推拿对坐骨神经损伤大鼠神经生长因子及其受体TrkA的影响

目的观察推拿对坐骨神经损伤大鼠行为学、神经生长因子(NGF)及其酪氨酸激酶受体(Tr-kA)的影响,探讨推拿治疗坐骨神经损伤的生物学机制。方法采用神经夹持损伤的方式建立大鼠坐骨神经损伤模型,通过斜板实验、热痛阈实验观察正常组、假手术组、模型组、对照组、推拿组大鼠的行为学变化;通过免疫组织化学染色观察各组大鼠脊髓内NGF及TrkA表达情况,进而统计分析各组间的差异。结果模型组和对照组大鼠行为学检测表明坐骨神经损伤后大鼠的运动及感觉功能明显降低(P<0.05),推拿治疗后斜板实验及热痛阈实验评分明显升高(P<0.05),并在治疗20 d后与正常组比无显著性差异(P>0.05);模型组、对照组及推拿组NGF及TrkA免疫组化表达与正常组相比均有显著性提高(P<0.05),推拿组NGF与TrkA表达与模型组相比也具有显著性差异(P<0.05)。结论推拿治疗可以通过提高机体神经生长因子NGF表达,提高NGF的高亲和力受体TrkA释放,从而抑制细胞抵抗凋亡,促进神经元存活,最终改善坐骨神经损伤大鼠的运动及感觉功能。     

基于马达蛋白探讨推拿对坐骨神经损伤大鼠轴浆运输功能的影响

目的 观察推拿对坐骨神经损伤大鼠轴浆运输马达蛋白表达改变的影响,探讨推拿治疗坐骨神经损伤的起效机制.方法 建立大鼠坐骨神经夹持损伤模型,通过腓肠肌温质量测量、热痛阈实验观察正常组、假手术组、模型组、模型对照组、推拿组大鼠的病理学、行为学变化;通过免疫组织化学染色观察各组大鼠脊髓内kinesin及dynein表达情况,并统计分析各组间的差异.结果 通过对模型组和模型对照组大鼠热痛阈、腓肠肌湿质量的检测,得出坐骨神经损伤后大鼠的感觉及运动功能明显降低的结果,在推拿治疗后,腓肠肌湿质量测量及热痛阈实验评分明显升高,并在治疗20 d后与正常组无显著性差异;模型组、对照组及推拿组kinesin及dynein免疫组化表达与正常组相比均有显著性提高,推拿组与模型组、对照组相比也具有显著性差异.结论 推拿治疗可以通过提高轴浆运输马达蛋白kinesin及dynein的表达,促进轴浆运输功能恢复,进而双向影响神经元细胞及神经支配靶组织,并最终改善坐骨神经损伤大鼠的运动及感觉功能.     

Expression of neurotrophin-3 and its receptors in lung tissues of rats with asthma

目的 研究神经营养素3 (NT-3)及其受体酪氨酸激酶C(TrkC)和p75神经营养素受体(p75 NTR)在哮喘大鼠肺组织中的表达情况.方法 将28只4～6周龄的SD大鼠随机分为分为哮喘组(n=16)(以卵蛋白致敏和激发建立大鼠哮喘模型)和正常对照组(n=12).取两组大鼠的肺组织制作病理切片,HE染色后光学显微镜下观察支气管和肺组织的病理学特征.采用Real-Time PCR技术检测两组大鼠肺组织中NT-3、TrkC和p75NTR mRNA的表达,并分析三者之间的相关性.结果 光学显微镜观察发现:哮喘组大鼠支气管壁周围有较多淋巴细胞和嗜酸性粒细胞浸润,可见支气管气道上皮脱落、杯状细胞增生及黏液栓增多等变化;正常对照组大鼠支气管及肺泡结构未见明显异常.哮喘组大鼠肺组织中NT-3、TrkC和p75NTR mRNA的相对表达量均显著高于正常对照组,差异有统计学意义(P＜0.05).相关性分析结果显示:NT-3 mRNA与TrkC mRNA的表达、NT-3mRNA与p75NTR mRNA的表达以及TrkC mRNA与p75NTR mRNA的表达均呈正相关(r分别为0.89、0.90和0.82,均P＜0.01).结论 致敏哮喘大鼠肺组织内NT-3 mRNA表达水平升高.NT-3通过上调TrkC和p75NTR mRNA的表达,增强NT-3的功能效应,三者共同参与了哮喘气道神经源性炎症的发生.     

神经营养素3及其受体在哮喘大鼠肺组织中的表达

目的研究神经营养素3(NT-3)及其受体酪氨酸激酶C(TrkC)和p75神经营养素受体(p75NTR)在哮喘大鼠肺组织中的表达情况。方法将28只4～6周龄的SD大鼠随机分为分为哮喘组(n=16)(以卵蛋白致敏和激发建立大鼠哮喘模型)和正常对照组(n=12)。取两组大鼠的肺组织制作病理切片,HE染色后光学显微镜下观察支气管和肺组织的病理学特征。采用Real-Time PCR技术检测两组大鼠肺组织中NT-3、TrkC和p75NTR mRNA的表达,并分析三者之间的相关性。结果光学显微镜观察发现:哮喘组大鼠支气管壁周围有较多淋巴细胞和嗜酸性粒细胞浸润,可见支气管气道上皮脱落、杯状细胞增生及黏液栓增多等变化;正常对照组大鼠支气管及肺泡结构未见明显异常。哮喘组大鼠肺组织中NT-3、TrkC和p75NTR mRNA的相对表达量均显著高于正常对照组,差异有统计学意义(P0.05)。相关性分析结果显示:NT-3 mRNA与TrkC mRNA的表达、NT-3mRNA与p75NTR mRNA的表达以及TrkC mRNA与p75NTR mRNA的表达均呈正相关(r分别为0.89、0.90和0.82,均P0.01)。结论致敏哮喘大鼠肺组织内NT-3 mRNA表达水平升高。NT-3通过上调TrkC和p75NTR mRNA的表达,增强NT-3的功能效应,三者共同参与了哮喘气道神经源性炎症的发生。     

推拿治疗坐骨神经损伤大鼠行为学研究

目的:从实验研究角度探寻推拿对周围神经损伤后所出现的感觉功能障碍的改善效应,并进一步揭示推拿对周围神经损伤后的中枢修复机制。方法:采用SD大鼠坐骨神经夹持损伤为模型,以"按摩手法模拟仪"于大鼠手术侧殷门穴、承山穴、阳陵泉穴施以点法、揉法,通过对大鼠甩尾热痛阈的分析,从行为学角度探寻推拿对坐骨神经损伤后的肢体感觉功能恢复的良性促进作用,为揭示推拿促进神经修复的机制提供理论支持。结果:经推拿治疗的坐骨神经损伤大鼠的50℃热水甩尾时间高于假手术组、模型组及模型对照组,且与模型组比较,差异显著,结果有统计学意义,说明推拿可以延长机体对热痛刺激的反应时间,进一步提示,推拿在坐骨神经损伤后可以有效地保护脊髓背角感觉神经元,并抑制神经损伤后所出现的神经源性疼痛。结论:本次研究以热痛刺激作为行为学观察指标,检测损伤神经修复后支配情况,结果表明,经推拿治疗后,可延长机体痛敏反应时间,说明推拿对坐骨神经损伤大鼠产生的疼痛感觉具有一定的抑制作用,其机理可能为保护了脊髓背角感觉神经元。     

推拿对坐骨神经损伤大鼠背根神经节降钙素基因相关肽表达的影响

目的:观察推拿对坐骨神经损伤后背根神经节神经元降钙素基因相关肽(calcitonin gene-related peptide,CGRP)表达的影响。方法:48只大鼠随机分为正常组、假手术组、模型组、模型对照组、推拿组,采用坐骨神经损伤(chronic constriction injury,SNI)模型,以按摩推拿手法模拟仪进行推拿手法干预,观察各组大鼠光热耐痛阈、痛敏分数变化及L3-5节段右侧背根神经节CGRP的表达情况。结果:造模7 d后,与同期正常组比较,模型组大鼠的耐痛阈值及痛敏分数均明显升高(P0.01);模型组CGRP在背根神经节的表达升高(P0.01)。在推拿治疗20次之后,与同期模型组比较,推拿组耐痛阈值及痛敏分数明显降低(P0.05);与同期模型组和模型对照组比较,推拿组CGRP在背根神经节的表达明显升高(P0.01)。结论:推拿可以提高周围神经损伤大鼠背根神经节内CGRP的表达,参与神经损伤修复过程,最终改善周围神经损伤大鼠的感觉功能。     

神经营养素3对坐骨神经损伤大鼠肌萎缩的修复作用

目的探讨神经营养素3(NT-3)对坐骨神经损伤大鼠肌萎缩的修复作用及其机制。方法 50只SD大鼠制成坐骨神经损伤模型,分为对照组和NT-3组,每组25只。显微镜下NT-3组右侧腓肠肌内注射NT-3质粒10μL(1 g.L-1),对照组注射生理盐水10μL。于注射后不同时间点观察2组大鼠caspase-3基因的表达情况、运动终板和肌细胞凋亡数目。结果坐骨神经损伤后大鼠caspase-3基因转录水平升高;NT-3组caspase-3基因转录水平和肌细胞凋亡率均低于对照组,差异有统计学意义(P<0.05);坐骨神经损伤后14、21 d NT-3组大鼠运动终板多于对照组,差异有统计学意义(P<0.05)。结论 NT-3抑制caspase-3基因的表达、肌细胞凋亡并提高运动终板数量,可能是促进神经纤维损伤后再生和减缓失神经肌萎缩的重要因素。     

远志对DPN大鼠坐骨神经Bcl-2和Bax蛋白表达的影响

目的:探讨远志对糖尿病周围神经病变(DPN)大鼠坐骨神经Bcl-2和Bax蛋白表达的影响.方法:Wistar大鼠随机分为正常组、DPN模型组和远志治疗组.DPN模型组和远志治疗组大鼠均建立DPN大鼠模型.DPN成模后,远志治疗组大鼠给予远志灌胃6周.采用HE染色法观察各组大鼠坐骨神经形态结构的变化,免疫组织化学染色法检测各组大鼠坐骨神经Bcl-2和Bax蛋白的表达情况.结果:与正常对照组大鼠比较,DPN模型组大鼠坐骨神经的形态结构发生明显病理变化;并且,Bcl-2蛋白的表达明显降低,Bax蛋白的表达明显升高(P＜0.05).与DPN模型组比较,远志治疗组大鼠坐骨神经的形态结构明显改善;并且,Bcl-2蛋白的表达明显升高,Bax蛋白的表达明显降低(P＜0.05).结论:远志可通过升高Bcl-2表达、降低Bax表达,抑制神经纤维变性,对DPN大鼠坐骨神经损伤具有保护作用.     

推拿对坐骨神经损伤模型大鼠神经超微结构的影响

目的 从超微病理学的角度探索推拿对坐骨神经损伤（Sciatic nerve injury,SNI）大鼠神经形态恢复的影响。方法 采用推拿手法模拟仪定性、定量模拟手法,对SNI模型大鼠进行干预,通过斜板试验和光热耐痛阈观察大鼠行为学改善情况,通过透射电镜观察并分析坐骨神经损伤局部轴索、髓鞘、雪旺细胞等超微结构的改变。结果 推拿治疗20次后,大鼠的斜板试验与光热耐痛阈结果与模型组相比均有显著差异（P<0.05）,且达到或接近正常组水平。正常组坐骨神经有髓神经纤维的髓鞘致密均匀,结构完整,形态规则,轴索无萎缩及肿胀,雪旺细胞丰富、正常;模型组髓鞘结构模糊、松散,出现空泡状变性,轴索萎缩或消失,偶见残存的线粒体,雪旺细胞线粒体空泡化,细胞趋于坏死;推拿治疗组髓鞘结构大多数趋完整态,空泡状缺损减少;轴索无明显肿胀,雪旺细胞部分空泡化或线粒体水肿。推拿治疗组有髓神经的髓鞘厚度和轴突直径与模型组比较,均有明显恢复,且逐渐接近正常组水平。结论 推拿可明显促进SNI大鼠坐骨神经纤维髓鞘的再生,轴索的恢复,减轻雪旺细胞胞质和线粒体的水肿,对周围神经损伤后超微结构的修复和再生有明显的促进作用。      

APPLICATION OF LORNOXICAM TO PATIENT-CONTROLLED ANALGESIA IN PATIENTS UNDERGOING ABDOMINAL SURGERIES

FOR anesthesiologis s ,treatingpostoperativepainhas alwaysbeen a problem.Althoughopioidshave been provedtobe effective,theirsideeffectscouldnotbeignored.With thedevelopmentofscienceand pharmacology,many drugs with aspectsof satisfactoryanalgesicefficacyand couldbe welltoleratedby patientshave been developed.And lornoxicamisone of them, which isa non-steroidalanti-inflammatorydrug (NSAID ), with analgesic, anti-infl-ammatory,andantipyreticproperties.Itseliminationhalf-time(3 to 5 hours) isle…     

Acupuncture Treatment of Vomiting

CASE HISTORY A female patient, 46 years old, head of the foreign affairs department of a certain university in Beijing, paid her first visit on October 9, 2006, with the chief complaint of vomiting for one month. She got vomiting after meals in early September. Before that, she had discomfortable sensation in the stomach due to angry with others, but she didn't pay much attention. Later, it developed into vomiting after eating. After the vomiting, the discomfort would be relieved, but with slight hypodynamia. She was once diagnosed as having 'neurogenic vomiting'. Having taken some western and Chinese drugs, the above symptoms were a little bit improved, but she would have nausea upon eating and with regurgitation. Because of the fear for vomiting, she did not dare to have food intake, with body weight reduction of 6 kilos in one month.     

Qiu Baoguo''''s Experience in Treating Consumptive Syndromes after Radio-Chemotherapies

Radiotherapy and chemotherapy are the important modern medical therapies for malignant tumors,yet they can also bring about serious local and systemic toxic side reactions so to decrease the patient;'s life quality,manifested by a series of consumptive symptoms.Having engaged in the combined work of Chinese and western medicine for nearly 50 years,the research fellow Qiu Baoguo in Henan Provincial Academy of TCM has developed his unique views on the TCM study of consumptive syndromes.The author of this essay had once the fortune tO follow Dr.Qiu in clinic,and specially would like to introduce in the following Dr.Qiu's experience in treating consumptive syndromes after radio-chemotherapies for patients with malignant tumor.     

Insomnia Due to Deficiency of Both the Heart and Spleen Treated by Acupuncture-Moxibustion and Chinese Tuina

OBJECTIVE: To observe therapeutic effects of the comprehensive therapy of acupuncture-moxibustion and Chinese Tuina for treatment of insomnia due to deficiency of both the heart and spleen. METHODS: 92 cases were divided randomly into the treatment group (treated by acupuncture-moxibustion and Chinese Tuina) and the control group (treated by acupuncture-moxibustion). RESULTS: The therapeutic effect of the treatment group was obviously superior to that of the control group (the CHI2 test showed P < 0.01). CONCLUSIONS: The comprehensive therapy of acupuncture-moxibustion and Chinese Tuina can give marked therapeutic effects for treatment of insomnia due to deficiency of both the heart and spleen.     

Analysis of CK-19,PDX-1,Nestin,Ngn3 in different donor islet purity in rats

Objective: To investigate if there are the CK-19, PDX-1, Nestin, Ngn3 positive cells in the donor islets of different purity in rats. Methods: Thirty male adult SD rats were randomly divided into 3 groups. Islets were isolated using digestion by ductal injection of collagenase. Group Ⅰ (n=10): Separating cell preparations were not purified, Group Ⅱ(n=10): Islet sediment was purified with 25% Ficoll400 ,Group Ⅲ (n=10): Islet sediment was purified with 25% and 11% Ficoll-400. The levels of protein of CK-19, PDX-1, Nestin and Ngn3 were detected by immunohistochemistry and the mRNA of CK-19, PDX-1, Nestin, Ngn3 was amplified by RT-PCR. Results: After two different purification methods applied, three islet preparations of different purities were obtained. The difference of islet purity was significant among various groups (P＜0.05). Compared with group Ⅱ and group Ⅲ,the protein and mRNA of CK-19, PDX-1, Nestin,Ngn3 were both higher in group Ⅰ; group Ⅲ was poorly expressed. Conclusions: The three different islet purity donor islet have different CK-19, PDX-1, Nestin, Ngn3 positive cells within them, indicating that there are some islet stem cells in the purified donor islet.     

Dr.Zhang Ren''''s Experience in the Acupuncture Treatment of Different Diseases with the Same Therapeutic Principle

Dr.Zhang Ren,the chief physician,is the chairman of Shanghai Acupuncture and Moxibustion Association.Having been engaged in medicine for about 40 years,he is experienced in treating various intractable diseases.In his long years of clinical practice,he advocates taking the TCM differentiation as the basis to seek for the acupuncture method for treatment of modern intractable diseases.The author of this essay had the fortune to follow Dr.Zhang in study.The following is a summary of Dr.Zhang's experience in the acupuncture treatment for different intractable diseases with the same therapeutic principle.     

Luo Lingjie''''s Experience in Treating Chronic Nephropathy

In treating chronic nephropathy,Luo Lingjie,a chief physician,pays attention to regulating the balance between yin and yang,treating infection if present,and removing pathogenic factors.He prescribes gentle drugs and uses carefully strongly warming-tonifying ones,emphasizes the importance of persuading the patient to persist in treatment with medication and nurse one's health for recuperation,and is good at combined use of TCM and western medicine therapy and brings the merits of various therapies into full play,with obvious theraoeutic effects.     

Acupuncture Combined with Spinal Tui Na for Treatment of Primary Dysmenorrhea in 30 Cases

Objective: To observe the therapeutic effects in acupunture treatment of primary dysmenorrhea combined with spinal Tui Na, and study its mechanism. Methods: Thirty cases of the treatment group were treated by acupuncture combined with spinal Tui Na, and thirty cases in the control group were treated by routine acupuncture. Results: The total effective rate was 93.3% in the treatment group, and 73.3% in the control group, with a significant difference between the two groups (P<0.05). Conclusions: Acupuncture combined with spinal Tui Na has good prospects for treatment of primary dysmenorrhea.