中华猕猴桃果实化学成分的研究

目的对中华猕猴桃Actinidia Chinensis Planch.果实的化学成分进行研究。方法果实的乙酸乙酯提取物采用硅胶、Sephadex LH-20凝胶、反相ODS柱等方法进行分离纯化,根据所得化合物的波谱数据确定其结构。结果从中分离得到8个化合物,分别鉴定为3β-乙酰氧基-12-烯-28-乌苏酸(1)、2α,3β-二羟基-12-烯-28-乌苏酸(2)、2α,3α-二羟基-12-烯-28-乌苏酸(3)、2α,3α-二羟基-12-烯-28-齐墩果酸(4)、齐墩果酸(5)、乌苏酸(6)、胡萝卜苷(7)、β-谷甾醇(8)。结论化合物1~4为首次从中华猕猴桃果实中分离得到。     

美味猕猴桃根活性化学成分的研究

从美味猕猴桃根中分离有活性的化合物。采用溶剂提取法和多种柱层析的方法进行分离,利用理化分析和波谱技术对化合物进行鉴定。共分离出5个化合物,分别鉴定为2,α3,β19,23-四羟基-12-烯-28-齐墩果酸(Ⅰ),2,α3,α23-三羟基-12-烯-28-乌苏酸(Ⅱ),2,α3,β19,23-四羟基-12-烯-28-乌苏酸(Ⅲ),正十一酸(Ⅳ),beta-si-tosterol(Ⅴ)。Ⅰ、Ⅳ、Ⅴ为首次从猕猴桃根中得到。     

枸骨根的化学成分研究

目的研究冬青科冬青属植物枸骨Ilex cornuta根中的化学成分。方法运用硅胶、Sephadex LH-20柱色谱对枸骨根中的化学成分进行分离,通过理化常数、质谱和核磁技术鉴定化合物。结果从枸骨根中分离并鉴定了11个化合物,分别为β-谷甾醇(1)、羽扇豆醇(2)、白桦酸(3)、常春藤皂苷元(4)、3β-乙酰基-28-羟基-乌苏醇(5)、乌苏酸(6)、19α-羟基乌苏酸(7)、3β-乙酰基-乌苏酸(8)、23-羟基-乌苏甲酯(9)、庚酸(10)、β-胡萝卜苷(11)。结论化合物4、5、8、9为首次从该属植物中分离得到。化合物3为首次从该植物中分离得到。     

蓝桉果实一口钟化学成分研究

目的研究一口钟(蓝桉Eucalyptus globulus果实)中的化学成分。方法综合运用正相硅胶柱色谱、凝胶渗透柱色谱、半制备HPLC色谱等方法分离、纯化一口钟中的化学成分,采用NMR和质谱等波谱学方法鉴定化合物结构。结果从一口钟中分离得到12个化合物,分别鉴定为1,10-dioxotayloriane(1)、litseagermacrane(2)、蓝桉醇(3)、表蓝桉醇(4)、绿花白千层醇(5)、齐墩果酸(6)、白桦脂酸(7)、熊果酸(8)、11α-甲氧基熊果酸(9)、1-(2,6-dihydroxy-4-methoxy-3,5-dimethylphenyl)-2-methylbutan-1-one(10)、鞣花酸(11)、3-甲氧基鞣花酸-4′-O-α-L-吡喃鼠李糖苷(12)。结论化合物1和2为首次从该属植物中分离得到,化合物9和10为首次从该种植物中分离得到。     

美味猕猴桃根中三萜类成分研究

目的 研究美味猕猴桃Actinidia deliciosa根的化学成分.方法 以95％乙醇为提取溶剂,经多种色谱方法分离,根据理化性质,结合谱学手段对化合物进行结构鉴定.结果 从美味猕猴桃根中分离得到3个化合物,分别鉴定为2β,3β,28-三羟基-12,20(30)-羽扇豆二烯(1)、2α,3β,19α,24-四羟基-12-烯-28-乌苏酸(2)、2α,3β,23,27-四羟基-12-烯-28-乌苏酸(3).结论 化合物1为1个新的羽扇豆烷型三萜,命名为猕猴桃素A.     

中华猕猴桃根的化学成分研究

目的：研究中华猕猴桃Actinidia chinensis根的化学成分。方法：中华猕猴桃根的95％乙醇提取物,依次用石油醚、醋酸乙酯、正丁醇萃取,应用多种色谱技术对醋酸乙酯部分进行分离纯化,通过波谱数据分析(MS,IR,¹H-NMR,¹³C-NMR)进行结构鉴定。结果：从醋酸乙酯部分分离得到7个化合物,经鉴定为2α-羟基齐墩果酸(1),2α-羟基乌苏酸(2),蔷薇酸(3),23-羟基乌苏酸(4),3β-O-乙酰乌苏果酸(5),麦角甾-4,6,8(14),22-四烯-3-酮(6),β-谷甾醇(7)。结论：所有化合物均为首次从该植物中分离鉴定。     

石崖茶的化学成分研究

目的研究石崖茶Adinandra nitida的化学成分。方法运用色谱方法分离纯化,经波谱数据和理化性质鉴定化合物结构。结果从石崖茶的甲醇提取物中分离鉴定13个萜类化合物,包括11个三萜:乌索酸(1)、18-羟基乌索酸(2)、2α,3α-二羟基乌苏酸(3)、3α,19α-二羟基乌苏酸(4)、蔷薇酸(5)、3β,19α,23-三羟基酸(6)、2α,3α-二羟基乌苏酸-28-O-β-D-葡萄糖苷(7)、kajiichigaside F1(8)、齐墩果酸(9)、arjunetin(10)、白桦脂酸(11),2个二萜:cassipourol(12)、α-生育酚(13)及2个甾体化合物:胡萝卜苷(14)、β-谷甾醇(15)。结论化合物1~4、6、7、9、11~13为首次从石崖茶中分离得到。     

金樱子根三萜类的化学成分研究

目的研究金樱子Rosa laevigata根中的化学成分。方法利用各种色谱柱对金樱子根中的化学成分进行分离纯化,并根据理化性质和光谱分析进行结构鉴定。结果从金樱子根的二氯甲烷及醋酸乙酯部位中分离得到13个三萜类成分,分别鉴定为niga-ichigosides F2(1)、野蔷薇亭(2)、阿江榄仁亭(3)、构莓苷F1(4)、蔷薇酸(5)、号角树酸3-甲酯(6)、2-乙酰基-洋委陵菜酸(7)、坡模酸(8)、2α,3α-二羟基乌苏-12,18-二烯-28-酸(9)、3β-E-feruloyl corosolic acid(10)、覆盆子酸(11)、2-O-乙酰基野鸭椿酸(12)、12,13-dihydromicromeric acid(13)。结论化合物3、6、7、9~13为首次从该植物中分离得到;化合物10、12、13为首次从蔷薇属中分离得到。     

龙船花化学成分研究

目的:研究龙船花的化学成分。方法:采用硅胶、Sephadex LH-20、MPLC等色谱方法对龙船花化学成分进行分离纯化,根据化合物的理化性质、波谱分析方法结合文献报道对分离得到的化合物进行结构鉴定。结果:从龙船花石油醚提取部位、乙酸乙酯提取部位分离纯化得到10个化合物,分别鉴定为β-谷甾醇(1)、2α,3α-二羟基-12-烯-28-乌苏酸(2)、3-O-乙酰坡模酸(3)、对羟基桂皮酸(4)、绿原酸(5)、儿茶素(6)、(-)-表儿茶素(7)、异鼠李素(8)、京尼平苷(9)、胡萝卜苷(10)。结论:化合物2～8为首次从该植物中分离得到。     

独子藤根皮醋酸乙酯部位的化学成分研究

目的研究独子藤Celastrusmonospermus根皮醋酸乙酯部位的化学成分。方法采用硅胶、反相硅胶和凝胶等柱色谱方法进行分离纯化,通过波谱学数据鉴定化合物的结构。结果从独子藤根皮醋酸乙酯部位中分离得到12个化合物,分别鉴定为美登木酸(1)、对羟基苯甲酸(2)、4-羟基-3-甲氧基苯甲酸(3)、大子五层龙酸(4)、3-氧代-2β-羟基木栓烷-29-酸(5)、雷公藤红素(6)、3α,22β-二羟基齐墩果烷-12-烯-29-酸(7)、直楔草酸(8)、21-氧代-2α,3α,22β-三羟基-29-降-木栓烷-24,2β-内酯(9)、2,3-二氧代-6α,10-二羟基-24-降-木栓烷-4,7-二烯-29-酸(10)、2α,3β,19α,23-四羟基-齐墩果烷-12-烯-28-酸(11)、2α,3β-二羟基-齐墩果烷-12-烯-23,28,30-三酸(12)。结论化合物1、3、7、10～12为首次从本植物中分离得到,3、11、12为首次从本属植物中分离得到。     

微波消解-电感耦合等离子体质谱法测定脉络宁注射液中25种矿物质元素

目的建立微波消解-电感耦合等离子体质谱(ICP-MS)直接稀释测定脉络宁注射液中25种矿物质元素(Mg、Ca、Fe、Cu、Zn、Mn、Al、B、Ba、Co、Cr、K、Li、Mo、Na、Ni、P、Pb、Sr、Th、Ti、V、As、Cd和Hg)的方法。方法分别对微波消解条件和测试条件进行考察;样品经微波消解后,采用电感耦合质谱仪测定25种矿物质元素,并对测定方法学进行考察。结果确定最佳消解条件为3步缓慢升温:400 W 80℃升温10 min,保留5 min;600 W 120℃升温10 min,保留5 min;900 W 200℃升温20 min,保留20 min;25种矿物质元素在各自的线性范围内线性关系良好,r≥0.999 6,精密度、稳定性和重复性试验的RSD均符合定量分析要求;加标回收率为94.7%~106.1%,RSD在0.34%~2.79%。脉络宁注射液中检测出Mg、Ca、Fe、Cu、Zn、Mn、Al、B、Ba、Co、Cr、K、Li、Mo、Na、Ni、P、Pb、Sr、Th、Ti、V,未检出As、Cd和Hg。结论该方法简便、迅速、准确,适用于脉络宁注射液中25种矿物质元素的同时测定。     

Die Lateralität und Lateralitätsinstabilität in der Diagnostik und Therapie

The disturbance and instability of laterality are obstacles to diagnostics and therapy. Correction prior to starting therapy is required. They also predispose toward defined health problems and unspecific diseases. Numerous research activities provide evidence of the relevance of undisturbed laterality in diagnostics and therapy. Techniques of testing and therapeutic corrections will allow for optimized therapy success.     

Yerba Mate or Paraguay Tea

正Beverages based on Ilex paraguariensis A.St.-Hil.are used in the south Brazil and other Latin American countries located at the so-called southern cone.It is known as chimarro or mate in south and southeast Brazil,tererêin     

Journal of Acupuncture and Tuina Science Instructions for Authors

The Journal of Acupuncture and Tuina Science, a journal with an international scope （IS SN 1672-3597, CN 31-1908/R, Bimonthly）, is embodied by ＇Springer Verlag＇ Database, Index Copernicus （IC） and Chinese Scientific and Technical Paper and Citations Data （CSTPCD）. You can search full text on http：//www, springerlink, com/content/1672 -3597.     

Ethnopharmacologic study of chinese rhubarb

This paper deals with an interdisciplinary study covering historic, botanical, phytochemical, pharmacological and clinical aspects of rhubarb and related species, to lay stress on the correlation between plant phylogeny, chemical constituents and purgative activity.

It was found that the official rhubarbs were exclusively restricted in the Sect. Palmata e.g. Rheum palmatum R. palmatum var. tanguticum R. officinale; the following criteria may serve as their standard, viz., the presence of sennoside derivatives and rhein, the occurrence of the reduced form of rhein and aloe-emodin, the leaves with any kind of palmate incision. Comprehensive multivariate analyses showed that there is a very close relationship between the leaf incision, existence of sennosides or rhein and purgative activity.     

基于ICP-MS法的加味左金丸中无机元素分析

目的 通过电感耦合等离子体质谱（inductively coupled plasma mass spectrometry,ICP-MS）法建立加味左金丸中Cd、Pb、As、Hg、Co、V、Ni、Cu、Li、Sb、Ba、Mo、Sn、Cr、Na、Mg、Al、Ca、Ti、Mn、Fe、Zn、Ga、Se、Sr、Tl共计26种无机元素的测定方法。方法 加味左金丸通过微波消解法处理后,根据相对分子质量的大小选择内标物,其中⁷Li、²³Na、²⁴Mg、²⁷Al、⁴⁰Ca、⁴⁸Ti、⁵¹V、⁵²Cr、⁵⁵Mn、⁵⁶Fe、⁵⁸Ni、⁵⁹Co、⁶³Cu、⁶⁶Zn、⁷⁰Ga、⁷⁵As、⁷⁷Se、⁸⁶Sr以⁷²Ge作为内标;⁹⁵Mo、¹¹⁴Cd、¹¹⁸Sn、¹²¹Sb、¹³⁷Ba以¹¹⁵In作为内标;²⁰²Hg、²⁰⁵Tl、²⁰⁸Pb以²⁰⁹Bi作为内标。对标准品溶液、空白溶液与供试品溶液进行分析,采用标准曲线法进行定量分析。通过ICP-MS法进行测定。结果 26种无机元素线性的相关系数r ≥ 0.999 6,检出限为0.001~1.500μg/L,定量限为0.01~5.00 μg/L,精密度与重复性试验的RSD均小于5%,平均回收率在82.64%~106.44%,RSD均小于5%。对3个厂家的12批样品进行了测定,26种元素的含量差异较大,其中Na、Mg、Ca、Fe 4种元素的含量比较高,均大于500 μg/g,Cd、Pb、As、Hg、Co、Li、Sb、Mo、Sn、Cr、Se、Tl的含量比较低,均小于1 μg/g。由结果可知,人体的常量元素,如Na、Mg、Ca的含量比较高,Cd、Pb、As、Hg等有害元素含量比较低。根据《中国药典》2020年版一部的要求,本品中Cd、Pb、As、Hg与Cu均符合规定。结论 该方法快速、准确,可以用于加味左金丸中无机元素的测定。     

Wirksamkeit der Akupunktur bei der Therapie von idiopathischen Schmeckstörungen: Eine randomisierte Placebo-kontrollierte Studie

Background

At present, the state-of-the-art medication in treating idiopathic taste disorder (gustatory dysfunction) is zinc. If zinc medication was unsuccessful, not tolerated or if it is contraindicated the persons affected can hardly be helped.

Objectives

In a randomized placebo-controlled clinical trial an attempt was made to determine the efficacy of acupuncture.

Methods

The treatment group was treated with acupuncture needles, the placebo group with an inactive acupuncture-laser. Endpoints of the study were changes in the taste test, the quality of life and the severity of symptoms of depression (BDI) as well as mood changes (ZMS). Satisfaction with the success of the respective therapy was also evaluated. Data were collected before and after therapy and again for the treatment group 10 weeks after completion of the treatment period.

Findings

Treatment with real acupuncture showed a significant improvement in gustatory function as well as a significant improvement in psychological symptoms. Greater satisfaction with the treatment results was evident in the treatment group. The treatment results of acupuncture remained stable over a period of ten weeks after completion of the treatment.

Conclusion

Acupuncture was effective in the therapy of idiopathic taste disorders and therefore it is an effective alternative when zinc medication is not tolerated or unsuccessful. But it is also suitable as a therapy of first choice. Acupuncture can be considered as an extension of the therapeutic spectrum in the difficult treatment of taste disorders.     

Erstaunliches Ergebnis einer Schädelakupunktur nach Yamamoto bei Hemihypsarrhythmie rechts

A girl, three and a half years old at the respective date, premature in 25th week of gestation, had been presented. On day three after birth, she had had a IVH III° right and left with severe involvement of parenchyma and other multiple complications, e. a. infant respiratory distress syndrome up to the 42nd day postpartum, and epileptic attacks with apnoeic events.     

Veränderungen der Herzratenvariabilität (HRV) während Low Level Lasernadelakupunktur: Placebokontrollierte Studie an 19 herzgesunden Probanden

Background

Experimental and clinical studies postulate a neurobiological mechanism of acupuncture by modulating central pain systems, neurohormones and -transmitters. It seems to be quite likely that the autonomic nervous system (ANS) plays an important mediating role in this neuromodulation.

Aim

In measuring heart rate variability (HRV), as a non-invasive approach to the ANS-function, the object of this study was to monitor probable acupuncture-induced changes of autonomic balance. The innovative technology of laser-needle acupuncture offers for the first time a stimulation method which fulfils the demands on a placebo-controlled acupuncture trial not requiring controversial sham acupuncture.

Method

19 healthy voluntary subjects underwent 3 treatments in randomized sequence, consisting of placebo-, 1-point- (PC 6) and simultaneous 3-point- (PC 6, LR 3, SI 3) laser-needle-stimulation over 20 minutes. Before, during and after placebo-/verum laser-needle acupuncture, HRV-data for normalized HF(high frequency)- and LF(low frequency)-Power (nHF, nLF) and their quotient nLF/nHF obtained by power spectrum analysis (FFT), were measured and analyzed statistically at 5 different time points (ANOVA repeated measures, p < 0,05).

Results

During both verum applications, the 1-point- (PC 6) as well as the simultaneous 3-point- (PC 6, LR 3, SI 3) laser-needle stimulation, significant changes of the ANS-activity were found in comparison to the placebo application. The most significant modification of HRV was an increase of nHF (p < 0,05 (PC 6), p < 0,0001 (PC 6, LR 3, SI 3)) as an expression of growing vagal activity during acupuncture.

Conclusion

Laser-needle acupuncture causes in 1-point-stimulation (PC 6) as well as in simultaneous 3-point-stimulation (PC 6, LR 3, SI 3) significant changes in HRV in comparison to placebo application. HRV-monitoring seems to be a suitable approach in exploration of acupuncture-induced changes of ANS and could possibly be established in combination with laser needle acupuncture as a standard for further scientific and clinical acupuncture investigations which are greatly needed.