Effect of thyroid hormone level on the expression of synaptotagmin Ⅰ in adult rat hippocampus

Objective To observe the effect of different thyroid hormone level on the expression of synaptotagmin Ⅰ(Syt Ⅰ) in adult rat hippocampus. Methods All 28 adult male SD rats were assigned randomly into hypothyroid, hyperthyroid and control group, hypothyroid group was established by daily intraperitoneal injections with propylthiou raci(PTU, 10.0 mg/kg body weight) for 6 weeks and hyperthyroid group with L-Thyroxine (L-T4, 0.5 mg/kg body weight) for 3 weeks. Radioimmunity method was used to assay the levels of serum T3 and T4, immunohistochemical S-P technology to assay the levels of Syt Ⅰ protein in hippoeampus CA1, CA3 and dentate gyrus (DG). The layers analyzed in the different subfields include the polymorphic cell layer(the stratum oriens, SO), pyramidal cell layer(PCL), stratum radiatum (SR), lacunosum-molecular layer (SLM) in CA1 and CA3, granular cell layer(GL) and molecular layer(ML) in DG. Results The levels of serum T3 and T4[(0.34±0.12), (41.03± 11.37)nmol/L]in the hypothyroid rats were significantly lower than those in the control group[(0.65±0.15), (55.20±10.68)nmol/L, P < 0.01 or < 0.05], and the positive granule of Syt Ⅰ was significantly lower in PCL and SR of CA1 and CA3, GL of DG. The average optical value responsible for Syt Ⅰ immunoreactivity was obviously reduced in SO(0.048±0.007), PCL(0.299±0.035), SR(0.042±0.007), SLM(0.038±0.006) of CA1, PCL(0.085± 0.019), SR(0.040±0.011), SLM (0.038±0.006) of CA3, GL (0.076±0.019) of DG than normal controls (0.068± 0.014, 0.376±0.053, 0.053±0.008,0.056±0.009,0.118±0.026,0.052±0.010,0.053±0.009,0.099±0.015; P< 0.01 or < 0.05). Serum T3 and T4 levels [(1.43±0.30), (157.18±19.95)nmol/L]of hyperthyroid rats were significantly higher than those of control group(P < 0.01). The value was reduced in PCL(0.322±0.050), SR(0.039±0.006), SLM (0.042±0.006) of CA1, PCL(0.098±0.034), SR(0.046±0.013), SLM(0.046±0.010) of CA3 and GL(0.085± 0.024), ML (0.042±0.009) of DG (P < 0.05 or < 0.01). Conclusion Adult-onset of hypothyroidism and hyperthyroidism can reversibly decrease the expression of Syt Ⅰ in CA1, CA3 and DG regions of hippocampus.     

Effect of thyroid hormone level on the expression of synaptotagmin Ⅰ in adult rat hippocampus

Objective To observe the effect of different thyroid hormone level on the expression of synaptotagmin Ⅰ(Syt Ⅰ) in adult rat hippocampus. Methods All 28 adult male SD rats were assigned randomly into hypothyroid, hyperthyroid and control group, hypothyroid group was established by daily intraperitoneal injections with propylthiou raci(PTU, 10.0 mg/kg body weight) for 6 weeks and hyperthyroid group with L-Thyroxine (L-T4, 0.5 mg/kg body weight) for 3 weeks. Radioimmunity method was used to assay the levels of serum T3 and T4, immunohistochemical S-P technology to assay the levels of Syt Ⅰ protein in hippoeampus CA1, CA3 and dentate gyrus (DG). The layers analyzed in the different subfields include the polymorphic cell layer(the stratum oriens, SO), pyramidal cell layer(PCL), stratum radiatum (SR), lacunosum-molecular layer (SLM) in CA1 and CA3, granular cell layer(GL) and molecular layer(ML) in DG. Results The levels of serum T3 and T4[(0.34±0.12), (41.03± 11.37)nmol/L]in the hypothyroid rats were significantly lower than those in the control group[(0.65±0.15), (55.20±10.68)nmol/L, P < 0.01 or < 0.05], and the positive granule of Syt Ⅰ was significantly lower in PCL and SR of CA1 and CA3, GL of DG. The average optical value responsible for Syt Ⅰ immunoreactivity was obviously reduced in SO(0.048±0.007), PCL(0.299±0.035), SR(0.042±0.007), SLM(0.038±0.006) of CA1, PCL(0.085± 0.019), SR(0.040±0.011), SLM (0.038±0.006) of CA3, GL (0.076±0.019) of DG than normal controls (0.068± 0.014, 0.376±0.053, 0.053±0.008,0.056±0.009,0.118±0.026,0.052±0.010,0.053±0.009,0.099±0.015; P< 0.01 or < 0.05). Serum T3 and T4 levels [(1.43±0.30), (157.18±19.95)nmol/L]of hyperthyroid rats were significantly higher than those of control group(P < 0.01). The value was reduced in PCL(0.322±0.050), SR(0.039±0.006), SLM (0.042±0.006) of CA1, PCL(0.098±0.034), SR(0.046±0.013), SLM(0.046±0.010) of CA3 and GL(0.085± 0.024), ML (0.042±0.009) of DG (P < 0.05 or < 0.01). Conclusion Adult-onset of hypothyroidism and hyperthyroidism can reversibly decrease the expression of Syt Ⅰ in CA1, CA3 and DG regions of hippocampus.     

临床医学专业来华留学生《组织学和胚胎学》全英文实验教学实践

随着我国大学国际化理念的不断深入,安徽医科大学按照国际化的学生培养模式的要求,力图着力营造国际化的学习氛围,自2011年起开始招收海外留学生。结合在《组织学和胚胎学》教学过程中的一些实践,探讨医学院校《组织学和胚胎学》课程开展医学留学生全英文教学的经验与体会。     

微量元素与男性不育研究进展

男性不育症是临床上较为常见而复杂的问题。目前,国内许多男性学专家从不同的角度对不育症进行了一系列的研究,但与微量元素之间关系的探讨和研究,则是近十几年才开展起来的工作。必需微量元素的缺乏或有害微量元素的积蓄,对男性生殖生理功能和内分泌调节活动均会产生极大的影响。本文就近年来国内、外关于男性不育者精浆中微量元素的研究综述如下。     

大鼠淋巴结内肥大细胞免疫组织化学异质性研究

目的观察大鼠淋巴结内肥大细胞的分布及免疫组织化学异质性.方法采用甲苯胺蓝染色和免疫组织化学方法.结果与甲苯胺蓝邻片比较,淋巴结内肥大细胞分别呈 P物质、血管活性肠肽和神经肽 Y免疫反应阳性,主要分布于髓质,细胞核为阴性反应.在髓索中可见神经肽 Y免疫反应性神经纤维,呈串珠状和线状,交织成网.结论大鼠淋巴结组织中肥大细胞含有 P物质、血管活性肠肽和神经肽 Y免疫反应物,淋巴结内有神经肽 Y能神经分布,表明神经肽可能通过神经-内分泌-免疫网络系统调节免疫器官的功能活动.     

大鼠、小鼠舌下腺内瘦素及其受体的表达和意义

目的对大鼠和小鼠舌下腺内瘦素及其受体进行定性和定位研究。方法分别取SD大鼠和昆明种小鼠舌下腺,进行HE染色和免疫组织化学染色。结果大鼠和小鼠舌下腺纹状管及小叶间导管上皮细胞呈瘦素及其受体免疫反应阳性,而腺泡细胞则为阴性。结论大鼠和小鼠舌下腺的导管上皮中含有瘦素及其受体,提示它们可能参与调节舌下腺的分泌活动。     

P物质,血管活性肠肽和神经肽Y在大鼠颌下腺分布…

采用免疫组织化学ＡＢＣ方法观察了Ｐ物质（ＳＰ）、血管活性肠肽（ＶＩＰ）和神经肽Ｙ（ＮＰＹ）等神经肽在大鼠颌下腺内的分布和共存关系结果显示，颌下腺内有ＳＰ能、ＶＩＰ能和ＮＰＹ能神经支配，纤维呈串珠状或线状，主要走行于腺泡、导管和血管周围。颌下腺内副交感神经节细胞均呈ＳＰ、ＶＩＰ和ＮＰＹ免疫组化反应阳性。提示这些神经肽共存于神经节细胞内，可能参与调节颌下腺复杂的分泌活动和有效的血液供应。     

含自杀基因酵母菌Fcy::Fur融合基因重组逆转录病毒表达载体的构建与鉴定

目的：构建并鉴定含自杀基因酵母菌Fcy：：Fur融合基因的重组逆转录病毒表达载体，拟将用于肿瘤的基因治疗研究。方法：设计一对PCR引物，在引物的5′端分别引入EcoRⅠ和BamHⅠ酶切位点。以质粒pORF5-Fcy：：Fur为模板，采用常规PCR方法扩增Fcy：：Fur融合基因，并将其分别克隆进测序载体PCS2^ 及原核表达载体pGEX-6p-1进一步进行核酸序列测定和原核诱导表达研究。用EcoRⅠ和BamHⅠ将Fcy：：Fur融合基因从测序载体上切出，按正确阅读框架克隆进pLXSN中，重组子鉴定采用PCR法和酶切消化。结果：序列测定表明，扩增的基因即为Fcy：：Fur序列．Fcy：：Fur融合基因在大肠杆菌中获得了融合表达；重组逆转录病毒表达载体经PCR及双酶切鉴定表明：Fcy：：Fur以正确方向插入到pLXSN中。结论：成功构建含有自杀基因Fcy：：Fur的逆转录病毒表达载体，为进一步进行肿瘤实验性基因治疗奠定基础。     

扬子鳄消化管壁内粘膜下神经丛的形态学观察

消化管神经丛的分布及其生理活动的研究甚多,但对低等脊椎动物的研究报道较少.扬子鳄是我国特有的世界珍稀动物,本文用Karnovsky-Roots乙酰胆碱酯酸(AChE)组化法研究扬子鳄消化管粘膜下神经丛的分布,为肠神经丛的比较解剖学研究提供一些形态学资料.1 材料和方法 扬子鳄2条(雌雄各1),2%戊巴比妥钠10～15ml腔内注射麻醉,打开胸腹腔,从左心室灌注生理盐水,继而灌流4%多聚甲醛固定,取食管下段、胃体、十二指肠、空肠、回肠和大肠,置4%多聚甲醛固定6h,继入合20%蔗糖的0.1mol·L~(-1)PB液中,4℃过夜.制备厚25μm的冷冻切片.以亚铁氰化铜法显示AChE,孵育液内加四异丙基焦磷酰胺(iso-OMPA)非特异性胆碱酯酶抑制剂和免去底物为对照.     

Effect of thyroid hormone level on the expression of synaptotagmin Ⅰ in adult rat hippocampus

Objective To observe the effect of different thyroid hormone level on the expression of synaptotagmin Ⅰ(Syt Ⅰ) in adult rat hippocampus. Methods All 28 adult male SD rats were assigned randomly into hypothyroid, hyperthyroid and control group, hypothyroid group was established by daily intraperitoneal injections with propylthiou raci(PTU, 10.0 mg/kg body weight) for 6 weeks and hyperthyroid group with L-Thyroxine (L-T4, 0.5 mg/kg body weight) for 3 weeks. Radioimmunity method was used to assay the levels of serum T3 and T4, immunohistochemical S-P technology to assay the levels of Syt Ⅰ protein in hippoeampus CA1, CA3 and dentate gyrus (DG). The layers analyzed in the different subfields include the polymorphic cell layer(the stratum oriens, SO), pyramidal cell layer(PCL), stratum radiatum (SR), lacunosum-molecular layer (SLM) in CA1 and CA3, granular cell layer(GL) and molecular layer(ML) in DG. Results The levels of serum T3 and T4[(0.34±0.12), (41.03± 11.37)nmol/L]in the hypothyroid rats were significantly lower than those in the control group[(0.65±0.15), (55.20±10.68)nmol/L, P < 0.01 or < 0.05], and the positive granule of Syt Ⅰ was significantly lower in PCL and SR of CA1 and CA3, GL of DG. The average optical value responsible for Syt Ⅰ immunoreactivity was obviously reduced in SO(0.048±0.007), PCL(0.299±0.035), SR(0.042±0.007), SLM(0.038±0.006) of CA1, PCL(0.085± 0.019), SR(0.040±0.011), SLM (0.038±0.006) of CA3, GL (0.076±0.019) of DG than normal controls (0.068± 0.014, 0.376±0.053, 0.053±0.008,0.056±0.009,0.118±0.026,0.052±0.010,0.053±0.009,0.099±0.015; P< 0.01 or < 0.05). Serum T3 and T4 levels [(1.43±0.30), (157.18±19.95)nmol/L]of hyperthyroid rats were significantly higher than those of control group(P < 0.01). The value was reduced in PCL(0.322±0.050), SR(0.039±0.006), SLM (0.042±0.006) of CA1, PCL(0.098±0.034), SR(0.046±0.013), SLM(0.046±0.010) of CA3 and GL(0.085± 0.024), ML (0.042±0.009) of DG (P < 0.05 or < 0.01). Conclusion Adult-onset of hypothyroidism and hyperthyroidism can reversibly decrease the expression of Syt Ⅰ in CA1, CA3 and DG regions of hippocampus.