中药口服与灌肠配合氦-氖激光治疗老年人慢性肾功能衰竭临床观察

观察调中益肾活血通腑中药口服与灌肠配合低能量氦 -氖激光血管内照射(ILIB)疗法对老年人慢性肾功能衰竭 (CRF)的疗效。治疗组予中药口服与灌肠配合ILIB疗法 ,并与阳性对照药依那普利作比较。结果 :两组病例在Scr、BUN、尿蛋白方面均有显著降低 ,但治疗组在改善临床症状、提高红细胞SOD及血浆免疫球蛋白方面均优于对照组。结论 :调中益肾活血通腑中药口服与灌肠配合ILIB疗法具有明显改善老年CRF的肾功能、调整胃肠机能、提高机体免疫能力的作用。     

“治未病”在中医妇科临床中的应用

结合文献与临床研究,总结何嘉琳教授在中医妇科临床工作中运用“治未病”理论的学术思想与临床用药经验。     

Magnetic resonance elastography of the lung: technical feasibility.

Magnetic resonance elastography (MRE) is a phase-contrast technique that can spatially map shear stiffness within tissue-like materials. To date, however, MRE of the lung has been too technically challenging-primarily because of signal-to-noise ratio (SNR) limitations and phase instability. We describe an approach in which shear wave propagation is not encoded into the phase of the MR signal of a material, but rather from the signal arising from a polarized noble gas encapsulated within. To determine the feasibility of the approach, three experiments were performed. First, to establish whether shear wave propagation within lung parenchyma can be visualized with phase-contrast MR techniques, MRE was performed on excised porcine lungs inflated with room air. Second, a phantom consisting of open-cell foam filled with thermally polarized (3)He gas was imaged with MRE to determine whether shear wave propagation can be encoded by the gas. Third, preliminary evidence of the feasibility of MRE in vivo was obtained by using a longitudinal driver on the chest of a normal volunteer to generate shear waves in the lung. The results suggest that MRE in combination with hyperpolarized noble gases is potentially useful for noninvasively assessing the regional elastic properties of lung parenchyma, and merits further investigation.     

Comparison of airway diameter measurements from an anthropomorphic airway tree phantom using hyperpolarized 3He MRI and high-resolution computed tomography.

An anthropomorphic airway tree phantom was imaged with both hyperpolarized (HP) 3He MRI using a dynamic projection scan and computed tomography (CT). Airway diameter measurements from the HP 3He MR images obtained using a newly developed model-based algorithm were compared against their corresponding CT values quantified with a well-established method. Of the 45 airway segments that could be evaluated with CT, only 14 airway segments (31%) could be evaluated using HP 3He MRI. No airway segments smaller than approximately 4 mm in diameter and distal to the fourth generation were adequate for analysis in MRI. For the 14 airway segments measured, only two airway segments yielded a non-equivalent comparison between the two imaging modalities, while eight more had inconclusive comparison results, leaving only four airway segments (29%) that satisfied the designed equivalence criteria. Some of the potential problems in airway diameter quantification described in the formulation of the model-based algorithm were observed in this study. These results suggest that dynamic projection HP 3He MRI may have limited utility for measuring airway segment diameters, particularly those of the central airways.     

3He MRI in mouse models of asthma.

In the study of asthma, a vital role is played by mouse models, because knockout or transgenic methods can be used to alter disease pathways and identify therapeutic targets that affect lung function. Assessment of lung function in rodents by available methods is insensitive because these techniques lack regional specificity. A more sensitive method for evaluating lung function in human asthma patients uses hyperpolarized (HP) (3)He MRI before and after bronchoconstriction induced by methacholine (MCh). We now report the ability to perform such (3)He imaging of MCh response in mice, where voxels must be approximately 3000 times smaller than in humans and (3)He diffusion becomes an impediment to resolving the airways. We show three-dimensional (3D) images that reveal airway structure down to the fifth branching and visualize ventilation at a resolution of 125 x 125 x 1000 microm(3). Images of ovalbumin (OVA)-sensitized mice acquired after MCh show both airway closure and ventilation loss. To also observe the MCh response in naive mice, we developed a non-slice-selective 2D protocol with 187 x 187 microm(2) resolution that was fast enough to record the MCh response and recovery with 12-s temporal resolution. The extension of (3)He MRI to mouse models should make it a valuable translational tool in asthma research.     

郃郎喘必消冲剂的药理研究

本文通过对小鼠急性毒性实验,抗炎实验,对胸腺脾脏的影响及对豚鼠在体、离体气管平滑肌作用实验,观察了郃郎喘必消冲剂的药理学效应。结果表明,该制剂无明显毒性,最大耐受量为1000g/kg/d。它有明显的对抗二甲苯致小鼠耳炎及延长组胺诱发哮喘发作潜伏期的作用,对豚鼠离体气管平滑肌无松驰作用,对小鼠胸腺和脾脏有使其重量减小的趋势,但与对照组比较无显著性差异。     

Kubelka-Munk模型下的兔血管对氦-氖激光的散射与吸收特性

测量了兔动脉和静脉对 He-Ne激光的反射和透射传输特性。实验采用两积分球系统及波长为632.8nm的He-Ne激光器,并根据测量数据及采用 Kubelka-Munk模型分析和计算了兔动脉和静脉组织对该长激光的吸收系数、散射系数及总的光强I（x）及前向散射通量i(x）和后向散射通量j(x）随厚度的变化情况。结果表明,兔动脉和静脉的漫反射率和透射率有明显差别,而且,动脉对激光的吸收系数明显较静脉的要小,而动脉对激光的散射系数却明显较静脉的要大,在动脉和静脉组织中总的光强I（x）及前向散射通量i(x）和后向散射通量j(x）随组织厚度的变化情况也有明显的区别。     

He-Ne激光重复照射抑制培养增生性瘢痕成纤维细胞胶原合成的实验研究

目的 探讨Ｈｅ－Ｎｅ激光重复照射对培养增生性瘢痕成纤维细胞胶原合成的抑制作用。方法 以不同功率密度（１０、５０、１００和１５０ｍＷ／ｃｍ＾２）Ｈｅ－Ｎｅ激光照射培养增生性瘢痕成纤维细胞３０ｍｉｎ,１／ｄ,连续照射３ｄ,在３ｄ重复照射后２４ｈ用＾３Ｈ－脯氨酸掺入法和斑点杂交法分别检测成纤维细胞胶原合成和Ⅰ型前胶原基因表达。结果 培养增生性瘢痕成纤维细胞的胶原合成、Ⅰ型前胶原基因表达水平在１０、５０ｍ     

中药配合低能量氦—氖激光血管内照射为主治疗缺血性中风疗效观察

目的：观察中药配合低能量氦－氖激光血管内照射为主治疗缺血性中风疗效。方法：治疗组：用中药配合低量氦－氖激光血管内照射为主治疗缺血性中风６９例;对照组：用降纤酶、尼莫通治疗缺血性中风４２ 例。结果：治疗组基本痊愈４８ 例,显效８例,有效７例,无效５ 例,恶化１ 例,有效率为９１．３０％ 。对照组基本痊愈２０例,显效１例,有效９ 例,无效１１ 例,恶化１例,有效率为７１．４３％ 。经χ２ 检验,χ２＝ ７．６,Ρ＜ ０．０１,有显著差异。结论：治疗组中药配合低能量氦－氖激光血管内照射为主治疗缺血性中风综合疗效明显优于对照组用降纤酶和尼莫通治疗缺血性中风     

无机陶瓷膜精制技术对半夏泻心颗粒定性鉴别的影响

目的：探讨无机陶瓷膜精制工艺对半夏泻心汤成分的影响，并建立半夏泻心颗粒的定性鉴别方法。方法：应用薄层色谱法对半夏泻心颗粒中主要成分半夏、干姜、黄芩等进行了定性鉴别及膜精制前后的比较。结果：样品经无机陶瓷膜精制后，薄层层析分离效果好，斑点清晰，特征性强。结论：无机陶瓷膜精制工艺可使方中各主要化学成分得到保留。