柯萨奇B_4和B_(3M)病毒重叠感染对低硒昆明鼠T细胞亚群的影响

目的：观察低硒,补硒鼠先后感染ＣＶＢ４,ＣＶＢ３Ｍ两种病毒七天后,小鼠脾脏Ｔ细胞亚群的变化。方法：小鼠先后感染ＣＶＢ４,ＣＶＢ３Ｍ两种病毒七天后,检测脾脏Ｔ细胞亚群细胞数,血清中和抗体及心肌组织病毒滴度。结果：在低硒感染组ＣＤ４,ＣＤ８,ＣＤ３均显著下降,而补硒感染组ＣＤ３,ＣＤ４,ＣＤ８Ｔ淋巴细胞亚群都明显升高,心肌组织病毒,病变程序及检出率低硒病毒组显著重于补硒组。血清中和抗体效价低硒组显著低于补硒组。结论：硒缺乏可以导致机体免疫功能的低下,而ＣＶＢ４,ＣＶＢ３Ｍ病毒的二重感染导致低硒鼠的免疫功能进一步低下。     

低硒与Coxsackie B3m病毒感染对BALB／c小鼠肝脏脂质过氧化物？…

用国产低硒食用酵母合成低硒饲料（硒０．０１３ｍｇ／ｋｇ，ＶＥ含量为２０ｍ／１００ｇ）喂养断奶后ＢＡＬＢ／Ｃ雄性小鼠，５周后腹腔接种嗜鼠心肌病毒ＣＶＢ３ｍ１０＾３ＴＣＤ５００．１ｍｌ７天后处死建立低硒状态下病毒性心肌炎模型，测定肝脏组织中脂质过氧化物（ＬＰＯ）含量及全血中谷胱甘肽过氧化物酶（ＧＳＨ－Ｐｘ）活性。结果表明低硒感染ＣＶＢ３ｍ小鼠肝组织中ＬＰＯ含量明显高于常硒病毒感染组及常硒对照组（Ｐ〈０     

柯萨奇B3m病毒致低硒BALB/C成年鼠心肌损伤特点实验研究

应用低硒和补硒合成饲料喂养５周龄ＢＡＬＢ／Ｃ雄性小鼠５周后，经腹腔接种柯萨奇Ｂ３ｍ病毒（ＣＶＢ３ｍ）１０＾３ＴＣＩＯＤ５００．１ｍｌ对照组腹腔注射ＰＲＭ１６４０，光镜下低硒病毒组（１组），补硒病毒组（２组）病变检出率分别为７５％和３５％，经Ｘ＾２检验１组显高于２组（Ｐ〈０．０５）。且病变部位不同，低硒病毒１组病变主要位于左心室中层，大体标本未见心肌外膜白斑。２组见于心外膜及心外膜下心肌，大体标本     

低硒与Coxsackie B_(3m)病毒感染对BALB/c小鼠肝脏脂质过氧化物代谢的影响

用国产低硒食用酵母合成低硒饲料（硒０．０１３ｍｇ／ｋｇ，ＶＥ含量为２０ｍｇ／１００ｇ）喂养断奶后ＢＡＬＢ／Ｃ雄性小鼠，５周后腹腔接种嗜鼠心肌病毒ＣＶＢ３ｍ１０３ＴＣＤ５００．１ｍｌ，７天后处死建立低硒状态下病毒性心肌炎模型，测定肝脏组织中脂质过氧化物（ＬＰＯ）含量及全血中谷胱甘肽过氧化物酶（ＧＳＨ－Ｐｘ）活性。结果表明低硒感染ＣＶＢ３ｍ小鼠肝组织中ＬＰＯ含量明显高于常硒病毒感染组及常硒对照组（Ｐ＜０．０１）；低硒感染ＣＶＢ３ｍ病毒组小鼠全血ＧＳＨ－Ｐｘ活力也最低。结果提示：低硒因素加重病毒感染引起ＧＳＨ－Ｐｘ活力降低，ＬＰＯ堆积，降低机体的抗氧化能力     

阿柯萨B3m病毒致低硒鼠心肌病变检出率与病毒中和抗体？…

５周龄ＢＡＬＢ／Ｃ雄性鼠经低硒,常合成饲料喂养５周后,腹腔注射ＣｏｓａｃｋｉｅＢ３ｍ病毒（ＣＶＢ３ｍ）１０００ＴＣＤ５００．１ｍｌ,对照组注射１６４０营养液０．１ｍｌ,７天后处列主脏常规切片,ＨＥ染色光镜下检查心肌病变。取血清测定ＣＶＢ３ｍ病毒中和抗体效价。     

病毒性心肌炎可溶性白细胞介素2受体、自然杀伤细胞活性及T细胞亚群的检测

应用单克隆与多克隆双抗体夹心法检测３６例病毒性心肌炎（ＶＭＣ）及２４例正常人（ＮＣ）的血清可溶性白细胞介素２受体（ｓＩＬ－２Ｒ），同时测定外周血自然杀伤细胞（ＮＫＣ）活性和Ｔ淋巴细胞亚群。结果显示ＶＭＣ患者ｓＩＬ－２Ｒ明显高于ＮＣ组（Ｐ＜０．００１）．而ＮＫＣ活性明显低于ＮＣ组（Ｐ＜０．０１），Ｔ细胞亚群与ＮＣ组比较，急性ＶＭＣ患者总Ｔ细胞（ＣＤ３），辅助性Ｔ细胞（ＣＤ４）和抑制性Ｔ细胞（ＣＤ８）均减少，ＣＤ４／ＣＤ８比值显著降低（Ｐ＜０．０５．０．０１），以细胞免疫功能低下为明显；而ＶＭＣ后遗症期患者ＣＤ３、ＣＤ４与ＮＣ组无差异（Ｐ＞０．０５），ＣＤ８显著降低（Ｐ＜０．０５），ＣＤ４／ＣＤ８比值显著高于ＮＣ组（Ｐ＜０．０５），以细胞免疫调节失衡为主。上述结果提示细胞免疫功能低下及免疫功能失调为ＶＭＣ发病及影响预后的重要因素。     

CVB3m对免疫功能低下小鼠心肌损伤的病理观察

目的 观察免疫功能低下小鼠感染ＣＶＢ３ｍ后心肌损伤的特点。方法 雄性昆明小鼠随机分成４组，１对照组；Ⅱ照射组；Ⅲ病毒组；Ⅳ照射＿病毒组。照射组４ＧｙＸ－射线全身照射；病毒组常规腹腔接种ＣＶＢ３ｍ；照射＋病毒组先照射，丁照射后次日接咎ＣＶＢ３ｍ。观察各组小鼠接种病毒后第３、７、１４、２１天心肌光镜改变，及第１４天心肌电镜改变。结果 病毒组小鼠注射病毒后第７在，病变最重，病毒性心肌炎（ＶＭＣ）检出率为     

老年肺心病急性发作期外周血T细胞亚群及B细胞的改变

老年肺心病急性发作期外周血Ｔ细胞亚群及Ｂ细胞的改变熊韬张焕景代水田武汉市第三医院内科（４３００６０）图１Ｔ细胞亚群改变与ＰａＯ２的关系图２Ｔ细胞亚群改变与ＰａＣＯ２的关系表Ｔ细胞亚群及Ｂ细胞变化（ｘ±ｓ％）ＣＤ３＋ＣＤ＋４ＣＤ＋８ＣＤ＋４／ＣＤ＋８Ｂ...     

小鼠病毒性心肌炎细胞凋亡相关蛋白的表达

目的 探讨嗜鼠心肌Ｃｏｘｓａｃｋｉｅ Ｂ３病毒（ＣＶＢ３ｍ）诱导的ＢＡＬＢ／Ｃ小鼠病毒性心肌炎（ＶＭＣ）细胞凋亡的机制。方法 给ＢＡＬＢ／Ｃ小鼠腹腔接种０．１ｍｌ１００ＴＣＤ５０ＣＶＢ３ｍ，感染后批断脊处死小鼠，应用双重荧光染色技术、原位末端标记法（ＴＵＮＥＬ）和共聚焦显微镜对小鼠心肌细胞凋亡及部分相关蛋白表达进行检测。结果 ＣＶＢ３ｍ可诱发小鼠ＶＭＣ。感染病毒后９～１２ｄ病变达到高峰，检出率达１     

Coxsackie B3病毒诱导人心肌细胞凋亡

体外培养胎儿心肌细胞，待细胞贴壁成层时接种Ｃｏｘｓａｃｋｉｅ Ｂ３病毒（ＣＶＢ３），２４小时后收集细胞。应用电镜、ＤＮＡ凝胶电泳分析及原位末端标记法（ＴＵＮＥＬ）检测发现，感染病毒心肌细胞每视野均有７０％以上呈阳性染色，ＤＮＡ凝胶电泳出现“梯状带”；对照细胞呈现阳性染色者每视野不超过５％，ＤＮＡ凝视电泳只见一条正常基因组ＤＮＡ带。实验结果提示，ＣＶＢ３可诱导体外培养人心肌细胞的凋亡。     

Dose-Dependent Destruction of A1 Cells by Anti-A1

Abstract. In a patient of subgroup A₂ the serum contained an unusually potent anti-A₁, giving the following reactions with A₁ red cells in vitro: agglutination of saline-suspended cells up to a temperature of 32°C; a positive indirect antiglobulin test (complement only) at 37°C and lysis of enzyme-treated cells at 37°C. A series of tests was carried out to estimate the ability of the antibody to destroy varying amounts of A₁ red cells in vivo . When about 0.55 ml of red cells was injected, about 65% of the cells were destroyed within 30 min; 2 days later when 18.9 ml of cells were injected, only about 45% were destroyed within 30 min; 5 days after this when a whole unit of A₁ red cells was transfused, survival at 24 h was about 90%. This last figure may indicate that destruction of red cells by anti-A₁ was negligible since at the time of the transfusion of the whole unit the patient was bleeding into her gastrointestinal tract. On the other hand, the titre of anti-A₁ appeared to be declining spontaneously during the period in which tests were carried out so that, if the whole unit of A₁ blood had been transfused at the beginning of this period, survival might have been less good. Nevertheless, from the observed difference in survival between the 0.55 ml and 18.9 ml doses it seems safe to conclude that, even if the unit had been transfused at the time when the antibody concentration was maximal, the percentage of cells destroyed would have been small.     

Activity of IgG and IgM ABO Antibodies against Some Weak A (A3, Ax, Aend) and Weak B (B3, Bx) Red Cells

The IgG and IgM anti-A and anti-B activities from several immune and non-immune O, A and B sera were tested against a panel of weak (A (A3, AX, AND Aend) and weak B (B3 and Bx) red cells. In all cases it is the IgM which agglutinated optimally Ax (or Bx) cells, while IgG and IgM anti-A (or anti-B) reacted similarly with A3 and Aend (or B3) cells. The agglutinating activity of all these ABO antibodies was found straightly related to their association constant for the A (or the B) receptor.     

Arteriolar Contribution to Microcirculatory CO2/O2 Exchange

The role of arterioles and capillaries in microcirculatory gas exchange was evaluated using a multicompartmental model for O₂-CO₂ transport in the rat skeletal microcirculation. Model predictions were examined to investigate the effects of model formulation and model parameter values. The factors in model formulation included radial blood diffusion resistance, the discrete nature of capillary blood, and the method of determining compartmental fluxes. A comparison with earlier models in the literature indicated that, by refining the method for determining compartmental fluxes, the CO₂ flux contribution ratio of arterioles versus capillaries (F_a/F_c) increased by 52% during rest and diminished by 34% during moderate exercise. It also resulted in negative venular fluxes during exercise. Incorporating radial blood diffusion resistance into the model lead to a decrease of up to 43% in F_a /F_c. It also resulted in a decrease in central arteriole-venule shunt. Including the discrete nature of capillary blood into the model caused a small increase in F_a /F_c. Results indicated similar effects of these factors on oxygen F_a/F_c. Model parameters whose effects were investigated included metabolic rate (M), blood flow rate (Q), ratio of arteriolar diffusion conductance versus capillary diffusion conductance (E_a/E_c), the magnitude of arteriolar diffusion conductances (E_a), and the CO₂/O₂ respiratory quotient (Qu). Simulation results suggested that Q was a major factor responsible for the variations in F_a /F_c when the rest/exercise state of rat skeletal muscle changes. E_a and Qu were also responsible for differences in model predictions for different body organs or animal species. Our model predicts capillary dominance in both CO₂ and O₂ exchange and reveals the existence, under certain conditions, of negative venular flux contribution.     

OKT4/OKT8 Ratio in Asymptomatic Heroin Addicts

The OKT₄ (helper) and OKT₈ (suppressor) lymphocytic subpopulations were enumerated in a sample of 60 asymptomatic drug addicts and in 17 controls. No significant differences in the ratio could be found that could not be explained by the action of HIV. It can be concluded that heroin itself was not responsible for any alteration in the T₄/T₈ ratio in the population considered.     

Impact of glucose concentration on cardiac ventricular repolarization under IKr/IKs blocking agents

Drug-induced QT interval prolongation is a condition likely to be aggravated by diabetes. The objective of this study was to evaluate how glucose concentration may modulate drug effects on ventricular repolarization and on cardiac repolarizing potassium currents. Guinea pig hearts were Langendorff-retroperfused and monophasic action potential duration (MAPD) was measured. Glucose (1, 5 or 20 mmol/L) was tested with either dofetilide (a specific I_Kr blocker), chromanol 293B (a specific I_Ks blocker) or both. Effects of glucose (1, 5 or 20 mmol/L) on I_Kr blockade mediated by dofetilide were also measured in HERG-transfected HEK293 cells in the absence vs presence of the P-glycoprotein drug transporter, using the whole cell patch-clamp technique. Our results suggest that both hypo- and hyperglycemia potentiate the MAPD-prolonging and I_Kr-blocking properties of dofetilide. P-glycoprotein drug extrusion efficacy appears as a key determinant of dofetilide's I_Kr-blocking effect. This efficacy appears to be affected by glucose concentration, particularly hyperglycemia.     

不损伤黏膜层的腹腔镜内镜联合剥除胃小间质瘤初步研究

目的 初步探讨在不损伤黏膜层的情况下，腹腔镜内镜联合胃小间质剥除术的可行性。 方法 2015年1月-2016年10月对15例胃小间质瘤患者在腹腔镜术中进行内镜检查，进行定位和黏膜下注射，然后在腹腔镜下行不损伤黏膜层的剥除术，回顾性总结分析这15例治疗效果。 结果 15例小间质瘤患者均顺利完成手术，无一例因术中并发症中转开腹。手术时间（53.4±15.8）min，术中出血（19.7±13.3）ml。所有患者均未放置胃管，术后流质饮食时间（1.6±0.5）d，术后住院时间（3.3±0.6）d；术后所有标本假包膜完整，病理均为胃间质瘤，其中12例极低危险度，2例低危险度，1例中危险度。 结论 腹腔镜内镜联合胃小间质瘤剥除术短期治疗效果满意，并且术后康复快，符合微创外科和快速康复理念，是胃小间质瘤治疗的一个新的选择。     

动脉样硬化患者CD11a、CD11b和CD18表达的初步研究

目的：探讨动脉粥样硬化和白细胞功能相关抗原（LFA－1即CD11a/CD18）以及巨噬细胞分化抗原（Mac-1即CD11b/CD18)等细胞粘附分子表达的关系。方法：用碱磷酶抗磷磷酶（A－PAAP）桥联酶免疫细胞化学染色法检测冠心病患者外周血单个核细胞表面的CD11a、CD11b和CD18。结果心病患者CD11a、CD11b、CD18阳性细胞数显著增高,血浆LDL水平与CD11a、CD18阳性单个核细胞数明显相关,正常人单个核细胞经各因素处理后除内毒素对CD11b外其它被测细胞粘附分子阳性细胞数可升至病人水平,且冠心病患者单个核细胞之细胞粘附分子也以相同的反应性再度增高。结论单个核细胞表面CD11a、CD11b和CD18特别是CD11a和CD18的表达增强可能和动脉粥样硬化的发生有关。