兔角膜缘上皮细胞培养联合羊膜行自体移植的实验研究

目的：用体外培养的角膜缘上皮细胞联合人羊膜的方法，行自体移植治疗角膜缘干细胞完全缺乏的兔眼。方法：制作10只兔右眼于细胞缺乏的模型，取其中7只兔的左眼上方角膜缘取一小块组织进行体外培养，并传代在无上皮细胞的人羊膜上。1个月后，手术切除10只兔受伤眼角膜表面被覆的新生血管膜和结膜上皮，7眼接受了含自体培养上皮细胞的羊膜移植，另3眼仅移植无上皮细胞的羊膜。结果：1周后原代培养的角膜缘上皮细胞融合，传代至无上皮细胞的人羊膜上继续生长2～3周，角膜上皮细胞可牢固的附着于羊膜上。移植了含有角膜上皮细胞的羊膜的兔子，术后早期都形成了角膜上皮化，并明显抑制了新生血管的再生，而接受羊膜移植的兔子，术后又出现明显的新生血管。结论：利用无上皮细胞的羊膜作为载体，培养角膜缘上皮细胞并自体移植可以恢复角膜上皮化、抑制新生血管生长。     

生物羊膜联合自体角膜缘移植术治疗眼表疾病

目的观察生物羊膜联合自体角膜缘移植术治疗眼表疾病的疗效。方法对33例(39只眼)不同类型眼表疾病患者行生物羊膜联合自体角膜缘移植,随访3～18个月。结果术后移植片全部存活,角膜上皮愈合。术后视力均有不同程度提高。随访期间,眼表稳定,无新生血管形成及复发。结论生物羊膜移植联合自体角膜缘移植术能有效治疗眼表疾病。     

培养角膜上皮和自体角膜缘移植治疗眼表损伤的比较

目的比较培养角膜上皮移植和自体角膜缘移植治疗严重眼表损伤的效果.方法对去除全角膜缘的新西兰大白兔行培养自体角膜上皮和自体角膜缘联合羊膜移植,观察术后角膜形态改变,并进行组织学和免疫细胞化学检测.结果培养角膜上皮移植后兔眼表面平整稳定,角膜中央无新生血管生长,自体角膜缘联合羊膜移植后兔眼表面不同程度结膜上皮化,两者治疗效果有显著性差异.结论培养角膜上皮移植能有效治疗以角膜缘干细胞受损为特征的严重眼表损伤.     

兔健眼角膜缘干细胞取材安全范围的实验研究

目的 确定自体角膜缘干细胞移植健眼取材的安全范围,为临床治疗提供实验依据.方法 兔眼角膜按30°、60°、90°等依次递增至210°分组切取角膜缘干细胞组织,术后不同时间点裂隙灯下动态观察每组角膜混浊、角膜上皮染色、新生血管3项临床指标,角膜上皮印迹细胞学检查及HE染色观察角膜上皮的细胞表型和病理学改变.结果 兔眼角膜缘切取范围大于150°时,出现角膜混浊,上皮持续缺损及新生血管长入,临床指标评分比较,差异有统计学意义(P＜0.01).印迹细胞学检查显示PAS阳性的结膜细胞.组织病理学可见角膜缘上皮细胞层次减少和新生血管.结论 自体角膜缘干细胞移植健眼的取材范围≤150°(相当于5个钟点位)是安全的.切取范围＞150°时角膜缘干细胞可能出现不可逆的失代偿.印迹细胞学检查是诊断角膜缘干细胞缺乏简单有效的方法.     

培养角膜上皮和自体角膜缘移植治疗眼表损伤的比较

目的比较培养角膜上皮移植和自体角膜缘移植治疗严重眼表损伤的效果。方法对去除全角膜缘的新西兰大白兔行培养自体角膜上皮和自体角膜缘联合羊膜移植，观察术后角膜形态改变，并进行组织学和免疫细胞化学检测。结果培养角膜上皮移植后兔眼表面平整稳定，角膜中央无新生血管生长，自体角膜缘联合羊膜移植后兔眼表面不同程度结膜上皮化，两者治疗效果有显著性差异。结论培养角膜上皮移植能有效治疗以角膜缘干细胞受损为特征的严重眼表损伤。     

保存人羊膜联合自体角膜缘移植治疗严重眼表疾病

目的：观察和评价羊膜移植在重建眼表面手术中的应用价值。方法：采用保存人羊膜联合自体或亲属健康眼的角膜缘移植治疗眼表面疾病４８例５２只眼。结果：术后随访６０～９２周,５２只眼中,４７只眼获得稳定的眼表面（除周边部有少量纤维血管翳外,中央部无新生血管及复发性上皮缺损发生）,３７只眼角膜透明或半透明,３１只眼脱盲。结论：羊膜移植不是上皮移植,而是改良基底的手术,以保存人羊膜为基底膜,自体或亲属健康眼的角膜缘为上皮细胞来源,重建眼表面治疗眼表面疾病,初步结果显示是一种有价值的方法,其作用机制尚不清楚。     

自体角膜缘干细胞移植和羊膜移植治疗翼状胬肉的疗效观察

目的观察自体角膜缘干细胞移植与羊膜移植治疗翼状胬肉术后临床效果。方法翼状胬肉86例（90眼）,随机分为两组。角膜缘干细胞组45例（47眼）行胬肉切除联合自体角膜缘干细胞移植;羊膜组41例（43眼）行胬肉切除联合羊膜移植。观察术后角膜愈合情况、羊膜上皮化、新生血管及胬肉复发情况。结果两种术式产生的不适症状时间相似。自体角膜缘干细胞移植后角膜创面愈合稍快于羊膜移植组。羊膜上皮化时间4～7 d。随访6个月至2年干细胞组胬肉复发2眼,羊膜组胬肉复发3眼。除复发病历外,手术区1个半月后均无明显新生血管生长。结论角膜缘干细胞移植及羊膜移植均有助于降低翼状胬肉术后复发率,是治疗翼状胬肉的良好术式。两种手术方式差异无统计学意义。     

新鲜羊膜联合自体角膜缘移植术治疗眼表疾病的疗效观察

【目的】观察新鲜羊膜联合自体角膜缘移植术治疗眼表疾病的疗效。【方法】对33例(39只眼)不同类型眼表疾病患行新鲜羊膜联合自体角膜缘移植，随访3～18m。【结果】术后移植片全部存活，角膜上皮愈合。术后视力均有不同程度提高。随访期问，眼表稳定，无新生血管形成及复发等。【结论】新鲜羊膜移植联合自体角膜缘移植术能有效治疗眼表疾病。     

保存羊膜移植重建兔眼表的形态学实验研究（摘要）

观察应用保存羊膜移植重建兔眼表后的形态变化 ,以期从结构上探讨羊膜移植后恢复正常眼表功能的机制 .方法 :3 0眼分六组 .A :正常眼 ;B :中央角膜板层切除 ;C :角膜缘部切除 ;D :中央角膜板层切除后行羊膜移植 ;E :角膜缘部切除后环行羊膜移植 ;F :结膜大部切除后行羊膜移植 .术后观察眼表的愈合情况 . 2月后取材行病理检查 .结果 :术后 2月 ,B组角膜透明 ,眼表恢复正常 ;C组角膜混浊 ,角膜表面结膜上皮化 ;D组羊膜被覆上皮细胞为角膜型上皮 ,羊膜内出现的角膜基质细胞 ,胶原纤维排列规则且分层 ,羊膜与其下基质愈合 ,羊膜变透明 ,羊膜…     

兔模型角膜缘移植的实验研究

目的　观察角膜缘移植的治疗效果 ,明确角膜表面重建的上皮来源 ,研究兔模型角膜缘移植排斥反应的免疫机制。方法　 2 0只健康白兔随机分为 3组 ,分别行角膜缘切除 角膜上皮剥离术、自体角膜缘移植术 (供体为对侧眼 )、异体角膜缘移植术 (供体为灰兔眼 )。术后以 1%荧光素钠染色观察角膜表面的上皮化 ,移植排斥反应通过移植眼的临床表现和病理检查加以评估。结果　单纯角膜缘切除 角膜上皮剥离眼术后角膜水肿、混浊 ,最终表面结膜上皮化、新生血管生成。自体角膜缘移植眼术后 11～ 14天 (平均 12 5天 ) ,角膜表面全部上皮化 ,角膜表面光滑、透明、规则 ,新生血管少。异体角膜缘移植组术后早期也可见角膜表面上皮化 ,术后 10～ 14天(平均 11 8天 )发生角膜上皮炎症反应 ,炎症反应分为 4个时期 :初发期、急性期、排斥期和慢性期 ;最后角膜上皮细胞被破坏、角膜混浊、新生血管生成。结论　对角膜缘组织和角膜上皮细胞完全被切除的眼表 ,通过角膜缘移植能重建角膜表面 ,而重建的角膜表面不仅能维持角膜组织的完整 ,还能减轻角膜的炎症 ,促进角膜基质新生血管的回退 ;可使临床更容易、准确地判断角膜缘移植排斥反应的发生及其强度。     

APPLICATION OF LORNOXICAM TO PATIENT-CONTROLLED ANALGESIA IN PATIENTS UNDERGOING ABDOMINAL SURGERIES

FOR anesthesiologis s ,treatingpostoperativepainhas alwaysbeen a problem.Althoughopioidshave been provedtobe effective,theirsideeffectscouldnotbeignored.With thedevelopmentofscienceand pharmacology,many drugs with aspectsof satisfactoryanalgesicefficacyand couldbe welltoleratedby patientshave been developed.And lornoxicamisone of them, which isa non-steroidalanti-inflammatorydrug (NSAID ), with analgesic, anti-infl-ammatory,andantipyreticproperties.Itseliminationhalf-time(3 to 5 hours) isle…     

Acupuncture Treatment of Vomiting

CASE HISTORY A female patient, 46 years old, head of the foreign affairs department of a certain university in Beijing, paid her first visit on October 9, 2006, with the chief complaint of vomiting for one month. She got vomiting after meals in early September. Before that, she had discomfortable sensation in the stomach due to angry with others, but she didn't pay much attention. Later, it developed into vomiting after eating. After the vomiting, the discomfort would be relieved, but with slight hypodynamia. She was once diagnosed as having 'neurogenic vomiting'. Having taken some western and Chinese drugs, the above symptoms were a little bit improved, but she would have nausea upon eating and with regurgitation. Because of the fear for vomiting, she did not dare to have food intake, with body weight reduction of 6 kilos in one month.     

Qiu Baoguo''''s Experience in Treating Consumptive Syndromes after Radio-Chemotherapies

Radiotherapy and chemotherapy are the important modern medical therapies for malignant tumors,yet they can also bring about serious local and systemic toxic side reactions so to decrease the patient;'s life quality,manifested by a series of consumptive symptoms.Having engaged in the combined work of Chinese and western medicine for nearly 50 years,the research fellow Qiu Baoguo in Henan Provincial Academy of TCM has developed his unique views on the TCM study of consumptive syndromes.The author of this essay had once the fortune tO follow Dr.Qiu in clinic,and specially would like to introduce in the following Dr.Qiu's experience in treating consumptive syndromes after radio-chemotherapies for patients with malignant tumor.     

Insomnia Due to Deficiency of Both the Heart and Spleen Treated by Acupuncture-Moxibustion and Chinese Tuina

OBJECTIVE: To observe therapeutic effects of the comprehensive therapy of acupuncture-moxibustion and Chinese Tuina for treatment of insomnia due to deficiency of both the heart and spleen. METHODS: 92 cases were divided randomly into the treatment group (treated by acupuncture-moxibustion and Chinese Tuina) and the control group (treated by acupuncture-moxibustion). RESULTS: The therapeutic effect of the treatment group was obviously superior to that of the control group (the CHI2 test showed P < 0.01). CONCLUSIONS: The comprehensive therapy of acupuncture-moxibustion and Chinese Tuina can give marked therapeutic effects for treatment of insomnia due to deficiency of both the heart and spleen.     

Analysis of CK-19,PDX-1,Nestin,Ngn3 in different donor islet purity in rats

Objective: To investigate if there are the CK-19, PDX-1, Nestin, Ngn3 positive cells in the donor islets of different purity in rats. Methods: Thirty male adult SD rats were randomly divided into 3 groups. Islets were isolated using digestion by ductal injection of collagenase. Group Ⅰ (n=10): Separating cell preparations were not purified, Group Ⅱ(n=10): Islet sediment was purified with 25% Ficoll400 ,Group Ⅲ (n=10): Islet sediment was purified with 25% and 11% Ficoll-400. The levels of protein of CK-19, PDX-1, Nestin and Ngn3 were detected by immunohistochemistry and the mRNA of CK-19, PDX-1, Nestin, Ngn3 was amplified by RT-PCR. Results: After two different purification methods applied, three islet preparations of different purities were obtained. The difference of islet purity was significant among various groups (P＜0.05). Compared with group Ⅱ and group Ⅲ,the protein and mRNA of CK-19, PDX-1, Nestin,Ngn3 were both higher in group Ⅰ; group Ⅲ was poorly expressed. Conclusions: The three different islet purity donor islet have different CK-19, PDX-1, Nestin, Ngn3 positive cells within them, indicating that there are some islet stem cells in the purified donor islet.     

Dr.Zhang Ren''''s Experience in the Acupuncture Treatment of Different Diseases with the Same Therapeutic Principle

Dr.Zhang Ren,the chief physician,is the chairman of Shanghai Acupuncture and Moxibustion Association.Having been engaged in medicine for about 40 years,he is experienced in treating various intractable diseases.In his long years of clinical practice,he advocates taking the TCM differentiation as the basis to seek for the acupuncture method for treatment of modern intractable diseases.The author of this essay had the fortune to follow Dr.Zhang in study.The following is a summary of Dr.Zhang's experience in the acupuncture treatment for different intractable diseases with the same therapeutic principle.     

Luo Lingjie''''s Experience in Treating Chronic Nephropathy

In treating chronic nephropathy,Luo Lingjie,a chief physician,pays attention to regulating the balance between yin and yang,treating infection if present,and removing pathogenic factors.He prescribes gentle drugs and uses carefully strongly warming-tonifying ones,emphasizes the importance of persuading the patient to persist in treatment with medication and nurse one's health for recuperation,and is good at combined use of TCM and western medicine therapy and brings the merits of various therapies into full play,with obvious theraoeutic effects.     

Acupuncture Combined with Spinal Tui Na for Treatment of Primary Dysmenorrhea in 30 Cases

Objective: To observe the therapeutic effects in acupunture treatment of primary dysmenorrhea combined with spinal Tui Na, and study its mechanism. Methods: Thirty cases of the treatment group were treated by acupuncture combined with spinal Tui Na, and thirty cases in the control group were treated by routine acupuncture. Results: The total effective rate was 93.3% in the treatment group, and 73.3% in the control group, with a significant difference between the two groups (P<0.05). Conclusions: Acupuncture combined with spinal Tui Na has good prospects for treatment of primary dysmenorrhea.