CGRP对多梗死大鼠海马CA4区NPY细胞影响的研究

目的探讨CGRP对MCI的影响。方法选用Wistar大鼠，应用微栓子栓塞阻断法建立多发性脑梗死性缺血模型，借用ABC免疫组织化学染色方法、透射电镜技术并结合显微图像分析观察大鼠海马CA4区神经元的NPY变化。结果治疗组NPY阳性反应物平均灰度值与MCI组比较低（P〈0．05）。结论CGRP对MCI的恢复有具有一定的关系。     

微创下中空加压螺钉内固定治疗锁骨骨折

目的探讨微创手术治疗锁骨中段骨折的方法及效果。方法对23例锁骨骨折采用微创下中空加压螺钉内固定治疗。结果所有患者均获随访,随访时间5～16个月(平均6.7个月)。X线示骨折愈合,愈合时间6～8周(平均6.6周),无畸形或延期愈合及并发症发生,外观良好,患者满意。结论微创下中空加压螺钉内固定治疗锁骨骨折是一种损伤小、操作简单、疗效优良的手术方法。     

高聚金葡素对骨折愈合的影响

目的：研究高聚金葡素促骨折愈合的作用。方法：通过大鼠闭合错位型骨折及大鼠手术缺损型模型研究其康复作用，并与麝香接骨丹进行比较。结果：高聚金葡素骨折断端注射，对大鼠闭合错位型骨折能促进骨痂形成，加速骨折愈合。结论：高聚金葡素康复效果明显优于麝香接骨丹。     

FADD-DN基因转梁对氦氖激光诱导瘢痕成纤维细胞凋亡的影响

目的探讨氦氖激光诱导瘢痕成纤维细胞凋亡的分子机制。方法将培养瘢痕成纤维细胞随机分为转染组、转染对照组和非转染组,转染组细胞与绿色荧光蛋白(GFP)质粒cDNA、含目的基因片段的表达载体质粒pcDNA3-FADD-DNcDNA共转染,转染对照组细胞与GFP质粒cD-NA、空载体质粒pcDNA3共转染,非转染组细胞没有进行转染,转染后24h分别对各组细胞进行氦氖激光照射(100mW/cm2照射30min),1次/d,连续照射3d,然后采用TUNEL技术检测细胞凋亡。结果转染组、转染对照组与非转染组细胞凋亡率分别为9.27%,15.73%和12.87%,转染组细胞凋亡率明显小于转染对照组(q=4.55,P<0.01)和非转染组(q=4.14,P<0.05)。结论FADD-DN基因转染能减少氦氖激光诱导的瘢痕成纤维细胞凋亡,FADD可能是氦氖激光激活死亡信号途径的基本元件之一。     

Distribution and drug resistance of pathogens in burn patients in China from 2006 to 2019

BACKGROUNDIn this study, recent trends in the distribution and drug resistance of pathogenic bacteria isolated from patients treated at a burn ward between 2006 and 2019 were investigated.AIMTo develop more effective clinical strategies and techniques for the prevention and treatment of bacterial infections in burn patients.METHODSClinical samples with positive bacteria were collected from patients at the burn ward in Beijing Jishuitan Hospital in China between January 2006 and December 2019. The samples were retrospectively analyzed, the distribution of pathogenic bacteria was determined, and the trends and changes in bacterial drug resistance during different period were assessed. Drug resistance in several main pathogenic bacteria from 2006 to 2011 and from 2012 to 2019 was comparatively summarized and analyzed.RESULTSSamples from 17119 patients were collected and analyzed from 2006 to 2019. Surprisingly, a total of 7960 strains of different pathogenic bacteria were isolated at this hospital. Among these bacteria, 87.98% (7003/7960) of the strains were isolated from burn wounds, and only 1.34% (107/7960) were isolated from the blood of patients. In addition, 49.70% (3956/7960) were identified as Gram-positive bacteria, 48.13% (3831/7960) were Gram-negative bacteria, and the remaining 2.17% (173/7960) were classified as fungi or other pathogens. Importantly, Staphylococcus aureus (21.68%), Pseudomonas aeruginosa (14.23%), and Staphylococcus epidermidis (9.61%) were the top three pathogens most frequently isolated from patients.CONCLUSIONIn patients treated at the burn ward in this hospital from 2006 to 2019, Staphylococcus aureus and Pseudomonas aeruginosa were the predominant clinical pathogens responsible for bacterial infections. The circumstantial detection and detailed monitoring of the intensity and growth of different pathogenic bacteria in clinical patients as well as tests of drug sensitivity during burn recovery are particularly important to provide guidelines for the application of antibiotics and other related drugs. Careful collection and correct, standard culture of bacterial specimens are also crucial to improve the efficiency of bacterial infection detection. Effective monitoring and timely clinical treatment in patients may help reduce the possibility and rate of infection as well as alleviate the effects of drug resistance among patients in burn centers.     

宫腔镜联合B超检查在诊断异常子宫出血中的作用及临床观察

目的：探讨宫腔镜联合B超检查在诊断异常子宫出血中的作用。方法：选择2000年5月－2000年12月我院176例经宫腔镜联合B超检查并行组织病理诊断的异常子宫出血患者，分析宫腔镜联合B超检查与病理诊断的符合情况。结果：176例宫腔镜联合B超检查与病理诊断的总符合率为83.00%，与初诊总符合率（29.50%）及单纯B超检查总符合率（59.10%）比较均有显著性差异（P＝0.000）。176例宫腔镜联合B超检查中子宫粘膜下肌瘤（3／3）、宫颈肌瘤（3／3）、宫颈息肉（7／7）、宫腔内胚胎残留（13／13）病理诊断的符合率均为100.00%，子宫内膜息肉为95.60%（43／45）、慢性子宫内膜炎为86.00%（43／50）、子宫内膜增生过长为63.04%（29/46）、子宫内膜癌为50.00%（1／2）。结论：宫腔镜联合B超检查可明显提高异常子宫出血症状诊断的准确性，但不能取代组织病理学诊断。     

从高血压到脑卒中的脑血管血流动力学指标变化规律

目的:探讨从高血压到脑卒中的脑血管血流动力学指标(cerebralvascu-larhemodynmic,CVHD)的变化规律。方法:队列人群中随访发生的371例有高血压病史的脑卒中患者按年龄、性别随机选择其它组别,分成血压正常组、临界高血压组、高血压组、脑卒中前高血压组和脑卒中后遗症组。分析和观察CVHD的分布和变化规律。结果:5组人群的CVHD积分中位数分别为95.5,90.0,78.75,40.0和51.25分,其中<75分的百分率分别为19.9%,36.8%,46.3%,78.7%和69.5%;从血压正常到脑卒中发病前,脑血管血流速度呈明显的降低趋势,外周阻力和特性阻抗等动力学指标呈明显的升高趋势。结论:随着高血压的发生和发展,脑血管功能异常逐渐加重,当总积分值下降至40分左右,为脑卒中发病前的“超高危”状态,是脑卒中发生的重要预警信号。     

HPLC法测定血浆中法罗培南浓度的不确定度分析

目的:对HPLC法测定血浆中法罗培南浓度的测量不确定度进行分析,以找到影响不确定度的因素,为评价检测报告提供科学依据。方法:根据《测量不确定度评定与表示》,并参考《化学分析中不确定度的评估指南》,对血浆中法罗培南含量测定进行测量不确定度的分析与评定。结果:标准不确定度为1.28μg·ml^-1,扩展不确定度为2.56μg·ml^-1,含量测定结果可表示为（10.14±2.56）μg·ml^-1。结论:建立的不确定度评估方法可用于HPLC法测定血浆中法罗培南含量的不确定度评估。测量不确定度的评定方法的确立对于血浆中法罗培南含量测定方法标准的研究具有重要意义。     

免疫亲和柱净化光化学衍生高效液相色谱-荧光检测法测定动物类药材中黄曲霉毒素

目的建立动物类药材中黄曲霉毒素B1,B2,G1,G2的免疫亲和柱净化光化学衍生高效液相色谱-荧光检测(HPLC-FLD)法。方法样品经甲醇-水(80∶20)提取后,通过免疫亲和柱净化、柱后光化学衍生、高效液相色谱-荧光检测器测定。结果在优化条件下,黄曲霉毒素G2,G1,B2,B1的检出限分别为0.15,0.25,0.1,0.2μg/kg,回收率为78.8%～106.7%,RSD均低于7.1%。结论所用方法简便快速、灵敏度高、重现性好,可满足动物类药材中黄曲霉毒素检测的需要。     

腹水超滤浓缩回输联合静脉输入人血白蛋白、利尿治疗顽固性腹水疗效观察

目的:评估腹水超滤浓缩回输联合输入人血白蛋白、利尿治疗顽固性腹水的临床疗效.方法:将我院2006～2010年收治的214例乙肝肝硬化腹水病例经基础治疗3～4天效果不好的78例,随机分为三组,第一组采用传统纠正低蛋白血症、利尿内科治疗;第二组采用单纯性腹水超滤浓缩回输治疗;第三组采用腹水超滤浓缩回输联合输入人血白蛋白、利...