利多卡因胶浆在小儿尿道扩张术中的应用

[目的]探讨利多卡因胶浆用于小儿尿道扩张术的效果。[方法]选择2004年2月-2006年6月尿道下裂尿道成形术后尿道狭窄患儿50例，随机分为对照组（用常规尿道扩张方法）和实验组（尿道扩张前尿道内注入利多卡因胶浆）。观察患儿疼痛程度、尿道黏膜损伤及一次插管成功率。[结果]实验组疼痛轻，尿道黏膜损伤率低，一次插管成功率高。[结论]利多卡因胶浆应用于小儿尿道扩张术可减轻疼痛，避免尿道损伤。     

Floating culture of human nasal glandular cells]

OBJECTIVE: To establish a floating cultural model of human nasal glandular (HNG) cells. METHODS: HNG cells were cultured on floating collagen gels. RESULTS: Cultured HNG cells incubated with monoclonal antibody for cytokeratin stained positively. After two-week culture, the cells stained blue by AB-PAS staining. Transmission electron microscopy showed that the cells exhibited numerous microvilli on their surfaces and had numerous secretary granules. They were well polarized and interconnected by junctional complexes. Cultured HNG cells possessed typical characteristics of normal epithelium. CONCLUSION: The floating cultural model of HNG was successfully established.     

口腔颌面部亚性肿瘤尿激主其受体的基因表达和临床意义

目的：探讨ＵＰＡ及ＵＰＡＲ与口腔颌面部亚性肿瘤浸润、转移的关系。方法；应用ＣＤＮＡ＝ＭＲＮＡ交技术定量检测口腔颌面部恶性肿瘤ＵＰＡ和ＵＰＡＲＭＴＲＡＮ表达水平。结果：癌组织ＵＰＡ和ＵＰＡＲＭＲＮＡ含量高上应正常组织（Ｐ〈０．０１），伴有淋巴结转移癌组织ＵＰＡ和ＵＰＡＲ高于淋巴结转移者（Ｐ〈０．０１，Ｐ〈０．０５），ＵＰＡ、ＵＰＡＲ表达水平与淋巴关系密切（Ｒ＝０．４１Ｐ〈０．０１，Ｒ＝０．３１，Ｐ〈     

复健汤加减治疗阻塞性肺气肿７６例临床观察

采用复健汤加减治疗阻塞性肺气肿76例.结果临床控制15例,显效34例,好转21例,无效6例,总有效率92.1%.治疗后咳嗽、咯痰、喘息、哮鸣音等有不同程度的改善,肺功能FVC、FEV1、MBC、MMEF治疗前后比较有显著差异(P<     

中药熏蒸治疗颈椎病、腰椎病及骨折术后功能康复病人的护理

中药熏蒸疗法是一种通过电脑温控加热产生对人体无损伤的中药湿热因子,并且利用蒸汽中的各种中药成分的渗透和经穴调节作用于人体的一种自然疗法。它将药理作用和热力有机结合起来,经皮肤吸收药物以及湿热作用扩张血管,具有强筋壮骨、祛风除湿、活血化淤、通经活络、消炎止痛等     

人型支原体的分离培养和生长特性的研究

目的研究人型支原体(Mh)的生长曲线、菌落形态演变等生物学特性及临床分离方法。方法取临床妇科阴道标本58例,同时接种液体培养基与固体培养基。在固体培养基上挑单菌落放入液体培养基进行纯化。选一株纯化的Mh接种于液体培养基,每隔4 h看颜色变化测pH值,并取10μL液体滴种在固体培养基上,计数菌落得出生长曲线。对不同生长时间的菌落拍照。从生长曲线得知对数生长期,并可在此期间进行保种。结果妇科阴道标本临床分离的Mh阳性率为8.62%;明确了在固体培养基上Mh菌落的生长演变过程;得到了Mh特有的生长曲线,并由此推出Mh在液体中最大生长数量及其时间、繁殖一代所需的时间和对数生长期及液体生化反应的pH值颜色改变规律。结论Mh的分离诊断,应该以固体培养基为基础,显微镜下看到典型的Mh特征性“油煎蛋”菌落为准。在液体培养基中Mh的传代以及保种在40 h左右,即1/2对数生长期最好。     

儿童恶性胶质瘤VEGF表达与血管生成及细胞增殖的关系

研究血管内皮细胞生长因子在儿童恶性胶质瘤的表达及其与血管生成，细胞增殖的关系。方法免疫组化方法检测３３例儿童恶性胶质瘤组织中ＶＥＧＦ表达与微血管数、ＰＣＮＡ标记指数。结果３３例儿童恶性胶质瘤ＶＥＧＦ表达阳性２３例；     

Effect of acid hydrolysis time on amino acid determination in casein and processed cheeses with different fat content

This work deals with hydrolysis curves for 15 amino acids (aspartic acid, threonine, serine, glutamic acid, proline, glycine, alanine, valine, isoleucine, leucine, phenylalanine, tyrosine, histidine, lysine and arginine) in bovine casein and in model-processed cheeses with 30, 45 and 60% (w/w) fat in dry matter. The effect of a different fat content on hydrolysis curves of the amino acids was observed. The hydrolysis curves were obtained by means of nonlinear regression using 13 hydrolysis intervals (range, 0–144 h). The amino acid content in hydrolysates was measured using ion exchange chromatography. Correction factors were calculated with the aim of increasing the accuracy of the amino acid determinations. With the rising fat content in samples, the correction factors increased for the majority of amino acids observed. The highest correction factors were determined for threonine, serine and tyrosine.