肾炎康复片联合前列地尔治疗糖尿病肾病的临床研究

目的 观察肾炎康复片联合前列地尔治疗糖尿病肾病的临床效果.方法 使用随机数字表法将武装警察部队总医院收治的81例糖尿病肾病患者分为对照组（40例）和观察组（41例）,严格控制2组患者血脂及血糖.对照组给予前列地尔10 μg,1次/d;观察组给予前列地尔10 μg,1次/d,肾炎康复片5粒/次,3次/d.观察2组患者临床疗效以及治疗前后的总胆固醇、三酰甘油、尿素氮、肌酐、24 h尿蛋白含量、舒张压和收缩压.结果 观察组总有效率为92.7％ （38/41）,明显高于对照组的80.0％（32/40）,差异有统计学意义（P＜0.05）;2组患者治疗后的总胆固醇、三酰甘油、收缩压、舒张压、尿素氮、肌酐和24 h尿蛋白与治疗前比较,差异均有统计学意义[观察组：（2.9 ± 0.4） mmol/L比（5.5±0.7） mmol/L、（1.7±0.2） mmol/L比（3.6 ±0.7） mmol/L、（81±6）mmHg（1 mmHg =0.133 kPa）比（89 ±7）mmhg、（133±8）mmHg比（145±10）mmHg、（8.2±1.9） mmol/L比（13.2±2.0）mmol/L、（199±61） μmol/L比（259±88） μmol/L、（1.1 ±0.4）g/24 h比（2.5±0.6）g/24 h;对照组：（3.9±0.4）mmol/L比（5.6±0.7）mmol/L、（2.4±0.4） mmol/L比（3.6±0.7） mmoL/L、（85±6） mmhg比（89±6）mmhg、（135±8）mmhg比（144±9） mmhg、（10.8±1.6） mmol/L比（13.3±2.2） mmol/L、（202±63） μmol/L比（264±91） μmol/L、（1.6±0.5）g/24 h比（2.4±0.5）g/24 h]（均P＜0.05）.观察组治疗后尿素氮、24 h尿蛋白、总胆固醇和三酰甘油水平明显低于对照组,差异均有统计学意义（均P＜0.05）.结论 前列地尔联合肾炎康复片治疗早期糖尿病肾病的临床总有效率较高,能够明显改善肾功能,延缓糖尿病肾病的进展.     

日本血吸虫重组抗原的免疫原性鉴定

为发展新的日本血吸虫病疫苗候选抗原分子，对已构建的阳性表达克隆PGsj24进行大量诱导表达，制备为20kD的重组抗原。以之免疫家兔，产生了较强的抗体反应。该单特导抗血清可识别成上天然蛋白中与重组蛋白相对应的抗原成分。两者分子量及Westernblot识别反应带型均基本一致，说明约20kD重组蛋白确为日本血吸虫基因编码产物，具有较强的免疫原性，可刺激机体产生较强的免疫应答。     

Activation of extrasynaptic GABAA receptors inhibits cyclothiazide-induced epileptiform activity in hippocampal CA1 neurons

Extrasynaptic GABA_A receptors (GABA_ARs)-mediated tonic inhibition is reported to involve in the pathogenesis of epilepsy. In this study, we used cyclothiazide (CTZ)-induced in vitro brain slice seizure model to explore the effect of selective activation of extrasynaptic GABA_ARs by 4,5,6,7-tetrahydroisoxazolo[5,4-c] pyridine-3-ol (THIP) on the CTZ-induced epileptiform activity in hippocampal neurons. Perfusion with CTZ dose-dependently induced multiple epileptiform peaks of evoked population spikes (PSs) in CA1 pyramidal neurons, and treatment with THIP (5 μmol/L) significantly reduced the multiple PS peaks induced by CTZ stimulation. Western blot showed that the δ-subunit of the GABA_AR, an extrasynaptic specific GABA_AR subunit, was also significantly down-regulated in the cell membrane 2 h after CTZ treatment. Our results suggest that the CTZ-induced epileptiform activity in hippocampal CA1 neurons is suppressed by the activation of extrasynaptic GABA_ARs, and further support the hypothesis that tonic inhibition mediated by extrasynaptic GABA_ARs plays a prominent role in seizure generation.     

安氏Ⅰ类错病人正畸治疗前后咬合接触特征的变化

目的观察安氏 I类青少年错病人在矫治前后的咬合接触特征的变化情况,并探讨其临床指导意义。方法选择 2013年 5月至 2016年 3月就诊于北京民航总医院口腔正畸门诊 16例安氏 I类青少年错颌畸形病人,于全口 28颗牙粘托槽进行不拔牙矫治,使用 T?ScanⅢ咬合分析仪记录受试者正畸治疗前后的咬合接触特征,并进行比较。结果正畸治疗后 16例病人前牙力百分比平均值减小(8.33±3.85)%(P＜0.05)后牙力百分比增加(8.33±15.13)%(P＜0.05)力中心点 COF(Cen? ter of Force,力中心点距上切牙唇面的垂直距离)向位置后移(4.88±9.86)mm(P＜0.05),左侧矢状,分离时间减少(0.32± 0.77)s(P＜0.05)右侧分离时间减少(0.19±0.20)s(P＜0.05),咬合接触时间与前伸分离时间差异无统计学意义(P＞ 0.05);力分布度变化不明显;仍有 4例病人存在平衡,干扰。结论青少年错病人在规范的矫治治疗后咬合状况会有明显改善,但需注意力分布与咬合干扰,消除咬合隐患后再予拆除矫治器。     

血清降钙素原和C-反应蛋白在细菌感染性疾病诊断中的临床价值

目的探讨血清降钙素原和C-反应蛋白在细菌感染性疾病诊断中的临床价值。方法收集2012年5月,2013年12月期间我院收治的83例细菌感染性疾病患者并将其作为研究组,收集同期我院收治的83例非细菌感染性疾病患者并将其作为对照组,采用双抗夹心免疫发光法检测血清PCT水平,免疫比浊法对CRP水平进行测定,比较PCT、CRP测定结果,并评价两种筛选试验结果。结果研究组与对照组比较,血清PCT、CRP水平、PCT阳性检测率均明显较高,差异有统计学意义（P〈0．05）,PCT检测与CRP检测比较,诊断灵敏度、特异度、阳性预测值、阴性预测值、诊断符合率均较高,差异有统计学意义（P〈0．05）。结论PCT与CRP比较对细菌感染性疾病的诊断敏感性更高,实施血清PCT水平的监测对细菌感染性疾病的诊疗具有重要的临床价值。     

Multicenter study of the clinical features and mutation gene spectrum of Chinese children with Dent disease

Dent disease is a rare X-linked recessive inherited tubular disease. In this multicenter study, the clinical presentation and genetic background of Chinese children with Dent disease are studied to improve the cognition and diagnostic ability of pediatricians. In this prospective cohort, we described the genotype and phenotype of a national cohort composed of 45 pediatric probands with Dent disease belonging to 45 families from 12 different regions of China recruited from 2014 to 2018 by building up the multicenter registration system. The CLCN5 gene from 32 affected families revealed 28 different mutations. The OCRL gene from 13 affected families revealed 13 different mutations. The incidence of low-molecular-weight proteinuria (LMWP) in both Dent disease type 1 populations and Dent disease type 2 populations was 100.0%; however, the incidence of other manifestations was not high, which was similar to previously reported data. Therefore, LMWP is a key clinical feature that should alert clinicians to the possibility of Dent disease. A high amount of LMWP combined with positive gene test results can be used as the diagnostic criteria for this disease. The diagnostic criteria are helpful in reducing the missed diagnosis of this disease and are beneficial for protecting the renal function of these patients through early diagnosis and early intervention.     

脑卒中患者延续性护理质量评价指标体系的构建

目的 构建脑卒中患者延续性护理质量评价指标体系,以期为评价脑卒中患者延续性护理质量提供全面、系统、可量化的标准。方法 在文献研究、半结构式访谈和小组讨论的基础上拟订指标体系初稿,并编制函询问卷,采用德尔菲法对全国17名专家进行2轮函询,运用层次分析法确定各级指标的权重。 结果 2轮专家函询问卷的有效回收率分别为100.0%、94.1%,专家权威系数分别为0.930、0.935,肯德尔和谐系数分别为0.451、0.409,P<0.01。最终形成的脑卒中患者延续性护理质量评价指标体系包括4个一级指标（信息的延续性、管理的延续性、关系的延续性、患者的结局）、15个二级指标、56个三级指标。 结论 构建的脑卒中患者延续性护理质量评价指标体系具有较好的科学性与可靠性,内容全面,对规范脑卒中患者延续性护理具有指导意义。     

[99mTc]MIBI SPECT/CT for Identifying Dystonic Muscles in Patients with Primary Cervical Dystonia

Molecular Imaging and Biology - This study aimed to evaluate the usefulness of [99mTc]sestamibi ([99mTc]MIBI) single photon emission computed tomography (SPECT)/X-ray computed tomography (CT)...     

Catalytic performance and deactivation of Ni/MCM-41 catalyst in the hydrogenation of pure acetylene to ethylene

Ni/MCM-41 catalysts were prepared by an impregnation method for acetylene hydrogenation to ethylene based on the calcium carbide acetylene route. X-ray diffraction and transmission electron microscopy indicated that Ni was uniformly dispersed on the support. Temperature-programmed reduction and X-ray photoelectron spectroscopy demonstrated a strong interaction between Ni and MCM-41, and Ni(0) and Ni(ii) coexisted in the catalyst. We optimized the catalytic activity by optimizing the Ni loading and reaction conditions including temperature, space velocity, and hydrogen/acetylene ratio. The acetylene conversion reached 100%, the ethylene selectivity reached 47%. Additionally, we tested the catalyst stability; the acetylene conversion was maintained at 100% for 25.73 h and was then rapidly reduced. ICP, TEM, FT-IR, thermogravimetric analysis and BET were used to investigate the reasons for catalyst deactivation; it was found that green oil deposition on the catalyst surface was the main reason for the catalyst deactivation.

Ni/MCM-41 catalysts were prepared by an impregnation method for acetylene hydrogenation to ethylene based on the calcium carbide acetylene route.     

预见性护理程序应用于脑出血患者的效果评价

目的 探讨预见性护理程序应用于脑出血的护理效果.方法 将120例脑出血的住院患者随机分为实施预见性护理程序组和常规护理组,对两组护理方式的效果进行比较研究.结果 常规护理组的平均住院日高于预见性护理程序组(P＜0.05),差异有显著性,并发症发生率和接入院通知至进行各项治疗护理操作开始所用时间,常规组均高于预见性护理程序组(P＜0.01).结论 实施预见性护理程序后患者更快地接受治疗及护理,减少并发症的发生,对脑出血患者的康复具有重要意义.