骨髓增生异常综合征及慢性原因不明性粒细胞减少症患者骨髓CFU—GM和CFU—F的初步观察

利用人类骨髓粒一单核系祖细胞集落(CFU—GM)和成纤维细胞集落(CFU—F)体外检测技术,比较观察32例骨髓增生异常综合征(MDS)和11例慢性原因不明性粒细胞减少症(CIN)患者的造血改变。结果表明,MDS 和 CIN 的造血状态存在显著区别,69%的 MDS 患者 CFU—GM集落显著受抑,集簇、簇/落比值明显升高;其 CFU—F 集落亦存在明显缺陷。而 CIN 患者的上述指标均类似于正常。这提示体外集落检测技术有助于 MDS 和 CIN 的鉴别。此外,CFU—GM 系列培养对 MDS 患者具有一定预后价直。     

袖珍式野战静脉麻醉控制器的研制

介绍一种采用单片机开发的按药代动力学参数控制注射泵注射给药,从而控制手术患者静脉麻醉深度,适合野战条件下使用的小型化静脉麻醉系统.该系统具有控制精度高、功能扩展能力强以及使用方便等特点,并已在临床推广应用.     

体外细胞培养检验“方证对应”关系的方法学研究

目的通过在体外细胞培养中加入中药复方粗提制剂对具有典型的中医证型的肺癌患者的外周血淋巴细胞NK活性的影响，探索能否在体外实验中反映“方证对应”关系。方法以水煎醇沉法制备两种中药复方（益气养阴和健脾化痰）的粗提制剂，取具有典型的中医证型（气阴两虚或脾虚痰湿证型）的肺癌患者的外周血淋巴细胞，将同一个患者的标本分为四个体外实验组，即空白对照组，中药5mg／ml组及10mg／ml组，自细胞介素2（250^u/ml，阳性对照）组。按“辨证论治”原则加入相应的中药复方制剂。体外培养22h或94h，以MTT法检测各组的NK活性。结果无论培养22h或94h，中药组的NK活性与对照组比较皆无显著性差异（P〉0．05），而白细胞介素2（阳性对照）组的NK括性均明显升高（P〈0．05）。结论两种中药复方制剂都未能在体外反应出“方证对应”的效果，说明中药粗提制剂直接用于体外细胞培养，其结果的可靠性差。     

Brain dysfunction in social drinkers

Thirty-four social drinkers who had referred themselves to the Regional Brain Damage Unit for assessment of the effects of drinking alcohol were compared with 42 volunteer control subjects of equivalent age but with low alcohol intake, using two 'learning' tests — the Rey Auditory Verbal Learning Test (RAVLT) and the Austin Button Maze. The Maze Test gave no evidence of disorder, but the two groups were significantly different on the RAVLT. No abnormalities in standard cognitive tests were apparent. These results suggest that a deficit in learning ability may be an early feature of the brain dysfunction associated with excessive alcohol consumption.     

维克胃炎冲剂治疗慢性浅表性胃炎44例临床观察

将胃镜确诊的慢性浅表性胃炎８４例分为两组，治疗组４４例，男性２８例，女性１６例、平均年龄３３±１６岁。口服维克胃炎冲剂１包（５ｇ３次／ｄ）疗程为２０ｄ，显效率７９．５％。对照组４０例：男性２６例，女性１４例，平均年龄３６±１３岁，口服三九胃泰１包３次／ｄ，疗程为２０ｄ，显效率为２５％，两组比较Ｐ＜０．０１，值得选用维克胃炎冲剂。     

Clinicopathologic features and incidence of invasive lobular carcinoma in Japanese women

Intending to clarify the true Incidence of Invasive lobular carcinoma of the breast In Japanese women as well as the frequency of unilateral multlcentriclty, 362 cases of clinically defined monocentrlc breast cancer without pre-operative biopsy (previously fine needle aspiration or needle biopsy were routinely carried out for every case) were examined by whole mammary gland serial sectioning. On the basis of pathology and the World Health Organization classification of breast tumors, each case was assigned to one of two main histologlc types: Invasive lobular carcinoma (ILC) or Invasive ductal carcinoma (IDC). Invasive lobular carcinoma was further separated into classic and variant types by employing previously published criteria. Twenty-one cases of ILC (5.8%) were diagnosed, which Is more than In most previous Japanese studies. Unilateral multicentric breast carcinoma was detected In 9.5% of ILC and 16.1% of IDC (the difference was found not significant). Microscopically, ILC tumors were found to be, on average, larger than IDC. Patients with classic type ILC tended to be younger than those with variant type or IDC. Estrogen receptor expression was found more frequently In variant type ILC than in classic type. These results suggest that the incidence of invasive lobular carcinoma of the breast In Japanese women is low and that unilateral multicentricity Is not significantly higher in ILC than in IDC.     

补肾中药佳蓉片对去势小鼠骨丢失的预防作用

目的　研究补肾中药佳蓉片 (JRP)对模型小鼠骨质疏松的影响 .方法　雌性昆明小鼠 6 0只随机分为 6组 ,行去卵巢术或假手术 .处理后分组为 :去卵巢组 (OVX) ,假手术组(Sham) ,去卵巢分为佳蓉片低剂量组 (JRP- L,10 0 mg·d- 1 ) ,佳蓉片中剂量组 (JRP- M,2 0 0 mg· d- 1 ) ,佳蓉片高剂量组 (JRP- H,40 0 mg· d- 1 )和 17β雌二醇组 (E2 ,10 μg·d- 1 ) .手术后灌胃上述药物 2 8d,测定股骨密度、骨生物力学性能、血清碱性磷酸酶 (AL P)、骨钙素 (BGP) .结果　各中药组的股骨骨密度均高于去卵巢对照组 (P<0 .0 5 ) ,与假手术组无差异 ;灌胃中药后骨结构力学参数显著性改善 (P<0 .0 5 ) ,同时 AL P,BGP显著性降低 (P<0 .0 5 ) .结论　补肾中药方剂可预防去卵巢引起的骨丢失 ,改善骨的生物力学性能     

Neural basis of implicit motor sequence learning: Modulation of cortical power

Implicit sequence learning describes the acquisition of serially ordered movements and sequentially structured cognitive information, that occurs without awareness. Theta, alpha and beta cortical oscillations are present during implicit motor sequence learning, but their role in this process is unclear. The current study addressed this gap in the literature. A total of 50 healthy adults aged between 19 and 37 years participated in the study. Implicit motor sequence learning was examined using the Serial Reaction Time task where participants unknowingly repeat a sequence of finger movements in response to a visual stimulus. Sequence learning was examined by comparing reaction times and oscillatory power between sequence trials and a set of control trials comprising random stimulus presentations. Electroencephalography was recorded as participants completed the task. Analyses of the behavioral data revealed participants learnt the sequence. Analyses of oscillatory activity, using permutation testing, revealed sequence learning was associated with a decrease in theta band (4–7 Hz) power recorded over frontal and central electrode sites. Sequence learning effects were not observed in the alpha (7–12 Hz) or beta bands (12–20 Hz). Even though alpha and beta power modulations have long been associated with executing a motor response, it seems theta power is a correlate of sequence learning in the manual domain. Theta power modulations on the serial reaction time task may reflect disengagement of attentional resources, either promoting or occurring as a consequence of implicit motor sequence learning     

Unilateral Multicentric Breast Carcinoma Studied by Whole Mammary Gland Serial Sectioning

The incidence and clinicopathologic features of unilateral multicentric breast cancer (UMBC) were studied by mammary gland serial sectioning in 116 cases of clinically defined monocentric breast cancer (MONBC) examined histopathologically at the Nagano Cancer Detection Center. UMBC was defined as: 1) histopathologically discontinuous tumors each with an intraductal spread, 2) at least one tumor-free section separating two tumors, and 3) a large primary tumor and other small secondary tumors. UMBC was detected in 23 of 116 cases (19.8%), all with one secondary tumor. Primary and secondary tumors were located in the same quadrant in 34.8% and in different ones in 65.2%. The secondary tumors were <5 mm in size in 56.5%. Secondary tumors, averaging 8.3 mm in size and 25.5 mm in distance from the primary tumor, were almost exclusively noninvasive carcinomas, including 15 (65.4%) noninvasive ductal carcinomas and several special types. The primary and secondary tumors were of the same histologic type in 3 of 23 cases. UMBC patients averaged 6 years younger than MONBC patients, and the incidence of UMBC tended to be higher in younger patients (p<0.1). UMBC tended to occur more frequently in quadrant with an average histologie tumor size significantly smaller than that in MONBC (p<0.01). The histologie types of the primary tumor in UMBC and MONBC were similar, with common types predominant. Lymph node metastases tended to be slightly more frequent in MONBC. This high incidence of UMBC calls for careful attention when considering breast conserving therapy.     

新生儿严重脑室内出血连续腰穿的疗效

目的为了治疗和预防脑室内出血后脑积水的发生和发展,改善新生儿严重脑室内出血的不良预后。方法1989年以来,对19例在出生后早期经头颅B超诊断为严重脑室内出血（IVH）患儿进行了连续腰穿（LP）治疗。结果有效16例,自动出院1例,失败2例。有效率为84．2％。有效16例初次LP日龄为14．6±5．7d,疗程23．4±12d,平均LP次数9．3±4．7次,平均LP间隔期3．1±1．6d,平均每次放液量6．7±2ml,LP治疗后起效天数为10．1±7．8d。2例在连续LP的同时联用乙酰唑胺治疗亦取得显著疗效。16例平均随访月龄为3．2±1月,体格发育均正常,B超显示12例脑室形态正常,4例脑室呈稳定的轻度增大。失败的2例初次LP迟,初期LP间隔期延长为7－9d,放液量＜5ml。同期另有17例严重IVH患儿未予LP治疗。其中4例并发脑积水,5例自动出院,4例早期死亡,余4例其后脑室形态稳定。结论连续LP不失为治疗新生儿严重IVH实用安全有效的方法,LP疗效欠佳时可联用乙酰唑胺治疗。