EPO预处理对培养心肌细胞缺氧/复氧损伤TNF-α表达的影响及机制

目的 观察促红细胞生成素（erythropoietin，EPO）预处理对培养心肌细胞缺氧复氧前后TNF-α表达的影响，并进一步研究其可能的NF-kB信号机制。方法采用培养乳鼠心肌细胞建立缺氧复氧损伤模型，分为对照组、EPO组[缺氧复氧前24h，培养液中加入终浓度10U/ml的重组人促红素（recombinant human erythropoietin，RHuEPO）]、EPO＋吡咯烷二硫氨基甲酸盐（PDTC）组（缺氧复氧前24h，加入终浓度10U／ml的RHuEPO和5μg/ml的PDTC）和PDTC组（缺氧复氧前24h，加入终浓度5μg/ml的PDTC）。分别于缺氧复氧损伤前后，以RT-PCR和western blot检测各组心肌细胞TNF-α基因表达变化，同时以EMSA检测各组心肌细胞NF-kB活性变化。结果缺氧复氧损伤前，各组心肌细胞TNF-α基因表达水平差异无统计学意义，均较弱。损伤后各组心肌细胞TNF-α基因表达水平较损伤前对照组显著升高（P〈0．01），而EPO组TNF-α基因表达水平低于其他各组（P〈0．01）。缺氧复氧损伤前EPO组NF-kB活性高于其他各组（P〈0．01），损伤后各组NF-kB活性显著高于损伤前对照组（P〈0．01），EPO组NF-kB活性低于其他各组（P〈0．01）。结论EPO预处理抑制缺氧复氧后心肌细胞TNF-α基因表达升高，可能与缺氧复氧后NF-kB活性升高被抑制有关。EPO预处理可能通过NF-kB活化的负反馈机制抑制缺氧复氧后心肌细胞NF-kB活性的升高。     

Goldenhar 综合征家系的临床表型和遗传学分析

目的分析1组Goldenhar综合征家系的临床表现及遗传学特征。方法我们随访到1组4代33人的Goldenhar综合征家系，对目前存活的29人进行了临床表型和遗传学的初步分析。结果家系内有Goldenhaar综合征患者5人．临床表现具高度多样性，累及眼、耳、脊柱、颜面、口腔等多个器官和系统的发育不良，在遗传方式上属于常染色体显性遗传。从细胞遗传学水平对家系中成员进行染色体检查，未发现核型异常。结论该Goldenhar综合征家系属常染色体显性遗传，染色体检查未发现核型异常。     

Immunoreactivity of sinusoids in hepatoblastoma: an immunohistochemical study using lectin UEA-1 and antibodies against endothelium-associated antigens, including CD34

Sinusoids are found not only in the normal liver but also in certain liver tumours, including hepatoblastoma, the most common malignant liver tumour in childhood. In this study, sinusoids in 12 hepatoblastomas, of various subtypes, and in normal liver were investigated with UEA-1 and antibodies against von Willebrand's factor, CD31 and CD34 to detect differences of possible diagnostic significance. In the normal liver, staining of sinusoids was seen with all these markers, but it was focal and confined to a few sinusoids near the portal tracts. In hepatoblastoma, the endothelial markers reacted with the sinusoids to varying extents. UEA-1 and anti-CD34 usually stained the majority of these vessels, anti-CD34 staining greater numbers of sinusoids and with greater intensity. Immunostaining revealed that both number and spatial organization of sinusoids in hepatoblastoma are dependent on the subtype. In addition to staining of endothelium, one of the two small cell hepatoblastomas exhibited strong immunoreactivity of the tumour cells for CD34. These findings show that the marked difference in sinusoidal immunoreactivity for CD34 between normal liver and hepatoblastoma could be useful for discriminating between non-neoplastic liver tissue and highly differentiated fetal hepatoblastoma. Our findings also show that small cell hepatoblastoma, in addition to acute leukaemia, should be considered when immunoreactivity for CD34 is found in small round and blue cell tumours in childhood.     

组织扩张术皮肤胶原的代谢改变

目的：研究常规扩张（ＩＴＥ）和持续快速扩张（ＣＴＥ）对皮肤胶原代谢的影响。方法用白色小家猪制作组织扩张术动物模型，用Ｇｏｒｄｅｌａｄｚｅ法测定血清和扩张组织的羟脯氨酸（ＨＰ）含量，藻酸盐印模材膜片法测量标记区面积；光镜测量真皮厚度。结果：ＩＴＥ组血清ＨＰ含量升高，０．８倍，ＣＴＥ组升高１．２倍，ＩＴＥ和ＣＴＥ组皮肤含量与正常皮肤相同，组织中ＨＰ总量均明显升高，持续扩张皮瓣组（ＣＴＥＦ）术后４ｗｋ皮     

长期居住海平面后藏族经受急性减压低氧时的体力活动能力

本研究从急性低氧对移居海平面后藏族的体力活动能力的影响来探索藏族的高原低氧适应机制。结果揭示藏族的最大体力负荷强度没有显著下降，最大氧耗和氧脉搏也没有明显变化，动脉血氧饱和度明显高于汉族，这更显示藏族对高原低氧适应机制有独特之处。推测可能是藏族在氧的摄取、传递和释放过程比汉族更有利于对高原低氧环境的适应。     

低能量氦—氖激光局部加穴位照射治疗肺结核空洞

目的探讨低能量氦－氖激先局部加穴位照射对肺结核空洞的治疗作用机理。方法９１例初治Ⅲ型肺结核（肺部有空洞）随机分为实验组４６例，对照组４５例，均用６个月短程化疗方案．实验组加氦－氖激光局部、双肺俞穴位照射治疗３个疗程，观察空洞闭合及痰菌阴转情况，以Ｘ２检验分析组间治疗结果的差异．结果实验组治疗２、３个月空洞闭合分别为５８．３％、７０．８％；对照组为３５４％、４７９％，二者均有显著性差异（Ｐ＜０．０５），实验组痰菌阴转、肺部病灶吸收、临床症状改善均高于对照组。结论低能量氦－氖激光局部加双肺俞穴位照射，可加速肺结核空洞的闭合及临床症状的改善     

肝VX-2 瘤模型MR扩散成像的实验研究

目的探讨肝VX-2瘤模型MR扩散成像特征.方法新西兰大白兔35只,采用块种植的方法,一期预实验皮下种植14只,肝内种植6只;二期肝内种植12只,另3只做正常对照.对包括二期肝内种植在内的15个肿瘤于种植前后行定期扩散加权成像(DWI) 与MR检查.以表观扩散系数(ADC)值等为指标进行统计分析.结果 (1)一期预实验,皮下种植成功率29%(4/14),肝内种植成功率33%(2/6);二期肝内种植成功率83%(10/12).(2)VX-2瘤在DWI上呈高信号,边缘清楚.正常组与VX-2瘤实验组b值为100和300 s/mm2时ADC值分别为(2.57±0.26)mm2/s、(1.73±0.31)mm2/s、(1.87±0.25)mm2/s与(1.57±0.23)mm2/s(F=43.26,P<0.001).随b值增大,病灶信号降低;ADC图上病灶呈低信号;不同b值之间病灶ADC值差异有统计学意义(P<0.05);b=100 s/mm2病灶与正常肝脏之间ADC值差异有统计学意义(P<0.01).(3)VX-2瘤发展迅速,易发生肺、肝、纵隔等处转移.结论 DWI在反映肝VX-2瘤内部水分子运动、发现与追踪病灶进展等方面有重要价值.     

重组人红细胞生成素治疗肾性贫血的临床分析

目的　观察重组人红细胞生成素 (RhEPO)对肾性贫血的治疗作用。方法　根据使用EPO剂量的不同将 39例慢性肾衰竭并血液透析病人分成 4组 ,观察治疗后 2、4、12个月时与治疗前 (0月 )相比红细胞数 (RBC)、红细胞比积 (Hct)及血红蛋白含量 (Hb)的变化。结果　 2 4例使用EPO10 0 - 15 0IU/ (kg·w) (6 0 0 0IU/w - 90 0 0IU/w) ,治疗后RBC、Hct、Hb较治疗前有显著升高 (P≤ 0 .0 0 1) ;5例使用EPO5 0IU/ (kg·w) (30 0 0IU/w) +间断输血患者 ,其RBC、Hct、Hb升高不显著 ;10例不用EPO而单纯输血患者 ,其RBC、Hct、Hb无明显变化 (P >0 .0 5 )。结论　EPO能较好地纠正肾性贫血 ;单纯输血不能治疗肾性贫血     

XML技术在武汉市公共卫生信息网络系统中的应用

在研发武汉市公共卫生信息网络系统中,为解决不同疾病报表的填报问题,需要考虑不同报表的不同需求,统一网络系统报表管理体系,为适应这种特别要求,传统的关系数据库的方法,需要对多个报表单独设计,这样后台运数量会非常大,对硬件要求非常高,为解决这一突出问题,我们选择XML技术应用到系统研发,探索XML技术在公共卫生信息网系统中应用。1方法1.1依托ORACLE数据库和XML文件集构成统一的数据库平台。1.2采用XML统一表示来自不同数据源的数据,实现系统间的数据交换,实现信息共享。1.3总体研究方案见图1。图1总体方案2结果2.1通过采用虚…     

匹罗卡品致(癎)大鼠海马中表达生长抑素的中间神经元数目变化及其轴突出芽

Objective To investigate the roles of somatostatin(SS)positive intemeurons in the development and compensation of temporal lobe epilepsy.Methods Piloearpine-induced epilepsy rat model was established.Immunohistochemistry method was used to detect number changes and axonal sprouting of SS positive intemeurons in different domains of the hippocampus at difierent time points.Degeneration of SS positive interneurons and their neurophils were detected by the double immunofluorescence staining with SS and Fluoro-Jade B(FJB)at 7 and 60 days after status epilepticus (SE).Results In the exoerimental rat group,the number of SS positive neurons decreased in each hippocampal domain,and it reached the lowest at 7 days post-SE(There were 11.1±3.3 in hilus,2.8±0.9 in CA1region and 1.8±0.7 in CA1region,t=13.519,9.644 and 8.808,all P<0.01).In chronic phase,the number of SS neurons gradually recovered,and exceeded the control group in CA1 area at 60 days post-SE(12.8±1.5 vs 8.8±1.3,t=-4.506,P<0.01),however,the number of SS neurons in the hilus(25.5±4.6)and CA1 area(4.8±0.8)remained significantly less than normal levels(t value were 4.691 and 3.953.both P<0.01).Increased SS positive fibers were found in the lacunosum-molecular (1m)layer and outer molecular layer of dentate gyrus after 30 days post-SE,and numerous SS positive fibers were seen threnghout the layers of area CA1 at 60 days post-SE.Double immunofluuorescence revealed that a few SS positive interneurons and fibers were also labeled by FJB in area CA1 at 7 days post-SE and in CA domain/hilus at 60 days post-SE.Conclusions SS intemeurons loss plays an important role in the development of temporal lobe epilepsy.The loss is partially caIlsed by the degeneration and death of neurons;SS positive neurophils increase within area CA1 in chronic phase may play a significant role in the generation and compensation of temporal lobe epilepsy.