共查询到20条相似文献,搜索用时 62 毫秒
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目的:在同一系统中分离并测定复方丹黄口服液中的黄芩甙和丹参素的含量,为丹黄口服液提供一个简便、快速、准确的质控指标。方法:采用反相高效液相色谱法,以离子抑制技术分离复方中成药制剂丹黄口服液中的黄芩甙和丹参素,并用外标法测定它们在丹黄口服液中的含量。结果:黄芩甙和丹参素各浓度重现性测定,其变异分别为069%~45%、024%~353%;黄芩甙高中低浓度回收率为9968%、10193%、8875%,丹参素高中低浓度回收率为9976%、10027%、9902%。两批丹黄口服液制剂样品中的黄芩甙和丹参素含量分别为1243μg/ml(1254μg/ml)和1598μg/ml(1602μg/ml)。结论:该方法重现性好,回收率高,可作为丹黄口服液的质控方法。两批制剂中黄芩甙和丹参素含量较高,达到治疗浓度,可应用于临床。 相似文献
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离子对高效液相色谱法测定注射用美洛西林钠—舒巴坦钠含量 总被引:7,自引:0,他引:7
目的:建立反相离子对高效液相色谱法测定注射用美洛西林钠-舒巴坦钠含量。方法:采用μBondapakC18色谱柱,0005mol·L-1氢氧化四丁基铵-乙腈(68∶32,pH50±01)为流动相,230nm为检测波长,30℃柱温下对美洛西林和舒巴坦进行分离和测定。结果:能同时测定制剂中美洛西林和舒巴坦的含量。美洛西林在025~15mg·mL-1,舒巴坦在0125~075mg·mL-1范围内,峰面积与其浓度呈良好线性关系(r=09999);平均回收率(n=5)美洛西林为9976%(RSD=092%),舒巴坦为9966%(RSD=085%)。结论:该法简便、快速、准确,适用于该药品生产及临床应用中的质量监控。 相似文献
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反相高效液相色谱法测定阿司匹林可待因片中磷酸可待因和阿司匹林的含量 总被引:4,自引:0,他引:4
目的:建立一种用高效液相色谱法检测复方制剂中磷酸可待因和阿司匹林含量的方法。方法:用C18ODS为固定相。甲醇-003mol·L-1醋酸钠(用冰醋酸调pH至35)(1∶25)为流动相。UV检测波长280nm。结果:该方法回收率为磷酸可待因1004%,RSD=13%(n=6);阿司匹林994%,RSD=098%(n=6)。结论:该法不需经提取分离,溶解后直接进样。简便、快速,准确可靠,适合于生产中使用。 相似文献
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高效液相色谱法测定胃利胶囊中橙皮苷的含量 总被引:10,自引:0,他引:10
目的:建立胃利胶囊中橙皮苷含量测定方法。方法:用高效液相色谱法测定。选用YWG-C18分析柱(46mm×250mm,10μm),流动相:乙腈-水-甲醇-磷酸(20∶796∶04∶001),检测波长:283nm,流速:15mL·min-1,柱温:室温。结果:本法简便、灵敏、准确。线性范围:02~15μg(r=09993)。平均回收率:9939%,RSD=35%。结论:建立的定量方法可用于胃利胶囊的质量控制标准。 相似文献
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他唑巴坦及其制剂注射用哌拉西林钠/他唑巴坦钠的HPLC含量测定方法的建立 总被引:5,自引:0,他引:5
目的:建立他唑巴坦及其制剂注射用哌拉西林钠/他唑巴坦钠的HPLC含量测定方法。方法:他唑巴坦的HPLC含量测定色谱条件:C18柱,流动相:pH40的乙腈-005mol·L-1的磷酸二氢钾水溶液-25mmol·L-1四丁基氢氧化铵(180∶805∶15),检测波长为230nm,流速:1mL·min-1;注射用哌拉西林钠/他唑巴坦钠的HPLC含量测定色谱条件:C18柱,流动相为pH35的甲醇-水-25mmol·L-1四丁基氢氧化铵(450∶535∶15),检测波长为230nm,流速:1mL·min-1。结果:他唑巴坦的HPLC含量测定方法的线性范围为01~32mg·mL-1,日内RSD为03%,日间RSD为042%~065%;注射用哌拉西林钠/他唑巴坦钠的HPLC含量测定方法中,他唑巴坦的线性范围为005~10mg·mL-1,日内RSD为11%,日间RSD为11%~15%,哌拉西林的线性范围为04~16mg·mL-1,日内RSD为035%,日间RSD为027%~046%。结论:本法简便,快速,准确可靠。 相似文献
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高效液相色谱法测定洋金花药材中东莨菪碱的含量 总被引:17,自引:0,他引:17
目的:建立测定洋金花药材中东莨菪碱含量的高效液相色谱法。方法:采用Hypersil(5μm,46mm×200mm)色谱柱,以甲醇-水(53∶47,水中含20mmol·L-1醋酸钠,002%三乙胺,03%四氢呋喃,pH为686)为流动相,紫外检测波长215nm,外标法定量。结果:东莨菪碱浓度在2~666μg·mL-1范围内呈线性关系,方法回收率为9909%(n=6),RSD为074%。结论:此法灵敏度高,重现性好。 相似文献
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小儿速效感冒冲剂中对-乙酰氨基酚的测定 总被引:1,自引:0,他引:1
《实用药物与临床》1998,(4)
目的:通过试验以求快速测定小儿速效感冒冲剂主药的含量。方法:采用差式分光光度法(△A)[1,2]测定小儿速效感冒冲剂中对-乙酰氨基酚的含量,并与亚硝酸钠法进行对照。结果:△A平均回收率为993%,CV为050%(n=5),两法比较P>005,无显著性差异。结论:△A法测定对-乙酰氨基酚含量可以排除其它组份干扰,快速准确。 相似文献
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Basic fibroblast growth factor protected forebrain against ischemia-reperfusion damage in rats 总被引:1,自引:0,他引:1
目的:研究碱性成纤维细胞生长因子(bFGF)对大鼠脑缺血再灌注损伤的影响.方法:电灼闭塞椎动脉并夹闭颈动脉,使大鼠前脑缺血20分钟后,放开双侧颈动脉再灌.用高压液相色谱法测定纹状体中多巴胺(DA)类物质含量并用原子吸收分光光度法测定前脑中钠、钾含量.结果:再灌6小时后,纹状体中DA含量由对照的(99±16)μg·g-1(蛋白)减少至(70±20)μg·g-1蛋白;脑含水量由7734%±019%增加到796%±06%;钠含量由(93±06)mg·g-1(脑干重)增加到(105±06)mg·g-1(脑干重).再灌开始后即刻静注bFGF45μg·kg-1·h-1共3h可阻止DA的减少和水、钠含量的升高.组织学分析也表明bFGF可减轻神经元所受损伤.结论:bFGF可以保护神经细胞对抗脑缺血再灌损伤. 相似文献
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The authors administered sulpiride in 14 acute stuporose patients (2 depressives and 12 schizophrenics). The initial dosage was 100–200 mg by a single intramuscular injection and as soon as oral administration became possible, it was replaced by a single daily dose (per os) of about 10 mg or over per kg of body weight given in the morning. In schizophrenics stupor disappeared within 2 to 10 days and in depressives in 10 and 14 days respectively. 相似文献
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Kazuhiko Abe 《Psychopharmacology》1975,43(1):101-101
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HPLC法测定舒必利片的含量 总被引:1,自引:0,他引:1
目的测定舒必利片中舒必利的含量。方法采用HPLC法测定舒必利片中舒必利的含量。结果舒必利在50~500mg/ml的浓度范围内呈良好的线性,γ=0.9999,平均回收率为99.0%(n=5),RSD为0.82%(n=5)。结论所建立的方法能可靠准确地进行含量测定。 相似文献
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目的:制备舒必利分散片并建立其质量控制方法。方法:正交试验考察处方中组分的影响因素,以崩解时限为指标对舒必利分散片处方进行优选;采用紫外分光光度法测定分散片中舒必利的含量和溶出度,测定波长为291nm。结果:最佳处方为崩解剂交联聚乙烯吡咯烷酮(PVPP)-XL用量10%(内加),PVPP-XL10用量2%(外加),填充剂微晶纤维素用量20%,黏合剂PVP浓度5%。优选处方的分散片崩解时间小于1min,分散均匀,通过2目筛;舒必利检测浓度线性范围为20~120mg.L-1(r=0.9999),平均回收率99.96%(RSD=0.66%,n=6);样品在15min时累积溶出百分率大于95%。结论:该分散片符合《中国药典》2005年版的相关规定。 相似文献
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目的:建立人血清中舒必利的测定方法,为临床应用和药化动力学研究提供技术参考.方法:采用高效液相色谱法检测,流动相为甲醇-乙腈-5 mmol/L磷酸二氢钠(30:3:67,V/V),腈基分离柱,UV波长254 nm,以对二甲氨基苯甲醛为内标,氯仿提取.结果:线性范围为50~1 500μg/L,平均回收率为99.62%,日内、日间精密度RSD分别为2.54%和3.67%,且常用的其他抗精神病药及安定类药物不干扰测定.结论:方法简单,分析速度快,灵敏度高,适用临床血药浓度测定及药代动力学研究. 相似文献
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Purpose. (a) To improve the absorption of sulpiride (SP) through the intestinal wall by incorporating it together with sodium decanoate (SD) into erodible matrices, designed to synchronize the release of SP and SD over different periods of time; (b) to test, in vivo the hypothesis that this simultaneous release increases SP absorption from the intestinal lumen.
Methods. Matrix tablets, possessing different erosion rates, were prepared by changing the ratios between SD and hydroxypropyl methylcellulose (HPMC). The amounts of HPMC varied from 2.5% to 17% w/w. Double layer tablets, containing similar amounts of SP, SD, and HPMC were used as nonsynchronous controls. The erosion kinetics of the tablets was assessed gravimetrically in vitro in USP basket dissolution apparatus and in vivo in the intestine of the anesthetized rat after intra-intestinal administration. SP absorption was studied after intra-intestinal administration of the different kinds of tablets to anesthetized rats, by monitoring SP blood levels. SP and SD levels in the withdrawn samples from the dissolution systems and blood were analyzed by HPLC.
Results. The controlled erosion of the tablets resulted in equal release rates of SP and SD during the initial linear phase of the process. This synchronized release lasted over different time periods depending on the relative amount of HPMC in the formulations (from 1 hour to 4 hours for 2.5 and 17 % w/w of HPMC, respectively). The synchronous matrices increased SP bioavailability after intra-intestinal administration. The increase varied from 1.4 to 2.3-fold for the slow and the fast release formulations, respectively (compared with the nonsynchronous, SD containing control formulations), indicating the ability to control both erosion rate and length of intestinal segment in which absorption is taking place.
Conclusions. SP bioavailability after intestinal administration can be improved only if SP is released together with SD along the entire intestinal route. This can be accomplished by the design of synchronous matrices capable of concomitant release of SP and SD despite the differences in their water solubility. The ability to manipulate and control the duration of the synchronous phase of the matrices makes it possible for SP to be absorbed at different parts of the intestine. 相似文献