分光光度法测定药物制剂中的锌

基于锌与硫氰酸钾、结晶紫在酸性介质中,有阿拉伯树胶存在条件下生成兰色缔合物的反应,以分光光度法测定药物制剂中锌的含量。其吸收波长λmax=545nm,Beer定律范围为1～5μg/10ml,摩尔吸光系数∑545=1.42×10~5L·mol~(-1)·cm~(-1),回收率为100.3±0.32％,且制剂中辅料无干扰。本法操作简便,灵敏度高,测定结果满意。     

用硫酸钴催化反式-二水·二草酸根络铬(Ⅲ)的顺反异构化反应

用硫酸钴催化反式-二水·二草酸根络铬(Ⅲ)的顺反异构化反应,当离子强度为1.5mol·L~(-1)、pH为3及温度分别为25、35和45℃时,发现Co~(2+)浓度不大时和反应速度成直线关系。Co~(2+)浓度大于0.15mol·L~(-1)时,25℃的反应速度大于直线关系。表明Co~(2+)和CoSO_4离子对,同时具有催化作用。测得25℃时k_(Co~(2+))=2.13×10~(-2)(mol·L~(-1)·s)~(-1),k_(CoSO_4)=5.77×10~(-3)(mol·L~(-1)·s)~(-1),并测得Co~(2+)和COSO_4的活化参数分别为 △S?=-26.4和-96.2J·K~(-1)·mol~(-1),△H?=74.9和 56.9kJ·mol~(-1)。△E?_(298)=77.4和59.4kJ·mol~(-1)。根据活化参数推论该反应为开环机理。     

对苯醌与磺胺二甲嘧啶荷移反应研究

本文研究了对苯醌与磺胺二甲嘧啶荷移反应的条件、络合物的组成,初步探讨了对苯醌与磺胺二甲嘧啶反应的动力学性质。在醇酸性介质中,对苯醌与磺胺二甲嘧啶形成1:1的橙红色络合物,其ε=4.9×10~3l·mol~(-1),λ=490nm。     

对-磺基苯重氮氨基偶氮苯—双波长胶束增溶分光光度法测定水中痕量镉

本文报道用双波长胶束增溶分光光度法测定水中痕量镉。方法基于在非离子型表面活性剂TritonX-100存在下镉与对-磺基苯重氮氨基偶氮苯(SDAAB)的显色反应。研究表明,于pH10.80的缓冲介质中及在Triton X-100的存在下,镉与SDAAB形成红色络合物,其最大吸收波长位于525nm处。络合物非常稳定,在10天内吸光度保持不变,其mol吸光系数高达2.84×10~5L·mol~(-1)·cm~(-1)。镉浓度在0～4μg/25ml范围内符合比耳定律。用平衡移动法和Asmus直线法测得镉与SDAAB的络合比均为1:2。经实践证明,由于用此法测定水中的镉具有简便、准确、灵敏度极高,选择性和重现性好等优点,故有较大的实用价值。     

海军陆战队登陆训练热能消耗

用“气体代谢法”测定海军陆战队登滩冲击动作热能消耗率.结果为0.0451±0.0018MJ/m~2·min~(-1)(10.7763±0.4402kcal/m~2·min~(-1))。用“生活观察法”测得登陆场训练时24h 热能消耗量为17.17±1.39MJ(4103.0±332.7kcal),属极重劳动。     

利用头孢霉素钯配合物分光光度测定某些一、二代头孢霉素

头孢噻吩、头孢噻啶、头孢呋新和头孢孟多,能与pd~(2+)生成稳定的配合物.头孢霉素Pd~(2+)配合物皆有色,在400nm可进行可见分光光度测定.此法简单、快速、重复性好,ε值为2800～5600 l·mol~(-1)·cm~(-1).也可用偏苯三酚酞—CTAB为试剂的胶束增溶分光光度法,测定头孢霉素与过量pd~(2+)的反应液中未结合的游离钯,间接测定微量的头孢霉素.此法灵敏,有效摩尔“褪色”系数大于1×10~5 l·mol~(-1)·cm~(-1)     

维生素C分光光度分析法的研究——Ⅰ.用新显色剂2,5-二硝基苯酚作分光光度测定片剂中维生素C的含量

本文报道2,5-二硝基苯酚在0.5mol/L氢氧化钠介质中及在40℃恒温水浴中加热15min的条件下与维生素C作用生成红色还原产物,其最大吸收波长位于500nm。维生素C浓度介于0.03～0.1mg/ml之间服从比耳定律,其mol吸光系数为3.2×10~3L·mol~(-1)·cm~(-1),Sandell灵敏度为0.055μg/cm~2。红色还原产物的色泽可稳定24h。本法曾用于药物片剂中维生素C含量的测定获得满意结果。     

普卢利沙星的吸附伏安特性及其应用

目的探讨普卢利沙星的吸附伏安特性。 方法在03?mol·L-1KH2PO4 Na2HPO4 (pH640)底液中,普卢利沙星(prulifloxacin,PLF)在汞电极上有一线性扫描还原峰,峰电位EP=-102?V(vsAg／AgCl),该峰具有明显的吸附性。 结果吸附粒子为PLF中性分子,测得PLF在汞电极上的饱和吸附量Γs=401×10-11mol·cm-2,每个PLF分子所占电极面积为202?nm2,PLF在汞电极上的吸附符合Langmuir吸附等温式;测得吸附系数β=108×106,25?℃时的吸附自由能ΔGθ=-2914?kJ·mol-1,电极反应电子数n=2,不可逆体系动力学参量αna=101,表面电极反应速率常量ks=027?s-1。结论建立了吸附溶出伏安法测定PLF的最佳条件,方法的检出限为20×10-8mol·L-1。     

亚硝酸根与中性红显色反应的分光光度研究及其分析应用

本文研究了亚硝酸根与中性红显色反应的条件。在0.09～0.4mol/L HCl介质中,亚硝酸根与中性红生成紫蓝色化合物,其最大吸收峰位于535nm处,反应灵敏(∑_(645)nm=7.40×10~3和9.81×10~3L·mol~(-1)·cm~(-1),选择性好。亚硝酸根含量在0～5μg/25ml及5～25μg/25ml范围内遵守比尔定律。本法曾用于水、土壤及蔬菜中微量亚硝酸根的测定获得满意的结果。     

家兔静注甲硝唑磷酸酯钾及甲硝唑药代动力学研究

作者以紫外法对家兔静注甲硝唑磷酸酯钾(PMP)及甲硝唑(MTZ)进行了体内药代动力学初步研究和比较。静注PMP及MTZ后其药代动力学特征符合二室开放模型。PMP药动学参数为:T_(1/2)x:0.019±0.007·h~(-1);T_(1/2)β:1.12±0.62·h~(-1);K_(1.2):26.86±9.7·h~(-1);K_(2.1):9.53±4.66·h~(-1);K_:4.04±2.81L·kg~(-1);V_B:0.52±0.11L·kg~(-1);CLB:0.47±0.3L·kg~(-1)·h;AUC:96.32±46.16mg·L·h~(-1)。MTZ药动学参数为:T_(1/2)x:0.043±0.029·h~(-1);T_(1.2)~(-1)]β:1.00±0.47·h~(-1);K_(1.2):9.91±5.60·h~(-1);K_(2.1):19.53±13.39·h~(-1);K_(el):2.14±2.25L·kg~(-1);V_B:0.58±0.23L·kg~(-1);CLB:0.56±0.55L·kg~(-1)·h~(-1);AUC:47.56±22.14mg·L~(-1)·h~(-1)。结果表明静注PMP及MTZ其药动学特征相似。     

APPLICATION OF LORNOXICAM TO PATIENT-CONTROLLED ANALGESIA IN PATIENTS UNDERGOING ABDOMINAL SURGERIES

FOR anesthesiologis s ,treatingpostoperativepainhas alwaysbeen a problem.Althoughopioidshave been provedtobe effective,theirsideeffectscouldnotbeignored.With thedevelopmentofscienceand pharmacology,many drugs with aspectsof satisfactoryanalgesicefficacyand couldbe welltoleratedby patientshave been developed.And lornoxicamisone of them, which isa non-steroidalanti-inflammatorydrug (NSAID ), with analgesic, anti-infl-ammatory,andantipyreticproperties.Itseliminationhalf-time(3 to 5 hours) isle…     

Acupuncture Treatment of Vomiting

CASE HISTORY A female patient, 46 years old, head of the foreign affairs department of a certain university in Beijing, paid her first visit on October 9, 2006, with the chief complaint of vomiting for one month. She got vomiting after meals in early September. Before that, she had discomfortable sensation in the stomach due to angry with others, but she didn't pay much attention. Later, it developed into vomiting after eating. After the vomiting, the discomfort would be relieved, but with slight hypodynamia. She was once diagnosed as having 'neurogenic vomiting'. Having taken some western and Chinese drugs, the above symptoms were a little bit improved, but she would have nausea upon eating and with regurgitation. Because of the fear for vomiting, she did not dare to have food intake, with body weight reduction of 6 kilos in one month.     

Qiu Baoguo''''s Experience in Treating Consumptive Syndromes after Radio-Chemotherapies

Radiotherapy and chemotherapy are the important modern medical therapies for malignant tumors,yet they can also bring about serious local and systemic toxic side reactions so to decrease the patient;'s life quality,manifested by a series of consumptive symptoms.Having engaged in the combined work of Chinese and western medicine for nearly 50 years,the research fellow Qiu Baoguo in Henan Provincial Academy of TCM has developed his unique views on the TCM study of consumptive syndromes.The author of this essay had once the fortune tO follow Dr.Qiu in clinic,and specially would like to introduce in the following Dr.Qiu's experience in treating consumptive syndromes after radio-chemotherapies for patients with malignant tumor.     

Insomnia Due to Deficiency of Both the Heart and Spleen Treated by Acupuncture-Moxibustion and Chinese Tuina

OBJECTIVE: To observe therapeutic effects of the comprehensive therapy of acupuncture-moxibustion and Chinese Tuina for treatment of insomnia due to deficiency of both the heart and spleen. METHODS: 92 cases were divided randomly into the treatment group (treated by acupuncture-moxibustion and Chinese Tuina) and the control group (treated by acupuncture-moxibustion). RESULTS: The therapeutic effect of the treatment group was obviously superior to that of the control group (the CHI2 test showed P < 0.01). CONCLUSIONS: The comprehensive therapy of acupuncture-moxibustion and Chinese Tuina can give marked therapeutic effects for treatment of insomnia due to deficiency of both the heart and spleen.     

Analysis of CK-19,PDX-1,Nestin,Ngn3 in different donor islet purity in rats

Objective: To investigate if there are the CK-19, PDX-1, Nestin, Ngn3 positive cells in the donor islets of different purity in rats. Methods: Thirty male adult SD rats were randomly divided into 3 groups. Islets were isolated using digestion by ductal injection of collagenase. Group Ⅰ (n=10): Separating cell preparations were not purified, Group Ⅱ(n=10): Islet sediment was purified with 25% Ficoll400 ,Group Ⅲ (n=10): Islet sediment was purified with 25% and 11% Ficoll-400. The levels of protein of CK-19, PDX-1, Nestin and Ngn3 were detected by immunohistochemistry and the mRNA of CK-19, PDX-1, Nestin, Ngn3 was amplified by RT-PCR. Results: After two different purification methods applied, three islet preparations of different purities were obtained. The difference of islet purity was significant among various groups (P＜0.05). Compared with group Ⅱ and group Ⅲ,the protein and mRNA of CK-19, PDX-1, Nestin,Ngn3 were both higher in group Ⅰ; group Ⅲ was poorly expressed. Conclusions: The three different islet purity donor islet have different CK-19, PDX-1, Nestin, Ngn3 positive cells within them, indicating that there are some islet stem cells in the purified donor islet.     

Dr.Zhang Ren''''s Experience in the Acupuncture Treatment of Different Diseases with the Same Therapeutic Principle

Dr.Zhang Ren,the chief physician,is the chairman of Shanghai Acupuncture and Moxibustion Association.Having been engaged in medicine for about 40 years,he is experienced in treating various intractable diseases.In his long years of clinical practice,he advocates taking the TCM differentiation as the basis to seek for the acupuncture method for treatment of modern intractable diseases.The author of this essay had the fortune to follow Dr.Zhang in study.The following is a summary of Dr.Zhang's experience in the acupuncture treatment for different intractable diseases with the same therapeutic principle.     

Luo Lingjie''''s Experience in Treating Chronic Nephropathy

In treating chronic nephropathy,Luo Lingjie,a chief physician,pays attention to regulating the balance between yin and yang,treating infection if present,and removing pathogenic factors.He prescribes gentle drugs and uses carefully strongly warming-tonifying ones,emphasizes the importance of persuading the patient to persist in treatment with medication and nurse one's health for recuperation,and is good at combined use of TCM and western medicine therapy and brings the merits of various therapies into full play,with obvious theraoeutic effects.     

Acupuncture Combined with Spinal Tui Na for Treatment of Primary Dysmenorrhea in 30 Cases

Objective: To observe the therapeutic effects in acupunture treatment of primary dysmenorrhea combined with spinal Tui Na, and study its mechanism. Methods: Thirty cases of the treatment group were treated by acupuncture combined with spinal Tui Na, and thirty cases in the control group were treated by routine acupuncture. Results: The total effective rate was 93.3% in the treatment group, and 73.3% in the control group, with a significant difference between the two groups (P<0.05). Conclusions: Acupuncture combined with spinal Tui Na has good prospects for treatment of primary dysmenorrhea.