Short-term survival analysis of the all.polyethylene tibial component in total knee arthroplasty

Toourknowledge, thesurvivorshipofall polyethylenetibialcomponentsintotalkneearthroplastyhasnotbeenstudiedinChinapreviously. Wereportaseriesof24 cementedtotalkneearthroplastyusingall polyethylenetibialcomponents.METHODSBetweenDecember2000andDecember2002, 24cementedtotalkneearthroplastyin21 patientswereperformedusingall polyethylenetibialcomponents.Themeanageofthe21 patients( 9 menand 12women) atoperationwas55 years, ranging48 61years. ThemeanpreoperativeHSSscorewas40. 2(range, 36 43 ).…     

碱性成纤维细胞生长因子缓释微球对雪旺细胞作用的实验研究

目的 探讨碱性成纤维细胞生长因子（bFGF）缓释微球对雪旺细胞的作用。方法 培养雪旺细胞，将bFGF、bFGF-聚乳酸-羟基乙酸共聚物微球（PLGA微球）和bFGF-聚乳酸-聚乙二醇共聚物微球（PELA微球）分别加入三个组的雪旺细胞培养液中。用细胞计数法测定雪旺细胞的数量，四甲基偶氮唑盐微量反应比色法（MTT法）测定雪旺细胞的活力，流式细胞仪测定雪旺细胞的细胞周期。结果 培养1、2d时，bFGF组的雪旺细胞计数、活力明显高于PLGA微球组和PELA微球组；培养3、4d时，bFGF组和PLGA微球组的雪旺细胞计数、活力明显高于PELA微球组；培养6、8d时．PLGA微球组的雪旺细胞计数、活力明显高于bFGF组和PELA微球组。流式细胞仪检测结果显示，培养2d后，bFGF组的Gz／M＋S期百分数最高；培养4、8d后，PLGA微球组的G2／M＋S期百分数最高，差异具有统计学意义。结论 游离bFGF对雪旺细胞促分裂增殖作用效应期短，而PLGA微球能在较长时期内促进雪旺细胞的分裂增殖，PELA微球虽然达到了缓释的性能要求，但释放出的bFGF的生物活性受到了破坏。     

Double fixation of displaced patella fractures using bioabsorbable cannulated lag screws and braided polyester suture tension bands

Purpose

To evaluate the effectiveness and safety of a new double fixation technique for displaced patellar fractures using bioabsorbable cannulated lag screws and braided polyester suture tension bands.

Methods

Fifteen patients (mean age of 46.2 years) with displaced transverse or comminuted patella fractures were enrolled in this prospective study. All of the patients were treated via the open reduction internal fixation (ORIF) procedure using bioabsorbable cannulated lag screws and braided polyester suture tension bands. The patients were followed post-surgery to evaluate (1) the time required for radiographic bone union, (2) the knee joint range of motion at the time of radiographic bone union, (3) the degree of pain assessed using the visual analogue scale (VAS), (4) the function of the knee using the Lysholm score and (5) the presence of any additional complications from the surgery.

Results

All of the patients were followed post-treatment for more than 1 year (range, 12–19 months; mean post-treatment follow up time, 14 months). The bone union of the fractures as seen radiographically occurred approximately 3 months from surgery in all cases without implant failure or redisplacement of the fractured site. The mean knee joint range of motion was from 0 to 134.6°, and the mean VAS score was 0.7 at the time of bone union. The mean Lysholm scores at the time of bone union and 12 months post-surgery were 86.7 and 95.7, respectively. No postoperative complications, such as infection, dislocation or breakage of the implants, were observed. Moreover, all of the patients returned to their previous activity level.

Conclusion

This new double fixation technique using bioabsorbable cannulated lag screws and braided polyester suture tension bands resulted in satisfactory outcomes for patella fractures without any obvious complications.     

靶向RANKL基因的siRNA对成骨细胞促破骨细胞生成作用的影响

目的利用RNA干扰(RNAi)技术抑制大鼠成骨细胞核激活因子受体配体(Ligand of receptor activator of NF-κB,RANKL)的表达,观察成骨细胞RANKL/OPG比值的变化,探讨成骨细胞与破骨细胞生成的相关性。方法合成4条RANKL序列特异性小干扰RNA(siRNA),用Liopfectamin2000转染成骨细胞,采用荧光实时定量聚合酶链反应(PCR)检测RANKL的表达,筛选出最有效的干扰序列,并用Western blot技术检测成骨细胞的RANKL、OPG的表达,观察RANKL/OPG比例的变化,采用成骨细胞破骨细胞共培养技术探讨转染后的成骨细胞对破骨细胞生成的影响。结果合成的4对siRNA中有一对可使大鼠成骨细胞的RANKL mRNA水平下降89%,蛋白表达下降76%;同时OPG的蛋白表达无明显变化,RANKL/OPG比例明显下调,破骨细胞的生成明显受到抑制。结论RNAi沉默RANKL基因表达可显著下调成骨细胞RANKL/OPG的比值,抑制破骨细胞的生成。     

骶尾部脊索瘤肢体多发性转移治疗一例报道(英文)

Chordoma is rare,locally aggressive malignant neoplasm originated from notochord remnants.Distant extrapulmonary metastases are rare and mostly occur in patient with local recurrence.In this case report,2 years after the radical resection of sacral chordoma,two times successive metastases to the upper limb were diagnosed by immunohistochemistry,without local recurrence.The metastatic tumors were radical excised,and the patient gained radiotherapy postoperatively.More than 2 years after the wide resection of the metastatic focuses,no sign of recurrence and metastasis was detected.     

股骨近端骨折内固定术后慢性感染的二期全髋关节置换

 目的 探讨二期全髋关节置换(total hip arthroplasty,THA)治疗成人股骨近端骨折内固定术后慢性感染的近期临床疗效。方法 回顾性分析2006年9月至2011年6月采用二期THA手术治疗的成人股骨近端骨折内固定术后慢性感染9例患者的临床资料,男8例,女1例;年龄31~74岁,平均52.6岁。股骨颈骨折3例,股骨转子间骨折6例。术前行关节腔穿刺液及术中取关节周围组织送细菌培养及药敏试验。一期手术取出内固定,清除感染病灶,植入抗生素骨水泥临时假体。术后静脉滴注及口服抗生素。停用抗生素后复查红细胞沉降率、C反应蛋白,间隔1个月连续两次正常后,行二期手术取出抗生素骨水泥临时假体,植入非骨水泥全髋关节假体。结果 2例细菌培养阳性,其中1例为表皮葡萄球菌、1例为金黄色葡萄球菌,其余7例细菌培养阴性。一期术后静脉应用抗生素6~8周,平均6.4周;口服抗生素6~9周,平均6.7周。一期与二期手术间隔6~14个月,平均9个月。9例患者均获得随访,随访时间6~50个月,平均23.2个月。随访期间无一例发生感染复发或新发假体周围感染,假体松动、脱位及假体周围骨溶解。Harris髋关节评分由术前平均74.6分(64~86分)提高至末次随访时平均90.9分(86~97分)。结论 二期THA手术治疗股骨近端内固定术后慢性感染,可以重建关节功能,术后近期无感染复发。     

The M1 form of tumor-associated macrophages in non-small cell lung cancer is positively associated with survival time

Background  

Tumor-associated macrophages (TAMs) play an important role in growth, progression and metastasis of tumors. In non-small cell lung cancer (NSCLC), TAMs' anti-tumor or pro-tumor role is not determined. Macrophages are polarized into M1 (with anti-tumor function) and M2 (with pro-tumor function) forms. This study was conducted to determine whether the M1 and M2 macrophage densities in NSCLC are associated with patient's survival time.     

Mitochondrial control region diversity in Sindhi ethnic group of Pakistan

The entire mitochondrial DNA control region (nt 16024–576) of 88 unrelated individuals of Sindhi ethnic group residing in different parts of Sindh province of Pakistan was sequenced. Out of 66 different observed haplotypes 50 were unique and 16 were shared by more than one individual. Results showed admixture of mtDNA pool constituting the haplogroups derived mainly from South Asia (47.6%) and West Eurasian (35.7%) whereas the contribution of the African haplogroup was very small (2.4%). High values of genetic diversity (0.992), power of discrimination (0.981) and low value of random match probability (0.018) indicates that mtDNA analysis for this population can effectively be used for forensic casework. The results are valuable contribution towards building mtDNA population variation database for this particular ethnic group from Pakistan.     

Haplotype diversity of 17 Y-chromosome STR loci in Han population from different areas of Sichuan Province,Southwest China

The distribution of 17 Y-chromosome short tandem repeat (STR) loci, included in the AmpFlSTR®Yfiler™ amplification kit, were analyzed in six different samplings (N = 878) from Sichuan, China. Haplotype diversity and discrimination capacity (DC) values were calculated. Pairwise Rst values were evaluated in AMOVA analysis and visualized through multidimensional scaling (MDS). A total of 547 unique haplotypes were detected. The observed haplotype diversity and discrimination capacity were 0.9995 and 0.7745, respectively. The homogeneity of Sichuan Han population was detected when microareas were analyzed. This population exhibited no significant genetic difference to both of the minorities in reference databases, Mongolian and Manchu, which had been through mass ethnic amalgamation with Sichuan Han population in history.