大肠埃希菌脂多糖紫外光谱检出方法的研究

目的 通过对大肠埃希菌、肺炎克雷伯菌、铜绿假单胞菌等细菌脂多糖的紫外光谱分析,探讨用259 nm波长处紫外可见光光谱分析大肠埃希菌脂多糖的方法.方法 酶解法提取临床分离的TH1、TH2、TH6、TH7、P7、K11脂多糖;鲎变形细胞溶解物试验测定其体外内毒素活性,紫外可见光光谱扫描测定其吸光度,凝胶色谱法纯化,硫酸-苯...     

议王好古论治阴证之特点

王好古,元代医家,将伤寒学说与脾胃内伤学说有机地结合而创立阴证学说。认为阴证的病因为外感寒、内饮冷,＂人本气虚＂和＂内已伏阴＂;病机为＂元阳中脱＂;辨证着重三阴阳虚证;治疗亦偏重于温补,善用附子、干姜,并讲究服药时间、方法。其对于阴证的论治,既补充了仲景之学,又发挥了易水学说,至今仍具有较大的实用价值。     

Efficacy and Safety of Rezivertinib (BPI-7711) in Patients With Locally Advanced or Metastatic/Recurrent EGFR T790M-Mutated NSCLC: A Phase 2b Study

IntroductionRezivertinib (BPI-7711) is a novel third-generation EGFR tyrosine kinase inhibitor (TKI) targeting both EGFR-sensitizing mutations and EGFR T790M mutation. This study aimed to evaluate the efficacy and safety of rezivertinib in patients with locally advanced or metastatic/recurrent EGFR T790M-mutated NSCLC.MethodsPatients with locally advanced or metastatic/recurrent NSCLC with confirmed EGFR T790M mutation who progressed after first-/second-generation EGFR TKI therapy or primary EGFR T790M mutation were enrolled. Patients received rezivertinib at 180 mg orally once daily until disease progression, unacceptable toxicity, or withdrawal of consent. The primary end point was objective response rate (ORR) assessed by blinded independent central review per Response Evaluation Criteria in Solid Tumors version 1.1. Secondary end points included disease control rate (DCR), duration of response, progression-free survival (PFS), overall survival, and safety. This study is registered with Clinical Trials.gov (NCT03812809).ResultsA total of 226 patients were enrolled from July 5, 2019, to January 22, 2020. By the data cutoff date on January 24, 2022, the median duration of follow-up was 23.3 months (95% confidence interval [CI]: 22.8–24.0). The ORR by blinded independent central review was 64.6% (95% CI: 58.0%–70.8%), and DCR was 89.8% (95% CI: 85.1%–93.4%). The median duration of response was 12.5 months (95% CI: 10.0–13.9), and median PFS was 12.2 months (95% CI: 9.6–13.9). The median overall survival was 23.9 months (95% CI: 20.0–not calculated [NC]). Among 91 (40.3%) patients with central nervous system (CNS) metastases, the median CNS PFS was 16.6 months (95% CI: 11.1–NC). In 29 patients with more than or equal to one brain target lesion at baseline, the CNS ORR and CNS DCR were 69.0% (95% CI: 49.2%–84.7%) and 100% (95% CI: 88.1%–100%), respectively. Time to progression of CNS was 16.5 months (95% CI: 9.7–NC). Of 226 patients, 188 (83.2%) had at least one treatment-related adverse event, whereas grade more than or equal to 3 occurred in 45 (19.9%) patients. No interstitial lung disease was reported.ConclusionsRezivertinib was found to have promising efficacy and favorable safety profile for patients with locally advanced or metastatic/recurrent NSCLC with EGFR T790M mutation.     

5-Aza-CdR对CEM细胞生长抑制的实验研究

目的:观察不同剂量5-氮杂-2 '-脱氧胞苷(5-Aza-2’-deoxycytidine,5-Aza-CdR)在体外作用48 h 对人急性淋巴细胞白血病CEM细胞生长的影响.方法:用MTS法及流式细胞仪分析5-Aza-CdR对CEM细胞的生长抑制情况.结果:1.0、2.5、5.0μmol/L的5-Aza-CdR处理CEM细胞48 h,对CEM细胞的抑制率分别为1.6％、5.6％、7.5％,5.0 μmol/L有明显抑制作用(P＜ 0.01); 5-Aza-CdR可使CEM细胞凋亡率增加,且作用呈浓度依赖性,1.0、2.5、5.0μmol/L组细诱导胞早期凋亡率分别为4.8％、8.2％、13.8％.结论:5-Aza-CdR能抑制CEM细胞生长,并且可诱导其凋亡.     

Prolift盆底重建术35例临床分析

目的 探讨Prolift盆底重建术对盆腔器官脱垂的治疗效果.方法 回顾性分析2010-02~2010-08我院使用Prolift盆腔修复系统治疗不同程度、不同类型的盆腔器官脱垂患者35例的临床疗效、围手术期并发症.结果 应用全盆修复系统33例,前盆修复系统2例;应用全盆修复系统的病例中有2例同时行经闭孔尿道中段无张力悬吊术(TVT-O术式),5例患者行全子宫切除术后应用盆底修复系统.通过手术前后POP-Q评分系统分析疗效,痊愈30例,好转4例,复发1例.术中发生膀胱损伤者3例.结论 Prolift全盆修复系统器械设计较合理、易于被临床医师较好掌握、微创、复发率低,是治疗盆腔器官脱垂的较好选择.     

霍乱弧菌HutX蛋白的晶体结构

目的 获取纯化的霍乱弧菌HutX蛋白晶体和衍射数据.方法 通过基因克隆和蛋白表达得到HutX蛋白,通过镍柱亲和层析、Source Q阴离子交换柱层析和Superdex 200分子筛柱层析等纯化所得蛋白,并用蛋白质印迹法进行验证.将所获蛋白进行结晶条件筛选及悬滴优化,X线衍射分析所得晶体的结构.结果 蛋白质印迹分析间接证明所得到的蛋白为HutX蛋白,并得到HutX蛋白的晶体及衍射数据.结论 为进一步研究HutX的蛋白结构及其在霍乱弧菌亚铁血红素利用机制中发挥的作用提供了参考.     

镇痛泵应用于带状疱疹神经痛的疗效观察及护理

目的观察硬膜外镇痛泵在带状疱疹神经痛中的镇痛效果及护理。方法将符合纳入标准的48例病人随机分为实验组和对照组,对照组按照常规方法进行镇痛,实验组应用镇痛泵镇痛,比较两组病人的镇痛效果。结果实验组的镇痛效果明显优于对照组(P<0.05),护理效果佳。结论在带状疱疹神经痛采用硬膜外镇痛泵具有较好的止痛,但因药物作用、穿刺创伤等原因会出现并发症须加强护理,避免或减少并发症发生。     

补肾活血法治疗慢性心力衰竭探析

根据老年人肾虚、多瘀的生理病理特点和慢性心力衰竭的临床表现及现代研究,认为肾虚血瘀是慢性心力衰竭的重要病机。肾虚为本,血瘀为标,是慢性心力衰竭发生发展的主要致病因素。补肾活血是慢性心力衰竭的主要治法。     

基于光谱成像技术的红花快速鉴别与质量评价

目的 :快速鉴别红花药材的真伪,并对不同市售来源的红花样品进行质量评价,以探讨光谱成像技术在中药真伪鉴别和质量控制方面的应用与优势。方法 :应用凝视式荧光光谱成像装置,提取红花、西红花和菊花对照药材的归一化特征荧光光谱曲线,对红花药材进行鉴别;通过提取不同红花样品的归一化特征荧光光谱曲线,对不同市售来源的红花质量进行评价;系统的光谱覆盖范围为400~680 nm,光谱分辨率为5 nm。结果 :红花的特征光谱曲线与菊花、西红花显著不同;质量不同的红花样品荧光强度不同。结论 :光谱成像法可用于红花药材的真伪鉴别与质量评价,并具有操作简便、快速、无损等优点。     

急诊床旁鼻胆管引流术在急性重症胆管炎的临床应用

目的探讨急诊床旁鼻胆管引流术对急性重症胆管炎的治疗价值和实用性。方法对219例急性重症胆管炎患者实施急诊床旁非X线监视下的鼻胆管引流术。手术在入院2～6小时(平均3.4小时)内完成;以导丝顺利进入并注射器回抽出混浊的脓性胆汁或液体为插管成功的标准。结果 219例患者中,211例症状逐渐缓解,有效率为96.35%;8例因存在肝内胆管梗阻而感染控制不佳,于鼻胆管引流后1～3天加行PTCD;未发生与内镜操作有关的严重并发症,如消化道穿孔、出血、胰腺炎和死亡。结论急诊床旁非X线监视下的鼻胆管引流术对重症胆管炎早期急诊救治具有重要的临床应用价值。