凝血块洗下红细胞对血型鉴定的影响

目的分析不抗凝标本凝血块洗下红细胞、抗凝标本血浆对ABO血型鉴定的影响。方法采集723例受血者不抗凝与抗凝标本各1管,用盐水试管法进行ABO正、反血型鉴定。结果 723例不抗凝标本凝血块洗下红细胞做正定型,镜下有6例出现凝集,占0.83%(6∕723);抗凝标本血浆做反定型,有432例出现缗钱状凝集,占59.75%(432∕723)。结论不抗凝标本凝血块洗下红细胞对ABO正定型有影响;抗凝标本血浆对ABO反定型的影响显著。因此,选择用抗凝标本的红细胞做正定型,用不抗凝标本的血清做反定型,实验结果准确度更高。     

两种膨润土细胞毒性的体外试验研究

Objective To study comparatively the cytotoxicity induced by acid bentonite and organic bentonite.Methods The cytotoxicity of two kinds of bentonite was detected using CCK8 assay,neutral red uptake(NRU) assay,lactate dehydrogenase(LDH) leakage assay,apoptosis assay and hemolysis assay.In hemolysis assay human erythrocytes served as target cells and were exposed to the two kinds of bentonite at the doses of 0,0.3125,0.6250,1.2500 and 2.5000 mg/ml for ten min.In other four assays,human B lymphoblast cells (HMy2.CIR) served as target cells and were exposed to the two kinds of bentonite at the doses of 0,10,20,30,60,120 and 180 μg/ml for four h.Results In hemolysis assay,the hemolysis rates induced by two kinds of bentonite at all doses were significantly higher than that of control (P＜0.05);in CCK-8 assay,the cellular activities in acid bentonite group at the doses ≥30 μg/ml and in organic bentonite group at the doses ≥20μg/ml were significantly lower than that of control (P＜0.01);the similar results appeared in NRU assay and LDH assay,and the dose-effect relationship was observed in above 4 assays.In apeptosis assay,the early apoptosis cell rates in acid bentonite group at the dose of 180 μg/ml and in organic bentonite group at the doses of 120,180 μg/ml were significantly higher than that of control (P＜0.05).Moreover,the results of five in vitro assays indicated the eytotoxicity induced by organic bentonite was higher than that induced by acid bentonite.Conclusion Two kinds of bentonite could induce cytotoxicity,such as apoptosis and damage of cell membrane.The cytotoxicity of organic bentonite is higher than that of acid bentonite due to the different industrial treatment and characteristics of two kinds of bentonite particles.     

皮肤疝2例

<正>1病例资料例1.患者男，55岁。因腹部皮下包块10年就诊。患者10年前无明显诱因腹部出现一皮下包块，无疼痛及瘙痒等不适。2个月前皮下包块出现隐痛，遂于2020年7月27日来我院就诊。既往史无特殊，家族中无类似疾病患者。体格检查：一般情况良好，心脏及肺部均正常，腹肌稍紧张，全腹无明显压痛、反跳痛。皮肤科检查：肚脐上方约5 cm处触及一4 cm×3 cm包块（图1A），质中，     

3D打印生物陶瓷材料在口腔修复领域的研究进展

生物陶瓷材料的传统制造工艺主要有以数控切削成型为代表的减材制造法和以失蜡铸造为代表的等材制造法。近年来，随着3D打印技术的快速发展，增材制造技术被用于加工生物陶瓷材料，逐渐成为口腔修复领域的研究热点。该文从3D打印生物陶瓷的研究进展及其在口腔修复中的应用现状进行综述，并对未来发展进行展望。     

沧州市区2010—2019年甲状腺癌发病时间趋势和生存分析

目的 对沧州市区2010—2019年居民甲状腺癌发病时间趋势和生存分析进行研究，为沧州市区甲状腺癌的防治工作提供参考。方法 收集2010—2019年沧州市区甲状腺癌发病的监测数据，统计甲状腺癌发病率、标化率、发病率的年度变化百分比(APC)、平均生存时间、累积生存率等。结果 沧州市区2010—2019年甲状腺癌平均年发病率为18.64/10万，中标率为17.60/10万。女性发病率高于男性(P<0.05)。20～岁以上组发病率快速升高，在50～岁组达到峰值，后随年龄增加发病率逐渐下降。2010—2014年，甲状腺癌发病率呈上升趋势(APC=41.95%,95%CI:3.68%～94.35%,P=0.035)。甲状腺癌患者平均生存时间为124.29个月，5年、10年累积生存率分别为93.2%、90.4%。不同性别间生存曲线无统计学差异(P>0.05),65岁以下患者组甲状腺癌生存状况较65岁及以上患者组更好(P<0.05)。结论 甲状腺癌对沧州市区居民的健康威胁日益加重，应通过综合干预手段减轻因甲状腺癌导致的居民疾病和经济负担。