中山医科大学隆重举行梁伯强教授诞辰一百周年纪念活动

梁伯强教授是我国著名的病理学家和医学教育家 ,中科院首批院士 (学部委员 )。 1999年 11月 4日在中山医科大学讲学厅隆重举行梁伯强教授诞辰一百周年纪念活动暨病理学术研讨会。参加这次纪念活动的来宾 130余人 ,包括梁伯强教授早年的学生 5 0余人 ,其中有钟世镇、程天民、姚开泰、甄永苏 4位院士。佘铭鹏教授、吴秉铨教授应邀出席 ;广州地区病理界同仁、梁伯强教授的夫人及亲属和黄洁夫校长等出席了这次的纪念活动。陈汝筑副校长主持了大会 ,大家在会上纷纷发言 ,缅怀一代宗师梁伯强教授一生严谨治学、锲而不舍、精益求精、为发展我国病理…     

EXPRESSION OF TISSUE-TYPE PLASMINOGEN ACTIVATOR IN SMOOTH MUSCLE CELLS OF INJURED ILIAC ARTERIES IN RABBITS

In this experiment, expression of tissue-type plasminogen activator (t-PA) in smooth muscle cells(SMCs) was measured at different iutervals after the arterial injury. In the normal lilac arteries, only low levels of t-PA activity were estimated, t-PA activity in extracts of the iliac arteries increased significantly at the 4th day after the injury, equivalent to the process that SMCs migrated from the media to the intima,and the t-PA activity was then decreased approximately to the normal level at the 7th day. Coexistent to the above data, results from in situ hybridization showed that the expression of t-PA mRNA in the intimaas well as media increased also significantly nr the 4th day after the arterial injury, and at the 7th day, t-PA mRNA was detected only in those SMCs locating closely adjacent to the internal elastic lamina. These results suggest that t-PA might play an important role in SMC migration following endothelial injury, and antagcaaism of t-PA expression and/or activity within the vessel wall might be helpful in intervening the devnlopment of restenosis following angioplasty.     

CHARACTERIZATION OF HDL BINDING SITES AND ITS LIGAND IN CULTURED SMOOTH MUSCLE CELLS OF RABBIT''S AORTA

Cultured smooth muscle cells of rabbit aorta were studied by 125I labelled rabbit HDL3. Saturation curves, measured at 4 C, showed the presence of two different components: the low-affinity non-saturable binding portion and the high-affinity binding portion (Kd about 5.6 x 10(-8) mol/L and Bmax about 0.321 micrograms/mg cell protein). Scatchard analyses of the high-affinity binding portion suggest the presence of single class binding sites. Binding of rabbit HDL3 to cultured smooth muscle cells was relatively resistant to trypsin or pronase, and independent to Ca2+. The binding rate of 125I-HDL3 to the smooth muscle cells was highest at 4 C and the optimal pH was 2. Additionally, presence of high concentration apoAI reduced 50% of the binding rate of 125I-HDL3, and 125I-HDL3, being pretreated (blocked) with rat anti-rabbit apoAI IgG of different concentrations lost 70% of its original binding rate with smooth muscle cells. The results suggest that rabbit aorta smooth muscle cells possess a specific binding sites for apoE-free HDL which recognizes apoAI as a ligand.     

中华医学会病理学分会第7次大会

中华医学会病理学分会第7次大会自2000年6月5～8日在大连召开。本次会议共收到论文近650篇,到会约500人。大会交流论文14篇,专题会报告280篇,文摘选登195篇,刊登文章标题80篇,外科病理讨论25例。中华医学会副     

高脂血症家兔体内不同部位巨噬细胞载脂蛋白E mRNA表达的研究

高脂血症家兔体内不同部位巨噬细胞载脂蛋白ＥｍＲＮＡ表达的研究王宗立，许漫，段宏伟，张颖，赵三妹，夏人仪，佘铭鹏（中国医学科学院基础医学研究所病理研究室，北京１００００５）高脂血症时除单核细胞进入动脉内膜吞噬脂质外，全身各部位的巨噬细胞均可发生脂质沉积...     

脂蛋白代谢紊乱的遗传基础

脂蛋白代谢紊乱的遗传基础赵庆伟佘铭鹏（中国医学科学院基础医学研究所、中国协和医科大学基础医学院，北京１００００５）ＡｂｓｔｒａｃｔＬｉｐｏｐｒｏｔｅｉｎｄｉｓｏｒｄｅｒｓｒｅｓｕｌｔｆｒｏｍａｂｎｏｒｍａｌｓｙｎｔｈｅｓｉｓ，ｐｒｏｃｅｓｓｉｎｇ，ｏ...     

Advances in the pathogenesis of atherosclerosis.

ＡｄｖａｎｃｅｓｉｎｔｈｅｐａｔｈｏｇｅｎｅｓｉｓｏｆａｔｈｅｒｏｓｃｌｅｒｏｓｉｓＳｈｅＭｉｎｇｐｅｎｇ佘铭鹏，ａｎｄＹｕＬｉｑｉｎｇ余立清Ｉｎｒｅｃｅｎｔ１０ｙｅａｒｓ，ａｌｏｎｇｗｉｔｈｔｈｅｒａｐｉｄｐｒｏｇｒｅｓｓｏｆｍｏｌｅｃｕｌａｒｃｙ...     

树鼩血清高密度脂蛋白和低密度脂蛋白物理化学性质的研究

本文用电子显微镜观察了树鼩高密度脂蛋白(HDL)和低密度脂蛋白(LDL)的形态和颗粒大小;用分光光度计和园二色仪研究了HDL和LDL的光谱特性和园二色性;用四甲基脲电泳、SDS-凝胶电泳和聚焦电泳分离了HDL中载脂蛋白的种类和含量。其结果如下: 一、电子显微镜观察HDL、LDL的形态和颗粒大小:结果证明,树鼩血清HDL和HDL均呈园球形,大小比较均匀。HDL的平均直径约为98A,80％以上的直径在80A—110A之间。统计结果表明,LDL的颗粒直径为196A,其中直径在160A—220A的颗粒约占84％。这表明LDL比较均一。 二、树鼩血清HDL的分析超速离心行为:结果表明,树鼩血清LDL和HDL在分析超速离心分析时,只呈现一个单一的     

Interaction of alcohol and the G to a substitution at the promoter region of the apolipoprotein AI gene in determining plasma apolipoprotein AI levels in Yi and Han Chinese

Ｌｏｗｐｌａｓｍａｈｉｇｈｄｅｎｓｉｔｙｌｉｐｏｐｒｏｔｅｉｎｃｈｏｌｅｓｔｅｒｏｌ(ＨＤＬＣ)ａｎｄｉｔｓｃｏｎｓｔｉｔｕｅｎｔａｐｏｌｉｐｏｐｒｏｔｅｉｎｓ(ａｐｏＡＩａｎｄａｐｏＡＩＩ)ｌｅｖｅｌｓｈａｖｅｂｅｅｎｆｏｕｎｄｔｏｂｅｉｎｄｅｐｅｎｄｅｎｔｒｉｓｋｆａｃｔｏｒｓｆｏｒｃｏｒｏｎａｒｙｈｅａｒｔｄｉｓｅａｓｅ(ＣＨＤ)ｉｎｓｅｖｅｒａｌｓｔｕｄｉｅｓａｎｄｈｉｇｈｅｒｃｏｎｃｅｎｔｒａｔｉｏｎｓｏｆＨＤＬＣａｎｄｔｈｅｃｏｎｓｔｉｔｕｅｎｔａｐｏｌｉｐｏｐｒｏｔｅｉｎｓｏｆｆｅｒｐｒ…     

去纤酶和tPA对血管平滑机细胞迁移和增生的影响

血管平滑肌细胞(SMC)的迁移和增殖是引起经皮冠脉腔内成形术(PTCA)后再狭窄的关键因素之一。组织型纤溶酶原激活物(tPA)是纤溶系统的主要成份之一,用于冠心病等的溶栓治疗,效果较好,但其对动脉SMC迁移和增殖的作用及机制和临床意义研究尚少。tPA在正常和罹病血管的分布仍大部分未被揭示,血管损伤后tPA的改变和局部SMC增殖和激活状态间的关系仍未被详细揭示,研究结果也不一致。tPA与高血脂对SMC增殖的作用有何相互影响,尚未见报道。去纤酶是我国首次从主产于我国的尖吻蝮蛇蛇毒中分离、纯化并第一通过国家鉴定的蛇毒制剂,由于其去纤抗凝作用,用于血管闭塞性疾病等的治疗。但其对血管SMC增殖的作用及其机理研究尚少,其与tPA作用