从五行学说探讨全心衰竭与脏腑的关系

心力衰竭的发生、发展及演变涉及全身多个器官,其发展演变与中医学脏腑之间生克乘侮密切相关,用中医五行学说探讨全心衰竭与脏腑之间的关系,为心力衰竭发生、发展的研究开辟一条新的道路,有利于指导心力衰竭的中医药治疗。     

从彭勃治"暗瘀"说探索肝纤维化的中医论治

系统阐述了彭勃治"暗瘀"说的基本内涵,并以此学说为基础进一步提出了"肝纤维化期相当于暗瘀期"的新观点。并解决了肝纤维化的中医病名、病因病机、辨证论治等诸多难题,从而使肝纤维化的中医论治有了自身的系统的中医基础理论的支持和准确的切入点,并有望取得突破。     

活血三步法对卵巢过度刺激征的防治及对妊娠结局的影响

目的:探讨"活血三步法"对卵巢过度刺激征(OHSS)的防治及对妊娠结局的影响。方法:对70例因多囊卵巢综合征、年轻、瘦小、有高敏感性的不孕症应用促排卵后每侧卵巢有3~5个为16mm以上卵泡生长的患者,分为治疗组与对照组。治疗组采用"活血三步法"。并采用血府逐瘀汤做对照。结果:治疗组与对照组卵泡破裂数、周期取消率、临床妊娠率,对比差别均有统计学意义(P<0.05)。与对照组对比,轻度OHSS发生率有增高趋势,但轻、中、重度OHSS发生率、流产率,多胎妊娠率、减胎率,孕12周胎儿存活率,对比差别均无统计学意义(P>0.05)。结论:"活血三步法"能有效减少中、重度OHSS的发生,提高临床妊娠率,不增加流产率及多胎妊娠率,对于促排卵中高反应病人是避免周期取消的一个良好选择。     

Penetration moxibustion for postpartum subinvolution of uterus: Two case reports and mechanism analysis: 透灸法成功治疗产后子宫复旧不良:两则病例报告及机制分析

This article introduced the application of penetration moxibustion in successfully treating two cases of postpartum subinvolution of uterus. The main complaint of one case was increasing volume of abnormal vaginal bleeding for five days after delivery. The bleeding stopped after the patient received 7 times treatment of warming needle and penetration moxibustion on Zhōngjí (中极CV3), Guānyuán (关元CV4) and Qìhăi (气海CV6). The other case complained about persistent abdominal pain for 15 days, which was worsening for the recent 2 days. The symptom of abdominal pain was relieved after the penetration moxibustion was applied on CV4, CV6 and Zǐgōng (子宫 EX-CA1) for 5 times for the case. Penetration moxibustion can relieve abdominal pain during the postpartum period, accelerate lochia discharge, and recovery of menstrual and uterine cavity. Àiyè (艾叶 Artemisiae Argyi Folium) was proved to have antibacterial, hemostatic, and analgesic effects. The warming function and radiation features of moxibustion can help to alleviate spasm of the smooth muscle in the uterus and enhance the metabolism of the uterine tissue by expanding microvessels and improving the circulations of both blood and lymph. It can also activate cells in the uterine tissue by increased temperature in order to enhance the decomposition and elimination of pathological wastes, such as inflammation and hematoma. In addition, it can also relieve pelvic pain by inhibiting neuronal excitability.     

Rasch analysis of a Spanish language-screening parent survey

The purpose of this study was to evaluate and refine items from a parent survey designed to screen the language skills of Spanish-speaking preschoolers. This investigation applied Rasch modeling to systematically evaluate and identify items that demonstrated favorable qualities.A set of 124 parent survey items was administered to 107 Spanish-speaking parents of preschool age children. Parents completed survey items intended to provide a global measure of preschool language abilities. Rasch analyses of the survey items were conducted using WINSTEPS.Results indicated that 59 items, all vocabulary items, fit the Rasch model. Sufficient unidimensionality was obtained, with the model accounting for 58% of the variance. Item difficulty estimates ranged from −7.43 to 4.12, with a shortage of items at both the lower ability level and at the higher ability level. Analyses of pruned and remaining items identified the type of items that may be most useful for a refined item bank. These results will inform the development of new items for a Spanish language-screening parent survey for preschool age children.     

The prevalence of mild cognitive impairment and its etiological subtypes in elderly Chinese

BackgroundEpidemiologic studies on mild cognitive impairment (MCI) are limited in China.MethodsUsing a multistage cluster sampling design, a total of 10,276 community residents (6096 urban, 4180 rural) aged 65 years or older were evaluated and diagnosed with normal cognition, MCI, or dementia. MCI was further categorized by imaging into MCI caused by prodromal Alzheimer's disease (MCI-A), MCI resulting from cerebrovascular disease (MCI-CVD), MCI with vascular risk factors (MCI-VRF), and MCI caused by other diseases (MCI-O).ResultsThe prevalences of overall MCI, MCI-A, MCI-CVD, MCI-VRF, and MCI-O were 20.8% (95% confidence interval [CI] = 20.0–21.6%), 6.1% (95% CI = 5.7–6.6%), 3.8% (95% CI = 3.4–4.2%), 4.9% (95% CI = 4.5–5.4%), and 5.9% (95% CI = 5.5–6.4%) respectively. The rural population had a higher prevalence of overall MCI (23.4% vs 16.8%, P < .001).ConclusionsThe prevalence of MCI in elderly Chinese is higher in rural than in urban areas. Vascular-related MCI (MCI-CVD and MCI-VRF) was most common.     

桔皮素对人非小细胞肺癌细胞生长及侵袭的影响

目的:探讨桔皮素(TGN)对非小细胞肺癌(NSCLC)细胞生长和侵袭的影响及其分子机制。方法:体外培养非小细胞肺癌A549细胞,分别用不同浓度的TGN处理,MTT比色法检测细胞活性,Annexin V-FITC/PI染色及流式细胞术检测细胞凋亡率,Transwell检测细胞侵袭,RT-PCR分析MMP-2和MMP-9的mRNA表达水平,Western blotting检测Ki67、Cyt C、caspase-3、cleaved caspase-3、MMP-2、MMP-9、Akt、p-Akt以及p-PI3K表达水平。结果:桔皮素剂量依赖性地抑制A549细胞增殖(P0.05),同时伴随有增殖标记分子Ki67表达水平的下调。分析发现,桔皮素诱导细胞中Cyt C、caspase-3和cleaved caspase-3的表达上调(P0.01),加速A549细胞的凋亡。此外,桔皮素作用后,A549细胞中侵袭相关蛋白MMP-2和MMP-9的表达量下降,且侵袭数目随桔皮素浓度增加而减少。进一步研究表明,桔皮素作用后A549细胞中p-Akt和p-PI3K表达水平降低(P0.05),阻断PI3K/Akt信号通路后,不同浓度TGN对细胞活性影响没有变化。结论:桔皮素能抑制A549细胞生长及侵袭,促进细胞凋亡,可能通过抑制PI3K/Akt信号通路的激活起作用。因此,本研究将为非小细胞肺癌的防治提供新的研究方向。     

糖尿病微血管病变发生机制研究现状

糖尿病微血管病变是糖尿病慢性并发症的病理基础,病理机制涉及内皮受损及一氧化氮合成减少、多元醇代谢异常、糖化反应的亢进、氧化应激、蛋白激酶C激活等多个方面,现对糖尿病微血管病变的研究现状进行探讨,以提高对糖尿病微血管病变的认识水平。     

Panleucogating方法计数CD4^＋T淋巴细胞的研究进展

摘要：目的阐述Panleucogating方法计数艾滋病病毒（HIV）感染者／AIDS病人（HIV／AIDS病人）CD4^＋T淋巴细胞（CO4细胞）的方法、发展和应用。方法收集HIV／AIDs病人CD4^＋细胞检测方法的文献,分析Panleucogating方法的可行性及其优势和不足。结果发现Panleucogating方法与常规方法检测的CD4^＋细胞数值之间存在较高的相关性。该方法具有检测成本低、操作简单、检测迅速等优势。结论Panleucogating方法可用于HIV／AIDS病人CD4细胞计数的检测,在经济欠发达地区有应用价值。