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排序方式: 共有251条查询结果,搜索用时 15 毫秒
1.
应用超频叠加治疗化学灼伤所致皮肤色素沉着15例疗效分析上海市化工职业病防治研究所陈德荣王教梅王柯我们自1995年以来,应用CEM-6A高级电脑美容美体机中的超频叠加项目和祛色精华素治疗化学灼伤所致皮肤色素沉着15例,取得良好效果。超声叠加治疗即指超声... 相似文献
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目的:探讨彩色多普勒超声检测Ⅱ型糖尿病患者下肢动脉病变的诊断应用。方法:选择100例Ⅱ型糖尿病患者,对其下肢共1000个节段进行彩色多普勒超声检查。结果:Ⅱ型糖尿病患者下肢动脉病变为血管内膜粗糙增厚,动脉腔内粥样斑块及血管部分或完全闭塞,彩色多普勒示血管内彩色充盈缺损。结论:彩色多普勒超声对II型糖尿病患者下肢动脉病变的诊断及追踪观察有良好的应用价值。 相似文献
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Objective To study the effects of prenatal and postnatal perfluorooetane sulfonate (PFOS) exposure on spatial learning and memory, N-methyl-D-aspartate receptor 2B (NR2B) mRNA and protein level in frontal cortex and hippoeampus of rat pups and to explore the mechanism of developmental neurotoxicity induced by PFOS. Methods Twenty-eight pregnant mrs were randomly divided into three groups in proportion of 3: 2: 2,including control group (C), low dose group (L) and high dose group (H) by means of randomized number table, which respectively received 0,7.2, 14.4 mg/kg PFOS feed from pregnancy day 0 to postnatal day(PND)30 by free feedings. The animal models of prenatal and postnatal non-exposure (CC), prenatal exposure (LC and HC), postnatal exposure (CL and CH), and prenatal and postnatal exposure (LL and HH) to PFOS were established by cross-fostering method. The spatial learning and memory were measured by water maze experiment,the NR2B mRNA levels in frontal cortex of rat pups was determined with semi-quantitative RT-PCR, NR2B protein express in cerebral cortex (frontal and tempera]cortex) and hippocampus (CAI, CA3, CA4 and DG regions) of rat pups was detected by immunohistochemistry. Results The escape latency of CL, CH, LL and HH groups pups in water maze experiment were (99. 83±25.77) s, (111.30±17. 82) s, (106. 40±18. 71) s, (107.70±16. 85) s, and longer as compared with CC group[(54.90±26.69)s](q value were 4.349,4.773,6.026 and 5.641, respectively,P<0. 01). The number of errors of HH group rat pups entering dead end was (22. 30±7.56) at the training day 4,and it was significantly higher than that of CC group (9. 80±4. 64) (q=5. 173,P<0. 01). The NR2B mRNA levels of frontal cortex of pups in HC group at PND1 ,and LC group, HC group and HH group at PND14 were (0. 167±0. 008), (0. 364±0. 035), (0. 341±0. 030) and (0. 328±0. 045) respectively,which were significantly lower than CC group (0.271±0.060) and (0.465±0.067) (q values were 3. 547, 3. 739, 4. 597 and 5. 006, respectively, P<0.05) . The results of immunohistochemistry indicated that NR2B protein express of the hippocampus CA1 region of pups in LC group was (0.091±0.005),and showed significant lower than CC group which was (0. 123±0.009) at PND1 (q=5. 209 ,P<0. 05). At PND14, the effect of PFOS extended to cerebral cortex and hippocampus regions. At PND28, the effects of PFOS were showed in hippocampus CA1, CA3 and temporal cortex regions. Conclusion Prenatal and postnatal exposure to PFOS should result in the spatial learning and memory damage,and the mechanism might be possibly involved in the decrease of NR2B level in cerebra lcortex and hippocampal formation regions. 相似文献
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采用TLC法定性鉴别复方丹参胶囊中的三七和丹参,并以HPLC法测定了其中水溶性成分丹酚酸B.鉴别三七时,样品先上固相萃取小柱用水和30%甲醇洗涤,以减少杂质对检测的干扰,再用甲醇洗脱,洗脱液用二氯甲烷-无水乙醇-水(70∶45∶6.5)为展开剂进行TLC,结果可使三七中的三七皂苷R1,人参皂苷Re、Rb1和Rg1达到有效分离.测定丹酚酸B含量时,采用C18柱,以甲醇-乙腈-甲酸-水(30∶10∶1∶59)为流动相,检测波长为286 nm.丹酚酸B在0.1~10 μg范围内线性关系良好.本研究所建立的定性和定量方法简便、准确、专属性强,可有效控制复方丹参胶囊的质量. 相似文献
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目的:建立白芍药材中19种有机氮类农药残留的毛细管电泳-质谱联用(CE-MS)检测方法。方法:采用CE-MS方法,熔融石英毛细管柱(70 cm×50μm),运行缓冲液为1%甲酸-15%甲醇水溶液(V/V,pH 2.2)。毛细管温度为25℃,分离电压为20 kV。质谱条件:采用电喷雾离子源(ESI),正离子模式检测;鞘液为90%甲醇-0.2%甲酸水溶液,流速为8μl.min-1;干燥气流速为6 L.min-1,干燥气温度为250℃;雾化气压力为5 psig;碎片电压为100 V,毛细管电压为5 000 V。结果:19种农药在一定浓度范围内呈线性关系,相关系数为0.9918~0.9986。19种农药的加样回收率为80.1%~108.4%,相对标准偏差(RSD)<20%(除螺环菌胺为22.3%,乙氧喹啉为29.2%外),检测限(LOD)为0.503μg.kg-1~10.1μg.kg-1。结论:该CE-MS分析方法专属性强、重现性好,适用于白芍药材中有机氮类农药的检测分析。 相似文献