猫视皮层ERK mRNA表达的研究

丝裂原激活的蛋白激酶又称细胞外信号调节激酶,具有神经元信号传导功能,参与多种发育过程。它由细胞外特殊信号激活,将酪氨酸磷酸化过程转化为丝氨酸／苏氨酸磷酸化过程,后者进一步调节下游的蛋白激酶。因此,细胞外信号调节激酶在磷酸化的连锁反应过程中起着枢纽作用。本文应用地高辛标记寡核苷酸探针的原位杂交技术,研究了细胞外信号调节激酶的２ 种亚型 ＥＲＫ１ 和ＥＲＫ２ ｍ ＲＮＡ 在生后４ 周龄的幼猫视皮层各层的分布。结果表明,ＥＲＫ１ ｍ ＲＮＡ 在视皮层的表达层次明确,以第Ⅴ层和第Ⅲ层的表达为最强,Ⅱ层呈中等表达,Ⅳ层较弱,Ⅵ层最弱,即在视皮层的表达强度为Ⅴ、Ⅲ＞ Ⅱ＞ Ⅳ＞ Ⅵ。与ＥＲＫ１相比,ＥＲＫ２ 的表达普遍较强,但层次欠清晰,其表达强度为Ⅴ、Ⅱ＞ Ⅲ、Ⅳ＞ Ⅵ。ＥＲＫ 的２ 种亚型在视皮层的Ⅰ层即分子层均不表达。ＥＲＫ１ 和ＥＲＫ２ ｍ ＲＮＡ 在视皮层的这种特征性分布提示,在幼猫的视皮层发育过程中,ＥＲＫ 可能具有独特的信号传递作用。     

角膜和全眼高阶波前像差的特点及年龄相关性变化的研究

Objective To investigate age-related changes in corneal and ocular monochromatic high-er-order aberrations and elucidate the distribution of corneal primary spherical in Chinese. Methods Using Traeey-iTrace visual function analyzer (EyeSys corneal topography combined with a ray-tracing aberrometer),113 normal eyes of 59 Chinese,mean age of 33.81+20.50 years (range,5-73 years),were evaluated for corneal and ocular monochromatic total higher-order aberrations (HOAs), primary spherical aberration (Z40),total spherical (TS),total coma (TC),and total trefoil aberration (TT) in the central 6mm diameter. Correla-tion analysis was also performed to assess the association between age and both corneal and oeular higher-order aberrations. Results The mean Zemike coefficient of Z40 was (0.27±0.07) ?m (range,0.130～0.451). Al-though corneal Z40 increased slightly with age,the correlation was small and insignificant (r =0.172,p =0.069 ). However, ocular Z40 had a positive correlation with age (r =0.545,p =0.000 ). Analyzer showed positive correlations with age for both corneal (r=0.499,p =0.000) and ocular (r=0.427,p =0.000) HOAs. Positive correlations were also observed between age and both corneal (r=0.149,p =0.021 ) and ocular (r=0.253,p =0.007) TC. Corneal and ocular TT (r =0.344,p =0.000; r=0.185,p =0.050,respectively) were increased with age. No significant correlation was found between corneal TS and age (r =0.180,p =0.057 ), but TS of the whole eye in-creased with age (r =0.560,p =0.000). There was a positive correlation between corneal and ocular HOAs (r =0.327,p=0.000),TC (r=0.233 ,p=0.013),and TT (r=0.481 ,p=0.000) aberration. Conclusions These re-suits also suggest that the ocular HOAs,TC,and TT increase with age,mainly because of the increase in corneal HOAs,TC,and TT; and the ZAO aberration increases with age,mainly because of the increase in Z40 in the internal optics. Individual variability in corneal primary spherical aberration was observed in Chinese.Therefore, preoperative measurement of corneal Z40 is crucial to the selection of aspheric IOL.     

角膜炎、鱼鳞病、耳聋综合征的眼部表现

目的 观察角膜炎、鱼鳞病、耳聋综合征的临床表现。方法 报告1例角膜炎、鱼鳞病、耳聋综合征患者的眼部表现。结果 患者的眼部表现为角膜混浊、角膜血管翳。结论 系统应用异维A酸、局部应用泰利必妥和2％环孢霉素滴眼剂后眼部改善症状。     

颈部多普勒超声联合经颅多普勒超声在老年急性脑梗死患者颈动脉狭窄评估中的价值

目的 探讨颈部多普勒超声（CDU）联合经颅多普勒超声（TCD）在老年急性脑梗死患者颈动脉狭窄评估中的价值。方法 选取170例老年急性脑梗死患者，均进行CDU、TCD检查，并以数字减影血管造影（DSA）为金标准，分析CDU联合TCD对颈动脉狭窄的评估价值。结果 TCD检出颅内血管狭窄48例（28.24%），颈内动脉颅外段狭窄78例（45.88%），脑动脉硬化44例（25.88%）。CDU对颈动脉狭窄的检出符合率93.53%(159/170)。随着颈动脉狭窄程度越明显，颈动脉收缩期峰值、舒张期末流速（PSV、EDV）值越高，大脑动脉阻力指数（RI）值越高、平均血流速度（Vm）越低（P均<0.05）。CDU、TCD联合指标对颈动脉重度狭窄诊断灵敏度为92.20%、特异度为95.50%，均高于CDU、TCD单一检测（P均<0.05）。结论 CDU联合TCD对老年急性脑梗死患者颈动脉狭窄有较高的评估价值。     

预警评分对新型冠状病毒肺炎住院患者转入ICU的预测价值

评估预警评分对新型冠状病毒肺炎(COVID-19)住院患者转入ICU的预测价值。选2021年1-2月在石家庄市人民医院住院的368例COVID-19患者, 记录患者入院当日的英国国家早期预警评分(NEWS)、英国国家早期预警评分2(NEWS2)、快速急诊内科评分(REMS)、快速序贯器官功能衰竭评分(qSOFA)、改良肺部炎症评分(CURB-65), 采用受试者操作特征曲线(ROC)分析上述评分对住院COVID-19患者是否转入ICU的预测价值。结果显示, 368例患者中32例(8.7%)转入ICU, 年龄49.0(34.0, 61.0)岁。入院当日的NEWS为1(0, 2)分, NEWS2为1(0, 2)分, REMS为4(2, 6)分, CURB-65为0(0, 1)分。预测患者转入ICU NEWS的ROC曲线下面积(AUC)为0.756, 敏感度为65.6%, 特异度为71.3%;NEWS2的ROC AUC为0.732, 敏感度为62.5%, 特异度为61.3%;REMS的ROC AUC为0.787, 敏感度为84.4%, 特异度为64.6%;CURB-65的ROC AUC为0....