显微定量法测定中成药中猪牙皂的含量

目的 :制定猪牙皂在脐风散与惊风散中的显微定量标准。方法 :用显微定量法 ,以猪牙皂特有的石细胞为显微特征物 ,对脐风散与惊风散中的猪牙皂进行测定。结果 :猪牙皂含量与每毫克特征数呈显著的线性正相关 ,回归方程为 y =61 .42 x -1 .1 0× 1 0 - 3(γ=1 .0 0 ,α=0 .0 5 )。结论 :显微定量法测定脐风散与惊风散中猪牙皂的含量是可行的 ,结果可靠     

舰船员慢性荨麻疹与马拉色菌相关性研究

OBJECTIVE: To investigate the association of Malassezia furfur with chronic urticaria in the crew members of ships. METHODS: A comparative mycological study of 126 crew members of ships with chronic urticaria and 45 normal control subjects was carried out. The 82 urticaria patients identified as positive for Malassezia furfur were divided into groups A and B to receive treatment with antihistaminics (group A) and antihistaminics combined with 2% ketoconazole shampoo(group A). RESULTS: The carrier rates of Malassezia furfur were significantly higher in urticaria patients than in the normal control subjects (P<0.01), but in view of the case ratios of the final cure or improvement, no significant difference was observed between the two groups by the end of the treatment courses (P>0.05). But 6 to 8 weeks from the end of the treatment course, better therapeutic effect was noted in group B (P<0.01), with higher rate of negative Malassezia furfur findings (P<0.01). CONCLUSION: Malassezia furfur may play an important role in the prevalence of chronic urticaria among the crew members, and anti-fungal treatment may produce better long-term therapeutic effect.     

用鸡胚卵黄囊分离斑点热群立克次体的研究

本文在用传统的实验动物感染法分离立克次体同时，并尝试用鸡胚卵黄囊培养法直接分离立克次体。结果：用前者未分离出立克次体，而用后者分别从来自北京昌平县的中华革蜱和来自黑龙江省虎林县的嗜群血蜱中各分离到1株立克次体。经鉴定，这两株立克次体属于斑点热群立克次体。结论：鸡胚卵黄囊培养法可以直接从现场节肢动物标本中分离病原体。     

自体皮源奇缺条件下瘢痕挛缩畸形的晚期临床修复

目的寻找对烧伤后畸形伴自体皮源奇缺患进行晚期修复的新方法。方法5例特重度、特大面积烧伤患治愈后伴较严重畸形，且自体皮源奇缺。采用柔软成熟的瘢痕皮肤作为修复的皮源，用扩张器扩张瘢痕皮肤后，切取中厚瘢痕皮片进行修复；不宜行瘢痕皮肤扩张术时，切取刃厚瘢痕皮片与异体脱细胞真皮基质组成复合皮进行修复。观察两种方法的疗效。结果所移植的扩张瘢痕皮片和复合皮均成活，功能和外形恢复良好，远期疗效近似正常自体中厚皮移植。结论采用不同厚度的瘢痕皮片修复烧伤畸形是可行的，可扩大自体皮源。此法对于皮源奇缺的瘢痕患尤其适用。     

自体造血干细胞移植治疗恶性血液病

为探讨恶性血液病的有效治疗方法,应用自体骨髓移植(ABMT)38例,自体外周血造血干细胞移植(ABSCT)13例,自体外周血造血干细胞与自体骨髓联合移植15例。治疗白血病54例,恶性淋巴瘤11例,多发性骨髓瘤1例。外周血造血干细胞采用化疗加多抗甲素或C—CSF动员。移植物采用微波、阿克拉霉素净化处理。结果:两组动员方案均有良好动员效果。ABSCT组及联合移植组造血功能恢复比ABMT组快(P<0.05),合并症少。66例中,45例仍呈持续缓解(CCR),中位CCR时间32(5～98)个月,复发21例。3年无病生存率及复发率分别为68.5％及26.1％。结果表明:自体造血干细胞移植是根治恶性血液病的有效手段。ABSCT及联合移植具有造血功能恢复快,合并症少等优点。微波和阿克拉霉素体外净化移植物是一种简便、有效的净化方法。     

近视儿童配戴离焦软性接触镜与角膜塑形镜的安全性和有效性的观察比较

  目的  对比观察近视儿童配戴离焦软性接触镜与角膜塑形镜控制近视的有效性和安全性。  方法   回顾性研究2018年1月至2020年9月我院就诊的儿童近视患者临床资料,共75例儿童纳入分析。离焦软镜组:日戴周边离焦软性接触镜的近视儿童30例（60眼）,男性10例,女性20例,年龄（9.9±1.00）岁,等效球镜度数（?2.78±0.70）D。角膜塑形镜组:配戴角膜塑形镜的近视儿童45例(90眼),女性30例,男性15例,年龄（9.67±1.11）岁,等效球镜度数（?2.67±0.71）D。两组患儿均已配戴12个月及以上框架眼镜后验配接触镜。随访12个月,对比分析两组近视儿童眼轴变化情况及角膜结膜并发症情况。  结果  两组近视儿童戴框架眼镜期间眼轴增加较快,OrthoK组配前的框架期眼轴增量（0.34±0.09） mm,DISC组配前的框架期眼轴增量（0.37±0.07） mm;换为接触镜后戴接触镜12个月后,OrthoK 组眼轴增量（0.18±0.04） mm;DISC组眼轴增量（0.19±0.05） mm,两组儿童更换近视防控方案后眼轴增量均降低,差异有统计学意义（P<0.05）。两种不同接触镜的眼轴长度增量接近,两者差异无统计学意义（P>0.05）。配戴两种接触镜12个月期间,慢性滤泡性结膜炎发生率角膜塑形镜组为11.20%,离焦软镜组为20.00%,其中离焦软镜组比例略高,但差异无统计学意义(P>0.05)。角膜染色1级发生率角膜塑形镜组为66.70%,离焦软镜组为56.70%;角膜染色2级发生率角膜塑形镜组为17.80%,离焦软镜组为16.70%,其中两种程度的角膜染色角膜塑形镜组比例均略高于离焦软镜,但差异无统计学意义(P>0.05)。接触镜乳头性结膜炎发生率角膜塑形镜组为2.20%,离焦软镜组为10.00%,离焦软镜发生率略高于角膜塑形镜,但差异无统计学意义(P>0.05)。  结论  离焦软镜与角膜塑形镜均可有效控制近视儿童眼轴长度的进展,其控制效果均优于框架眼镜,两种接触镜对眼轴控制效果接近。两种接触镜的角结膜并发症较少,安全性较高。     

右美托咪定调控Nrf2/HO-1通路对H2O2诱导心肌细胞氧化应激损伤的作用研究

目的 探讨右美托咪定调控核因子E2相关因子2(Nrf2)/血红素加氧酶1(HO-1)通路对过氧化氢(H₂O₂)诱导心肌细胞氧化应激损伤的作用。方法 体外培养大鼠H9C2心肌细胞,设置对照组、H₂O₂组、1μmol右美托咪定+H₂O₂组、5μmol右美托咪定+H₂O₂组、10μmol右美托咪定+H₂O₂组。CCK-8法检测各组H9C2细胞增殖情况;酶联免疫吸附试验(ELISA)检测各组H9C2细胞丙二醛(MDA)、超氧化物歧化酶(SOD)水平;实时荧光定量聚合酶链反应(q RT-PCR)检测各组H9C2细胞Nrf2、HO-1 mRNA相对表达量;Western blotting检测各组H9C2细胞Nrf2、HO-1蛋白相对表达量。结果 与对照组比较,H₂O₂组H9C2细胞存活率、SOD水平、Nrf2、HO-1 mRNA及蛋白相对...     

Genetic basis of tobacco smoking: strong association of a specific major histocompatibility complex haplotype on chromosome 6 with smoking behavior

The genetic basis for addiction to tobacco smoking--particularly that of the perception of olfactory stimuli that may be important in reinforcing smoking addiction--is largely unknown. A cluster of genes for olfactory receptors is in close proximity to the MHC region on chromosome 6. Polymorphisms of MHC class III genes (RCCX modules, TNFA promoter polymorphisms) were determined in 101 healthy subjects and 232 coronary artery disease (CAD) patients from Hungary with defined tobacco smoking habits. A highly significant association between ever smoking (past + current smokers) and a specific MHC haplotype was observed (odds ratios = 2.14-4.13; P-values = 0.012 to <0.001). This haplotype is characterized by the presence of C4A null alleles and a solitary short C4B gene linked to the TNF2 allele of the promoter for TNFA gene. This haplotype occurred more frequently in the ever smokers than in the never smokers [odds ratio: 4.97 (1.96-12.62); P = 0.001], and such associations were stronger in women (odds ratio = 13.6) than in men (odds ratio = 2.79). An independent study of complement C4 protein polymorphism and smoking habits in Icelandic subjects (n = 351) yielded similar and confirmative results. Considering the documented link between olfactory stimuli and smoking in females, and the presence of a cluster of odorant receptor genes close to the MHC class I region, our findings implicate a potential role of the MHC-linked olfactory receptor genes in the initiation of smoking.