脑心通胶囊提取物通过增强CXCR4表达促进心脏干细胞迁移

[目的]探讨脑心通(NXT)对小鼠心脏干细胞(CSCs)CXCR4表达的影响。[方法]应用实时荧光定量聚合酶链式反应(PCR)芯片技术研究脑心通对心脏干细胞功能基因表达的影响。采用流式细胞术检测脑心通对心脏干细胞CXCR4表达的影响。应用蛋白免疫印迹法(Western Blot)检测脑心通对心脏干细胞CXCR4蛋白表达的影响。应用Transwell法测定脑心通对SDF-1诱导的心脏干细胞迁移能力的影响。[结果]芯片结果发现脑心通促进心脏干细胞CXCR4基因的表达;流式细胞技术结果显示,脑心通促进心脏干细胞APC-CXCR4阳性细胞率;Western Blot结果表明,脑心通促进心脏干细胞CXCR4蛋白表达;Transwell迁移实验结果显示,脑心通促进SDF-1诱导的心脏干细胞的迁移作用。[结论]脑心通可以促进心脏干细胞CXCR4的表达。     

Training nurses in contraceptive implant procedures: implications for practice in Australia

BackgroundThe contraceptive implant is a long-acting, effective method of contraception. Low uptake in Australia may be partially due to limited clinicians trained in implant procedures. Internationally, nurse-led implant procedures are accepted practice; however, this is not common in Australia.AimAn evaluation was undertaken to determine the effectiveness of implant training for nurses and consider the implications for clinical service delivery.MethodsParticipating nurses (n = 5) completed pre- and post-training surveys, and three were subsequently interviewed. Supervising doctors and nurses (n = 5) were also interviewed. A file audit was conducted to review implant procedures undertaken post-training.FindingsNurses undertook implant training to acquire new skills and meet patient demand. After the training, all nurses self-reported feeling ‘very confident’ in inserting the implant and at least ‘a little confident’ in removing the implant; the latter had minimal impact on removal success, as indicated in the file audit. Overall, nurses and supervising doctors and nurses felt that nurses could play a greater role in the provision of contraceptive implant procedures in Australia.DiscussionNurse-led procedures would increase access to the contraceptive implant for women, and have a positive impact on service delivery in different healthcare settings; however, funding constraints for nurses remain a significant barrier.ConclusionNurses are well placed to undertake contraceptive implant procedures. This would increase access to this method of contraception for women. These findings may inform models of care that promote nurse-led contraceptive procedures in Australia.     

脑心通胶囊联合西药治疗颈动脉粥样硬化的系统评价

目的:系统评价脑心通胶囊联合西药治疗颈动脉粥样硬化的临床疗效及安全性。方法:电子检索PubMed、Cochrane library、Embase、CNKI、VIP、万方和CBM,检索时间限定为自建库至2017年6月4日,检出所有脑心通胶囊联合西药治疗颈动脉粥样硬化的有关文献,2名研究员独立严格进行纳入研究的质量评价和资料提取,用Stata 14.0软件进行Meta分析,用TSA v 0.9软件进行试验序贯分析。结果:共纳入22个RCT包括2 484例患者。Meta分析结果显示IMT(WMD=-0.20,95%CI[-0.23,-0.16])、斑块积分(WMD=-0.57,95%CI[-0.72,-0.41])、TC(WMD=-0.66,95%CI[-0.82,-0.50])、TG(WMD=-0.48,95%CI[-0.73,-0.22])、LDL-C(WMD=-0.57,95%CI[-0.92,-0.23])、HDL-C(WMD=0.16,95%CI[0.10,0.21])、hs-CRP(WMD=-0.82,95%CI[-1.16,-0.48])比较,差异均有显著性,治疗组优于对照组;不良反应发生率(RR=1.477,95%CI[0.98,2.24])比较,差异无显著性,治疗组与对照组相当。试验序贯分析提示脑心通胶囊联合西药改善IMT疗效显著,证据确切。结论:脑心通胶囊联合西药能有效提高颈动脉粥样硬化的临床疗效,值得临床推广。     

中药脑心通对血管内皮细胞一氧化氮和内皮素-1的影响

目的 观察脑心通对氧化低密度脂蛋白(Ox-LDL)诱导的人脐静脉内皮细胞(HUVEC) 一氧化氮(NO)和内皮素-1(ET-1)分泌量的影响.方法 以培养HUVEC作为靶细胞,在内皮细胞培养基中加入Ox-LDL(50 mg/L)制备细胞损伤模型,并以不同浓度脑心通含药血清预先进行干预,分别采用硝酸还原酶法和放免法检测细胞上清液中的NO及ET-1的含量.结果 Ox-LDL作用HUVEC 24h后,HUVEC上清液中NO含量明显降低,而ET-1的含量明显升高,均明显高于正常对照组(P＜0.05);而预先加入脑心通含药血清或阿托伐他汀药液组NO含量明显升高,且在加入步长脑心通含药血清组,这种作用在一定剂量范围内随药物剂量的增加而增强(P＜0.05).结论 步长脑心通能够通过促进内皮细胞NO的合成与释放从而发挥对血管内皮细胞的保护作用,有利于减轻或抑制动脉粥样硬化的形成与发展.     

脑心通胶囊对Ⅱ型糖尿病小鼠伤口愈合的作用

[目的]研究脑心通胶囊(NaoXinTong,NXT)对Ⅱ型糖尿病小鼠伤口愈合的作用和作用机制。[方法]将8-12周db/+小鼠db/db小鼠适应性喂养1周后,按体质量随机分组db/+Saline组、db/db Saline、db/db NXT(0.7 g/kg)组,建立切除伤口夹板模型,每天灌胃给药并记录小鼠体质量,每两天监测空腹血糖并对伤口拍照测量伤口面积,分别在术后7、16 d取小鼠伤口处皮肤组织,小鼠伤口处皮肤组织进行切片或立即冷冻保存。HE染色观察伤口边缘肉芽组织的面积和上皮化,Lectin免疫荧光检测伤口周围毛细血管的密度,新鲜组织用来提取蛋白并用Western Blotting检测VEGF、PI3K/p-PI3K、AKT/p-AKT、eNOS/p-eNOS在伤口边缘的表达。[结果]与db/db Saline组相比,db/db NXT组小鼠伤口愈合率增加,肉芽组织面积和上皮化增加,伤口周围的血管密度和VEGF的表达增加,PI3K/AKT/eNOS的磷酸化增加,但在伤口愈合过程中糖尿病小鼠的体质量和空腹血糖没有变化。[结论]脑心通胶囊通过激活PI3K/AKT/eNOS信号通路增加VEGF表达促进血管新生,增加肉芽组织的形成和上皮化,加速Ⅱ型糖尿病小鼠伤口愈合。