Effect of electro-acupuncture combined with early rehabilitation on motor function and expressions of CD11b/CD18 and tumor necrosis factor-α in patients with acute cerebral infarction

Objective To observe the effect of electro-acupuncture combined with early rehabilitation on the motor function and expressions of the adhesion molecules CD11b and CD18 in the polymorphonuclear leucocytes (PMN) and monocytes and serum tumor necrosis factor-α (TNF-α) levels in patients with acute cerebral infarction (ACI). Methods A total of 165 ACI patients were randomly divided into control group (group A, n=50), conventional rehabilitation group (group B, n=50) and comprehensive rehabilitation group (group C, n=65). The expressions of CD11b and CD18 in the PMN and monocytes and serum TNF-α levels were determined before and at 1, 2, and 4 weeks after the treatment. Thirty-two healthy subjects were also recruited as the normal control group (group N). The neurological function of the subjects was evaluated by modified Edinburgh-Scandinavia stroke scale (MESSS) and Fugi-Meyer Assessment (FMA), and their activity of daily living (ADL) was assessed using Barthel index (BI). Results The CD11b/CD18 expression in the PMN and MN and serum TNF-α level in groups A, B and C were significantly higher than those in group N before and 1 week after the treatment (P<0.05). CD11b/CD18 expression and serum TNF-α level were significantly lower in groups B and C than in the group A at 1 week after the treatment, and significantly lower in group C than in group B (P<0.05). At 2 weeks of treatment, CD11b/CD18 and TNF-α were significantly lower in groups B and C than in the group A, being the lowest in group C (P<0.05). The scores of mESSS in both groups B and C were lower than that in group A, and the scores were lower in group C than in group B. Group C showed higher FMA scores than group B, both having higher scores than group A. At 4 weeks of treatment, the mESSS scores were significantly lower, hut the FMA and ADL score significantly higher in groups B and C than in the control group (P<0.05), and the differences were more obvious in group C. Groups B and C had greater effective rate than group A (P<0.05), and the rate was the highest in group C (P<0.05). Conclusion Electro-acupuncture combined with early rehabilitation promotes the recovery of motor function in ACI patients probably by regulating the expressions of the adhesion molecules CD11b and CD18 on the PMN and monocytes and the serum levels of TNF-α.     

Effect of electro-acupuncture combined with early rehabilitation on motor function and expressions of CD11b/CD18 and tumor necrosis factor-α in patients with acute cerebral infarction

Objective To observe the effect of electro-acupuncture combined with early rehabilitation on the motor function and expressions of the adhesion molecules CD11b and CD18 in the polymorphonuclear leucocytes (PMN) and monocytes and serum tumor necrosis factor-α (TNF-α) levels in patients with acute cerebral infarction (ACI). Methods A total of 165 ACI patients were randomly divided into control group (group A, n=50), conventional rehabilitation group (group B, n=50) and comprehensive rehabilitation group (group C, n=65). The expressions of CD11b and CD18 in the PMN and monocytes and serum TNF-α levels were determined before and at 1, 2, and 4 weeks after the treatment. Thirty-two healthy subjects were also recruited as the normal control group (group N). The neurological function of the subjects was evaluated by modified Edinburgh-Scandinavia stroke scale (MESSS) and Fugi-Meyer Assessment (FMA), and their activity of daily living (ADL) was assessed using Barthel index (BI). Results The CD11b/CD18 expression in the PMN and MN and serum TNF-α level in groups A, B and C were significantly higher than those in group N before and 1 week after the treatment (P<0.05). CD11b/CD18 expression and serum TNF-α level were significantly lower in groups B and C than in the group A at 1 week after the treatment, and significantly lower in group C than in group B (P<0.05). At 2 weeks of treatment, CD11b/CD18 and TNF-α were significantly lower in groups B and C than in the group A, being the lowest in group C (P<0.05). The scores of mESSS in both groups B and C were lower than that in group A, and the scores were lower in group C than in group B. Group C showed higher FMA scores than group B, both having higher scores than group A. At 4 weeks of treatment, the mESSS scores were significantly lower, hut the FMA and ADL score significantly higher in groups B and C than in the control group (P<0.05), and the differences were more obvious in group C. Groups B and C had greater effective rate than group A (P<0.05), and the rate was the highest in group C (P<0.05). Conclusion Electro-acupuncture combined with early rehabilitation promotes the recovery of motor function in ACI patients probably by regulating the expressions of the adhesion molecules CD11b and CD18 on the PMN and monocytes and the serum levels of TNF-α.     

Effect of electro-acupuncture combined with early rehabilitation on motor function and expressions of CD11b/CD18 and tumor necrosis factor-α in patients with acute cerebral infarction

Objective To observe the effect of electro-acupuncture combined with early rehabilitation on the motor function and expressions of the adhesion molecules CD11b and CD18 in the polymorphonuclear leucocytes (PMN) and monocytes and serum tumor necrosis factor-α (TNF-α) levels in patients with acute cerebral infarction (ACI). Methods A total of 165 ACI patients were randomly divided into control group (group A, n=50), conventional rehabilitation group (group B, n=50) and comprehensive rehabilitation group (group C, n=65). The expressions of CD11b and CD18 in the PMN and monocytes and serum TNF-α levels were determined before and at 1, 2, and 4 weeks after the treatment. Thirty-two healthy subjects were also recruited as the normal control group (group N). The neurological function of the subjects was evaluated by modified Edinburgh-Scandinavia stroke scale (MESSS) and Fugi-Meyer Assessment (FMA), and their activity of daily living (ADL) was assessed using Barthel index (BI). Results The CD11b/CD18 expression in the PMN and MN and serum TNF-α level in groups A, B and C were significantly higher than those in group N before and 1 week after the treatment (P<0.05). CD11b/CD18 expression and serum TNF-α level were significantly lower in groups B and C than in the group A at 1 week after the treatment, and significantly lower in group C than in group B (P<0.05). At 2 weeks of treatment, CD11b/CD18 and TNF-α were significantly lower in groups B and C than in the group A, being the lowest in group C (P<0.05). The scores of mESSS in both groups B and C were lower than that in group A, and the scores were lower in group C than in group B. Group C showed higher FMA scores than group B, both having higher scores than group A. At 4 weeks of treatment, the mESSS scores were significantly lower, hut the FMA and ADL score significantly higher in groups B and C than in the control group (P<0.05), and the differences were more obvious in group C. Groups B and C had greater effective rate than group A (P<0.05), and the rate was the highest in group C (P<0.05). Conclusion Electro-acupuncture combined with early rehabilitation promotes the recovery of motor function in ACI patients probably by regulating the expressions of the adhesion molecules CD11b and CD18 on the PMN and monocytes and the serum levels of TNF-α.     

Effect of electro-acupuncture combined with early rehabilitation on motor function and expressions of CD11b/CD18 and tumor necrosis factor-α in patients with acute cerebral infarction

Objective To observe the effect of electro-acupuncture combined with early rehabilitation on the motor function and expressions of the adhesion molecules CD11b and CD18 in the polymorphonuclear leucocytes (PMN) and monocytes and serum tumor necrosis factor-α (TNF-α) levels in patients with acute cerebral infarction (ACI). Methods A total of 165 ACI patients were randomly divided into control group (group A, n=50), conventional rehabilitation group (group B, n=50) and comprehensive rehabilitation group (group C, n=65). The expressions of CD11b and CD18 in the PMN and monocytes and serum TNF-α levels were determined before and at 1, 2, and 4 weeks after the treatment. Thirty-two healthy subjects were also recruited as the normal control group (group N). The neurological function of the subjects was evaluated by modified Edinburgh-Scandinavia stroke scale (MESSS) and Fugi-Meyer Assessment (FMA), and their activity of daily living (ADL) was assessed using Barthel index (BI). Results The CD11b/CD18 expression in the PMN and MN and serum TNF-α level in groups A, B and C were significantly higher than those in group N before and 1 week after the treatment (P<0.05). CD11b/CD18 expression and serum TNF-α level were significantly lower in groups B and C than in the group A at 1 week after the treatment, and significantly lower in group C than in group B (P<0.05). At 2 weeks of treatment, CD11b/CD18 and TNF-α were significantly lower in groups B and C than in the group A, being the lowest in group C (P<0.05). The scores of mESSS in both groups B and C were lower than that in group A, and the scores were lower in group C than in group B. Group C showed higher FMA scores than group B, both having higher scores than group A. At 4 weeks of treatment, the mESSS scores were significantly lower, hut the FMA and ADL score significantly higher in groups B and C than in the control group (P<0.05), and the differences were more obvious in group C. Groups B and C had greater effective rate than group A (P<0.05), and the rate was the highest in group C (P<0.05). Conclusion Electro-acupuncture combined with early rehabilitation promotes the recovery of motor function in ACI patients probably by regulating the expressions of the adhesion molecules CD11b and CD18 on the PMN and monocytes and the serum levels of TNF-α.     

Effect of electro-acupuncture combined with early rehabilitation on motor function and expressions of CD11b/CD18 and tumor necrosis factor-α in patients with acute cerebral infarction

Objective To observe the effect of electro-acupuncture combined with early rehabilitation on the motor function and expressions of the adhesion molecules CD11b and CD18 in the polymorphonuclear leucocytes (PMN) and monocytes and serum tumor necrosis factor-α (TNF-α) levels in patients with acute cerebral infarction (ACI). Methods A total of 165 ACI patients were randomly divided into control group (group A, n=50), conventional rehabilitation group (group B, n=50) and comprehensive rehabilitation group (group C, n=65). The expressions of CD11b and CD18 in the PMN and monocytes and serum TNF-α levels were determined before and at 1, 2, and 4 weeks after the treatment. Thirty-two healthy subjects were also recruited as the normal control group (group N). The neurological function of the subjects was evaluated by modified Edinburgh-Scandinavia stroke scale (MESSS) and Fugi-Meyer Assessment (FMA), and their activity of daily living (ADL) was assessed using Barthel index (BI). Results The CD11b/CD18 expression in the PMN and MN and serum TNF-α level in groups A, B and C were significantly higher than those in group N before and 1 week after the treatment (P<0.05). CD11b/CD18 expression and serum TNF-α level were significantly lower in groups B and C than in the group A at 1 week after the treatment, and significantly lower in group C than in group B (P<0.05). At 2 weeks of treatment, CD11b/CD18 and TNF-α were significantly lower in groups B and C than in the group A, being the lowest in group C (P<0.05). The scores of mESSS in both groups B and C were lower than that in group A, and the scores were lower in group C than in group B. Group C showed higher FMA scores than group B, both having higher scores than group A. At 4 weeks of treatment, the mESSS scores were significantly lower, hut the FMA and ADL score significantly higher in groups B and C than in the control group (P<0.05), and the differences were more obvious in group C. Groups B and C had greater effective rate than group A (P<0.05), and the rate was the highest in group C (P<0.05). Conclusion Electro-acupuncture combined with early rehabilitation promotes the recovery of motor function in ACI patients probably by regulating the expressions of the adhesion molecules CD11b and CD18 on the PMN and monocytes and the serum levels of TNF-α.     

Effect of electro-acupuncture combined with early rehabilitation on motor function and expressions of CD11b/CD18 and tumor necrosis factor-α in patients with acute cerebral infarction

Objective To observe the effect of electro-acupuncture combined with early rehabilitation on the motor function and expressions of the adhesion molecules CD11b and CD18 in the polymorphonuclear leucocytes (PMN) and monocytes and serum tumor necrosis factor-α (TNF-α) levels in patients with acute cerebral infarction (ACI). Methods A total of 165 ACI patients were randomly divided into control group (group A, n=50), conventional rehabilitation group (group B, n=50) and comprehensive rehabilitation group (group C, n=65). The expressions of CD11b and CD18 in the PMN and monocytes and serum TNF-α levels were determined before and at 1, 2, and 4 weeks after the treatment. Thirty-two healthy subjects were also recruited as the normal control group (group N). The neurological function of the subjects was evaluated by modified Edinburgh-Scandinavia stroke scale (MESSS) and Fugi-Meyer Assessment (FMA), and their activity of daily living (ADL) was assessed using Barthel index (BI). Results The CD11b/CD18 expression in the PMN and MN and serum TNF-α level in groups A, B and C were significantly higher than those in group N before and 1 week after the treatment (P<0.05). CD11b/CD18 expression and serum TNF-α level were significantly lower in groups B and C than in the group A at 1 week after the treatment, and significantly lower in group C than in group B (P<0.05). At 2 weeks of treatment, CD11b/CD18 and TNF-α were significantly lower in groups B and C than in the group A, being the lowest in group C (P<0.05). The scores of mESSS in both groups B and C were lower than that in group A, and the scores were lower in group C than in group B. Group C showed higher FMA scores than group B, both having higher scores than group A. At 4 weeks of treatment, the mESSS scores were significantly lower, hut the FMA and ADL score significantly higher in groups B and C than in the control group (P<0.05), and the differences were more obvious in group C. Groups B and C had greater effective rate than group A (P<0.05), and the rate was the highest in group C (P<0.05). Conclusion Electro-acupuncture combined with early rehabilitation promotes the recovery of motor function in ACI patients probably by regulating the expressions of the adhesion molecules CD11b and CD18 on the PMN and monocytes and the serum levels of TNF-α.     

Effect of electro-acupuncture combined with early rehabilitation on motor function and expressions of CD11b/CD18 and tumor necrosis factor-α in patients with acute cerebral infarction

Objective To observe the effect of electro-acupuncture combined with early rehabilitation on the motor function and expressions of the adhesion molecules CD11b and CD18 in the polymorphonuclear leucocytes (PMN) and monocytes and serum tumor necrosis factor-α (TNF-α) levels in patients with acute cerebral infarction (ACI). Methods A total of 165 ACI patients were randomly divided into control group (group A, n=50), conventional rehabilitation group (group B, n=50) and comprehensive rehabilitation group (group C, n=65). The expressions of CD11b and CD18 in the PMN and monocytes and serum TNF-α levels were determined before and at 1, 2, and 4 weeks after the treatment. Thirty-two healthy subjects were also recruited as the normal control group (group N). The neurological function of the subjects was evaluated by modified Edinburgh-Scandinavia stroke scale (MESSS) and Fugi-Meyer Assessment (FMA), and their activity of daily living (ADL) was assessed using Barthel index (BI). Results The CD11b/CD18 expression in the PMN and MN and serum TNF-α level in groups A, B and C were significantly higher than those in group N before and 1 week after the treatment (P<0.05). CD11b/CD18 expression and serum TNF-α level were significantly lower in groups B and C than in the group A at 1 week after the treatment, and significantly lower in group C than in group B (P<0.05). At 2 weeks of treatment, CD11b/CD18 and TNF-α were significantly lower in groups B and C than in the group A, being the lowest in group C (P<0.05). The scores of mESSS in both groups B and C were lower than that in group A, and the scores were lower in group C than in group B. Group C showed higher FMA scores than group B, both having higher scores than group A. At 4 weeks of treatment, the mESSS scores were significantly lower, hut the FMA and ADL score significantly higher in groups B and C than in the control group (P<0.05), and the differences were more obvious in group C. Groups B and C had greater effective rate than group A (P<0.05), and the rate was the highest in group C (P<0.05). Conclusion Electro-acupuncture combined with early rehabilitation promotes the recovery of motor function in ACI patients probably by regulating the expressions of the adhesion molecules CD11b and CD18 on the PMN and monocytes and the serum levels of TNF-α.     

Effect of electro-acupuncture combined with early rehabilitation on motor function and expressions of CD11b/CD18 and tumor necrosis factor-α in patients with acute cerebral infarction

Objective To observe the effect of electro-acupuncture combined with early rehabilitation on the motor function and expressions of the adhesion molecules CD11b and CD18 in the polymorphonuclear leucocytes (PMN) and monocytes and serum tumor necrosis factor-α (TNF-α) levels in patients with acute cerebral infarction (ACI). Methods A total of 165 ACI patients were randomly divided into control group (group A, n=50), conventional rehabilitation group (group B, n=50) and comprehensive rehabilitation group (group C, n=65). The expressions of CD11b and CD18 in the PMN and monocytes and serum TNF-α levels were determined before and at 1, 2, and 4 weeks after the treatment. Thirty-two healthy subjects were also recruited as the normal control group (group N). The neurological function of the subjects was evaluated by modified Edinburgh-Scandinavia stroke scale (MESSS) and Fugi-Meyer Assessment (FMA), and their activity of daily living (ADL) was assessed using Barthel index (BI). Results The CD11b/CD18 expression in the PMN and MN and serum TNF-α level in groups A, B and C were significantly higher than those in group N before and 1 week after the treatment (P<0.05). CD11b/CD18 expression and serum TNF-α level were significantly lower in groups B and C than in the group A at 1 week after the treatment, and significantly lower in group C than in group B (P<0.05). At 2 weeks of treatment, CD11b/CD18 and TNF-α were significantly lower in groups B and C than in the group A, being the lowest in group C (P<0.05). The scores of mESSS in both groups B and C were lower than that in group A, and the scores were lower in group C than in group B. Group C showed higher FMA scores than group B, both having higher scores than group A. At 4 weeks of treatment, the mESSS scores were significantly lower, hut the FMA and ADL score significantly higher in groups B and C than in the control group (P<0.05), and the differences were more obvious in group C. Groups B and C had greater effective rate than group A (P<0.05), and the rate was the highest in group C (P<0.05). Conclusion Electro-acupuncture combined with early rehabilitation promotes the recovery of motor function in ACI patients probably by regulating the expressions of the adhesion molecules CD11b and CD18 on the PMN and monocytes and the serum levels of TNF-α.     

细胞周期素依赖性蛋白激酶抑制剂治疗实验性单纯疱疹性脑炎的效果及其机制

目的探讨细胞周期素依赖性蛋白激酶(CDK)抑制剂(Olomoucine)治疗实验性单纯疱疹性脑炎(HSE)效果及其机制。方法45只小鼠随机分为1型单纯疱疹病毒(HSV-1)假感染组、感染对照组和Olo-moucine治疗组,每组15只。应用流式细胞术测定各组脑细胞表面抗原CD11b的表达;使用逆转录-聚合酶链反应方法检测肿瘤坏死因子-α(TNF-α)、白介素-6(IL-6)的表达;TUNEL染色检测神经细胞凋亡,脑组织病理切片观察组织形态学变化。另40只小鼠HSV-1感染后随机分为Acyclovir治疗组和Olomoucine治疗组,每组20只;2周后应用Kaplan-Meier法进行生存率比较。结果HSV-1感染后脑组织中CD11b阳性细胞、TNF-α、IL-6表达较假感染组显著增高(均P<0.01);TUNEL阳性细胞数明显增多;脑组织细胞变性、出血及炎性细胞浸润。Olomoucine治疗后,脑组织中CD11b阳性细胞、TNF-α、IL-6表达明显降低(均P<0.01),神经细胞凋亡减少,脑组织病理改变减轻。Olomoucine治疗组生存率较Acydovir治疗组显著提高(P<0.05)。结论Olo-mo...     

早期康复治疗对急性脑梗死患者临床及血清炎性因子水平的影响

目的 观察早期康复治疗对急性脑梗死(ACI) 患者的运动功能及日常生活能力、血清超敏C- 反应蛋白(hs-CRP) 、白细胞介素-1( IL- 1)、白细胞介素- 6(IL- 6)和肿瘤坏死因子-α(TNF-α)水平的影响.方法 将80例ACI患者随机分为两组,康复组40例采用早期康复治疗和常规中西药物治疗,对照组40例仅采用常规中西药物治疗;测定两组患者治疗前和治疗后2和4周后外周血hs-CRP、IL- 1 IL- 6和TNF-α水平,运动功能采用Fugl-Meyer运动功能积分法(FMA)评定,日常生活能力采用改良Barthel指数评分(MBI).结果 康复组治疗后2和4周时外周血hs-CRP、IL- 1 和IL- 6水平低于对照组(P<0.05);康复组FMA、MBI上升幅度明显高于对照组(P＜0.05).结论 早期康复干预可降低急性脑梗死患者患者血清hs-CRP、IL- 1 、IL- 6 和TNF-α水平,并能明显改善肢体运动功能,提高日常生活能力.     

重症肌无力患者外周血单个核细胞TNF-α和IL-6水平在疾病转归中的动态变化

目的 研究重症肌无力(MG)患者在激素或免疫抑制剂干预治疗前后肿瘤坏死因子-α(TNF-α)和白细胞介素-6(IL-6)水平的动态变化,探讨TNF-α和IL-6在MG发病机制中的作用.方法 以符合诊断标准的30例各型MG患者(MG组)和20名正常人(对照组)为观察对象,在治疗前后进行肌无力评分并检测外周血单个核细胞TNF-α和IL-6水平变化.结果 MG组TNF-α和IL-6水平治疗前均明显高于对照组(P<0.01);治疗2周后较治疗前明显降低(P<0.01);全身型MG患者高于眼肌型患者,Ⅱb型高于Ⅱa型(P<0.05).MG患者治疗前后肌无力评分与TNF-α和IL-6水平均呈正相关(r=0.954、0.871、0.844、0.797,P<0.05),治疗前后评分差值与TNF-α和IL-6水平差值呈正相关(r=0.857、0.784,P<0.01).结论 MG患者细胞免疫功能异常,肌无力轻重程度与TNF-α和IL-6分泌水平呈正相关,TNF-α和IL-6分泌水平变化可反映干预治疗疗效.     

急性缺血性脑卒中患者外周血白细胞CD11c/CD18表达的变化

目的探讨外周血白细胞CD11c/CD18表达与急性缺血性脑卒中(acute ischemic stroke,AIS)发病的关系。方法采用流式细胞术和单克隆抗体标记测定28例AIS患者(AIS组)发病72h内及病程第7d2次外周血中性粒细胞(PMN)和单核细胞(MNL)表面CD11c、CD18的表达量,以平均荧光强度(MFI)表示其相对含量,并与28名健康对照者(对照组)比较。结果AIS组发病72h内外周血PMN表面CD11c、CD18MFI分别为20.82±5.88、218.25±89.00;病程7d时为18.60±5.52、185.52±68.44;均显著高于对照组CD11c、CD18MFI(15.63±3.01、150.76±41.20);发病72h内外周血MNL表面CD11c、CD18MFI分别为34.78±14.56、286.75±95.50,病程7d时为31.97±14.47、247.00±88.06,均显著高于对照组CD11c、CD18MFI(20.20±8.50和186.38±52.97)(P<0.05~0.01);发病7d时CD11c、CD18MFI虽有所下降,但与发病72h内比较差异无显著性(P>0.05)。结论AIS时激活的PMN、MNL表面β2整合素CD11c、CD18表达上调,CD11c、CD18可能参与了炎症反应。     

雌二醇对实验性自身免疫性脑脊髓炎调节性T细胞及细胞因子的影响

目的 探讨雌二醇(E2)对实验性自身免疫性脑脊髓炎(EAE)大鼠调节性T细胞及细胞因子的影响.方法 将大鼠随机分为E2干预组和EAE对照组,记录其临床评分, 测定外周血CD4+CD25+、CD4+CD25+Foxp3+ T细胞水平以及TNF-α、IL-12表达水平,并观察腰膨大处炎细胞浸润情况.结果 (1)E2干预组发病率仅为30% ,低于EAE对照组(100%), 且发病高峰延迟;临床评分E2干预组[(1.8±1.3)分]比EAE对照组[(3.4±0.5)分]低, 差异有统计学意义(P<0.05);(2)E2干预组CD4+CD25+/CD4+ T细胞比值 (5.6±0.9)、CD4+CD25+Foxp3+/CD4+CD25+ T细胞比值(9.3±1.0)均高于EAE对照组[分别为(4.4±0.9)与(7.6±0.8)], 差异有统计学意义(均P<0.05);(3)血管"套袖"样改变EAE对照组较E2干预组明显;(4)E2干预组TNF-α及IL-12表达明显低于EAE对照组, 差异有统计学意义(均P<0.05).结论 E2可能通过改变调节性T细胞比例及细胞因子表达参与EAE免疫调节过程, 推测EAE临床症状缓解可能也与此机制相关.     

醒脑静联用生脉注射液对脑出血患者CD62P和炎症因子表达的影响及其临床作用

目的探讨醒脑静联用生脉注射液对脑出血患者循环中CD62P和肿瘤坏死因子-α(TNF-α)、白细胞介素-6(IL-6)表达的影响及其临床作用。方法纳入2014年4月至2015年10月我院神经内科经症状、体征和辅助检查确诊为脑出血的患者152例,随机分为治疗组78例,对照组74例。对照组采用常规治疗方法,治疗组在常规治疗方法的基础上加用醒脑静联用生脉注射液,观察两组患者治疗前后循环中CD62P、TNF-α、IL-6水平的变化,以及美国国立卫生研究院脑卒中量表(NIHSS)、日常生活能力评估量表(ADL)评分的变化。结果经过治疗7 d和14 d后,治疗组循环中CD62P、TNF-α、IL-6的水平均显著低于对照组(P0.05)。经过治疗7 d和14 d后,治疗组NIHSS评分显著低于对照组(P0.05);治疗组ADL评分显著高于对照组(P0.05)。结论醒脑静联用生脉注射液可能通过降低体内血小板激活水平,减少炎症因子表达,从而达到保护脑出血患者神经系统功能的效果。     

糖皮质激素对多发性硬化患者外周血淋巴细胞CD80和CD4+CD25+T细胞表达的影响

目的探讨糖皮质激素(GC)对多发性硬化(MS)患者外周血淋巴细胞CD80和CD4+CD25+T细胞表达的影响。方法利用流式细胞仪检测21例MS急性期患者GC治疗前后外周血淋巴细胞CD80和CD4+CD25+T细胞阳性率,并与正常对照组比较;比较MS患者治疗前后扩展功能障碍状况量表(EDSS)评分的变化。结果MS患者急性期外周血淋巴细胞CD80的阳性率[(5.031±1.782)%]较正常对照组[(6.436±2.035)%]明显下降(P<0.05),经GC治疗后CD80的阳性率[(6.467±1.882)%]明显增高(P<0.01);CD4+CD25+T细胞阳性率治疗前后与正常对照组间差异均无统计学意义;治疗后EDSS评分[(3.64±1.79)分]较治疗前[(4.26±1.68)分]明显下降(P<0.01)。结论GC可上调MS患者淋巴细胞CD80的表达,抑制细胞免疫,促进MS病情缓解。     

急性缺血性卒中患者外周血白细胞表面黏附分子β2整合素CD11c、CD18表达的研究

目的探讨急性缺血性卒中(AIS)患者外周血中性粒细胞(PMN)和单核细胞(MNL)表面黏附分子β2整合素CD11c、CD18表达的变化及意义.方法采用流式细胞术,单克隆抗体标记测定28例AIS患者(AIS组)发病72 h内及7 d时外周血PMN、MNL表面CD11c、CD18的表达量,并与 28名健康对照者(健康对照组)比较.结果 AIS组患者发病72 h内PMN、MNL表面CD11c、CD18表达的平均荧光强度分别为20.82±5.88、218.25±89.00、34.78±14.56、和286.75±95.50,7 d时分别为18.60±5.52、185.52±68.44、31.97±14.47和247.00±88.06;显著高于健康对照组(15.63 ±3.01、150.76±41.20、 20.20±8.50和186.38±52.97)(均P<0.01);发病7 d时CD11c、CD18的表达虽有所下降, 但与72 h检测结果相比差异无显著性.结论 AIS时激活的 PMN、MNL表面β2整合素CD11c、CD18表达上调, CD11c、CD18可能参与了AIS的炎性反应.     

奥扎格雷对急性脑梗死患者血小板CD62p和CD63表达的影响及其疗效

目的观察奥扎格雷对急性脑梗死(ACI)患者血小板CD62p、CD63表达的影响及其疗效。方法将64例ACI患者随机分为奥扎格雷治疗组和血塞通治疗组(对照组),采用流式细胞术检测ACI患者治疗前后及正常人(正常组)血小板CD62p、CD63的表达;观察奥扎格雷治疗组和对照组的临床疗效并进行比较。结果ACI患者血小板CD62p、CD63表达水平明显高于正常组(均P<0.01);奥扎格雷治疗组与对照组治疗后血小板CD62p、CD63表达水平较治疗前均有明显下降(P<0.05～0.01),奥扎格雷治疗组又明显低于对照组,差异有显著性(均P<0.05)。奥扎格雷治疗组的基本痊愈率、显著进步率、总有效率明显高于对照组(均P<0.05)。结论ACI发病后血小板CD62p、CD63表达水平显著增高;奥扎格雷有明显抑制血小板表达CD62p、CD63的作用,对ACI的治疗效果显著。     

左旋多巴治疗对实验性帕金森病大鼠黑质纹状体TNF-α表达的影响

目的 研究左旋多巴(L-dopa)治疗对实验性帕金森病(PD)大鼠黑质纹状体肿瘤坏死因子(TNF-α)表达的影响.方法 黑质定位注射6-羟多巴胺(6-OHDA)制备偏侧PD大鼠模型,经50～100 mg/kg体重L-dopa灌胃治疗4周后,检测双侧额叶皮质、黑质和纹状体区域TNF-α的表达.结果 6-OHDA损毁侧黑质和纹状体TNF-α含量和阳性细胞面密度分别显著高于健侧(均P<0.05).损毁侧与健侧TNF-α含量的比率随L-dopa治疗用量的增加而增高,且均显著高于对照组(P<0.05).结论 L-dopa治疗进一步加剧了PD大鼠黑质纹状体区域TNF-α的高表达.     

急性脑梗死患者中性粒细胞粘附分子表达的变化及意义探讨

目的探讨急性脑梗死(cerebral infarction,CI)患者中性粒细胞(polymorphonuclear neutrophil,PMN)表面粘附分子CD62L和CD11b/CD18表达及其意义。方法选择急性血栓形成性脑梗死患者40例,年龄、性别等与之匹配的健康志愿者30例作为对照。运用流式细胞仪对所有对象检测外周血中PMN表面粘附分子CD62L和CD11b/CD18表达。结果相对于正常组粘附分子CD62L平均抗体阳性表达率(73.316±1.276)%,CI组(61.058±8.925)%显著降低(P<0.001);相对于正常组CD11b/CD18平均阳性表达率(20.031±0.540)%,CI组(55,598±0.540)%显著升高。CI组内部CD62L、CD11b/CD18相关分析,二者呈明显负相关,相关系数为r=-0.259(P<0.001)。结论在急性血栓形成性脑梗死的急性期,PMN处于活化状态,表现为粘附分子CD62L表达的下调和CD11b/CD18表达的上调。以细胞粘附为表现的PMN活化加快了血栓的进程,可能是血栓形成的重要发病原因之一。     

加巴喷丁治疗糖尿病周围神经痛患者的临床疗效及对血清TNF-α、IL-6的影响

目的通过观察加巴喷丁(GBP)对糖尿病周围神经痛(DPN)患者的疗效以及血清肿瘤坏死因子(TNF-α)、白介素-6(IL-6)的影响。方法 47例DPN患者按是否服用GBP随机分为GBP组24例与DPN对照组23例,糖尿病无神经痛患者25例为糖尿病(DM)组,成年健康体检者25例为正常对照(NC)组。观察DPN患者治疗前、治疗1、2、4 w后的视觉模拟评分(VAS)分值变化以及药物副作用。ELISA法测定血清中TNF-α、IL-6的水平。结果治疗前GBP组与DPN对照组VAS比较,差异无统计学意义(P>0.05);GBP组治疗后1、2、4 w VAS分值均显著下降,与治疗前及同期DPN对照组比较,差异有统计学意义(P<0.01);DPN对照组各时期VAS分值差异无统计学意义(P>0.05)。DM、DPN各组血清TNF-α、IL-6均高于NC组,差异有统计学意义(P<0.01),DPN组血清TNF-α、IL-6又高于DM组,差异有统计学意义(P<0.01,P<0.05);与治疗前比较,GBP组治疗后血清TNF-α、IL-6水平下降,差异有统计学意义(P<0.01,P<0.05),DPN对照组治疗前后血清TNF-α、IL-6水平无明显变化,差异无统计学意义(P>0.05)。治疗过程中有1例出现嗜睡,3例出现眩晕,但均可耐受,观察期间血常规、肝肾功能无明显变化。结论加巴喷丁治疗糖尿病神经痛效果确切,副作用轻微,可降低机体血清TNF-α和IL-6水平。