基于数据挖掘的云南燥证证候规律研究

目的：总结云南燥证的证候规律,为下一步研究燥证病因病机奠定基础。方法：充分利用中医文献学信息资源,选择云南地区具有代表性的论著与医案,回顾性地进行燥证症状与证候信息检索整理,选取频数较高症状,应用聚类分析找出云南燥证的证候群,再运用主成分分析确定主要证型。以明确云南燥证的证候特征与分布规律。在此基础上,运用中医理论知识阐释统计结果,从而归纳总结出燥证的常见证型及证候特点。结果：云南燥证常见证型为燥邪犯肺证、肝火犯肺证、血燥证及肝郁脾虚证。结论：云南燥证的证候具有外燥与内燥并存,干燥与湿阻共见,常兼肝脾症状等特点。     

LASIK后弥漫性板层角膜炎的临床分析

Objective To study the causes,clinical features and prevention measures of diffuse lamel-lar keratitis (DLK)after LASIK. Methods Eleven patients ( 17 eyes) with DLK after LASIK among 199 pa-floats (398 eyes) from March 2007 to December 2007 were selected as observation group,and after the strict checks on operating room environment,pressure sterilizer,surgical instrument disinfection,preoperative con-junctival sac clean and corneal epithelium protection,5 patients (7 eyes) with DLK after LASIK among 148 patients (296 eyes) from December 2007 to May 2008 were selected as control group. Each stage's complex,clinical features and treatment process of DLK were analyzed. Results The incidence rate of DLK in obser-vation group was 4.27%,among which,stage Ⅰ was 3.01%,stage Ⅱ was 0.5% and stage Ⅳ (3 eyes) was 0.75%. The incidence rate of DLK in control group was 2.36%,among which,stage Ⅰ was 1.68% and stage Ⅱ was 0.67%. DLK of stage Ⅰ and stage Ⅱ happened 1～3 days after LASIK,it absorbed entirely after 7～10 days'treatment, and vision rocovered to normal. DLK of stage Ⅳ happened 3～7 days after LASIK, relatively white dense opacity under the lamellar of central cornea and flap melt could be seen through slit lamp. High hyperme-tropia was left after healing. Hypermetropia decreased partly after 3～6 months, and vision recovered to 0.6～1.0. Conclusion The occurrence of DLK after LASIK is related with multifactors. DLK can be prevented by the strict checks on each link of the operation and cured better if it is treated in time.     

LASIK后弥漫性板层角膜炎的临床分析

Objective To study the causes,clinical features and prevention measures of diffuse lamel-lar keratitis (DLK)after LASIK. Methods Eleven patients ( 17 eyes) with DLK after LASIK among 199 pa-floats (398 eyes) from March 2007 to December 2007 were selected as observation group,and after the strict checks on operating room environment,pressure sterilizer,surgical instrument disinfection,preoperative con-junctival sac clean and corneal epithelium protection,5 patients (7 eyes) with DLK after LASIK among 148 patients (296 eyes) from December 2007 to May 2008 were selected as control group. Each stage's complex,clinical features and treatment process of DLK were analyzed. Results The incidence rate of DLK in obser-vation group was 4.27%,among which,stage Ⅰ was 3.01%,stage Ⅱ was 0.5% and stage Ⅳ (3 eyes) was 0.75%. The incidence rate of DLK in control group was 2.36%,among which,stage Ⅰ was 1.68% and stage Ⅱ was 0.67%. DLK of stage Ⅰ and stage Ⅱ happened 1～3 days after LASIK,it absorbed entirely after 7～10 days'treatment, and vision rocovered to normal. DLK of stage Ⅳ happened 3～7 days after LASIK, relatively white dense opacity under the lamellar of central cornea and flap melt could be seen through slit lamp. High hyperme-tropia was left after healing. Hypermetropia decreased partly after 3～6 months, and vision recovered to 0.6～1.0. Conclusion The occurrence of DLK after LASIK is related with multifactors. DLK can be prevented by the strict checks on each link of the operation and cured better if it is treated in time.     

麦粒灸配合三棱针挑刺治疗扁平疣的疗效观察

目的观察麦粒灸配合三棱针挑刺治疗扁平疣的临床疗效。方法将104例扁平疣患者随机分为治疗组53例与对照组51例。治疗组采用麦粒灸配合三棱针挑刺治疗,对照组采用外涂维A酸乳膏治疗。比较两组近期(治疗后)和远期(治疗后3个月)的疗效。结果治疗组治疗后总有效率和痊愈率分别为90.2%和62.7%,对照组分别为86.0%和48.0%,两组比较差异具有统计学意义(P0.05)。治疗组治疗后3个月总有效率和痊愈率分别为92.2%和64.7%,对照组分别为86.0%和48.0%,两组比较差异具有统计学意义(P0.05)。结论麦粒灸配合三棱针挑刺是一种治疗扁平疣的有效方法。     

壳聚糖膜在眼表碱烧伤早期应用的实验研究

 目的 了解壳聚糖膜用于早期眼表碱烧伤预防睑球粘连的效果,为临床应用提供依据.方法 家兔15只,采用自身对照方法,右眼为实验组,左眼为对照组,分别制作眼表碱烧伤模型,实验组于结膜囊中放置壳聚糖膜,对照组不用.术后8周对术区行大体观察及组织学光镜观察.结果 实验组及对照组均发生严重睑球粘连,在组织学上未见明显差异,实验组15例标本均发生角膜穿孔,眼内容物脱出,对照组角膜呈白色混浊,外有纤维组织包裹,未见角膜溃疡、穿孔.结论 对于严重眼表化学烧伤,不能早期应用抗粘连药物,以免发生角膜穿孔,待角膜稳定后才可以应用壳聚糖膜.     

大肠杆菌在巨噬细胞中的降解与交叉递呈研究

目的 通过表达的红色荧光蛋白(DsRed)与卯白蛋白(ovalbumin,OVA)表位融合蛋白,研究表达此融合蛋白的大肠杆菌在巨噬细胞中降解与交叉递呈的时相.方法 构建红色荧光蛋白与卵白蛋白表位融合蛋白的原核表达质粒(pET-16bOR),流式细胞计数仪检测表达融合蛋白的大肠杆菌,然后动态观察此大肠杆菌在巨噬细胞中的降解与交叉递呈.结果 表达红色荧光蛋白(DsRed)与卵白蛋白(ovalbumin,OVA)表位融合蛋白的大肠杆菌能被488 nm波长激发荧光.在巨噬细胞中,内质网与吞噬的大肠杆菌共聚;吞噬的大肠杆菌3～5 h快速降解;而在此时相产生的H-2Kb-OVA257-264复合物达到顶峰.结论 表达此红荧光蛋白与OVA表位融合蛋白的大肠杆菌具有荧光特性,内质网参与大肠杆菌吞噬体形成,巨噬细胞吞噬大肠杆菌后3～5 h是抗原快速降解和交叉递呈的时相.     

机体微环境失调在原发性胆汁性胆管炎发病机制中的作用

原发性胆汁性胆管炎（PBC）是一种慢性进展性自身免疫病,其主要病理表现为肝内胆汁淤积,胆管上皮细胞炎症损伤及肝内中、小胆管的持续性炎症损伤,最终导致肝细胞发生不可逆损伤。PBC的发病机制包括遗传、汇管区炎症损伤、黏膜免疫失调和胆管上皮细胞功能改变,肝脏和机体整体的微环境失调是诱发肝纤维化和肝硬化的始动因素。该文就机体代谢微环境、炎症反应微环境和免疫微环境失调在PBC发病机制中作用的研究进展作一综述。     

LASIK后弥漫性板层角膜炎的临床分析

Objective To study the causes,clinical features and prevention measures of diffuse lamel-lar keratitis (DLK)after LASIK. Methods Eleven patients ( 17 eyes) with DLK after LASIK among 199 pa-floats (398 eyes) from March 2007 to December 2007 were selected as observation group,and after the strict checks on operating room environment,pressure sterilizer,surgical instrument disinfection,preoperative con-junctival sac clean and corneal epithelium protection,5 patients (7 eyes) with DLK after LASIK among 148 patients (296 eyes) from December 2007 to May 2008 were selected as control group. Each stage's complex,clinical features and treatment process of DLK were analyzed. Results The incidence rate of DLK in obser-vation group was 4.27%,among which,stage Ⅰ was 3.01%,stage Ⅱ was 0.5% and stage Ⅳ (3 eyes) was 0.75%. The incidence rate of DLK in control group was 2.36%,among which,stage Ⅰ was 1.68% and stage Ⅱ was 0.67%. DLK of stage Ⅰ and stage Ⅱ happened 1～3 days after LASIK,it absorbed entirely after 7～10 days'treatment, and vision rocovered to normal. DLK of stage Ⅳ happened 3～7 days after LASIK, relatively white dense opacity under the lamellar of central cornea and flap melt could be seen through slit lamp. High hyperme-tropia was left after healing. Hypermetropia decreased partly after 3～6 months, and vision recovered to 0.6～1.0. Conclusion The occurrence of DLK after LASIK is related with multifactors. DLK can be prevented by the strict checks on each link of the operation and cured better if it is treated in time.     

壮腰健肾丸引起过敏反应1例报道

1病例报告患者,女,59岁,2003年9月1日19时因腰痛服用壮腰健肾丸1粒(江西侨业药业有限公司生产,剂量:5.6g/丸,批号:030112),服药约20min后,开始出现皮肤搔痒、唇舌肿大、头晕、呕吐、眼睛视物不清、呼吸困难等症状,后患者昏迷,即送入上犹县中医院治疗。入院时体格检查:患者呼吸急促、双眼睑充血、两肺呼吸音粗、未闻及干湿罗音、心率增快、四肢皮肤可见皮疹。T36.5℃、P120次/min、BP70/50kPa,初步诊断:过敏反应。给予静脉滴注5%葡萄糖注射液加维生素C、ATP、辅酶A、欣维、克林霉素静滴、扑尔敏10mg肌注、地塞米松10mg肌注,连续治疗3d,患…     

小牛血去蛋白提取物滴眼液治疗中重度干眼的效果研究

目的:探究中重度干眼患者接受小牛血去蛋白提取物滴眼液治疗的价值.方法:选取中重度干眼患者100例,选取时间为2015年8月22日-2016年10月25日,根据其治疗顺序的不同进行分组,其中实验组接受小牛血去蛋白提取物滴眼液治疗,对照组则接受玻璃酸钠滴眼液治疗,对2组中重度干眼患者治疗的结果进行观察分析.结果:实验组中重度干眼患者治疗的总有效率(98.00%)明显高于对照组干眼患者的总有效率(80.00%),P<0.05;2组中重度干眼患者治疗后的Schirmer试验、BUT结果均较治疗前有所提高,其中实验组干眼患者的Schirmer试验、BUT结果提高程度优于对照组(P<0.05).结论:中重度干眼患者接受小牛血去蛋白提取物滴眼液治疗,可以较好促进患者症状的缓解,意义重大.