瘦素及胰岛素样生长因子-Ⅰ与卵泡发育的关系

目的：探讨卵泡液和血清中瘦素（Leptin）与胰岛素样生长因子-I（IGF-I）水平与卵泡发育的关系。方法：应用放射免疫法（RIA）检测67例患者取卵日血清及卵泡液中的Leptin与IGF-I水平。结果：（1）取卵日血清中的Leptin水平为（13．20±5．43）mg／L，显著高于卵泡液中kptin水平（10．86±5．73）mg／L，P〈0．05；卵泡液中的Leptin水平与血清中的Leptin水平呈正相关（r=0．84，P〈0．001）。血清中的IGF-I水平为（7．18±4．58）mg／L，显著高于卵泡液中IGF-I水平（4．36±3．03）mg／L（P〈0．05），卵泡液中的IGF-I水平与血清中的IGF-I水平呈正相关（r=0．79，P〈0．001）；（2）卵巢低反应型者的血清及卵泡液中Leptin水平显著高于卵巢中高反应型者，差异有显著性（P〈0．05）；（3）三种卵巢反应类型的血清及卵泡液中IGF—I水平比较，差异无显著性（P〉0．05）；（4）卵泡液及血清中的Leptin水平与IGF—I水平无明显的相关性（r=-0．079，P〉0．05）及（r=-0．127，P〉0．05）。结论：（1）卵泡液中的Leptin及IGF-I均来自血清；（2）高浓度的Leptin可能抑制卵泡发育，而低浓度的Leptin可能对卵泡发育没有影响；（3）体内IGF-I可能与其受体结合后发挥对卵泡发育的调节作用；（4）Leptin与IGF-I之间无明显相关关系。     

瘦素在超排卵周期的变化及对体外受精-胚胎移植结局的影响

目的 :探讨超排卵对瘦素水平的影响以及瘦素水平对体外受精 -胚胎移植 (IVF- ET)结局的影响。方法 :应用酶联免疫吸附法和放射免疫法分别测定 39例 IVF- ET患者在注 FSH、HCG前和取卵前的血清瘦素及排卵前卵泡的卵泡液瘦素、E2 水平。结果 :血清瘦素水平在注 HCG前 (2 6 .1± 2 .3)μg/ L明显高于注 FSH前 (9.3± 1.0 )μg/ L和取卵前 (15 .8± 2 .3) μg/ L,P均 <0 .0 0 1;卵泡液 (18.5± 2 .2 ) μg/ L和同期血清 (15 .8± 2 .3) μg/ L的瘦素水平比较 ,经 t检验无显著性差异 ;妊娠组的卵泡液和同期血清瘦素水平 (11.7± 1.8) μg/ L和 (8.7± 1.5 ) μg/ L明显低于非妊娠组的卵泡液和同期血清瘦素水平 (2 0 .8± 2 .7) μg/ L和 (18.3± 2 .9) μg/ L,P<0 .0 5。结论 :超排卵后瘦素浓度过高可能不利于卵泡的生长和发育。     

人卵泡液TNF-α和IL-6的水平及其对卵巢功能的影响

目的　测定卵泡液中肿瘤坏死因子 -α(TNF-α)和白细胞介素 - 6 (IL- 6 )水平 ,并探讨其与雌二醇 (E2 )产生及卵母细胞成熟度的关系 .方法　酶联免疫法测定 97份卵胞液及2 6份血清标本的 TNF- α和 IL- 6水平 ,放射免疫法测定 E2 水平 ,并记录每份卵泡液中卵母细胞的成熟度 .结果　卵泡液中 TNF- α和 IL- 6水平分别为 (2 6 .5± 7.2 ) ng·L- 1和 (32 8.0± 48.7) ng· L- 1 ,分别较血清中 TNF-α和 IL - 6的水平高 9倍和 2 0倍 (P<0 .0 1) ;卵泡液 TNF- α和 IL- 6水平与卵泡液中 E2 水平及卵母细胞成熟度呈正相关 (r值为 0 .6 48和 0 .6 92 ,P<0 .0 1) .结论　卵泡液中存在较高水平的 TNF-α和IL- 6 ,其与 E2 产生及卵母细胞成熟度有一定关系     

白细胞介素6及肿瘤坏死因子-α对体外受精-胚胎移植结局的影响

目的 :探讨在体外受精 -胚胎移植 (IVF -ET)过程中血清及卵泡液中的白细胞介素 -6(IL -6)、肿瘤坏死因子 -α(TNF -α)水平与卵泡发育、卵巢内分泌、激素反应类型、年龄、胚胎质量及妊娠之间的关系。方法 :用放射免疫方法检测了 98例患者 (共 10 2周期 )卵泡早期、卵泡中期和取卵日血清中的IL -6、TNF -α水平及取卵日卵泡液中的IL -6、TNF -α水平。结果 :⑴在超促排卵周期中 ,血清中的IL -6水平及IL -6/TNF -α比值随着卵泡的逐渐发育成熟而逐渐升高 ,但血清TNF -α的水平则随着卵泡发育进程而逐渐降低 ,各组间存在极显著性差异 (P均 <0 .0 0 1) ;⑵取卵日卵泡液中的IL -6、TNF -α水平显著高于血清中上述细胞因子的水平 (P <0 .0 0 1) ,⑶随着获卵数的增加 ,卵泡液中IL -6、TNF -α水平显著降低 (P均 <0 .0 0 1) ;而卵泡液IL -6/TNF -α比值则随着获卵数的增加显著升高 (P均<0 .0 5) ;⑷年龄≤ 3 0岁组卵泡液中的IL -6水平小于年龄 >3 1岁组 (P <0 .0 5) ,年龄 3 1～ 3 5岁组与年龄 >3 5岁组之间的卵泡液IL -6水平无显著性差异 (P >0 .0 5) ,不同年龄组间卵泡液TNF -α水平及IL -6/TNF -α比值均无显著性差异 (P >0 .0 5) ;⑸高质量胚胎比≥ 0 .5组的卵泡液IL -6、TNF -α浓度较 <0 .5组显著降低 (P     

瘦素及胰岛素样生长因子-Ⅰ与卵泡发育的关系

目的:探讨卵泡液和血清中瘦素(Leptin)与胰岛素样生长因子-Ⅰ(IGF-Ⅰ)水平与卵泡发育的关系.方法:应用放射免疫法(RIA)检测67例患者取卵日血清及卵泡液中的Leptin与IGF-Ⅰ水平.结果:(1)取卵日血清中的Leptin水平为(13.20±5.43)mg/L,显著高于卵泡液中Leptin水平(10.86±5.73)mg/L,P＜0.05;卵泡液中的Leptin水平与血清中的Leptin水平呈正相关(r=0.84,P＜0.001).血清中的IGF-I水平为(7.18±4.58)mg/L,显著高于卵泡液中IGF-I水平(4.36±3.03)mg/L(P＜0.05),卵泡液中的IGF-Ⅰ水平与血清中的IGF-Ⅰ水平呈正相关(r=0.79,P＜0.001);(2)卵巢低反应型者的血清及卵泡液中Leptin水平显著高于卵巢中高反应型者,差异有显著性(P＜0.05);(3)三种卵巢反应类型的血清及卵泡液中IGF-Ⅰ水平比较,差异无显著性(P＞0.05);(4)卵泡液及血清中的Leptin水平与IGF-Ⅰ水平无明显的相关性(r=-0.079,P＞0.05)及(r=-0.127,P＞0.05).结论:(1)卵泡液中的Leptin及IGF-Ⅰ均来自血清;(2)高浓度的Leptin可能抑制卵泡发育,而低浓度的Leptin可能对卵泡发育没有影响;(3)体内IGF-Ⅰ可能与其受体结合后发挥对卵泡发育的调节作用;(4)Leptin与IGF-Ⅰ之间无明显相关关系.     

醒脑静注射液对脑缺血-再灌注损伤家兔血清白介素-8的影响

目的 :观察醒脑静注射液 (XNJI)对脑缺血 再灌注损伤 (CIRI)家兔血清白介素 8(IL 8)水平的影响 ,并探讨其脑保护机制。方法 :2 8只家兔随机分为假手术对照组 (C组 )、缺血再灌注组 (I R组 )和醒脑静注射液组 (XNJI组 ) ,分别在缺血前、缺血 3 0min、再灌注 3 0min、60min、12 0min时取静脉血 ,测定血清IL 8浓度 ,实验结束后取脑组织观察脑超微结构的变化。结果 :脑缺血 再灌注期间 ,I R组不同时点血清IL 8水平明显高于XNJI组 ,缺血 3 0min为 (0 .62± 0 .18)ng·L-1及 (0 .43± 0 .0 8)ng·L-1,P <0 .0 5 ;再灌注 3 0min ,为血清IL 8水平 (0 .73± 0 .19)ng·L-1及 (0 .45± 0 .0 9)ng·L-1;再灌注 60min ,血清IL 8水平为 (0 .87± 0 .2 9)ng·L-1及 (0 .47± 0 .0 6)ng·L-1;再灌注 12 0min血清IL 8水平为 (1.0 3± 0 .43 )ng·L-1及 (0 .44± 0 .0 7)ng·L-1,再灌注各时点组均P <0 .0 1,超微结构发生异常改变 ;XNJI组不同时点血清IL 8浓度显著低于I R组 (XNJI组缺血 3 0min为(0 .48± 0 .0 7)ng·L-1,P <0 .0 5 ;再灌注 3 0min为 (0 .5 0± 0 .0 8)ng·L-1;再灌注 60min为 (0 .5 5± 0 .14)ng·L-1;再灌注 12 0min为 (0 .5 9± 0 .17)ng·L-1,再灌注各时点组P <0 .0 1) ,其超微结构异常改变较I R组     

正常月经周期中血清瘦素与雌激素的关系

目的 :探索健康妇女正常月经周期中血清瘦素与雌激素的关系 .方法 :①对 18例月经规则妇女 (2 8± 1)d ,用放射免疫法分别在其月经第 1,7,14和 2 1d测定血清瘦素及性激素 (FSH ,E2 ,PRO ,T) .②对 19例不孕妇女 ,在受精前用FSH 2 2 5IU·d-1治疗后第 3d、第 5d用放免法测定其血清瘦素及雌激素浓度 .结果 :①血清瘦素在月经周期中的生理性变化 ,月经第 1d ,血清瘦素为 (5 .4 9± 1.0 8) μg·L-1,随着卵泡的发育成熟 ,瘦素水平逐渐升高 ,14d达高峰 (9.2 8± 1.87) μg·L-1,其差异有显著性 (P <0 .0 1) .②用FSH治疗 3,5d ,血清雌激素浓度和瘦素水平 [(89.0 8± 18.0 7)ng·L-1,(10 .99± 1.94 ) μg·L-13rdday],[(12 6 .2 6± 19.79)ng·L-1,(14 .78± 2 .0 0 ) μg·L-15thday]均较治疗前 [(5 8.5 9± 6 .4 0 )ng·L-1,(5 .4 6± 1.16 ) μg·L-1]明显升高 (P <0 .0 1) .结论 :月经周期中血清瘦素和雌激素的变化一致 ,雌激素参与瘦素分泌的调节或瘦素的分泌调节与雌激素有关     

超排卵时卵巢对促性腺激素的反应与颗粒细胞促卵泡激素受体的表达

目的 :探讨促排卵周期卵巢对促性腺激素反应性与颗粒细胞促卵泡激素受体 (FSHR)表达的关系。方法 :根据取卵时卵泡发育数不同 ,将卵巢对促排卵药物的反应分为低反应型 (卵泡数≤ 3个 )、中反应型 (卵泡数 4～13个 )和高反应型 (卵泡数≥ 14个 ) ,分别为 11例、15例和 13例。采用逆转录聚合酶链反应 (RT- PCR)测定 3种反应类型的卵泡颗粒细胞 FSHR m RNA的表达量。结果 :低反应型 FSHR m RNA表达量显著低于中、高反应型 (相对积分值分别为 0 .5 4± 0 .0 7、0 .90± 0 .17和 1.2 0± 0 .4 5 ,P<0 .0 5 )。结论 :卵巢对促性腺激素的反应与颗粒细胞FSHR m RNA的表达量高低有关。     

急性冠脉综合征患者血清白介素1,6,10的变化

目的 :观察血清中炎症性细胞因子白介素 (IL) 1 β,IL 6及抗炎细胞因子IL 1 0含量在急性冠脉事件、稳定性冠脉事件及健康对照之间是否存在差异 .方法 :经冠状动脉造影确诊的急性冠脉综合征 (ACS)患者 1 0 0例及我院健康志愿者 1 2 0例作为观察对象 ,ACS患者症状稳定 4mo后对其进行随访 .血清IL 1 β,IL 6及IL 1 0含量测定用免疫测定法 .结果 :血清IL 1 β浓度在ACS患者发病时和稳定期高于健康对照 (1 6 0± 2 7vs 84± 2 5ng·L -1 ,P <0 .0 5 ;1 2 4± 2 9vs 84±2 5ng·L -1 ,P <0 .0 5 ) ;血清IL 6浓度在ACS患者发病时和自身稳定期及健康对照之间无显著性差异 ;血清IL 1 0浓度在ACS患者发病时低于自身稳定期和健康对照组 (1 4± 6vs2 5± 8ng·L -1 ,P <0 .0 5 ;1 4± 6vs 2 9± 7ng·L -1 ,P <0 .0 5 ) .结论 :IL 1 β在冠状动脉粥样硬化发病中起一定作用 ;IL 1 0对于AS斑块稳定性起保护作用 ,且其血清中低浓度表达可作为斑块不稳定的一个预测指标     

VF超促周期妇女血清及卵泡液中血管

目的检测血管内皮生长因子(VEGF)在体外受精-胚胎移植(IVF-ET)超促排卵周期中不孕妇女血清及卵泡液中的水平。方法收集IVF治疗的187个因输卵管因素年龄24～38岁的不孕妇女在促性腺激素(Gn)注射日、人绒毛膜促性腺激素(HCG)注射日的血清和卵泡液,用酶联免疫方法测定VEGF在血清和卵泡液中的水平。结果 HCG日血清VEGF的水平明显高于Gn启动日(135.36±1.87)ng/L与(70.34±1.33)ng/L(P<0.01);随着年龄的增长卵泡液VEGF的水平呈上升趋势(r=0.493,P<0.01)两者呈正相关;获卵数≥20个患者单个卵泡中VEGF的水平低于获卵数≤5个的患者(1299.63±43.70)ng/L与(2460.23±548.45)ng/L,但差异无统计学意义(P>0.05)。结论随着促排卵过程中卵泡的发育,血清中VEGF的水平明显增多;随着年龄的增加VEGF在卵泡液中的水平明显升高。     

APPLICATION OF LORNOXICAM TO PATIENT-CONTROLLED ANALGESIA IN PATIENTS UNDERGOING ABDOMINAL SURGERIES

FOR anesthesiologis s ,treatingpostoperativepainhas alwaysbeen a problem.Althoughopioidshave been provedtobe effective,theirsideeffectscouldnotbeignored.With thedevelopmentofscienceand pharmacology,many drugs with aspectsof satisfactoryanalgesicefficacyand couldbe welltoleratedby patientshave been developed.And lornoxicamisone of them, which isa non-steroidalanti-inflammatorydrug (NSAID ), with analgesic, anti-infl-ammatory,andantipyreticproperties.Itseliminationhalf-time(3 to 5 hours) isle…     

Acupuncture Treatment of Vomiting

CASE HISTORY A female patient, 46 years old, head of the foreign affairs department of a certain university in Beijing, paid her first visit on October 9, 2006, with the chief complaint of vomiting for one month. She got vomiting after meals in early September. Before that, she had discomfortable sensation in the stomach due to angry with others, but she didn't pay much attention. Later, it developed into vomiting after eating. After the vomiting, the discomfort would be relieved, but with slight hypodynamia. She was once diagnosed as having 'neurogenic vomiting'. Having taken some western and Chinese drugs, the above symptoms were a little bit improved, but she would have nausea upon eating and with regurgitation. Because of the fear for vomiting, she did not dare to have food intake, with body weight reduction of 6 kilos in one month.     

Qiu Baoguo''''s Experience in Treating Consumptive Syndromes after Radio-Chemotherapies

Radiotherapy and chemotherapy are the important modern medical therapies for malignant tumors,yet they can also bring about serious local and systemic toxic side reactions so to decrease the patient;'s life quality,manifested by a series of consumptive symptoms.Having engaged in the combined work of Chinese and western medicine for nearly 50 years,the research fellow Qiu Baoguo in Henan Provincial Academy of TCM has developed his unique views on the TCM study of consumptive syndromes.The author of this essay had once the fortune tO follow Dr.Qiu in clinic,and specially would like to introduce in the following Dr.Qiu's experience in treating consumptive syndromes after radio-chemotherapies for patients with malignant tumor.     

Insomnia Due to Deficiency of Both the Heart and Spleen Treated by Acupuncture-Moxibustion and Chinese Tuina

OBJECTIVE: To observe therapeutic effects of the comprehensive therapy of acupuncture-moxibustion and Chinese Tuina for treatment of insomnia due to deficiency of both the heart and spleen. METHODS: 92 cases were divided randomly into the treatment group (treated by acupuncture-moxibustion and Chinese Tuina) and the control group (treated by acupuncture-moxibustion). RESULTS: The therapeutic effect of the treatment group was obviously superior to that of the control group (the CHI2 test showed P < 0.01). CONCLUSIONS: The comprehensive therapy of acupuncture-moxibustion and Chinese Tuina can give marked therapeutic effects for treatment of insomnia due to deficiency of both the heart and spleen.     

Analysis of CK-19,PDX-1,Nestin,Ngn3 in different donor islet purity in rats

Objective: To investigate if there are the CK-19, PDX-1, Nestin, Ngn3 positive cells in the donor islets of different purity in rats. Methods: Thirty male adult SD rats were randomly divided into 3 groups. Islets were isolated using digestion by ductal injection of collagenase. Group Ⅰ (n=10): Separating cell preparations were not purified, Group Ⅱ(n=10): Islet sediment was purified with 25% Ficoll400 ,Group Ⅲ (n=10): Islet sediment was purified with 25% and 11% Ficoll-400. The levels of protein of CK-19, PDX-1, Nestin and Ngn3 were detected by immunohistochemistry and the mRNA of CK-19, PDX-1, Nestin, Ngn3 was amplified by RT-PCR. Results: After two different purification methods applied, three islet preparations of different purities were obtained. The difference of islet purity was significant among various groups (P＜0.05). Compared with group Ⅱ and group Ⅲ,the protein and mRNA of CK-19, PDX-1, Nestin,Ngn3 were both higher in group Ⅰ; group Ⅲ was poorly expressed. Conclusions: The three different islet purity donor islet have different CK-19, PDX-1, Nestin, Ngn3 positive cells within them, indicating that there are some islet stem cells in the purified donor islet.     

Dr.Zhang Ren''''s Experience in the Acupuncture Treatment of Different Diseases with the Same Therapeutic Principle

Dr.Zhang Ren,the chief physician,is the chairman of Shanghai Acupuncture and Moxibustion Association.Having been engaged in medicine for about 40 years,he is experienced in treating various intractable diseases.In his long years of clinical practice,he advocates taking the TCM differentiation as the basis to seek for the acupuncture method for treatment of modern intractable diseases.The author of this essay had the fortune to follow Dr.Zhang in study.The following is a summary of Dr.Zhang's experience in the acupuncture treatment for different intractable diseases with the same therapeutic principle.     

Luo Lingjie''''s Experience in Treating Chronic Nephropathy

In treating chronic nephropathy,Luo Lingjie,a chief physician,pays attention to regulating the balance between yin and yang,treating infection if present,and removing pathogenic factors.He prescribes gentle drugs and uses carefully strongly warming-tonifying ones,emphasizes the importance of persuading the patient to persist in treatment with medication and nurse one's health for recuperation,and is good at combined use of TCM and western medicine therapy and brings the merits of various therapies into full play,with obvious theraoeutic effects.     

Acupuncture Combined with Spinal Tui Na for Treatment of Primary Dysmenorrhea in 30 Cases

Objective: To observe the therapeutic effects in acupunture treatment of primary dysmenorrhea combined with spinal Tui Na, and study its mechanism. Methods: Thirty cases of the treatment group were treated by acupuncture combined with spinal Tui Na, and thirty cases in the control group were treated by routine acupuncture. Results: The total effective rate was 93.3% in the treatment group, and 73.3% in the control group, with a significant difference between the two groups (P<0.05). Conclusions: Acupuncture combined with spinal Tui Na has good prospects for treatment of primary dysmenorrhea.