挫伤性屈光不正的眼超声生物学检测

对１１２例挫伤性屈光不正患者进行了超声生物学检测。结果提示，挫伤性近视患者眼球解剖结构存在两大变，即晶体前移和厚度增加，超声前房深度、晶本厚度在伤后的检测结果与对照组相比具显著性差异。并对挫伤屈光不正的发生机制进行了探讨。     

螺旋CT对胃癌Lauren''s分型诊断价值的研究

目的探讨螺旋CT(SCT)对胃癌Lauren's分型的诊断价值. 资料与方法对40例进展期胃癌患者行SCT平扫及三期动态增强扫描.40例均于检查后1周内施行全胃或部分胃切除手术、剖腹探查术.术后标本行常规石蜡包埋及5μm切片. 结果胃壁低密度层增厚程度在肠型胃癌和弥漫型胃癌分别为1.55mm±0.55mm和7.71mm±2.33mm;强化程度在肠型胃癌和弥漫型胃癌分别为51.98HU±9.35HU和87.95HU±18.79HU,两组比较,差异均具有统计学意义(P＜0.05,P＜0.05). 结论 SCT对胃癌Lauren's分型有一定价值.     

Effect of autocontrol micromotion intramedullary interlocking nail on fracture healing： an experimental study

Objective: To investigate the effect of autocontrol micromotion locking nail ( AMLN ) on experimental fracture healing and its mechanism. Methods: 16 goats undergoing both sides of transverse osteotomy of the femoral shafts were fixed intramedullary with AMLN and Gross-Kempf (GK) nail, respectively. The follow-up time was 7, 14, 28 and 56 days. Roentgenographic, biomechanical, histological, scanning electromicroscopic and biochemical analyses were done. Results: (1) The strength of anticompression, antiflexion and antitorsion in the fractural end in the AMLN-fixed group was higher than that of GK nail-fixed group; whereas, the rate of stress shelter in the fractured end decreased significantly (P<0.01). (2) The content of the total collagen, insoluble collagen, calcium and phosphate in the AMLN-fixed group was higher than that in the GK nail-fixed group (P<0.05). (3) Histological observation and quantitative analysis of calluses revealed that AMLN could promote the growth of bridge calluses and periosteum calluses. Hence the facture healing and remolding process achieved early, which was much better than traditional GK nail fixation. (P<0.05). (4) 7-14 days postoperation, the calluses of AMLN-fixed group was flourish and camellarly arranged and the collagen fibril formed constantly in the absorption lacuna of bone trabecula. 28-56 days postoperation, the collagen fibril was flourish around the absorption lacuna and was parallel to the bone's longitudinal axis. Active bony absorption and formation were seen, so was remolding and rebuilding. Haversian system was intact and the bony structural net was very tenacious because of the deposition of calcium salt. None of the above findings was observed in the GK nailfixed group. Conclusions: The design of AMLN accords well with the plastic fixation theory. As the geometry ametabolic system constituted by the intramedullary fixation instruments and the proximal and distal end of the fracture is very firm and stable, the disturbance to the physical stress distributed in the fractural end is light. The generation and conduct of the intermittent physical stress between the fractural parts could reach the balance between stress conduct and stress protection. The feature that the healing and remolding take place at the same time speeds up the fractural healing process.     

带血管蒂岛状筋膜瓣移植治疗晚期类风湿性髋关节炎

目的：阐明带血管蒂岛状筋膜瓣移植治疗晚期类风湿性髋关节炎的疗效。材料和方法：对４２例４８髋晚期类风湿性髋关节炎病人采用以旋股外侧血管降支为蒂的股前外侧筋膜瓣移植行患髋成形术。结果：术后随访４～１０．５年，平均４．５年，髋关节活动范围术前平均１６．５°，术后２２８．６°，按照Ｃｈａｒｎｌｅｙ和吴之康人工髋关节置换术后的疗效评定标准，优良２９髋、很好１３髋、好６髋，尚可、差、很差无。结论：患者年龄在１５～４０岁之间为合适的治疗年龄。与人工髋关节置换术相比，本方法对髋关节正常结构破坏小；一旦手术失败仍可选用其它治疗方法弥补；且并发症少、费用低、远期效果好。     

脾脏切除后暴发性感染6例临床分析

目的探讨脾切除术后暴发性感染的临床的特点。方法对6例OPSI患者的原发疾病、临床特点及其病原学进行分析。结果4例原发疾病属于造血系统疾病,1例脾功能亢进,1例外伤性脾破裂;所有病例均起病急促而凶猛、病情迅速恶化,于短期内出现休克及DIC;所有病例血细菌培养均阳性,3例为G 球菌,2例为G-杆菌,1例为G-杆菌合并霉菌感染;经强有力地抗感染治疗5例痊愈,1例死亡。结论OPSI病情凶险,死亡率高,尤其是血液病患者OPSI发生率较高,医生在行脾脏切除时应慎重。     

Kringle 5的真核植物细胞穿梭表达载体的构建

目的：构建含有目的基因kring5的真核植物细胞穿酸表达载体。方法：应用RT－PCR方法，从正常人肝脏组织中获得kringle5基因，测序后，再通过DNA重组技术，将kringle5基因片段克隆到真核植物细胞表达载体pBI121和pCAMBIA3301上。结果：得到的kringle5基因序列测定结果与文献相符，重组载体pB1k5和pC33k5经酶切鉴定正确，含有植物高效表达CaMV35S启动子。结论：重组载体pB1k5和pC33k5不仅可以在大肠杆菌中稳定复制，而且可以在植物细胞中表达。     

颅内血肿旋吸多导向清除装置

本装置提供一种对脑组织损伤轻清除脑血肿速度快的微创器械,以便更加有效地清除高血压脑出血后形成的脑血肿,减轻脑出血引起的脑损害,减少死亡;该装置由外套管、旋吸管、导向管和喷射管组成,用该装置可以对脑血肿实施旋转碎吸和多导向喷射冲洗;临床用该装置治疗400余例高血压脑出血病人,迅速减轻脑血肿占位效应,使大部分病人生命得到挽救,减少了后遗症,改善了生存质量;其疗效表明该装置是目前最为有效地脑血肿微创清除器械.     

酒精对骨髓基质细胞成脂与成骨分化的影响

目的观察酒精对小鼠骨髓基质细胞中脂肪细胞特异性基因422(aP2)和Ⅰ型胶原基因表达的影响。方法采用原代骨髓基质细胞体外培养技术,取小鼠双侧股骨骨髓细胞,通过细胞贴壁、分离,获取骨髓基质细胞。以0.09mol/L的酒精浓度作为诱导剂处理细胞。采用完整细胞斑点印迹分子杂交方法检测实验组和对照组细胞中422(aP2)mRNA和Ⅰ型胶原mRNA的表达。结果对实验组和对照组的422(aP2)mRNA的完整细胞斑点印迹扫描值进行比较,实验组中422(aP2)基因杂交信号明显增强,斑点印迹扫描值为7207.8±331.3,对照组斑点印迹扫描值为652.2±62.6,实验组为对照组的11倍,两组比较差异有非常显著性意义(P<0.001)。对实验组和对照组Ⅰ型胶原mRNA的完整细胞斑点印迹扫描值进行比较,实验组基因杂交信号较对照组明显减弱,斑点印迹扫描值为3 567.3±300.9,对照组斑点印迹扫描值为7487.0±488.4,为实验组的2倍,两组比较差异有非常显著性意义(P<0.001)。结论酒精能诱导骨髓基质细胞向脂肪细胞分化,而减少骨髓基质细胞向成骨细胞分化。这可能与酒精性骨坏死的发生机制有关。     

实验性精索静脉曲张大鼠血清及睾内睾酮的变化

目的:研究精索静脉曲张(VC)大鼠模型对血清T和睾丸内T的影响。方法:通过部分结扎左肾静脉建立SD大鼠VC模型20只;假手术组SD大鼠20只为对照组。模型建立后4、8周取静脉血,并取部分睾丸组织匀浆提取上清液,放免法测定血清及睾丸内T浓度。结果:血清T:实验组4、8周与同期对照组相比有下降趋势,但没有统计学意义。双侧睾丸内T水平:实验组4周与对照组4周相比下降,但无统计学意义(P>0.05);实验组8周与对照组8周相比下降,具有统计学意义(P<0.01)。结论:在8周内实验性大鼠VC并没有引起血清T的降低,但可以导致双侧睾丸内T降低。     

胃癌螺旋CT与病理、nm23-H1蛋白表达的相关性研究

目的 探讨胃癌螺旋CT征象与手术病理及nm23-H1蛋白表达间的关系.资料与方法 对65例胃癌行低张力水充盈螺旋CT三期增强扫描,所有病例均行手术切除,术后标本采用免疫组织化学SP法检测肿瘤组织中nm23-H1蛋白表达.将螺旋CT诊断结果 与病理结果 、nm23-H1蛋白表达进行对照.结果 65例胃癌TNM分期CT的准确性为80.0%(52/65),nm23-H1蛋白阳性表达率为50.8%(33/65).CT像上的病灶大小、浆膜侵犯、淋巴结转移、TNM分期与病理结果 一致性良好,与nm23-H1蛋白阳性表达率均密切相关(P＜0.05).结论 螺旋CT可较准确地反映胃癌增殖、浸润转移的病理学及生物学特性.