cDNA微阵列技术检测胃肠安复方对人胃癌细胞基因表达谱的影响

目的:观察健脾类中药胃肠安复方对人胃癌细胞裸小鼠移植瘤的抑瘤作用,探讨基因表达谱芯片在中药复方对胃癌细胞多基因表达影响研究中的应用.方法:采用人胃癌细胞SGC-7901裸小鼠皮下移植瘤模型.造模1周后选瘤块直径1.5 mm以上者共16只,随机分为胃肠安复方组及0.85%氯化钠溶液对照组,每组8只.造模后41 d处死动物.通过RT-PCR反转录、同位素标记、全基因表达谱芯片方法研究胃肠安复方组与对照组人胃癌移植瘤细胞mRNA的表达差异.结果:胃肠安复方组瘤重低于对照组[(0.99±0.49)g:(1.93±0.52) g,P<0.05],胃肠安复方组抑瘤率为48.64%.全基因表达谱芯片检测比较分析示,胃肠安复方组与对照组样品间共有45个显著性差异表达基因,其中上调表达24个,下调表达21个,35个已克隆基因,10个表达序列标签.结论:健脾中药胃肠安复方抑制人胃癌细胞裸小鼠移植瘤生长,并可能对肿瘤细胞多基因表达有影响.     

晚期恶性肿瘤的异病同治——健脾法在消化道恶性肿瘤中的应用

应用中医中药治疗晚期恶性肿瘤,特别是消化道恶性肿瘤时,尽管用药不同,但有一种方法是常被采用的,这就是健脾法。在胰腺癌、贲门癌、肝癌等患者身上应用同一法则而取得不同程度的疗效,这不能不承认中医异病同治的理论确是令人信服的。     

健脾理气、清热解毒、软坚化痰中药对二乙基亚硝胺致大鼠肝癌的作用

在二乙基亚硝胺诱发大鼠肝癌的过程中测定肝内酶 ACT、γ-GT、OCT、CPS_1以及 AKP,并作病理检查,观察灌喂健脾理气、清热解毒、软坚化痰方剂的中药组、西药组(Vit.C Vit.E Vit.A)和对照组的酶以及病理变化。结果:对照组在致癌过程中 AKP、ACT、γ-GT 增高,而 CPS_1、OCT 下降,中药能改变这种情况。西药组与中药组γ-GT、ACT、CPS_1存在显著差异(P<0.05)。病理证实:肝组织脂变、纤维间变、假小叶形成等变化以对照组最严重、西药组次之,中药组最轻。     

SRRS方剂抑制消化道恶性肿瘤P53基因突变的实验研究

 目的：从分子水平探讨SRRS方剂治疗消化道癌的可能机制。方法：选用Lovo结肠腺癌细胞裸小鼠移植瘤模型,观察中药SRRS方剂抑制移植瘤生长的作用,并用PCR-SSCP银染色法研究SRRS方剂对Lovo移植瘤生长过程中P⁵³基因突变的影响情况。结果：对照组有50%(5/10)发生P⁵³基因第七外显子突变,SRRS方剂治疗组无(0/10)此现象(P<0.05)。结论：提示SSRS方剂能抑制lovo移植瘤生长过程中P⁵³基因的突变,即SRRS方剂可对肿瘤生长过程中肿瘤细胞的基因水平有影响作用。     

中医中药治疗恶性肿瘤服药方法探讨

中医中药治疗恶性肿瘤疗效肯定,但目前服汤药大多按一日2次服用,其间隔时间一般为5～6小时。在临床实践中,我们发现汤药服法不同,常影响疗效,因此开展了这方面的实验研究工作。一、研究方法及结果研究方法采用集落残存法,采用人体胃癌细胞株,选用党参、白术、茯苓、丹参、菝葜、红藤、生牡蛎、     

INFLUENCE OF MEDICINAL HERBS DECOCTED WITH DIFFERENT UTENSILS ON COLONY FORMATION OF GASTRIC CARCINOMA CELLS

in order to elucidate the different resultsobtained in cancer patients with similar condition andsymptoms treated by the same medicinal herbs,an in-vestigation of the utensils used for making decoctionswas carried out.it was found that the decoction madeby mcans of glassware,enamel and earthenware potshad the best effect of Inhibiting the colony formationof human gastric carcinoma cells,the next were thedecoctions made by means of unrefined iron pots,stainless steel pots and copper pots,and the worstwas that made with aluminium pots.It was alsofound that there was no difference between the watercontained in those utensils and normal saline in theinfluence on the colony formation of human gastriccarcinoma cells.Therefore,it is believed that the dif-ference in effect of the decoctions made by means ofdifferent kinds of utensils is not due to the tracedissolution of the utensil materials,but is most likelydue to the occurence of some chemical reactions whilemaking the decoction.That the decoctions made bymeans of different utensils had different peak valuesin the absorption spectrum also supports this pro-position.