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1.
摘 要 目的: 建立HPLC波长切换法同时测定氨咖黄敏胶囊中4个成分的含量。方法: 采用Agilent ZORBAX SB C18色谱柱(250 mm×4.6 mm,5 μm),以乙腈(A)-甲醇(B)-磷酸二氢铵溶液(取0.1 mol·L-1磷酸二氢铵溶液1 000 ml ,加磷酸1 ml,混匀)(C)为流动相,梯度洗脱,流速1.0 ml·min-1,柱温 35℃,变换波长时间为(0~9 min :225 nm;9~38 min :450 nm)。结果: 采用HPLC波长切换法测定氨咖黄敏胶囊4个成分的含量,线性范围分别为:对乙酰氨基酚24.680~394.900 μg·ml-1(r=0.999 9),马来酸氯苯那敏0.201~3.214 μg·ml-1(r=0.999 9),咖啡因1.129~18.070 μg·ml-1(r=0.999 9),胆红素0.010~0.165 μg·ml-1(r=0.999 8);平均回收率分别为:99.25% (RSD=0.46%), 99.29% (RSD=0.32%),99.49% (RSD=0.48%)及99.75% (RSD=0.55%)(n=6)。结论:该法简单,灵敏,准确,重复性好,可用于氨咖黄敏胶囊的含量测定。 相似文献
2.
摘 要 目的: 提高黄连上清片质量标准,建立同时测定绿原酸、栀子苷、黄芩苷、盐酸小檗碱4种成分含量的方法。方法: 采用高效液相色谱法,色谱柱为DiamonsilTM C18(250 mm×4.6 mm,5 μm),流动相为二元梯度系统,其中溶剂A为乙腈,溶剂B为0.3%磷酸水溶液,流速为1.0 ml·min-1,检测波长为238 nm,柱温30℃,进样量为10 μl。结果: 绿原酸、栀子苷、黄芩苷、盐酸小檗碱的线性范围分别为8.11~81.10 μg·ml-1(r=0.999 7)、13.08~130.80 μg·ml-1(r=0.999 7)、10.76~107.60 μg·ml-1(r=0.999 8)、7.92~79.20 μg·ml-1(r=0.999 8)范围内呈良好的线性关系,平均加样回收率分别为99.19%(RSD=0.9%)、98.44%(RSD=1.1%)、99.12%(RSD=1.0%)、99.18%(RSD=1.1%)(n=9)。结论:该方法简便、准确、重复性好,可用于黄连上清片的质量控制。 相似文献
3.
摘 要 目的:建立HPLC CAD法测定硫酸卡那霉素注射液中卡那霉素和卡那霉素B含量的方法。方法: 采用Boston Green ODS C18(250 mm×4.6 mm,5 μm)色谱柱,以0.2 mol·L-1三氟醋酸溶液 甲醇(95 ∶〖KG-*2〗5)作为流动相,流速:1.0 ml·min-1,柱温:30℃,喷雾温度:55℃,喷雾压力:56.4 psi。结果:卡那霉素在0.385~38.500 μg·ml-1之间呈现良好的线性关系(r=0.999 9),检出限为0.075μg·ml-1,定量限为0.154 μg·ml-1,回收率为100.97%(n=9)。卡那霉素B在0.374~37.400 μg·ml-1之间呈现良好的线性关系(r=1.000 0),检出限为0.075 μg·ml-1,定量限为0.150 μg·ml-1,回收率为100.44%(n=9)。结论:建立的HPLC CAD测定卡那霉素和卡那霉素B含量的方法检出限低,操作简单准确,可以有效控制硫酸卡那霉素注射液的质量。 相似文献
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摘 要 目的:建立HPLC波长切换法同时测定茵栀祛黄胶囊中栀子苷、甘草苷、芦荟大黄素、大黄酸、大黄素、大黄酚和大黄素甲醚的含量。方法: 采用Waters Sunfire C18(250 mm×4.6 mm,5 μm)色谱柱;流动相:甲醇(A) 0.05%磷酸溶液(B)(梯度洗脱),流速为1.0 ml·min-1 ,柱温为25℃,检测波长(0~15 min:在238 nm波长下检测栀子苷和甘草苷;15~50 min:在254 nm波长下检测芦荟大黄素、大黄酸、大黄素、大黄酚和大黄素甲醚),进样量为10 μl。结果: 栀子苷、甘草苷、芦荟大黄素、大黄酸、大黄素、大黄酚和大黄素甲醚的线性范围分别为0.13~3.18 μg·ml-1(r=0.999 8)、0.19~4.84 μg·ml-1(r=0.999 7)、0.28~7.02 μg·ml-1(r=0.999 9)、0.13~3.16 μg·ml-1(r=0.999 9)、0.61~15.27 μg·ml-1(r=0.999 9)、0.32~8.03 μg·ml-1(r=0.999 9)、0.39~9.81 μg·ml-1(r=0.999 9);平均加样回收率分别为98.84%(RSD=0.74%)、99.34%(RSD=0.86%)、99.54%(RSD=0.30%)、99.56%(RSD=0.80%)、99.85%(RSD=0.41%)、99.57%(RSD=0.70%)、99.64%(RSD=0.30%)(n=9)。结论: 本文建立的HPLC含量测定方法,具有操作简便、专属性高、重复性良好、结果准确可靠的特点,可用于茵栀祛黄胶囊的质量控制。 相似文献
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摘 要 目的:建立多波长切换高效液相色谱法同时测定皮肤康洗液中芍药苷、甘草苷、甘草酸铵、大黄素和蛇床子素5种有效成分的含量。方法: 色谱柱为 Diamonsil C18柱(200 mm×4.6 mm,5 μm);流动相为乙腈 0.1%盐酸水溶液,梯度洗脱;流速为1.5 ml·min-1;柱温为30℃;检测波长为232 nm(0~6 min,检测芍药苷)、277 nm(6~10 min,检测甘草苷)、254 nm(10 min以后,检测甘草酸铵、大黄素、蛇床子素)。结果: 芍药苷、甘草苷、甘草酸铵、大黄素和蛇床子素的线性范围分别为28.20~282.0 μg·ml-1(r=0.999 7)、12.150~121.500 μg·ml-1(r=0.998 8)、13.420~134.200 μg·ml-1(r=0.999 5)、0.047~0.466 μg·ml-1(r=0.999 9)、2.380~23.800 μg·ml-1(r=0.999 9),平均加样回收率分别为98.49%,99.00%,98.38%,97.36%,97.70%,RSD分别为0.71%,0.62%,0.85%,0.92%,0.78%(n=6)。结论:该方法简便、准确、灵敏度高、重复性好,可用于同时测定皮肤康洗液中上述5个有效成分的含量。 相似文献
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摘 要 目的: 建立蒲药灌肠液中香蒲新苷、异鼠李素-3-O-新橙皮苷和延胡索乙素的HPLC含量测定方法。方法: 采用ZORBAX SB-C18色谱柱(250 mm×4.6 mm,5 μm),以乙腈-0.1%磷酸(三乙胺调节pH至6.0)为流动相,梯度洗脱程序,流速:1.0 ml·min-1,检测波长为254 nm(0~14 min)和281 nm(14~25 min),柱温:30 ℃。结果: 香蒲新苷、异鼠李素-3-O-新橙皮苷和延胡索乙素的线性范围分别为19.840~198.400 μg·ml-1(r=0.999 6)、20.520~205.200 μg·ml-1(r=0.999 8)和10.040~100.400 μg·ml-1(r=0.999 7),回收率分别为98.8%、98.6%和98.9%,RSD分别为1.4%、1.6%和1.3%(n=6)。结论: 该方法灵敏度高,专属性强,可用于蒲药灌肠液的质量控制。 相似文献
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摘 要 目的: 建立高效液相色谱法同时测定复方盐酸二甲双胍/沙格列汀渗透泵控释片的含量。方法: 采用UltimateXB-CN色谱柱(250 mm×4.6 mm,5 μm),流动相为0.01 mol·L-1磷酸二氢钾溶液(pH=4.7) 乙腈(15∶85),检测波长为208 nm,流速为1.0 ml·min-1,柱温为30℃,进样量20 μl。结果: 盐酸二甲双胍和沙格列汀分别在50.0~150.0 μg·ml-1和0.5~1.5 μg·ml-1浓度范围内与峰面积线性关系良好(r = 0.999 9和r=0.999 1)。平均回收率分别为99.5%和100.3%,RSD分别为0.56%和1.20%(n =9)。结论:该法专属性强,结果准确可靠,可用于复方盐酸二甲双胍/沙格列汀渗透控释片的质量控制。 相似文献
8.
摘 要 目的: 建立高效液相色谱法同时测定枳实消痞丸中芸香柚皮苷、柠檬苦素、和厚朴酚及厚朴酚含量的分析方法。方法: 色谱柱为岛津Shim-pack VP-ODS C18柱(250 mm×4.6 mm,5 μm),流动相A为乙腈 甲醇(1∶2),流动相B为水,梯度洗脱,流速为1.0 ml·min-1,柱温为30℃,进样量为20 μl;芸香柚皮苷检测波长为λ1=283 nm,柠檬苦素检测波长为λ2=210 nm,和厚朴酚、厚朴酚检测波长为λ3=294 nm。结果: 芸香柚皮苷、柠檬苦素、和厚朴酚、厚朴酚分别在5.26~105.20μg·ml-1(r=0.999 8)、7.65~153.00 μg·ml-1(r=0.999 4)、6.21~124.20 μg·ml-1(r=0.999 3)、6.45~129.00 μg·ml-1(r=0.999 6)范围内线性关系良好,平均加样回收率(n=6)分别为99.00%(RSD=0.77%)、98.17%(RSD=1.19%)、98.78%(RSD=0.86%)、97.90%(RSD=0.99%)。结论:该方法简便、准确、可靠,可用于枳实消痞丸的质量控制。 相似文献
9.
摘 要 目的: 建立HPLC法同时检测大败毒胶囊中绿原酸、咖啡酸和阿魏酸的测定方法。方法: 采用Agilent C18(250 mm×4.6 mm,5 μm)色谱柱;乙腈-0.4%磷酸溶液(15∶85)为流动相,流速:1.0 ml·min-1;检测波长:324 nm。柱温:30℃,进样量:10 μl。结果:线性范围:绿原酸5.42~32.43 μg·ml-1(r=0.999 8),咖啡酸2.63~26.32 μg·ml-1(r=0.999 4),阿魏酸1.44~14.44 μg·ml-1(r=0.999 9);平均回收率:绿原酸99.98%,RSD=0.2%(n=6),咖啡酸99.31%,RSD=0.4%(n=6),阿魏酸99.48%,RSD=0.8%(n=6)。结论:该方法操作简便、准确,重复性好,可用于测定大败毒胶囊中绿原酸、咖啡酸和阿魏酸的含量。 相似文献
10.
摘 要 目的:建立HPLC法同时测定玉真散中天麻素、升麻素苷、5-O-甲基维斯阿米醇苷、欧前胡素、异欧前胡素含量。方法: 色谱柱为Phenomenex Gemini C18柱(250 mm×4.6 mm,5 μm),柱温为25℃,流速为1.0 ml·min-1 ,流动相为甲醇 水梯度洗脱,检测波长为230 nm,进样量为5 μl。结果: 天麻素在2.68~214.00μg·ml-1范围内线性关系良好(r=0.999 9),平均回收率为100.2%,RSD为0.9%(n=6);升麻素苷在5.22~418.00 μg·ml-1范围内线性关系良好(r=0.999 7),平均回收率为99.9%,RSD为0.9%(n=6);5-O-甲基维斯阿米醇苷在4.57~365.80 μg·ml-1范围内线性关系良好(r=0.999 5),平均回收率为99.7%,RSD为1.0%(n=6);欧前胡素在5.22~417.20μg·ml-1范围内线性关系良好(r=0.999 6),平均回收率为99.0%,RSD为0.9%(n=6);异欧前胡素在5.29~423.20μg·ml-1范围内线性关系良好(r=0.999 8),平均回收率为100.2%,RSD为0.8%(n=6)。结论: 该方法简单、准确,可同时测定5种成分的含量,可用于玉真散的质量控制。 相似文献
11.
C D Klaassen 《European journal of pharmacology》1973,23(3):270-275
The choleretic properties of cholic, chenodeoxycholic, and deoxycholic acid and their taurine and glycine conjugates were compared to their ability to form micelles. It has previously been concluded that deoxycholate has the lowest critical micellar concentration; chenodeoxycholate is slightly higher and cholic is much higher. Conjugation with glycine and taurine has little or no effect on the critical micelle concentration. Since the choleretic properties of bile salts are thought to be directly proportional to their osmotic activities, one might suspect that deoxycholic acid would be the least choleretic, chenodeoxycholic slightly more choleretic and cholic much more choleretic, with little difference between the conjugated and unconjugated forms. However, in the present study, cholic, chenodeoxycholic and taurocholic acid produced similar increases in bile flow (450–700 μl/kg) after an equimolar dose (55 μM/kg). Except for the conjugation of deoxycholic acid with taurine, conjugation of these bile acids with glycine or taurine always decreased the choleretic properties of the bile acids. Therefore, it has been concluded that there is not a good correlation between the in vitro osmotic properties of bile acids and their ability to increase bile flow. 相似文献
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从产于四川宜宾翼梗五味子果实中,分得抗癌活性成分满五酸(1),同时分得schizandronic acid(2)和cuparenic acid(3),结构由与标准化合物比较光谱数据和物理常数而确证。 相似文献
13.
[摘要]目的:建立高效液相色谱法测定熊去氧胆酸片有关物质含量的方法。方法:用十八烷基硅烷键合硅胶为填充剂,以磷酸盐缓冲液(pH3.0)-甲醇-乙腈(35:37:28)为流动相,示差折光检测器测定。结果:在选定的色谱条件下,各成分可达到很好的分离,7-酮基胆石酸、胆酸、鹅去氧胆酸、胆石酸分别在0.05~2.00,0.05~2.00,0.05~2.00,0.10~2.00mg·mL-1的范围内线性关系良好(r>0.9989);精密度、稳定性试验的RSD均不大于0.5%;回收率分别为100.1%、99.8%、100.7%和101.9%(n=9,RSD<1.4%)。7-酮基胆石酸、胆酸、鹅去氧胆酸、胆石酸定量限分别为3.0、2.5、7.0、20μg·mL-1,检测限分别为0.8、0.7、2.0、3.5μg·mL-1。测定供试品两批,供试品一7-酮基胆石酸检出量为0.03%,胆酸和胆石酸均未检出,鹅去氧胆酸检出量为0.66%;供试品二7-酮基胆石酸、鹅去氧胆酸检出量均为0.10%,胆酸和胆石酸均未检出。结论:该方法灵敏度高,专属性强,适于测定熊去氧胆酸片中有关物质的含量。 相似文献
14.
Summary Four pure chemicals, ellagic acid (E), caffeic acid (C), luteolin (L) and punicic acid (P), all important components of the aqueous compartments or oily compartment of pomegranate fruit (Punica granatum), and each belonging to different representative chemical classes and showing known anticancer activities, were tested as potential inhibitors of in vitro invasion of human PC-3 prostate cancer cells in an assay employing Matrigel™ artificial membranes. All compounds significantly inhibited invasion when employed individually. When C, P, and L were equally combined at the same gross dosage (4 μ g/ml) as when the compounds were tested individually, a supradditive inhibition of invasion was observed, measured by the Kruskal-Wallis non-parametric test.An erratum to this article can be found at 相似文献
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目的建立快速测定升麻中咖啡酸、阿魏酸及异阿魏酸含量的HPLC方法。方法色谱柱为C18柱(4.6 mm×250 mm,5μm);流动相:乙腈-0.01%磷酸梯度洗脱;柱温:30℃;检测波长:320 nm;流速:1.0 ml/min。结果咖啡酸、阿魏酸、异阿魏酸分别在5.78~115.6μg/ml(r=0.9998,n=6),4.03~80.60μg/ml(r=1,n=6),16.45~329.00μg/ml(r=1,n=6)范围内线性关系良好,回收率在97.6%~101.5%。结论建立的方法准确可靠,可以用于测定升麻中咖啡酸、阿魏酸、异阿魏酸的含量。 相似文献
18.
Shi Yong Ryu Sang Un Choi Seung Ho Lee Chong Ock Lee Zaesung No Jong Woong Ahn 《Archives of pharmacal research》1994,17(5):375-377
Thirteen kinds of naturally occurring or derivatised triterpenes, reported to have an antitumoral property, were reinvestigated
on the basis of their direct cytotoxicity or the inhibitory activity on cell growth against five kinds of cultured human tumor
cells,i. e., A-549, SK-OV-3, SK-MEL-2, XF498 and HCT15,in vitro. Ursonic acidIII, betulinic acidVIII, betulonic acidX and glycyrrhetinic acidXI were exhibited a marked inhibition on cell growth. 相似文献
19.
Summary The antipyretic activity of three N-aryl-anthranilic acid derivatives, mefenamic acid, tolfenamic acid and flufenamic acid, was compared and their optimal antipyretic dose determined in a trial in 87 children (aged 5 months to 15 years), who suffered from infections and fever exceeding 38.5°C. Tolfenamic acid proved to be the most potent antipyretic agent of the three drugs; it was eight times more powerful than mefenamic acid and three times more powerful than flufenamic acid. The optimal antipyretic doses were: mefenamic acid 4 mg/kg, tolfenamic acid 0.5 mg/kg and flufenamic acid 1.5 mg/kg. It is evident that the antipyretic activity of these anthranilic acid derivatives is even greater than their antirheumatic effect, the difference being most noticeable in the case of tolfenamic acid. 相似文献
20.
目的建立L-天门冬氨酸中羟基丁二酸、丁二酸和富马酸等有关物质的检查方法。方法采用HPLC法,使用AQ C18色谱柱,0.01 mol.L-1的磷酸二氢钾水溶液(磷酸调pH3)为流动相,检测波长200 nm。结果主成分和杂质可完全分离。结论建立的方法灵敏、专属,可用于L-天门冬氨酸中有关物质的测定。 相似文献