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Objective To investigate the effects of tissue specific cytosine deaminase/5-fluorocytosine (CD/5-FC) thermotherapy on hepatic metastasis of colonic carcinoma in nude mice. Methods Forty-five nude mice were randomly divided into control group, 5-FC group and 5-FC thermotherapy group according to the random number table (15 mice in each group). Mice models of hepatic metastasis of colonic carcinoma were established by portal vein injection of LoVo/CEACD cells. The hepatic metastasis rate and number of metastatic nodules of the 3 groups were compared by ehi-square test and one-way ANOVA. The pathological changes in tumor tissues and apoptotic index of tumor cells were observed. The expression of the CD gene in tumor tissues was detected by fluorescent quantitative RT-PCR and Western blot. Results The number of metastatic nodules and liver metas-tasis rate were 4.6±1.3 and 100.0% in control group, 2.2±1.0 and 60.0% in 5-FC group, 0.5±0.8 and 13.3% in 5-FC thermotherapy group, with statistical difference among the 3 groups (F=25.898, χ2=5.208, 19.548, 5.168, P<0.05). The mean apoptotic indexes of tumor cells of the 3 groups were 4.6%, 9.9% and 17.4%, respectively. Vacuolar degeneration, cell necrosis, cytolysis and apoptotic bodies were mostly observed in the 5-FC thermotherapy group. The expression of CD gene in tumor tissue was detected in all the groups. Conclusion Tissue specific CD/5-FC thermotherapy has inhibitory effects on the hepatic metastasis of LoVo cells transfected with CD gene. 相似文献
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Objective To investigate the effects of tissue specific cytosine deaminase/5-fluorocytosine (CD/5-FC) thermotherapy on hepatic metastasis of colonic carcinoma in nude mice. Methods Forty-five nude mice were randomly divided into control group, 5-FC group and 5-FC thermotherapy group according to the random number table (15 mice in each group). Mice models of hepatic metastasis of colonic carcinoma were established by portal vein injection of LoVo/CEACD cells. The hepatic metastasis rate and number of metastatic nodules of the 3 groups were compared by ehi-square test and one-way ANOVA. The pathological changes in tumor tissues and apoptotic index of tumor cells were observed. The expression of the CD gene in tumor tissues was detected by fluorescent quantitative RT-PCR and Western blot. Results The number of metastatic nodules and liver metas-tasis rate were 4.6±1.3 and 100.0% in control group, 2.2±1.0 and 60.0% in 5-FC group, 0.5±0.8 and 13.3% in 5-FC thermotherapy group, with statistical difference among the 3 groups (F=25.898, χ2=5.208, 19.548, 5.168, P<0.05). The mean apoptotic indexes of tumor cells of the 3 groups were 4.6%, 9.9% and 17.4%, respectively. Vacuolar degeneration, cell necrosis, cytolysis and apoptotic bodies were mostly observed in the 5-FC thermotherapy group. The expression of CD gene in tumor tissue was detected in all the groups. Conclusion Tissue specific CD/5-FC thermotherapy has inhibitory effects on the hepatic metastasis of LoVo cells transfected with CD gene. 相似文献
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Objective To investigate the effects of tissue specific cytosine deaminase/5-fluorocytosine (CD/5-FC) thermotherapy on hepatic metastasis of colonic carcinoma in nude mice. Methods Forty-five nude mice were randomly divided into control group, 5-FC group and 5-FC thermotherapy group according to the random number table (15 mice in each group). Mice models of hepatic metastasis of colonic carcinoma were established by portal vein injection of LoVo/CEACD cells. The hepatic metastasis rate and number of metastatic nodules of the 3 groups were compared by ehi-square test and one-way ANOVA. The pathological changes in tumor tissues and apoptotic index of tumor cells were observed. The expression of the CD gene in tumor tissues was detected by fluorescent quantitative RT-PCR and Western blot. Results The number of metastatic nodules and liver metas-tasis rate were 4.6±1.3 and 100.0% in control group, 2.2±1.0 and 60.0% in 5-FC group, 0.5±0.8 and 13.3% in 5-FC thermotherapy group, with statistical difference among the 3 groups (F=25.898, χ2=5.208, 19.548, 5.168, P<0.05). The mean apoptotic indexes of tumor cells of the 3 groups were 4.6%, 9.9% and 17.4%, respectively. Vacuolar degeneration, cell necrosis, cytolysis and apoptotic bodies were mostly observed in the 5-FC thermotherapy group. The expression of CD gene in tumor tissue was detected in all the groups. Conclusion Tissue specific CD/5-FC thermotherapy has inhibitory effects on the hepatic metastasis of LoVo cells transfected with CD gene. 相似文献
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热化疗对结肠癌LOVO细胞胞嘧啶脱氨酶基因表达的影响 总被引:11,自引:0,他引:11
目的 探讨温热疗法对转染胞嘧啶脱氨酶基因的基因表达的影响.方法 脂质体法将质粒G1CEACDNa转染结肠癌LOVO细胞,G418筛选抗体克隆并扩增,给予前药5-氟胞嘧啶(5-FC)进行热化疗;以β-actin为内参照用实时荧光定量PCR检测热化疗前后CD基因表达量的变化.结果 实时荧光定量PCR(Real-Time RT-PCR)检测得到热化疗前CD基因表达的CT值29.5300±1.1974,B.actinct值20.8200±2.7385;热化疗后CD基因表达的CT值30.285±1.201,β-actinet值21.225±2.237;行ΔΔCt法进行相对定量比较差异无统计学意义(P>0.05,t=1.4521,v=38).结论 温热疗法对转染G1CEACDNa的LOVO细胞CD基因的表达无明显影响. 相似文献
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裸鼠结肠癌肝转移模型的建立 总被引:8,自引:0,他引:8
目的 探讨建立裸鼠结肠癌肝转移模型的方法.方法 45只裸鼠随机分为三组,分别用门静脉注射法,脾脏注射法和肝脏注射法建立结肠癌肝转移模型,并比较三种肝转移瘤模型在结肠癌肝脏转移率和自然生存期.结果 门静脉注射组,脾脏注射组和肝脏注射组肝脏转移率分别为100%(15/15),60%(9/15),100%(15/15);自然生存期分别为41.47±2.35 d,042.69±2.13 d,41.31±2.55 d.结论 门静脉注射法建立裸鼠结肠癌肝转移模型是一种简便、有效的方法. 相似文献
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目的:探讨温热疗法对转染胞嘧啶脱氨酶基因的基因表达有无影响。方法:脂质体法将质粒GICEACDNa转染结肠癌LOVO细胞,G418筛选抗体克隆并扩增,给予前药5-氟胞嘧啶(5-FC)进行热化疗;以beta—actin为内参照用实时荧光定量PCR检测热化疗前后CD基因表达量有无变化。结果:实时荧光定量PCR(Real-TimeRT—PCR)检测得到热化疗前CD基因表达的ct值29.53土1.1974,beta—actinct值20.82±2.7385;热化疗后CD基因表达的ct值30.285±1.201,beta—actinct值21.225±2.237;行△△Ct法进行相对定量比较无明显差异(P〉0.05,t=1.4521,v=38)。结论:温热疗法对转染G1CEACDNa的LOVO细胞CD基因的表达无明显影响。 相似文献