基因乙肝疫苗与血源乙肝疫苗阻断HBV母婴传播长期效果的比较

本文对新生儿接种基因乙肝疫苗和血源乙肝疫苗10－12年后的血检结果进行分析,以评价基因乙肝疫苗和血源乙肝疫苗对HBsAg（＋）和HBeAg（＋）母亲之新生儿的长期保护作用的效果。结果显示：基因乙肝疫苗阻断HBV母婴传播具有良好的长期免疫记忆反应。     

新生儿接种乙型肝炎疫苗的远期效果观察

新生儿接种乙型肝炎疫苗的远期效果观察陆建华，朱源荣，倪正平，黄飞，陈建国一、疫苗接种：我们对１９８４年１２月至１９８５年１２月出生的婴儿，按０、１、６全程免疫程序接种ｍｅｒｃｋ乙型肝炎疫苗。３岁时再随机分成０、５、１０ｕｇ３个加强接种亚组。二、随访与...     

江苏启东有肝病史的肝癌高危人群14年前瞻调查的病因因素研究

应用单因素和多因素统计模型方法对江苏启东有肝病史的肝癌高危人群进行五次前瞻调查的病因学研究。筛选出的主要危险因素为性别、HBsAg、肝癌家族史和肝病类型。应用几种多因素回归模型对各主要危险因素的相对危险度及人群归因危险度和综合人群归因危险度作出估计;分析了这些因素对原发性肝癌发病作用的大小。提出了今后对该人群防治策略的依据。     

肿瘤高发现场工作评估问题探讨

阐述了肿瘤高发现场工作评估的重要性,探讨了评估的主要形式、评估内容、评估方法、评估原则等,对开展评估工作具有重要参考价值.     

HLA-A02基因型与中国高发区肝细胞癌的密切关联

目的：探讨ＨＬＡ－Ａ０２型及其亚型与启东肝细胞癌的相关性。方法：用ＰＣＲ－ＳＳＰ法分析启东肝细胞癌和正常人中ＨＬＡ－Ａ０２型及其亚型的分布。结果：１０３例经病理诊断的肝细胞癌中５７例为ＨＬＡ－Ａ０２型,其比例达到５５％,显著高于启东正常人群ＨＬＡ－Ａ０２型３２％（３３／１０２）的比例,Ｐ〈０．００１。同时揭示包括ＨＬＡ－Ａ０２型在内的ＨＬＡ－Ａ位点的等位基因多为杂合型。启东肝细胞癌ＨＬＡ－Ａ０２型     

乙肝疫苗母-婴阻断失败者病毒全基因变异分析

Objective To determine the factors responsible for failed postnatal immunoprophylaxis for hepatitis B virus(HBV) in Qidong, China. Methods Eleven children who developed into chronic HBV infection after receiving HBIG and HBV recombinant vaccines were recruited into the study. Eleven paired mothers with chronic hepatitis and other 6 mothers whose children successfully generated anti-HBs after im-munoprophylaxis were included as the control in the study. Full-length HBV DNA was amplified through ser-um sample by PCR method and underwent cloning and sequencing. HBV DNA level was quantified by real-time PCR. Results The mean levels of HBV DNA in mothers who had HBV DNA positive children and healthy children were ( 1.2 ×107± 3.1 × 106 ) copies/ml and ( 1.6× 107±8.8×106 ) copies/ml, respec-tively. There was no significant difference between the groups (P >0.05). Meanwhile, viral load in chil-dren was unrelated to that in their mothers (r2 =0.2429). In 11 HBV DNA positive children, 4(36.4% ) demonstrated amino acid substitutions in HBsAg "a" determinant region with 6 different types, I.e. T125A, I126T, Q129H, M133V, D144V and G145A. All of the mothers showed the wild-type sequence in "a" epitope, indicating surface escape mutants were not acquired from the initial infection, but developed under the immune pressure. The mutation rates after immunoprophylaxis for preS1, preS2, S, X, preC/C and P genes were 0.38%, 0. 22%, 0.27%, 0.17%, 0.11%, and 0.11%, respectively, nt2999-3157 in preS1, nt529-677 in S, nt1955-2016 in C, nt923-1001 and nt2489-2602 in P genes were among the hottest muta-tional spots throughout the HBV genome. Conclusion HBV mutation may occur in all the open readingframes after passive and active immunoprophylaxis. In addition to S gene, HBV preS and P genes could alsoassociate with the escape mutants.     

启东肝癌防治现场的建设和发展

启东肝癌防治现场经历40年的努力,通过坚持适合自身发展的举措,逐步打造成一个癌症防治研究的示范基地.继续巩固和发挥现场优势、利用和加强协作优势,是现场防治研究可持续发展的关键.     

乙型肝炎病毒感染与肝癌发生的31年随访研究

目的 研究乙型肝炎病毒(HBV)与肝癌发生的关系,评价HBsAg长期携带者发生肝癌的结局.方法 利用1976年建立的启东某社区15岁以上自然人群队列[血清筛检确定HBsAg、抗-HBs及丙氨酸转氨酶(ALT),并进行前瞻随访],该数据库与人群癌症登记资料、居民病伤死因资料链接核实,分析1977年1月至2007年12月12351名人群中肝癌等肿瘤的发生情况.结果 队列观察总人年数为355 305.0.HBsAg携带者中发生肝癌173例,发生率为361.55/10万;HBsAg非携带者发生肝癌95例,发生率为30.90/10万,两组比较RR=11.70(95%CI:9.06～15.19);其中男性和女性HBsAg携带者的RR值分别为12.30和10.46.两性各年龄组HBsAg携带者的肝癌发生率均高于非携带者.若以女性非携带者肝癌的发生率为1,则男性非携带者、女性携带者、男性携带者的RR值分别为3.07、10.46和37.76;该四组人群在31年中肝癌发生的累积率分别为0.86%、2.73%、10.22%和34.19%.非条件logistic回归模型分析显示性别(男性)、年龄、HBsAg、ALT为肝癌发生的显著影响因素,抗-HBs为保护因素.HBsAg携带与其他部位癌的发生未见有联系.结论 证实启东自然人群中HBsAg携带与肝癌发生的因果联系;针对HBV感染而采取干预措施是现场肝癌防治的重点.     

乙肝疫苗母-婴阻断失败者病毒全基因变异分析

Objective To determine the factors responsible for failed postnatal immunoprophylaxis for hepatitis B virus(HBV) in Qidong, China. Methods Eleven children who developed into chronic HBV infection after receiving HBIG and HBV recombinant vaccines were recruited into the study. Eleven paired mothers with chronic hepatitis and other 6 mothers whose children successfully generated anti-HBs after im-munoprophylaxis were included as the control in the study. Full-length HBV DNA was amplified through ser-um sample by PCR method and underwent cloning and sequencing. HBV DNA level was quantified by real-time PCR. Results The mean levels of HBV DNA in mothers who had HBV DNA positive children and healthy children were ( 1.2 ×107± 3.1 × 106 ) copies/ml and ( 1.6× 107±8.8×106 ) copies/ml, respec-tively. There was no significant difference between the groups (P >0.05). Meanwhile, viral load in chil-dren was unrelated to that in their mothers (r2 =0.2429). In 11 HBV DNA positive children, 4(36.4% ) demonstrated amino acid substitutions in HBsAg "a" determinant region with 6 different types, I.e. T125A, I126T, Q129H, M133V, D144V and G145A. All of the mothers showed the wild-type sequence in "a" epitope, indicating surface escape mutants were not acquired from the initial infection, but developed under the immune pressure. The mutation rates after immunoprophylaxis for preS1, preS2, S, X, preC/C and P genes were 0.38%, 0. 22%, 0.27%, 0.17%, 0.11%, and 0.11%, respectively, nt2999-3157 in preS1, nt529-677 in S, nt1955-2016 in C, nt923-1001 and nt2489-2602 in P genes were among the hottest muta-tional spots throughout the HBV genome. Conclusion HBV mutation may occur in all the open readingframes after passive and active immunoprophylaxis. In addition to S gene, HBV preS and P genes could alsoassociate with the escape mutants.     

以论文和成果评价肿瘤防治现场的作用

我国肿瘤防治现场始建于60年代,70年代发展和巩固了一批现场。高发肿瘤现场在国家及有关单位支持和协作下,做了大量的工作,基本上掌握了现场自然人群的死因和肿瘤动态。初步探索了高发肿瘤的流行病学和病因规律,进行了Ⅰ级、Ⅱ级预防研究,取得了一些成绩,成为我国肿瘤防治研究的特色之一。为了充分估计