Vaxvec: The first web-based recombinant vaccine vector database and its data analysis

A recombinant vector vaccine uses an attenuated virus, bacterium, or parasite as the carrier to express a heterologous antigen(s). Many recombinant vaccine vectors and related vaccines have been developed and extensively investigated. To compare and better understand recombinant vectors and vaccines, we have generated Vaxvec (http://www.violinet.org/vaxvec), the first web-based database that stores various recombinant vaccine vectors and those experimentally verified vaccines that use these vectors. Vaxvec has now included 59 vaccine vectors that have been used in 196 recombinant vector vaccines against 66 pathogens and cancers. These vectors are classified to 41 viral vectors, 15 bacterial vectors, 1 parasitic vector, and 1 fungal vector. The most commonly used viral vaccine vectors are double-stranded DNA viruses, including herpesviruses, adenoviruses, and poxviruses. For example, Vaxvec includes 63 poxvirus-based recombinant vaccines for over 20 pathogens and cancers. Vaxvec collects 30 recombinant vector influenza vaccines that use 17 recombinant vectors and were experimentally tested in 7 animal models. In addition, over 60 protective antigens used in recombinant vector vaccines are annotated and analyzed. User-friendly web-interfaces are available for querying various data in Vaxvec. To support data exchange, the information of vaccine vectors, vaccines, and related information is stored in the Vaccine Ontology (VO). Vaxvec is a timely and vital source of vaccine vector database and facilitates efficient vaccine vector research and development.     

绦虫基因组学研究进展

绦虫是一类严重危害人类身体健康的寄生虫,每年给人类造成很大的经济负担。绦虫种类众多,生物学特性各异,目前绦虫病的防控和治疗措施尚不完善,对动物和人类的危害严重。随着生物信息学和基因测序技术的发展、绦虫基因组序列的测定和解析,通过对绦虫的生物学特征和调控基因进行深度研究有助于预防和控制绦虫病,特别是人和动物的棘球蚴病和囊虫病等重要的人兽共患寄生虫病。利用绦虫基因组学和蛋白质组学等组学技术,筛选绦虫感染关键的基因和蛋白质,提供绦虫病的诊断参考依据和绦虫病治疗的潜在药物靶标。本文就绦虫基因组测序完成情况和近年来绦虫基因组学研究进展进行综述。     

磨积散颗粒冲剂联合FLO方案治疗晚期胃癌临床随机对照研究

[目的]探讨晚期胃癌有效治疗途径.[方法]晚期胃癌49例,对照组23例采用选择常用的FLO方案联合化疗.治疗组24例采用选择常用的FLO方案联合化疗同时加用磨积散颗粒冲剂.[结果]近期疗效治疗组有效率54.17%;对照组好转率47.83%.arnofsky评分比较,治疗组Karnofsky评分好转率62.50%,对照组为52.17%(P<0.05).体力、体重、食欲和癌痛变化变化情况,治疗组好转率、稳定率、进展率均好于对照组(P<0.05).血细胞学主要指标,治疗后两组患者血细胞学主要指标均有不同程度改变,其中WBC、Hb下降明显,尤其在治疗后3月后,单纯化疗的患者各项指标的下降更为显著,两组积分比较(P<0.05).[结论]磨积散颗粒剂能够提高化疗疗效,降低化疗毒副作用,提高生活质量、延长生存期.     

系统生物学促进中医现代化

系统生物学的系统论与整体论的观点,与中医的整体观念、辨证论治的基本特点不谋而合,系统生物学的研究与发展为中医现代化开辟了新的方向。系统生物学的研究理念为中医研究的现代化提供了新的思路和启示,使传统中医与现代科技研究接轨、走向国际赋予了新的可能。中医将会绽放出新的活力,更好地服务人类的健康事业。     

甲型H1N1流感病毒HA基因的原核表达及免疫反应性分析

目的构建甲型H1N1流感病毒HA基因的原核表达质粒,获得融合表达蛋白,并对表达蛋白的免疫反应性进行分析。方法人工合成A/California/05/2009 H1N1流感病毒的HA基因,以合成基因为模板,通过PCR方法扩增出去除信号肽的HA部分基因片段,然后将去除信号肽的HA基因克隆至pET30a(+)原核表达载体中,构建出原核表达质粒,再将重组质粒转化E.coliBL21(DE3)表达菌株;重组菌经IPTG诱导后,收集菌体进行SDS-PAGE电泳分析,Western blot分析表达产物的免疫反应性。结果获得了HA基因的原核表达重组菌,细菌经IPTG诱导表达后,SDS-PAGE分析可见约67.4 ku大小的目的蛋白表达条带,Western blot结果显示,表达产物与人甲型H1N1流感患者阳性血清具有反应性。结论成功表达出甲型H1N1流感病毒的HA蛋白,该蛋白具有良好的免疫反应性,为甲型H1N1流感的快速诊断方法的建立提供了生物材料。     

聚乙烯亚胺介导NIH-3T3细胞体外转染pEGFP-N1系统优化

目的 优化聚乙烯亚胺(polyethylenimine,PEI)介导的细胞转染以提高目的 基因在细胞中的表达强度.方法 根据PEI/DNA不同的质量比(0:1、0.5:1、1:1、2:1、3:1、4:1、5:1、6:1、7:1、8:1、9:1、10:1)利用凝胶阻滞分析结合的情况;不同质量比PEI/DNA形成的复合物对NIH-3T3细胞的转染通过细胞表达强度获得最佳PEb/DNA质量比;比较实验组l(PEI/DNA质量比2:1)、实验组2(重复PEL/DNA质量比2:1)及实验组3(PEI/DNA质茸比4:2)3种转染方案,探讨重复转染的方法是否提高细胞表达强度.结果 ①通过凝胶阻滞分析PEI/DNA能够稳定结合的质量比是1:1～10:1;②PEI/DNA复合物对NIH-3T3细胞进行转染,其荧光表达强度当PEI/DNA(质量比)≤2时随着PEI的增加而提高,当PEI/DNA(质量比)>2时,荧光表达强度反而降低;③采用重复转染的方法实验组2细胞荧光表达强度(24.08±0.28)%明显高于实验组1(8.97±4.02)%和实验组3(14.24±2.68)%(P<0.05).结论 在PEI/DNA(质量比)=2的条件下,PEI/DNA复合物转化NIH-3T3细胞的效果比较理想,重复转染可以提高细胞转染后荧光表达强度.     

高锰酸钾滴定液(0.02mol·L~(-1))浓度标定的不确定度评定

目的对按《中国药典》2010年版方法配制和标定的高锰酸钾滴定液(0.02mol.L-1)浓度进行不确定度评定。方法建立数学模型,对标定过程中各种影响因素进行分析评估。结果通过计算各变量的不确定度分量,计算出合成不确定度。代入包含因子(k),得到本次标定的扩展不确定度。结论建立的标定高锰酸钾滴定液浓度的不确定度评定方法合理、可行。     

黄藤素片体外溶出度考察

目的:测定5个厂家黄藤素片的溶出度,考察产品质量。方法:采用紫外分光光度法,以盐酸溶液(0.1mol·L-1)900mL为溶出介质,测定黄藤素片主成分盐酸巴马汀的溶出量。结果:5个厂家黄藤素片的溶出参数T30、T50、T80、m、Kr有显著差异(P<0.05)。结论:产品质量与生产工艺密切相关。     

A metagenomic examination of the pathobiome of the invasive tick species,Haemaphysalis longicornis,collected from a New York City borough,USA

Haemaphysalis longicornis, the Asian longhorned tick, is an invasive tick species that has spread rapidly across the northeastern and southeastern regions of the United States in recent years. This invasive pest species, known to transmit several tick-borne pathogens in its native range, is a potential threat to wildlife, livestock, domestic animals, and humans. Questing larval (n = 25), nymph (n = 10), and adult (n = 123), along with host-derived adult (n = 25) H. longicornis ticks were collected from various locations on Staten Island, NY. The pathobiome of each specimen was examined using two different high throughput sequencing approaches, virus enrichment and shotgun metagenomics. An average of 45,828,061 total reads per sample were recovered from the virus enriched samples and an average of 11,381,144 total reads per sample were obtained using shotgun metagenomics. Aside from endogenous viral sequences, no viruses were identified through either approach. Through shotgun metagenomics, Coxiella-like bacteria, Legionella, Sphingomonas, and other bacterial species were recovered. The Coxiella-like agent was ubiquitous and present at high abundances in all samples, suggesting it may be an endosymbiont. The other bacterial agents are not known to be transmitted by ticks. From these analyses, H. longicornis do not appear to host any endemic human tick-borne pathogens in the New York City region.     

小儿青翘颗粒对呼吸道病原菌的体外抑制作用研究

目的:了解小儿青翘颗粒体外抗呼吸道病原菌的活性。方法:采用微量法测定小儿青翘颗粒对临床分离的肺炎链球菌、鲍曼不动杆菌、肺炎克雷伯菌和副流感嗜血杆菌的最低抑菌浓度(MIC)和最小杀菌浓度(MBC);采用平板法测定小儿青翘颗粒对鲍曼不动杆菌的杀菌时效与量效曲线。结果:小儿青翘颗粒对大部分呼吸道病原菌均有较好抗菌效果,对肺炎链球菌、鲍曼不动杆菌、肺炎克雷伯菌和副流感嗜血杆菌的MIC范围分别为9.38~75、4.69~9.38、1.17~4.69和4.69~75 mg/mL;MBC范围分别为18.75~75、4.69~9.38、2.34~4.69和4.69~75 mg/mL。结论:小儿青翘颗粒对常见呼吸道病原菌均具有较好的抑菌作用,对鲍曼不动杆菌的抑菌效果最好。