高强度聚焦超声联合人工液胸治疗不可切除小儿肝母细胞瘤短期疗效研究*

目的 探讨采用高强度聚焦超声(HIFU)联合人工液胸治疗不可切除的小儿肝母细胞瘤(HB)短期疗效。方法 2018年6月～2020年6月我院收治的不可切除的HB患儿48例,采用随机数字表法将患儿分为对照组24例和观察组24例,分别给予HIFU或HIFU联合人工液胸治疗,观察12 w。结果 在治疗12 w末,观察组有效率为87.5%,显著高于对照组的62.5%(P＜0.05);观察组血清ALT、AST和AFP水平分别为(72.6±26.1)U/L、(61.0±30.3)U/L和(43.1±5.3)μg/L,显著低于对照组【分别为(117.5±30.2)U/L、(116.6±37.8)U/L和(155.2±6.1)μg/L,P＜0.05】,而血红蛋白水平为(96.4±26.1)g/L,显著高于对照组【(90.7±23.6)g/L,P＜0.05】;两组血清IgM、IgG、IgA和补体C3、C4水平比较,差异无统计学意义(P＞0.05);在治疗期间,观察组发热、肋骨受损、皮肤烫伤、腹腔积液和创伤性湿肺等并发症发生率为4.2%,显著低于对照组的29.2%(P＜0.05)。结论 采用人工液胸保护下的HIFU治疗不可切除的HB患儿短期临床疗效确切,能有效降低并发症发生率,值得临床验证。     

太极拳对超重2型糖尿病患者治疗效果及体质指数的影响

目的探讨太极拳对超重2型糖尿病患者治疗效果及体质指数的影响。方法选取2018年6月—2019年1月该院内分泌科收治,且BMI指数≥24的2型糖尿病患者93例,随机分为试验组和对照组两组,试验组46例,对照组47例。对照组实施常规糖尿病健康指导,试验组则在此基础上要求患者进行为期8周的杨氏24式太极拳锻炼。观察并比较两组患者干预后的空腹血糖、餐后2 h血糖水平及糖化血红蛋白值;比较两组患者干预后的体质指数。结果经过为期8周的太极拳锻炼,试验组患者的空腹血糖、餐后2 h血糖水平及糖化血红蛋白值均显著低于对照组,差异有统计学意义(P<0.05);试验组患者的BMI指数显著低于对照组,差异有统计学意义(P<0.05)。结论太极拳锻炼可有效控制及改善超重2型糖尿病患者的代谢指标,降低患者的BMI指数,缓解其胰岛素抵抗。     

银杏叶提取物对右美托咪定麻醉小鼠催醒的作用

目的探究银杏叶提取物对右美托咪定麻醉小鼠催醒的作用。方法32只昆明种小鼠随机均分为对照组、阿替美唑组、低剂量组及高剂量组。各组小鼠腹腔注射1 mg/kg右美托咪定,90 min后分别注射2 mg/kg阿替美唑、50 mg/kg(低剂量)及100 mg/kg(高剂量)银杏叶提取物,评估小鼠镇静、镇痛水平,并记录苏醒时间。检测各组脑组织中Ca²⁺、Mg²⁺-ATP酶、一氧化氮(NO)、一氧化氮合酶(NOS)、鸟苷酸环化酶(cGMP)活性、Orexin A及Orexin B蛋白表达。结果麻醉给药95、120及180 min时,与对照组比较,阿替美唑组和高剂量组镇静、镇痛评分均降低(P＜0.05),且麻醉给药180 min时,阿替美唑组和低剂量组镇静、镇痛评分比较,差异有统计学意义(P＜0.05);与对照组比较,阿替美唑组和高剂量组苏醒时间缩短(P＜0.05),且阿替美唑组苏醒时间短于高剂量组(P＜0.05);与对照组比较,阿替美唑组和高剂量组Ca²⁺、Mg²⁺-ATP酶活性、NO、NOS、cGMP活性、OrexinA及OrexinB蛋白表达升高(P＜0.05)。结论银杏叶提取物对右美托咪定麻醉小鼠具有催醒作用,可能与提高Ca²⁺、Mg²⁺-ATP酶活性、促进NO/cGMP信号转导和Orexin系统有关。     

Berberine protects HK-2 cells from hypoxia/reoxygenation induced apoptosis via inhibiting SPHK1 expression

Renal ischemia reperfusion injury (RIRI) refers to the irreversible damage for renal function when blood perfusion is recovered after ischemia for an extended period, which is common in clinical surgeries and has been regarded as a major risk for acute renal failures (ARF) that is accompanied with unimaginably high morbidity and mortality. Hypoxia during ischemia followed by reoxygenation via reperfusion serves as a major event contributing to cell apoptosis, which has been widely accepted as the vital pathogenesis in RIRI. Preventing apoptosis in renal tubular epithelial cell has been considered as effective method for blocking RIRI. In this paper, we established a hypoxia/reoxygenation (H/R) injury model in human proximal tubular epithelial HK-2 cells. Here, we found increased SPHK1 levels in H/R injured HK-2 cells, which could be significantly down regulated after berberine treatment. Berberine has been reported to exert a protective effect on H/R-induced apoptosis of HK-2 cells. So, in our present study, we planned to investigate whether SPHK1 participated in the anti-apoptosis process of berberine in H/R injured HK-2 cells. Our study confirmed the protective effect of berberine against H/R-induced apoptosis in HK-2 cells through promoting cells viability, inhibiting cells apoptosis, and down-regulating p-P38, caspase-3, caspase-9 as well as SPHK1, while up regulating the ratio of Bcl-2/Bax. However, SPHK1 overexpression in HK-2 cells induced severe apoptosis, which can be significantly ameliorated with additional berberine treatment. We concluded that berberine could remarkably prevent H/R-induced apoptosis in HK-2 cells through down-regulating SPHK1 expression levels, and the mechanisms included the suppression of p38 MAPK activation and mitochondrial stress pathways.     

前韧带加强修复结合螺钉固定用于下胫腓不稳的生物力学特性分析

目的 建立踝关节骨折固定后遗留下胫腓前韧带损伤,用锚钉加强修复结合螺钉固定的有限元模型,比较其受力及变形情况.方法 选取1例正常男性青年志愿者踝关节CT图像,建立踝关节骨折固定后遗留下胫腓前联合损伤有限元模型,对损伤模型置入锚钉固定为实验组,使用螺钉固定为对照组.通过有限元分析计算,观察两组模型在各种载荷工况下的最大应...