPT处理组小鼠肾包膜移植瘤体积分别为 2 .93～ 1.88mm3 ,与对照组比较明显缩小 (P <0 .0 1)。结论　 10 HCPT对HepG2的体外生长及其小鼠肾包膜移植瘤均具有明显抑制作用。