S1PR1 expression correlates with inflammatory responses to Newcastle disease virus infection

Newcastle disease virus (NDV) is the causative agent of Newcastle disease, which is characterized by inflammatory pathological changes in the organs of chickens. The inflammatory response to this disease has not been well characterized. Previous reports showed that the sphingosine-1-phosphate-1 receptor (S1PR1), a G protein-coupled receptor, is important to the activation of inflammatory responses. To understand better the viral pathogenesis and host inflammatory response, we analyzed S1PR1 expression during NDV infection. We observed a direct correlation between chicken embryo fibroblast (CEF) cellular inflammatory responses and S1PR1 expression. Virulent NDV-infected CEF cells also had elevated levels of pro-inflammatory cytokines (IL-1β, IL-6 and IL-18). When S1PR1 was inhibited by using the specific antagonist W146, pro-inflammatory cytokine production declined. Overexpression of S1PR1 resulted in increased virus-induced IL-1β production. S1PR1 expression levels did not impact significantly NDV replication. These findings highlight the important role of S1PR1 in inflammatory responses in NDV infection.     

乳腺结节状病变的MRI诊断

  目的  探讨乳腺良、恶性结节病变的MRI诊断价值。  方法  回顾性分析来芜湖市第一人民医院就诊的乳腺疾病患者71例，均经过病理证实结节状病灶，术前均进行MRI多参数成像，选取动态增强（DCE-MRI）和DWI数据进行分析，经处理得到:时间-信号曲线（TIC），达峰时间（Tpeak），峰值强化率（PER），峰值（SImax）及表观扩散系数（ADC），结合形态学特征:边缘毛糙、环形强化、边缘毛刺征及分叶征进行分析。  结果  恶性病灶43例，良性病灶 28例。TIC时间-信号曲线Ⅰ型22个，以良性为主17例，恶性5例；Ⅱ型35例，恶性为主24例，良性11例；Ⅲ型14个，均为恶性；良、恶性病灶TIC曲线类型有差异，差异有统计学意义（P < 0.05）；良、恶性病变的峰时（Tpeak），峰值强化率（PER），峰值（SImax）及ADC有显著差异，差异有统计学意义（P < 0.05）。边缘毛糙33例，均为恶性；环形强化24例，良性5例，恶性19例；毛刺征29例，均为恶性；有分叶征42例，良性7例，恶性35例。良恶性病灶形态学，差异有统计学意义（P < 0.05）。  结论  磁共振动态增强扫描血流动力学参数、ADC结合形态学特征对乳腺结节状良、恶性疾病的诊断具有重要价值。     

100例左右两侧基底神经节区出血致卒中后抑郁的对比研究

目的:观察左右两侧基底神经节区病变致脑卒中后抑郁发生情况的侧别间差异。方法:左侧或右侧基底神经节区出血的患者各50例,详细测查其情绪状况。结果:左侧50例合并卒中后抑郁39例,右侧50例合并卒中后抑郁14例,左侧高于右侧,差异有统计学意义。从汉密尔顿抑郁量表得分看,左侧基底神经节出血组的总分集中在26分左右,而右侧基底神经节出血组的总分集中在11分左右,左侧组得分明显高于右侧组。结论:左侧基底神经节损害合并卒中后抑郁发病率高于右侧,左侧基底神经节区出血致抑郁程度重于右侧。     

Baicalin from the extract of Scutellaria baicalensis affects the innate immunity and apoptosis in leukocytes of children with acute lymphocytic leukemia

Scutellariae Radix (root of Scutellaria baicalensis) has a long history of application in traditional and in modern herbal medications. The major components of Scutellariae Radix are baicalin, baicalein, wogonoside and wogonin. Accumulating evidence demonstrates that Scutellaria has immunomodulatory effects and possesses compelling anticancer potential.Treatment of peripheral blood leukocytes (PBLs) with Scutellaria extract (SBE) enriched in baicalin, reduced viability of PBLs obtained from patients with acute lymphoblastic leukemia (ALL). SBE had no impact on the survival of healthy, control leukocytes. The immune system modulation by SBE resulted in increased production of IFNγ in PBLs, and reduced TNFα and IL-10 production in bone marrow cells (BMC), in ALL patients. SBE stimulated the nonspecific antiviral immunity, assessed by resistance of PBLs and BMC to vesicular stomatitis virus (VSV) infection. SBE showed pro-apoptotic activity in NALM-6 cell line (B-type human leukemia). The number of cells expressing annexin V increased from 6% in control cultures to 29% and 52% after treatment with 100 μg/ml and 200 μg/ml respectively. Increased percentage of apoptotic cells was observed when cells were treated with corresponding concentration of baicalin. SBE enhanced apoptosis of PBLs in BMC of leukemic children. The percentage of PBLs that underwent apoptosis and mean annexin V expression increased from 11% in the control to 17% and 24% for the doses of 100 μg/ml and 200 μg/ml respectively. Importantly, SBE did not induce apoptosis of PBLs in the healthy, control group.     

药师处方权认知度和支持度的初步调查

目的：了解医护人员、药师和公众对药师"部分"处方权的认知度和支持度。方法：对医护人员、药师以及公众进行问卷调查。结果：医生、护士和公众对药师"部分"处方权非常清楚或比较清楚的分别占35.70%、36.24%、33.44%，药师占57.05%，医生、护士和公众对赋予药师"部分"处方权完全同意或同意的分别占82.01%、76.74%、83.12%，药师占94.82%，而且认知度与支持度在0.01水平上显著相关，不同年龄、学历、职业、职称、医院级别等的支持度有统计学意义。结论：医护人员、药师和公众对于药师"部分"处方权的概念、资格准入等内容认知不足，但认可赋予药师"部分"处方权的好处，并且对赋予药师"部分"处方权持积极态度，认知情况越好支持度越高；医护人员、药师和公众对于赋予药师"部分"处方权的支持度，存在"老年高于青年，高职称高于低职称，高级别医院高于低级别医院，学历差异较大"等现象。     

A 16-plex Y-SNP typing system based on allele-specific PCR for the genotyping of Chinese Y-chromosomal haplogroups

Y-chromosomal SNP (Y-SNP), with its stable inheritance and low mutation, can provide Supplementary information in forensic investigation. While commonly used Y-chromosomal STR haplotypes show their limitations, typing of Y-SNP would become a powerful complement. In this study, a 16-plex Y-SNP typing system based on allele-specific PCR (AS-PCR) was developed to discriminate four dominant Y-chromosomal haplogroups (C-M130, D-CTS3946, N-M231, and O-M175) and 12 predominant sub-haplogroups of O-M175 (O1a-M119, O1a1a1a-CTS3265, O1b-M268, O1b1a2-Page59, O2-M122, O2a1-L127.1, O2a1b-F240, O2a1b1a1-CTS5820, O2a2-P201, O2a2b1a1-M177, O2a2b1a1a1a-Y17728, O2a2b1a2-F114). A series of experimental validation studies including sensitivity, species specificity, male-female mixture and inhibition were performed. The discrimination of the typing system was preliminarily proved with a haplogroup diversity of 0.9239. Altogether, the Y-SNP typing system based on AS-PCR should be capable of distinguishing China’s dominant Y-chromosomal haplogroups in a rapid and reliable manner, thus can be employed as a useful complement in forensic casework.     

基于蒙医白脉理论探析帕金森病＊

帕金森病是以多巴胺能神经元减少导致的以静止性震颤、肌强直、运动迟缓和姿势步态障碍为主要临床症状的疾病。此病是蒙医白脉病的一种，又称彻彻热乎病。蒙医将人体的脉络分为白脉和黑脉。白脉病是白脉系统遭受致病因素的影响后所致的一系列疾病的总称，临床上很多疾病归属于“白脉病”。为更好的实现对蒙医优势单病种的规范化研究，本文基于白脉理论，探析蒙医对帕金森病的认识，以期为未来的研究提供理论支持。     

浅谈3D打印技术在复杂创伤骨科教学中的应用

目的对于复杂创伤骨科教学工作中应用3D打印技术的应用价值进行探讨。方法选取2018年1月-2019年1月本科学生60名作为此次研究对象,根据随机数字表法对所选60名学生加以分组,其中30名学生接受传统教学方案,将其作为对照组,剩余30名学生接受3D打印技术教学,将其作为研究组,对比两组学生接受不同教学方案之后的教学成绩。结果除专业兴趣程度外,两组学生接受不同教学方案之后的自我认知能力以及出科考试成绩对比差异有统计学意义(P<0.05)。结论针对复杂创伤骨科教学工作中应用3D打印技术效果理想,能够帮助教学工作顺利完成,显著提高学生动作操作能力,使学生良好的掌握复杂创伤骨科知识。     

Assessment of blood 2-aminothiazoline-4-carboxylic acid concentrations: Age and sex differences,and correlation with carboxyhemoglobin in fire victims

Recently, 2-aminothiazoline-4-carboxylic acid (ATCA), a cyanide (CN) metabolite, has been proposed as a stable diagnostic marker of CN poisoning. In this study, liquid chromatography coupled with electrospray ionization – tandem mass spectrometry was used to quantify ATCA concentrations in human postmortem blood samples, and differences in ATCA concentrations according to age and sex were determined. Both age and sex had significant effects on blood ATCA concentrations. Although ATCA concentrations exhibited an inverted U shape with increasing age in men, in women ATCA concentrations plateaued at around 40–59 years of age. There were significant differences between the sexes in ATCA concentrations for the 20–39 and 40–59 year age groups (P < 0.05 and P < 0.01, respectively). Correlations between ATCA concentrations and carboxyhemoglobin (CO-Hb) saturation were also examined in fire victims. ATCA concentrations increased significantly with increasing CO-Hb saturation (r = 0.382, P < 0.01). In addition, ATCA concentrations were also correlated to CN concentrations (r = 0.309, P < 0.05). The results of our study may provide novel information about the contribution of CN poisoning to the cause of death at fire scenes.