乌体林斯对老年肺结核患者外周血中T淋巴细胞亚群改变的影响

老年肺结核患者细胞免疫功能低下,乌体林斯是一种多功能免疫增强剂,我们在抗痨治疗的基础上加用乌体林斯治疗,取得了较好的疗效,现报告如下。1资料和方法1.1一般资料选取2004年3月～2006年10月我院住院的未经抗痨治疗的老年肺结核患者68例,其中男41例,女27例,年龄61～69岁,随机     

内脂素在MIN6胰岛β细胞的表达及葡萄糖和棕榈酸对其表达的影响

Visfatin was expressed in rat anti mouse islets,as well as in MIN6 cells. The visfatin expression was affected by various concentrations of environmental glucose (5.5 and 33.3 mmoL/L) and palmitate(0.5 mmol/L). As compared with low-level glucose medium (5.5 mmol/L, 1.0±0.11) , visfatin expression increased in media with high glucose and palmitate (1.32 ±0. 18, 1. 33±0. 15,1.72±0.27, all P<0. 05). The result suggests that visfatin seems to be involved in the regulation of insulin secretion.     

计算机导航在骨科手术中的应用

目的将计算机导航技术应用于骨科手术,并对其进行初步分析。方法2004年2～12月,计算机导航系统辅助下共行134枚椎弓根螺钉固定,51枚股骨颈骨折空心钉内固定,58枚交锁髓内钉远端交锁螺钉固定。结果所有病例全部在导航下完成手术,术中明显减少C型臂X线机透视次数,未发生血管和神经损伤并发症。结论计算机导航手术安全有效,更有利于微创手术的开展。     

Primary amyloidosis of the urinary bladder.

Amyloidosis is a systemic disease that usually occurs in the gastrointestinal tract or in muscular or adipose tissue. Primary amyloidosis of the urinary bladder is a rare disease that can mimic bladder cancer on cystoscopic examination as well as in its clinical presentation of painless gross hematuria. This report describes a 49-year-old male with repeated painless gross hematuria, who underwent transurethral resection of a suspected bladder tumor. Pathologic examination revealed papillary urothelial hyperplasia with vascular ectasia and no signs of malignancy. Massive gross hematuria occurred 2.5 years later. Cystoscopy showed multiple papillary lesions with yellowish-brown submucosal plaques on the posterior bladder wall. A second transurethral tumor resection was performed and histologic examination revealed plasma cell infiltration and eosinophilic amorphous deposits in the subepithelial stroma and vascular wall. The deposits were positive for Congo red and apple-green birefringence under polarized light examination but negative for Masson's trichrome stain, indicating that they were not fibrotic in nature. Hence, the diagnosis of amyloidosis of the urinary bladder was confirmed. Screening for amyloidosis was negative in other organ systems and the patient has remained disease-free up to the last follow-up 4 years after the second transurethral resection. Amyloidosis should be considered in the differential diagnosis of patients with recurrent hematuria who have symptoms characteristic of bladder cancer but negative pathologic study for malignancy. Correct diagnosis relies on clinical alertness and the use of a special staining technique during pathologic examination.     

结节性硬化症的磁共振影像学表现

目的　分析结节性硬化症TSC的MRI表现。方法　 13例经临床证实的TSC病例 ,分析其头部MRI特征性改变。结果　MRI主要征象包括 :①室管膜下结节 :13例均发现病灶 ,T1WI发现 71个 ,T2 WI 39个 ,PDWI 5 6个 ;②皮层及皮层下结节 :11例有阳性发现 ,T2 WI和PDWI各发现 114个病灶 ,T1WI 72个 ;③脑白质异常信号 :5例发现白质病变 ,T2 WI和PDWI各发现 10个病灶 ,T1WI无明确显示 ;④室管膜下巨细胞星形细胞瘤 :仅 1例发现。结论　MRI对TSC的中枢神经系统改变敏感 ,是诊断此病的首选影像学检查方法 ,其中T1WI观察室管膜下结节敏感 ,T2 WI和PDWI观察皮层下及白质病变敏感     

CY消毒湿面巾消毒效果的实验研究

以LJ-强化戊二醛为主体制成的CY消毒液。能快速杀灭细菌,10min内可灭活HBsAg,在有效浓度范围内不会对人体产生危害,应用安全可靠。以其制成湿面巾擦拭手、碗、筷2min,可将实验菌及自然菌减少99％,其细菌残留数均<30个/cm~2。拭后纸上无细菌残留,其丢弃物不会污染环境。室温下(35～39℃)放置3个月的CY湿面巾,外观无改变,消毒效果稳定。     

单侧鼻前庭切口经蝶切除垂体腺瘤

对１６例垂体腺瘤采用单侧鼻前庭切口经蝶切除，效果满意，既可减少手术创伤，又缩短了手术距离，且避免了美容缺陷，是一种设计巧妙，较为实用的手术方法，尤其适用于生长激素腺瘤。     

应用羧化胶乳凝集试验诊断人、畜布鲁氏菌病的研究

应用以化学交联法制备的布鲁氏菌１６Ｍ抗原－羧化胶乳制剂的羧化胶乳凝集试验（ＬＡＴ）、试管凝集试验（ＳＡＴ）、虎红平板凝集试验（ＲＢＰＴ）以及酶联免疫吸附试验（ＥＬＩＳＡ）、半胱氨酸凝集试验（ＣＹＴ），对在四省布鲁氏菌病疫区收集的部分人、畜血清中布氏菌抗体进行对比检测。结果表明，ＬＡＴ较之ＲＢＰＴ具有更高的特异性；与ＳＡＴ相比，无论阳性或阴性符合率均较一致。另外，ＬＡＴ可检测血清中ＩｇＧ、ＩｇＭ两类布氏菌特异性抗体。因而本试验对人、畜布病的诊断具有特异、敏感和简便的优点，更宜在基层推广应用。     

人脑胶质瘤逃避免疫监视机制的研究

为研究胶质瘤逃避免疫监视的机制，检测了胶质瘤体外细胞系培养上清（SN）抗PHA－P刺激正常人及胶质瘤患者外周血淋巴细胞增殖的作用。证实了胶质瘤可自分泌免疫抑制因子。经抗TGFβ2单抗中和SN试验，TGFβ2免疫组化染色及Northern杂交进一步表明该因子主要成分为TGFβ2并对胶质瘤患者血浆皮质醇，生长激素及儿茶酚胺含量及细胞免疫功能进行检测，发现血浆去甲肾上腺素显著降低，其水平与CD8亚群％呈负相关，与CD4／CD8比值及淋巴细胞增殖率呈正相关。初步提示存在胶质瘤的情况下，神经－免疫调控紊乱，可能起着抑制性调节作用。     

人参皂甙Rg1对大鼠局灶性脑缺血脑组织神经干细胞增殖的影响

目的 观察人参皂甙Rg1对大鼠局灶性脑缺血脑组织神经干细胞增殖的影响,探讨其在脑保护及抗衰老作用中的可能机制.方法 取成年雄性Wistar大鼠,用线栓法制作大鼠大脑中动脉闭塞(MCAO)模型,采用脉冲标记法,经腹腔注射5′-溴脱氧尿苷(BrdU)标记处于增殖状态的神经干细胞,用免疫组织化学单标及双标免疫荧光技术观察人参皂甙Rg1对局灶性脑缺血后BrdU和巢蛋白(nestin)的免疫活性及nestin/BrdU双标阳性细胞的影响,镜下观察脑内神经干细胞的分布,并计数行定量分析.结果 局灶性脑缺血后大鼠室管膜下区及海马齿状回有nestin、BrdU及nestin/BrdU双标免疫阳性细胞分布.应用人参皂甙Rg1后,上述各部位阳性细胞数明显增多,与缺血组之间有显著性差异(P＜0.01).结论 人参皂甙Rg1具有促使神经干细胞增殖的能力,这可能是其神经保护及抗衰老作用的机制之一.