脂多糖诱发小鼠急性肺损伤microRNA-199a表达及其对基因表达的调控

目的 观察脂多糖(lipopolysaccharide,LPS)诱发的小鼠急性肺损伤中,microRNA-199a(miR-199a)的表达变化及其对基因的表达调控.方法 通过小鼠气道向其肺内注入LPS,构建小鼠急性肺损伤模型,采用Northern blot 分析miR-199a的表达水平;构建miR-199a的过表达...     

Perioperative treatment for the central type carcinoma of lung by lung resection with bronchus-first cutting off through pericardium

Objective To investigate the perioperative treatment,the cause of death,and complications of the lung resection for therapy of central type carcinoma of lung.Methods we retrospectively analyzed the treatment and prognosis of 136 patients with central type carcinoma of lung using the bronchus-first lung resection through pericardium during May 1993 to December 2006.Results Total amount of complications in this group was 40 cases,among them,5 cases with pulmonary arterial bleeding,4 cases with respiratory dysfunction,3 cases with contralateral lung pneumonia,6 cases with heart dysfunction,15 cases with arrhythmia,2 cases with injury of recurrent laryngeal nerve,3 cases with chylopleura,and 2 cases with leaking of the bronchus nub.The total complication rate was 27.9%.3 cases died during perioperation,and the mortality was 2.2%.The older the patients were,the higher rate of complications occurred.Conclusion In this group of 136 cases of lung carcinoma,the frequent postoperative complications were the complications of respiratory system and cardiovascular system.The leading causes of death were respiratory dysfunction and heart dysfunction.According to our data,we concluded that the prevention and treatment should be performed during perioperation as follows:Airway should be intensively cared and prepared before operation.The ventilation should be kept unblocked after operation.Assistant ventilation should be performed if necessitated.For those with coronary heart disease or pulmonary heart disease active treatment should be given carefully.Efficient acesodyne and sedation and strictly monitoring of cardiograph should be given postoperatively,finding out the cause of arrhythmia for the correct therapy to protect the heart function.In addition,the indication of operation should be strictly predominated and the principle of operation should be kept on.     

中央型肺癌伴先天性心脏病外科治疗1例

病人男,49岁.刺激性干咳伴消瘦2月余.CT 示(图1)右肺上叶支气管口闭塞,管口周围软组织块影,远端肺不张.心脏超声示右房、室增大,房间隔缺损(35mm),肺动脉压54mmHg(7.20 kPa).纤维气管镜检见右上叶开口堵塞;活检病理找到癌细胞.     

克服异烟酸空冷器粘壁现象对策的分析与探讨

本文以异烟酸生产中粘壁现象为代表，对物质粘壁的工程问题提出了涂复全氟化合物的解决对策，从化学的基本原理出发进行了理论分析，并对对策的实施方法进行了探讨。     

伊潘立酮的合成研究

目的：优化抗精神病药伊潘立酮1的合成工艺以适用于工业化生产。方法以4-哌啶甲酸、乙酸、乙酸酐为起始原料,经酰胺化、酰氯化、傅－克酰基化反应、水解得到2,4-二氟苯基-4-哌啶基甲酮盐酸盐4,再经肟化、分子内环合、成盐得6-氟-3-（4-哌啶基）-1,2-苯并异唑盐酸盐2。2和4-（3-氯丙氧基）-3-甲氧基苯乙酮在碱催化下缩合得到伊潘立酮1。结果与结论对反应物配比、催化剂用量、反应温度及时间等因素进行了优化研究,使得该合成方法反应条件温和、易控制,副反应少,后处理简单,总收率可达34．9％（以4-哌啶甲酸计）,其结构经IR、1 H-NMR和MS确证。     

气管巨大癌肿斜行袖式切除"Z"字成形吻合术(附1例报告)

目的：探讨研究一种新的气管成形吻合术在气管肿瘤外科的应用价值。方法：对１例肿瘤的长度５．８ｃｍ的气管中段鳞癌的病例采用右胸后外切口，在切断右下肺韧带、环形切开右肺门整圈心包返折、充分游离隆突及左、右总支气管的基础上，设计在上下气管斜形断端的一侧行小范围间断缝合，使之气管长度适当加长、在此基础上再施行“Ｚ”字成形吻合术。结果：气管巨大癌肿切除后气管断端直接成形吻合获得一期愈合，随访一年，在此基础上再     

2,6——二氯苯酚的合成方法

按起始原料的不同,分类评述了2,6—二氯苯酚化学合成的一些方法。     

系统性红斑狼疮患者外周血T淋巴细胞激活状态及OX40、OX40L mRNA表达

目的 探讨系统性红斑狼疮（SLE）患者外周血T淋巴细胞激活状态及OX40、OX40L mRNA表达水平。方法 采用双色流式细胞仪测定SLE患者和健康人外周血CD38CD3分子表达,采用荧光定量逆转录聚合酶链反应（RT-PCR）测定外周血单一核细胞（PBMC）中OX40、OX40L mRNA表达水平。结果 SLE患者外周血CD38CD3分子表达水平显著高于健康人（t = 3.12,P < 0.05）。活动期SLE患者PBMC OX40、OX40L mRNA水平显著高于非活动期患者和健康人（F = 4.13,3.12,P值均 < 0.01）。OX40 mRNA水平与SLE疾病活动指数呈正相关（r = 0.35,P < 0.05）。结论 T淋巴细胞异常活化及OX40、OX40L mRNA表达水平上调在SLE的发生、发展中可能起一定作用。     

乳腺手术112例围术期抗菌药物使用情况调查分析

目的 调查乳腺手术围术期抗生素应用现状,探讨提高抗菌药物合理应用水平.方法 对112例乳腺手术围术期抗生素使用情况进行回顾分析.结果 112 例乳腺手术患者围术期抗生素使用率为100.0%,其中预防性用药83例占74.1%,治疗性用药29例占25.9%.初次给药时机选择不合理38例术中无1例追加抗生素,手术后当天100.0%使用抗生素.大部分病例选择第1代头孢类抗生素.结论 乳腺手术围术期抗生素使用仍存在术前用药时机选择不当、预防用药时间过长等问题,应继续加强对围术期抗生素使用的管理,规范其应用.