340株铜绿假单胞菌感染及耐药性分析

目的 了解铜绿假单胞菌感染分布及其对不同类型抗菌药物的耐药情况,以指导临床合理用药. 方法 抽取2009年1～12月浙江省丽水市中心医院感染铜绿假单胞菌病例340例,分别对临床感染病原菌分布、细菌耐药性及临床用药情况等进行回顾性分析. 结果从痰标本中分离的铜绿假单胞菌菌株最多,阳性率为80.3%;对铜绿假单胞菌敏感性最高的是多粘菌素(97.1%),其次是阿米卡星(91.8%)、妥布霉素(83.2%);耐药性最高的是复方磺胺甲唑(97. 4%),其次是米诺环素(92.6%)、美洛西林(70.9%). 金属酶阳性菌株仅对多粘菌素B敏感,对其余抗菌药物表现高度耐药. 结论 医院感染铜绿假单胞菌的情况十分严峻,医生应合理选用抗菌药物,减少耐药菌株的产生.     

格列喹酮与格列齐特的临床疗效及不良反应比较

目的回顾性分析对比格列喹酮与格列齐特的临床疗效及不良反应。方法将丽水市中心医院内分泌科用过格列喹酮和格列齐特两种药物3个月以上的门诊和住院患者129例的病历资料及化验单进行统计分析,比较用药后空腹血糖、餐后2 h血糖及肝肾功能等指标。结果两组治疗3个月后空腹血糖均明显下降(P<0.05),格列齐特降空腹血糖效果优于格列喹酮,格列喹酮降餐后2 h血糖作用优于格列齐特;两组肝肾功能各项指标无大的波动,不良反应发生率无显著性差异。结论格列喹酮和格列齐特都有一定的降血糖疗效,格列喹酮不良反应较格列齐特少,且对肾功能具有一定的保护作用。     

MMP/TIMP表达失衡在肾细胞癌中的研究

目的应用组织芯片技术探讨基质金属蛋白酶-2（MMP-2）、基质金属蛋白酶-7（MMP-7）和金属蛋白酶抑制因子-2（TIMP-2）在肾细胞癌中的表达情况及相关性。方法采用免疫组化法（SP法）检测82例肾细胞癌和40例正常对照者中MMP-2、MMP-7和TIMP-2的表达情况。结果 MMP-2在肾细胞癌组中表达阳性率71.9%,高于对照组15.0%,MMP-7在肾细胞癌组中表达阳性率67.1%,高于对照组20.0%,并随组织学分级、淋巴结转移、临床分期增高表达增强（P〈0.05）;TIMP-2在肾细胞癌组中表达阳性率34.1%,略高于对照组30.0%,无统计学意义（P〉0.05）,并随组织学分级、淋巴结转移和临床分期增加表达降低（P〈0.05）;在肾细胞癌组中两两之间呈负相关。结论 MMP-2-7/TIMP-2失衡可能参与了肾细胞癌的发生发展过程,联合表达可用于判断肾细胞癌的恶性程度。     

Ⅱ型与Ⅴ型腓骨瓣修复跟骨缺失初期再造跟骨稳定性的比较

目的 比较Ⅱ型、Ⅴ型腓骨瓣重建跟骨缺损的临床效果.方法 建立Ⅴ型和Ⅱ型的简化模型,分别模拟采用Ⅱ型Ｖ型腓骨瓣重建跟骨缺损,以结构力学计算得到的位移作为评价足功能指标,进行理论分析进而比较两者的稳定性.结果 建立了Ⅱ型腓骨瓣与Ⅴ型腓骨瓣修复简化模型并得到分析后的理论数据.理论计算表明,Ⅱ型腓骨瓣的稳定性优于Ⅴ型.两种重建...     

Efficacy of prophylactic laser retinopexy in acute retinal necrosis: A systematic review and meta-analysis

International Ophthalmology - We performed a systematic review and meta-analysis to assess the role of prophylactic laser retinopexy in preventing rhegmatogenous retinal detachment (RRD) in acute...     

Relationship between pericardial fluid B-type natriuretic peptide and ventricular structure and function

BACKGROUND: We undertook this study to investigate the levels of pericardial B-type natriuretic peptide (BNP) and its relationship with ventricular structure and function. METHODS: Pericardial and plasma BNP concentrations were measured in 18 patients with congenital ventricular septal defect. RESULTS: The mean level of BNP in the pericardial fluid (324.8+/-137.3 pg/mL) was higher than the plasma (20.8+/-6.1 pg/mL) (p=0.03). Pericardial BNP was correlated with the plasma BNP (r=0.85, p<0.01). A good correlation was also found between the pericardial BNP and left atrial diameter, left ventricular end-diastolic and end-systolic diameter, left ventricular ejection fraction, right ventricular diameter and pulmonary artery systolic pressure (p<0.05). CONCLUSIONS: The levels of BNP in pericardial fluid were higher than in the plasma. Similar to plasma BNP, pericardial BNP is also related to the ventricular structure and function.     

海藻糖对凝血因子保护作用的研究

目的探讨在低温冻结贮存下,海藻糖对血浆凝血因子活性的保护作用。方法用新鲜混合血浆分装7小瓶,分别配制使海藻糖的终浓度为0,0.05,0.10,0.15,0.20,0.25,0.30 m ol/L,再分装在-20℃下保存30,60,120,240,360 d后,测定凝血因子Ⅷ、Ⅸ、Ⅺ的活性,并计算保护率。结果在-20℃保存360 d时,无海藻糖保护组凝血因子Ⅷ、Ⅸ、Ⅺ的活性分别为66.7%,80.8%与79.6%。当海藻糖浓度为0.15 m ol/L时,对凝血因子Ⅷ、Ⅸ、Ⅺ的活性保护作用最为理想,分别为80.1%,92.0%与96.4%。结论在低温冻结贮存下,海藻糖对血浆凝血因子活性有一定的保护作用。     

The effects of increasing occlusal vertical dimension on the deep masseter of rat at different ages

ObjectiveTo investigate the influence of increasing the occlusal vertical dimension (iOVD) on the fibre-type distribution and ultrastructure of deep masseter of rat at different ages.DesignA total of forty-eight male Wistar rats were divided into two groups according to age: ‘teenage’ group (n = 24, 1.5 months) and ‘young adult’ group (n = 24, 8 months). Both the teenage and the young adult rats were then randomly divided into the control group (n = 12) and the experimental group (n = 12). The occlusal vertical dimensions of the rats in the experimental groups were increased by placing composite resin on all maxillary molars. The fibre-type distribution and ultrastructure of the deep masseter were subsequently observed on day 7 and day 14 after iOVD.ResultsIn the teenage experimental group, the proportion of type IIa fibres increased, while the proportion of type IIb and type IIx fibres decreased by day 7 after iOVD (P < 0.05). However, no significant fibre phenotype transformation was observed in the young adult experimental group until day 14 after iOVD. In addition, the proportion of type IIa in the teenage experimental group was higher than that of the young adult experimental group on day 7 and 14 (P < 0.05). Under the transmission electron microscope, muscle fibre reconstruction and the compensatory increase in the number and volume of mitochondria appeared earlier in the teenage experimental group. The cellular traumatic reaction was less than that in the young adult experimental group.ConclusionThe teenage rat alters masseter muscle structure to a slower phenotype earlier and to a greater degree than that of the young adult rat when increasing the occlusal vertical dimension.