S-100B在新生儿缺氧缺血性脑病中的临床应用探讨

目的:研究新生儿缺氧缺血性脑病(HIE)患儿脐血、血清、尿S-100B蛋白水平,探讨其在缺氧缺血性脑病早期诊断中的价值。方法:采用酶联免疫吸附法(ELISA)检测60例缺氧缺血性脑病患儿及50例健康足月新生儿脐血、血清、尿S-100B蛋白水平。结果:(1)HIE组脐血、血清、尿S-100B蛋白水平明显高于对照组。(2)轻、中重度HIE患儿的脐血、血清、尿S-100B蛋白水平明显高于对照组。(3)尿S-100B蛋白水平在出生后48h之内逐渐升高,之后下降。结论:脐血、血清、尿S-100B均可作为新生儿缺氧缺血性脑病早期预测的指标。脐血、尿标本更易获取,对患儿损伤更小。     

血清S100b蛋白在新生儿缺氧缺血性脑病早期含量变化及其临床意义

目的:探讨血清S100b蛋白对新生儿缺氧缺血性脑病(HIE)早期诊断的临床意义.方法: 检测65例HIE患儿在出生后24h、72h血清S100b蛋白的含量,并与同期50例正常新生儿血清S100b蛋白含量相比较.结果:HIE组在出生24h、72h血清S100b蛋白含量明显高于对照组,差异有显著意义(P＜0.01);轻度HIE组与中度HIE组在出生24h、72h血清S100b蛋白含量差异有显著意义(P＜0.01),中度HIE组与重度HIE组在出生24h、72h血清S100b蛋白含量差异有显著意义(P＜0.01),血清S100b蛋白含量与HIE脑损伤程度呈正相关.结论:早期检测HIE患儿血清中S100b蛋白含量对HIE早期诊断及病情程度判定具有一定应用价值.     

S-100B在新生儿缺氧缺血性脑病中的临床应用探讨

目的研究新生儿缺氧缺血性脑病（HIE）患儿脐血、血清、尿S-100B蛋白水平,探讨其在缺氧缺血性脑病早期诊断中的价值。方法采用酶联免疫吸附法（ELISA）检测60例缺氧缺血性脑病患儿及50例健康足月新生儿脐血、血清、尿S-100B蛋白水平。结果HIE组脐血、血清、尿S-100B蛋白水平明显高于对照组;轻、中重度HIE患儿的脐血、血清、尿S-100B蛋白水平明显高于对照组;尿S-100B蛋白水平在出生后48h之内逐渐升高,之后下降。结论脐血、血清、尿S-100B均可作为新生儿缺氧缺血性脑病早期预测的指标。脐血、尿标本更易获取,对患儿损伤更小。     

sLOX-1、激活素A和S100β蛋白水平与新生儿缺氧缺血性脑病患儿病情严重程度的相关性

目的:分析可溶性凝集素样氧化低密度脂蛋白受体-1(sLOX-1)、激活素A(ACTA)和S100β蛋白水平与新生儿缺氧缺血性脑病患儿病情严重程度的相关性。方法:回顾性分析2013年4月至2019年8月该院收治的48例新生儿缺氧缺血性脑病患儿的临床资料,设为新生儿缺氧缺血性脑病组,另选取同期该院48名健康新生儿为对照组。比较两组血清sLOX-1、ACTA和S100β蛋白水平,以及不同严重程度新生儿缺氧缺血性脑病患儿血清sLOX-1、ACTA和S100β蛋白水平;采用Pearson相关性分析法分析sLOX-1、ACTA和S100β蛋白水平与新生儿缺氧缺血性脑病严重程度的相关性。结果:新生儿缺氧缺血性脑病组血清sLOX-1、ACTA和S100β蛋白水平均高于对照组,差异有统计学意义(P<0.05);重度新生儿缺氧缺血性脑病患儿血清sLOX-1、ACTA和S100β蛋白水平高于中度、轻度新生儿缺氧缺血性脑病患儿,且中度新生儿缺氧缺血性脑病患儿高于轻度新生儿缺氧缺血性脑病患儿,差异有统计学意义(P<0.05);血清sLOX-1、ACTA和S100β蛋白水平与新生儿缺氧缺血性脑病患儿病情严重程度呈正相关(r>0,P<0.05)。结论:血清sLOX-1、ACTA和S100β蛋白水平与新生儿缺氧缺血性脑病患儿病情严重程度呈正相关。     

新生儿HIE血清S100B蛋白和NSE浓度变化及临床意义的研究

目的了解新生儿缺氧缺血性脑病(HIE)患儿血清S100B蛋白和神经元特异性烯醇化酶(NSE)的浓度变化及临床意义。方法 使用酶联免疫吸附试验法测定50例足月HIE患儿生后第1、3、7天时的血清S100B蛋白和NSE浓度;选择同期出生60例无窒息史、无神经系统疾病史的足月新生儿作为对照组。结果 HIE组血清S100B蛋白和NSE含量明显高于对照组(P<0.05);各个检测时段HIE组血清S100B蛋白和NSE含量呈正相关(P<0.05);如同时检测第3天血清S100B蛋白和NSE值,可显著提高敏感度和特异度,分别为97.4%和95.1%,较单独应用两种方法敏感度和特异度均提高。结论 HIE患儿血清S100B蛋白和NSE浓度增高,可作为HIE诊断的补充依据。     

血清S100B蛋白和神经元特异性烯醇化酶在新生儿缺氧缺血性脑病早期诊断中的应用价值

目的：探讨血清 S100B蛋白和神经元特异性烯醇化酶（NSE）在新生儿缺氧缺血性脑病（HIE）早期诊断及判断预后的应用价值。方法选取2010年1月至2014年12月深圳市儿童医院新生儿科住院的 HIE的患儿和健康新生儿作为研究对象。健康对照组40例,HIE组97例,其中轻度组41例,中度组30例,重度组26例。入组的新生儿均于出生后的第1、3、7天晨起空腹时采集静脉血测定血清S100蛋白和 NSE 含量。结果 HIE 组患儿各个时间点 S100B 蛋白和 NSE 含量均明显高于健康对照组（P均＜0.05）。治疗前中、重度组血清 S100B蛋白和 NSE含量明显高于轻度组（P ＜0.05）经治疗后血清 S100B蛋白和 NSE含量均明显下降（P均＜0.05）。血清 S100B蛋白含量与 NSE含量呈正相关（P ＜0.05）。结论血清 S100B蛋白和 NSE在 HIE的早期诊断、损伤程度及预后的判断有重要意义。     

新生儿缺氧缺血性脑病血清酸性钙结合蛋白S-100的初步研究

目的：研究新生儿缺氧缺血性脑病（HIE）患儿血清酸性钙结合蛋白S－100水平及其临床意义。方法：采用发光免疫法检测52例HIE患儿及15例正常新生儿血清S－100水平，结果：中，重度HIE患儿急性期血清S－100水平明显高于对照组，并与病情严重程度呈正相关，结论：新生儿HIE患儿血清S－100水平可作为HIE的早期诊断及判断预后的有效指标。     

S-100蛋白在新生儿缺氧缺血性脑病中脑损伤的诊断价值

目的分析S-100蛋白在新生儿缺氧缺血性脑病中脑损伤的诊断价值。方法选取本院收治的80例缺氧缺血性脑病中脑损伤患儿作为观察组,再选取健康新生儿80例作为对照组,所有受检者的收取时间(2016年3月至2018年3月),对受检者S-100蛋白进行检测,将两组受检者的S-100蛋白指标进行对比。结果观察组缺氧缺血性脑病中脑损伤患儿的S-100蛋白指标(3.72±1.61)ng/m L高于对照组健康新生儿S-100蛋白指标(0.10±0.01)ng/m L(P0.05)。结论 S-100蛋白在新生儿缺氧缺血性脑病中脑损伤的诊断具有较高的价值,能为缺氧缺血性脑病中脑损伤患儿早期治疗提供有利依据,值得进一步应用。     

新生儿缺氧缺血性脑损伤血清神经元特异性烯醇化酶的变化及临床意义

目的:探讨血清神经元特异性烯醇化酶(NES)在新生儿缺氧缺血性脑损伤中的临床意义.方法:对22例有缺氧缺血性脑损伤新生儿出生后24～48小时内采用电化学发光法检测仪器,抽静脉血测定血清NSE水平.结果:在缺氧缺血性脑病新生儿与非缺氧缺血性脑病患儿进行对照脑损伤后血清NSE 24～48小时内都有明显增高,48～72小时后逐渐降低正常,22例HIE患儿血清NSE明显高于非HIE组,病情越重NSE越高,两者比较差异有显著意义(P＜0.05),HIE组血清CK、LDH比非HIE组明显增高病情越重,增高越明显.NSE越高,血清CK、LDH水平越高,HIE组血清钙和糖水平比非HIE组明显降低,HIE组血清NSE越高低血糖和低血钙发生率比非HIE组明显.结论:血清NSE水平及动态变化与HIE脑损伤密切相关,脑损伤程度越重其增高越明显,故NSE测定可作为HIE患儿判断病情较敏感的指标.     

新生儿缺氧缺血性脑病血清NSE及S-100蛋白的变化及临床意义

目的:探讨缺氧缺血性脑病新生儿血清中NSE、S-100β蛋白水平动态变化及其临床意义.方法:观察组63例(轻度组30例,中度组25例,重度组18例),健康足月新生儿30例为对照组.采用酶联免疫吸附试验双抗体夹心法测定患儿生后24小时、72小时、7天时血清NSE、S-100β蛋白水平及其二者比值,并与对照组进行比较.结果:缺氧缺血性脑病患儿轻度组、中度组和重度组在生后24小时、72小时、7天血清中NSE、S-100β蛋白均明显高于对照组(P＜0.05,0.01),且重度组较轻度组、中度组明显增高(P＜0.05,0.01),其差异具有显著性统计学意义.各组在生后3个时间点的比较,除对照组外,其余各组24小时、72小时均高于7天(P＜0.05),且72小时高于24小时(P＜0.05).结论:动态检测HIE患儿血清中NSE、S-100β蛋白水平,对指导临床诊断和治疗具有一定指导意义,并可通过其变化来推测中枢神经系统并发症的发生,对了解HIE脑损伤程度及判断预后具有重要价值.     

APPLICATION OF LORNOXICAM TO PATIENT-CONTROLLED ANALGESIA IN PATIENTS UNDERGOING ABDOMINAL SURGERIES

FOR anesthesiologis s ,treatingpostoperativepainhas alwaysbeen a problem.Althoughopioidshave been provedtobe effective,theirsideeffectscouldnotbeignored.With thedevelopmentofscienceand pharmacology,many drugs with aspectsof satisfactoryanalgesicefficacyand couldbe welltoleratedby patientshave been developed.And lornoxicamisone of them, which isa non-steroidalanti-inflammatorydrug (NSAID ), with analgesic, anti-infl-ammatory,andantipyreticproperties.Itseliminationhalf-time(3 to 5 hours) isle…     

Dr.Zhang Ren''''s Experience in the Acupuncture Treatment of Different Diseases with the Same Therapeutic Principle

Dr.Zhang Ren,the chief physician,is the chairman of Shanghai Acupuncture and Moxibustion Association.Having been engaged in medicine for about 40 years,he is experienced in treating various intractable diseases.In his long years of clinical practice,he advocates taking the TCM differentiation as the basis to seek for the acupuncture method for treatment of modern intractable diseases.The author of this essay had the fortune to follow Dr.Zhang in study.The following is a summary of Dr.Zhang's experience in the acupuncture treatment for different intractable diseases with the same therapeutic principle.     

Luo Lingjie''''s Experience in Treating Chronic Nephropathy

In treating chronic nephropathy,Luo Lingjie,a chief physician,pays attention to regulating the balance between yin and yang,treating infection if present,and removing pathogenic factors.He prescribes gentle drugs and uses carefully strongly warming-tonifying ones,emphasizes the importance of persuading the patient to persist in treatment with medication and nurse one's health for recuperation,and is good at combined use of TCM and western medicine therapy and brings the merits of various therapies into full play,with obvious theraoeutic effects.     

Acupuncture Combined with Spinal Tui Na for Treatment of Primary Dysmenorrhea in 30 Cases

Objective: To observe the therapeutic effects in acupunture treatment of primary dysmenorrhea combined with spinal Tui Na, and study its mechanism. Methods: Thirty cases of the treatment group were treated by acupuncture combined with spinal Tui Na, and thirty cases in the control group were treated by routine acupuncture. Results: The total effective rate was 93.3% in the treatment group, and 73.3% in the control group, with a significant difference between the two groups (P<0.05). Conclusions: Acupuncture combined with spinal Tui Na has good prospects for treatment of primary dysmenorrhea.     

猪肺磷脂注射液联合经鼻持续气道正压通气对呼吸衰竭早产儿的临床疗效及肌酸激酶同工酶活性的影响

目的 探讨猪肺磷脂注射液联合经鼻持续气道正压通气(NCPAP)对呼吸衰竭早产儿的临床疗效及肌酸激酶同工酶活性(CK-MB)的影响.方法 选取呼吸衰竭早产儿80例,分为观察组和对照组各40例.对照组采用NCPAP给氧治疗,观察组给予NCPAP给氧联合猪肺磷脂气管内给药.观察两组患儿治疗前及治疗12h、24 h后PaO2、PaCO2、血氧饱和度(SaO2)、pH的变化情况,检测治疗前及治疗5d后血清CK-MB水平;评估两组患儿的临床治疗效果.结果 两组患儿PaO2、PaCO2、SaO2、pH比较,差异均有统计学意义(P＜0.05),其中观察组治疗后的PaO2、SaO2、pH均高于对照组,PaCO2则低于对照组.两组的PaO2、SaO2、pH均随观察时间延长而升高(P＜0.05),PaCO2均随观察时间的延长而降低(P＜0.05).观察组治疗有效率为87.5％,显著高于对照组的70.0％ (P ＜0.05).治疗5d后两组患儿血清CK-MB水平均较前降低(P＜0.05),且观察组明显低于对照组(P＜0.05).结论 猪肺磷脂注射液气管内给药联合NCPAP可以显著降低呼吸衰竭早产儿CK-MB的含量,提高治疗有效率,起到很好的呼吸循环支持作用.     

Survey and practice of reporting quality of randomized controlled clinical trials on traditional Chinese medicine

Evidence obtained from randomized controlled trials （RCTs） has been generally accepted as the gold standard in the evaluation of clinical effectiveness. Readers need to understand the trial design, implementation, results, analysis and interpretation, so as to fully Jnderstand the results of RCTs. Thus, the investigators of RCTs have to report these items in a complete, accurate and clear manner. Since 1998, we have conducted several evaluations on the reporting quality of RCTs published in Chinese journals on traditional Chinese medicine （TCM） and results have shown that there is an urgent need for higher quality RCTs on TCM.     

TCM Treatment of Ankylosing Spondylitis by Tonifying the Kidney and Strengthening the Governor Vessel

Ankylosing spondylitis is a chronic and progressive disorder with inflammation mainly involving the central axis joints. It mainly affects the cervical spine and the lumbosacral area, with the pathogenesis closely related to the kidney and the Governor Vessel (GV). TCM holds that the syndrome is deficiency in origin and excess in superficiality, which is due to insufficiency of the kidney, deficiency of GV, and blocking of the channels with the invasion of exogenous evil, leading to poor circulation of qi and blood and malnutrition of the bones, muscles and joints. The TCM method of tonifying the kidney and strengthening GV to regulate circulation of qi and blood and check the arthralgia pain should be adopted, with the Kidney-Tonifying and GV Strengthening Decoction (益肾强督汤) prescribed.     

IMPROVING P-gp EXPRESSION IN HUMAN MONONUCLEAR CELLS IN VITRO TRANSFECTED BY MULTIDRUG RESISTANCE-1 mRNA

CHEMOTHERAPY playsa greatrolein the treat- ment of malignanttumors,especiallyingynecolo- gicalones.But inanticancerchemotherapy,leuko-cytopeniaisfrequentlytheprimarydose-limitingsideeffect factor.Moreover,cancersarefrequentlychemoresistantbe-causeof overexpressionof P-glycoprotein(P-gp), which isencodedby multidrugresistancegene (MDR1 ) and detectableinup to50% ofhuman cancersand renderscellsresistancetoanticancerdrugs.The safetyand potentialtherapeuticbenefitof mdr1 gene transferredto h…