妇女更年期心血管系统失调的护理

1妇女更年期心血管系统失调的表现1.1潮红潮红是血管舒张和收缩失调引起的一种症状。患者突感胸部压迫感，心悸，有时眼前发黑，然后感到一阵潮热，自下而上涌向头部，颜面潮红出汗。有的患者则怕冷或阵发性出汗而不伴随潮红。潮红发作的频率及延续时间因人而异。     

超临界CO2萃取灯盏花中总黄酮成分的工艺研究

目的：探讨从灯盏花中提取总黄酮成分的工艺。方法：采用超临界CO2萃取法，并与水提醇沉法比较。结果：超临界CO2萃取的最佳工艺为：压力为25MPa，夹带剂为85％乙醇，萃取温度为40％，萃取时间3h，夹带剂加入量800mL，CO2流量为15L／h。结论：从灯盏花中提取总黄酮成分，超临界CO2萃取是可行的。     

脑星形细胞瘤中TIP30、VEGF和CD34的表达及其相关性研究

Objective To investigate the expression and relationship of TIP30(HIV-1 Tat interactive protein 2), vascular endothelial growth factor (VEGF) and MVD (detected by CD34) in the angiogenesis of human brain astrocytomas. Methods Expression of TIP30, VEGF and CD34 in 19 cases of normal brain tissue and 71 cases of astrocytoma were immunohistochemically examined with Elivision plus two-step method. Results The positive expression of TIP30 could be seen in cytoplasm of neuroglial cells and neurons of 19 normal brain tissues. The positive expression rate of TIP30 in 71 cases of astrocytoma was 33.80 % (24/ 71). The positive expression rate of TIP30 in astrocytoma of different grades was 52 % for grade Ⅱ, 34.78 % for grade Ⅲ and 13.04 % for grade Ⅳ. The positive expression rate of TIP30 in high grade (Ⅲ+Ⅳ) of astrocytoma was found significantly lower than that in low grade(Ⅱ) (χ2=5.71, P <0.05); The expression of VEGF and MVD detected by CD34 in astrocytomas were higher than that in normal brain tissue and increased as the tumor grade increased; In astrocytoma, the negtive correlation was found between the expression of TIP30 and VEGF (r=-0.428, P<0.05); no correlation was found between TIP30 and MVD(r=-0.065, P 0.05); the positive correlation was found between VEGF and MVD(r=0.684, P<0.01). Conclusion The positive expression rate of TIP30 in normal brain tissue is significantly higher than that in astrocytoma. The positive expression rate of TIP30 significantly decreases as the pathological grade of the astrocytoma increases; The expression of TIP30 and VEGF is negatively correlated in astrocytoma.     

单次灌胃杂色曲霉素对小鼠大脑细胞的影响

目的　探讨单次ig杂色曲霉素 (ST)对小鼠大脑细胞的影响。方法　采用形态学观察和流式细胞术定量检测方法 ,研究ST对BALB/c小鼠大脑神经细胞的影响。结果　病理形态学观察可见 ,ig小剂量ST(3μg·kg-1 )后 1 2h或大剂量ST(3mg·kg-1 )后6h即可见小鼠大脑皮质、丘脑、海马CA2区神经元出现胞核固缩深染、胞浆嗜酸性变、空泡变性等病理变化 ,且随剂量增大和作用时间延长 ,病变神经元逐渐增多 ;光镜下对海马CA2区病变神经元进行计数分析 ,结果表明ST处理组发生病理变化的神经元比例均高于相应对照组 ,且呈剂量和时间依赖性增高 ;流式细胞术定量检测结果表明 ,igST 3,30 ,30 0和30 0 0 μg·kg-1 1 2h后 ,小鼠脑细胞的凋亡率呈剂量依赖性增高 ;ig3mg·kg-1 的ST后 6～48h,随ST作用时间的延长 ,脑细胞凋亡率也明显增高。结论经口给予ST可导致小鼠大脑皮质、丘脑、海马CA2区神经元发生退行性病变 ,诱导并促进小鼠大脑细胞凋亡     

脑星形细胞瘤中TIP30、VEGF和CD34的表达及其相关性研究

Objective To investigate the expression and relationship of TIP30(HIV-1 Tat interactive protein 2), vascular endothelial growth factor (VEGF) and MVD (detected by CD34) in the angiogenesis of human brain astrocytomas. Methods Expression of TIP30, VEGF and CD34 in 19 cases of normal brain tissue and 71 cases of astrocytoma were immunohistochemically examined with Elivision plus two-step method. Results The positive expression of TIP30 could be seen in cytoplasm of neuroglial cells and neurons of 19 normal brain tissues. The positive expression rate of TIP30 in 71 cases of astrocytoma was 33.80 % (24/ 71). The positive expression rate of TIP30 in astrocytoma of different grades was 52 % for grade Ⅱ, 34.78 % for grade Ⅲ and 13.04 % for grade Ⅳ. The positive expression rate of TIP30 in high grade (Ⅲ+Ⅳ) of astrocytoma was found significantly lower than that in low grade(Ⅱ) (χ2=5.71, P <0.05); The expression of VEGF and MVD detected by CD34 in astrocytomas were higher than that in normal brain tissue and increased as the tumor grade increased; In astrocytoma, the negtive correlation was found between the expression of TIP30 and VEGF (r=-0.428, P<0.05); no correlation was found between TIP30 and MVD(r=-0.065, P 0.05); the positive correlation was found between VEGF and MVD(r=0.684, P<0.01). Conclusion The positive expression rate of TIP30 in normal brain tissue is significantly higher than that in astrocytoma. The positive expression rate of TIP30 significantly decreases as the pathological grade of the astrocytoma increases; The expression of TIP30 and VEGF is negatively correlated in astrocytoma.     

神经肌电图检测在诊断早期格林巴利综合征的临床意义

目的:探究在格林巴利综合征的早期诊断中神经肌电图检测的临床意义。方法:选取2016年7月-2018年7月在我院进行治疗的早期格林巴利综合征患者45例,对其进行肌电图、神经传导速度以及F波的检测。结果:格林巴利患者安静状态下一般会出现数目较多的纤颤电位和正相电位,运动、感觉传导速度明显变慢且末端潜伏期增长,F波潜伏期增长且出现率降低。结论:在格林巴利综合征的早期诊断中应用神经肌电图进行检测在降低检测损害程度、提高患者治疗效果等方面具有明显优势,同时还能够尽量减少漏诊、误诊的情况出现,具有较为理想的诊断价值,可以在临床上进行广泛的推广和应用。     

培元抗癌汤结合曲妥珠单抗对乳腺癌患者临床疗效、肿瘤标志物影响研究

目的 探究培元抗癌汤结合曲妥珠单抗对乳腺癌患者临床疗效、肿瘤标志物影响。方法 研究纳入79例乳腺癌患者，均由该院2017年3月—2019年6月收治，采取随机数字表法将其分为观察组以及对照组，予以对照组患者(39例)曲妥珠单抗治疗，予以观察组患者(40例)培元抗癌汤结合曲妥珠单抗治疗，比较各组患者临床疗效、治疗前后中医症状积分(食后胀甚、腹胀纳呆、胸闷脘痞、大便溏薄、头晕目眩、舌淡苔白等)变化、治疗前后患者肿瘤标志物水平变化、不良反应情况、治疗前后生活质量核心量表(QLQ-C30)评分变化、治疗6个月、12个月后复发率。结果 观察组患者疾病控制率(57.50%,23/40)高于对照组(33.33%,13/39)(P<0.05);治疗前，两组患者食后胀甚、腹胀纳呆、胸闷脘痞、大便溏薄、头晕目眩、舌淡苔白等中医症状积分、糖蛋白125(CA125)及糖蛋白153(CA153)、癌胚抗原(CEA)水平、QLQ-C30量表评分比较(P>0.05),治疗后各组患者食后胀甚、腹胀纳呆、胸闷脘痞、大便溏薄、头晕目眩、舌淡苔白等中医症状积分、CA125、CA153及CEA等肿瘤标志物水平、QL...     

NRAGE影响人食管癌细胞放射抗性的作用及其机制研究

背景与目的：食管癌是全球威胁人类生命和健康的常见恶性肿瘤之一，中国每年食管癌发病人数占全球发病总人数的一半以上，以食管鳞癌最为常见。放疗是食管癌三大治疗手段之一，而放射抗性是导致其治疗失败的主要原因。神经营养因子受体相互作用MAGE类药物（neurotrophin receptor-interacting MAGE homolog，NRAGE）在放射抗性细胞株TE13R120中表达量明显高于亲本TE13细胞，且NRAGE亚细胞定位变化可能参与食管癌细胞放射抗性的形成。通过基因转染构建NRAGE稳定表达的食管癌细胞系，以进一步明确NRAGE基因与食管鳞癌细胞放射抗性的关系，分析该基因影响放射抗性的具体机制。方法：通过基因转染构建NRAGE稳定表达的食管癌细胞系。采用细胞克隆形成实验检测细胞放射敏感性，采用流式细胞术检测细胞周期及凋亡；细胞划痕、Transwell侵袭实验检测细胞迁移、侵袭能力，采用实时荧光定量聚合酶链反应（real-time fluorescence quantitive polymerase chain reaction，RTFQ-PCR）和蛋白质印迹法（Western blot）检测细胞中β-catenin表达情况。组间差异采用t检验或方差分析。结果：实验分为转染过表达组（Eca109/NRAGE组）和空白对照组（Eca109组）。Eca109/NRAGE细胞中NRAGE的表达量明显高于Eca109（t=29.65，P<0.05）。照射后Eca109/NRAGE细胞的放射抗性显著高于Eca109。流式细胞术检测结果显示，Eca109/NRAGE细胞中对射线抵抗性最强的S期细胞比例增加，对射线最敏感的G ₂ /M期减少。Eca109/NRAGE细胞凋亡率较Eca109细胞降低（t=3.268，P<0.05）。Eca109/NRAGE细胞迁移和侵袭能力均高于Eca109。RTFQ-PCR和Western blot检测结果显示，β-catenin的mRNA表达及蛋白水平在Eca109/NRAGE细胞中明显高于Eca109（t=15.87，P<0.05）。结论：NRAGE参与Eca109细胞放射抗性的形成，改变细胞的细胞周期分布和凋亡情况，影响细胞迁移及侵袭能力并可能影响食管癌细胞的放射敏感性，该作用可能与激活Wnt/β-catenin信号转导通路有关。