颈脊髓压迫模型的建立及意义

目的 :旨在探索、建立颈脊髓压迫损伤的动物模型 ,并观察、研究其病理学改变过程 ,为临床治疗颈脊髓损伤提供理论依据。　方法 :通过在犬C4 、C5椎间孔置入不锈钢螺钉压迫脊髓 ,成功建立起一种新型不完全性颈脊髓压迫损伤的动物模型 ,并通过光镜和电镜研究创伤后不同时间脊髓的病理改变。　结果 :损伤后动物表现为不完全性瘫痪 ,随时间的推移运动功能逐渐恢复。病理改变结果可分三期 :早期脊髓内出血、水肿及神经元和神经纤维变性 ;中期神经元和神经纤维变性达到高峰 ,胶质细胞增生 ;晚期出现机化和修复 ,部分神经元和神经纤维恢复正常形态。　结论 :颈脊髓不完全性压迫损伤后脊髓功能有恢复的可能 ,临床应进行早期治疗。     

Effect of Prevention and Treatment of Murine Acute Viral Myocarditisand Protection of Lymphoid Organ Atrophy withXinkang Oral Liquid (心康口服液)

Ｉｔｈａｓｂｅｅｎｅｓｔａｂｌｉｓｈｅｄｔｈａｔｃｏｘｓａｃｋｉｅｖｉｒｕｓｉｓｔｈｅｐｒｅｄｏｍｉｎａｎｔｃａｕｓｅｏｆｖｉｒａｌｍｙｏｃａｒｄｉｔｉｓｉｎｈｕｍａｎ(1) ,ｗｈｉｃｈｉｓａｃｏｍｍｏｎｄｉｓｅａｓｅｓｅｒｉｏｕｓｌｙｅｎｄａｎｇｅｒｈｅａ     

SOD-like Activity of Copper Salicylate Complex in Tween Micel-lar Systems

合成了水杨酸铜络合物并分析了其组成，提纯了Ｔｗｅｅｎ－２０，－４０，－６０，－８０，并测定了它们在ｐＨ７．４的磷酸缓冲液中的ＣＭＣ。用Ｃｙｔ．ｃ还原法分别测定了水杨酸铜络合物在不同介质中的ＳＯＤ样活性。详细研究了表面活性剂与水杨酸铜络合物协同清除Ｏ－２的作用。观察到在胶束体系中水杨酸铜络合物的ＳＯＤ样活性明显高于缓冲液体系无表面活性剂时的ＳＯＤ样活性，表面活性剂本身也具有清除Ｏ－２的功能。抗红细胞膜脂质过氧化实验也得到类似结果。这可用胶束催化解释。水杨酸铜－Ｔｗｅｅｎ体系可能是一种潜在的抗炎药物。     

8kg以下婴儿室间隔缺损伴肺动脉高压手术治疗

为提高小婴儿室缺伴肺动脉高压的手术疗效，总结１６例８ｋｇ以下室间隔缺损（室缺）伴肺动脉高压婴儿行急诊手术修补室缺的经验。其中１４例在深低温停循环下进行，手术效果满意；术后死亡１例（６％）。作者认为，婴儿巨型室缺应尽早手术治疗为宜，深低温停循环方法尤为适合；术后强调持续保持病婴镇静及呼吸道通畅，防止肺高压危象的发生。     

A型肉毒毒素对离体豚鼠心脏的抑制作用

本实验用Langendorff法,在恒温、恒液压(灌流压)和供给足够氧气的条件下,对豚鼠(Cavin porcellus)离体心脏进行灌流,以心肌收缩力、心跳频率、心电图和冠脉流量为观察指标,对照观察A型肉毒毒素对心脏活动的影响。结果发现:A型肉毒毒素可使(1)心肌收缩力减弱;(2)心跳频率减慢,甚至停搏;(3)冠脉流量减少;(4)心电图有异常改变,显示对心脏起搏有阻遏现象.     

复方栀子冲剂栀子甙薄层扫描测定

应用薄层扫描法测定了复方栀子冲剂中栀子甙的含量，对实验条件，以正交试验法进行优选，确定了最佳方案，排除了干扰，得到了满意的效果。     

The electrophysiological effects of dicentrine on the conduction system of rabbit heart.

1. The electrophysiological effects of dicentrine, an aporphine alkaloid isolated from the root of Lindera megaphylla, were examined in the Langendorff perfused rabbit heart and rabbit isolated cardiac cells. 2. Standard electrophysiological characters were measured in the Langendorff perfused rabbit heart (control study) and after 5 min exposure to 1, 3 and 9 microM of dicentrine and during the subsequent recovery phase sequentially (n = 7). The same study protocols were performed in 0.5 to 4.5 microM quinidine (n = 7), 18 to 162 microM procainamide and N-acetylprocainamide (n = 7) for comparison. 3. The results showed that the spontaneously beating heart rate and the sinoatrial (SA) and atrioventricular nodal (AH) conduction time were not significantly affected by dicentrine but were significantly suppressed by the higher doses of quinidine (4.5 microM) and procainamide (162 microM). 4. The His-Purkinje conduction time was significantly increased by the higher dose of dicentrine, quinidine and procainamide. 5. The ventricular repolarization time and its effective refractory period were significantly increased by the higher dose of dicentrine and the other agents. 6. The effective refractory period of the atrium, AV node and His-Purkinje system were also significantly increased by dicentrine and the other agents. 7. A voltage clamp study revealed that the prolongation of atrial action potential duration by dicentrine (9 microM) was associated with a significant inhibition of the transient potassium outward current. As well as inhibition of the transient outward current, a significant inhibition of the sodium inward current by dicentrine was found.(ABSTRACT TRUNCATED AT 250 WORDS)     

Influence of Qingdai Compound Capsule on the expression of c-myc in psoriatic keratinocytes) on the expression of c-myc in psoriatic keratinocytes

In this immunohistochemical study, the authors detected the expression of c-myc in keratinocytes (ECK) from 30 psoriatics before and after Qingdai Compound Capsule (QDCC) treatment and 12 normal subjects for control. The results showed that the ECK of patients before treatment was significantly higher than that in the normal control group, and its level was correlated with histopathological changes of affected skin. After treatment the ECK was normalized. It is believed that QDCC could decrease the ECK in psoriatics and this effect was probably mediated by inhibiting c-myc expression. This study provided an experimental evidence for the application of QDCC in treating psoriasis.     

医技人员血源性疾病感染情况调查

医务人员的医院内感染逐渐被人们所关注，其中血源性疾病的感染更应引起大家的重视。为了了解医务人员中医师、检验人员的医院感染发生情况，笔者对某中心医院的医师和检验人员的2002年度体检结果进行了统计分析，现报告如下。     

手助腹腔镜肝部分切除术治疗原发性肝癌

目的 探讨手助腹腔镜肝部分切除术治疗原发性肝癌的方法和应用价值。方法 经临床筛选的第Ⅱ、Ⅲ、Ⅵ段或边缘型的原发性肝癌6例，在腹腔镜辅助下．应用手助器、超声刀、切割器等特殊器械进行腹腔镜下肝癌切除术。结果 6例患者均成功完成手术，包括肝Ⅱ、Ⅲ段切除，Ⅵ段切除及非规则性肝段切除术，手术时间80～130min，术后住院6～9d，手术出血150～700ml，无中转开腹手术，无并发症。术后随访6～15个月，2例分别于手术后3和4个月肝内复发，其余4例随访至今无复发。结论 在经过临床选择的病例中，手助腹腔镜下作肝部分切除术治疗原发性肝癌是安全、可行的手术方式。