血清尿酸水平对大鼠阴茎勃起功能的影响

目的观察高尿酸血症对阴茎勃起功能的影响。方法将健康成年雄性SD大鼠40只随机均分为正常对照组和实验组2组。对照组给予普通饮食,实验组给予高尿酸饮食。4周后分别检测各组大鼠阴茎勃起功能,并检测血清尿酸和睾酮(T),以及阴茎海绵体组织NO含量。结果实验组血清尿酸水平(57.44±5.3)较对照组(39.85±3.6)显著升高,差异具有统计学意义(P0.05);而阴茎勃起功能、血清睾酮水平,以及阴茎海绵体组织NO含量均较对照组降低,差异具有统计学意义(P0.05)。结论高尿酸血症大鼠阴茎勃起功能显著降低,其机制可能与尿酸通过抑制血清睾酮及阴茎海绵体NO水平有关。     

OSAHS导致勃起功能障碍与血清NO和NOS相关性的临床观察

目的:临床探讨阻塞性睡眠呼吸暂停低通气综合征(OSAHS)导致阴茎勃起功能障碍(ED)与外周血一氧化氮(NO)和一氧化氮合酶(NOS)水平的相关性。方法:经多导睡眠监测仪(PSG)及阴茎夜间勃起功能测定(NPT),诊断OSAHS合并ED患者60名作为ED组,同时随机选择30名健康志愿者设立为健康组,分析ED组和健康组血清NO和NOS水平,及轻、中、重ED组血清NO、NOS水平、阴茎血容积变化率及IIEF-5总分差异,并将轻、中、重ED组阴茎血容积变化率及IIEF-5总分分别与各自血清NO和NOS水平进行相关性分析。结果:ED组的外周血NO和NOS水平均低于无ED组和健康组,差异具有统计学意义(P0.05)。阴茎血容积变化率与NO(r=0.356,P0.05)、NOS(r=0.398,P0.05)均为正相关;IIEF-5总分与NO(r=0.402,P0.05)、NOS(r=0.423,P0.05)均为正相关。结论:OSAHS导致的ED患者与患者外周血NO和NOS水平变化情况的具有相关性,且呈正相关,说明外周血NO和NOS水平的降低是OSAHS导致ED的内在病理基础。     

硫化氢信号在糖尿病大鼠阴茎海绵体中表达的研究

目的探讨胱硫醚-γ-裂解酶(CSE)、胱硫醚-β-合成酶(CBS)与硫化氢在糖尿病大鼠阴茎海绵体组织中的表达及与大鼠勃起功能的关系。方法从30只SD雄性大鼠中随机选取15只设为糖尿病组,15只设为对照组。糖尿病组大鼠饲喂高糖、高脂饮食4周,而后腹腔注射链脲佐菌素制备二型糖尿模型,造模成功后继续饲喂高糖、高脂饮食4周。对照组大鼠给予正常饮食4周后,腹腔内注射相同剂量生理盐水,继续正常饮食喂养4周。8周后,分别测定两组大鼠阴茎海绵体内压/平均动脉压(ICP/MAP),采用Elisa检测血浆和阴茎海绵体内源性H_2S含量,采用免疫组化和Western blot分析CSE和CBS在阴茎海绵体内的表达。结果与对照组大鼠相比,糖尿病组大鼠ICP/MAP比值显著降低(P0.05);血浆H_2S浓度,阴茎海绵体组织H_2S含量显著降低(P0.05);阴茎组织内CSE和CBS的含量也明显减低(P0.05)。结论糖尿病组大鼠高血糖可以通过抑制CBS和CSE的表达,降低阴茎海绵体组织内H_2S浓度,引起阴茎海绵体舒张功能障碍,导致阴茎勃起功能受损。     

负压吸引对阴茎海绵体动脉血流影响的研究

利用手握式负压吸引器对阴茎进行间断抽吸并彩色多普勒超声探测12例心理性勃起功能障碍患者会阴部阴茎海绵体动脉直径、血流速度、血流阻力指数等,根据公式:τm=η×4×Vm/Dr计算阴茎勃起各阶段的动脉血流剪切力.结果表明负压吸引前自然状态下双侧海绵体动脉血流剪切力(单位dynes/cm2)平均13.40±4.58;负压吸引下阴茎Ⅱ度勃起状态的剪切力平均为15.64±4.62;Ⅳ度勃起状态下剪切力平均为15.66±4.03.阴茎Ⅱ级勃起状态和Ⅳ级勃起状态平均剪切力较自然状态高,有统计学意义(P=0.032)、(P=0.047).Ⅳ级勃起状态平均剪切力略高于Ⅱ级勃起状态,但无统计学意义(P=0.985).揭示彩色多普勒超声结合负压吸引器可动态检测阴茎海绵体动脉血流剪切力,负压吸引可增加阴茎动脉血流量.     

多囊卵巢综合征不孕症患者血清氧化应激和IGF-I检测的临床意义

目的探讨血清氧化应激和IGF-I水平在多囊卵巢综合征不孕症患者中检测的临床价值。方法选取112例多囊卵巢综合征不孕症患者为病例组,选取因男方少弱精子症需要进行夫精宫腔内人工授精的妇女50例为对照组,对比分析两组研究对象的氧化应激及IGF-I等指标水平是否存在差异,以及相关指标与多囊卵巢综合征患者促排卵及妊娠结局等的相关性。结果病例组患者SOD水平低于对照组(68.64±12.22vs113.62±23.60μU/ml),血清MDA、 LPO、GSH-Px和IGF-1水平高对照组(13.25±4.67VS6.7 3±2.75 U/L、8.34±1.27 VS 3.48±0.68μU/ml、211.86±32.27 VS 161.48±21.68 U/L、262.83±29.94 VS 149.38±12.50μg/L),差异具有统计学意义(P0.01);病例组中排卵率以及妊娠率均明显低于对照组(78.57 VS 94.00%、15.18 VS36.00%),差异具有统计学意义(P0.05);而流产率及多胎率高于对照组(4.46 VS 2.00%、3.57VS 0.00%),但差异无统计学意义(P0.05));血清氧化应激MDA、 LPO、GSH-Px和IGF-1水平与多囊卵巢综合征不孕症患者排卵率、妊娠率呈负相关相关(r值分别为-0.731,-0.562,-0.689,-7.786;-0.685,-0.680,-0.776,-0.742),差异具有统计学意义(P0.05);与流产率以及早产率呈正相关(r值分别为0.687, 0.664, 0.685, 6.038; 0.587, 0.634, 0.564, 0.656),差异具有统计学意义(P0.05);血清SOD与多囊卵巢综合征不孕症患者排卵率、妊娠率呈正相关(r=7.834),差异具有统计学意义(P0.05);与流产率以及早产率呈正相关(r=-0.834),差异具有统计学意义(P0.05);临床妊娠组血清MDA,LPO,GSH-Px和IGF-1水平高于未妊娠组,而血清SOD水平明显高于未妊娠组,差异具有统计学意义(P0.01)。结论多囊卵巢综合征患者血清氧化应激和IGF-I与促排卵疗效和妊娠结局具有一定的相关性,上述指标可能在临床多囊卵巢综合征不孕症患者的治疗中具有一定的参考价值。     

枸杞多糖对糖尿病大鼠阴茎勃起功能障碍的防治作用

目的:观察分析枸杞多糖对糖尿病大鼠阴茎勃起功能障碍的治疗和预防效果。方法:采用枸杞多糖分别对大鼠建立糖尿病模型前后进行预防和治疗,并设立糖尿病组(DM组)和正常对照组,测定各组大鼠尾静脉血血糖变化,观察各组大鼠阴茎勃起情况,电击刺激后检测海绵体内压力(ICP)和平均动脉压(MAP),并检测大鼠阴茎组织NOS活力和NO含量和cG MP水平。结果:DM组、枸杞多糖治疗组、枸杞多糖防治组治疗前后血糖水平、勃起率与正常对照组相比较差异均显著(P0.05),枸杞多糖防治组建模时间、治疗前后血糖水平与枸杞多糖治疗组及DM组相比较差异均显著(P0.05),枸杞多糖防治组治疗后勃起率与DM组相比较差异均显著(P0.05),DM组、枸杞多糖治疗组、枸杞多糖防治组治疗后NOS、NO、cG MP水平ICP及ICP/MAP与正常对照组相比较差异均显著(P0.05),枸杞多糖防治组及枸杞多糖治疗组NOS、NO、cG MP水平、ICP及ICP/MAP与DM组相比较差异均显著(P0.05),枸杞多糖防治组大鼠ICP/MAP与枸杞多糖治疗组相比较差异显著(P0.05)。结论:枸杞多糖具有降低血糖和预防血糖升高的作用,并且对糖尿病大鼠阴茎勃起功能障碍具有明显的防治效果。     

糖尿病性勃起障碍大鼠阴茎海绵体平滑肌组织形态学及α-SMA表达量的变化

目的：通过大鼠实验总结糖尿病性勃起障碍阴茎海绵体平滑肌组织形态学和α-SMA表达变化。方法：将20只正常大鼠分为观察组（12只）和对照组（8只）。观察组为糖尿病性勃起障碍大鼠,对照组为正常大鼠。取两组阴茎海绵体组织,采用Masson三色法和免疫荧光化学染色法测定平滑肌/胶原纤维相对含量和α-SMA表达。结果：对照组阴茎勃起率为100%,观察组阴茎勃起率为8.33%。观察组在2.5、5.0、7.5V电刺激下Max ICP/MAP比值均显著低于对照组（P〈0.05）。观察组平滑肌/胶原纤维比值为0.16±0.04,α-SMA表达为0.25±0.17,均显著低于对照组,有统计学差异（P〈0.05）。结论：糖尿病性勃起障碍大鼠的平滑肌数量减少,胶原纤维明显增长,α-SMA表达受抑。上述情况可能是勃起障碍的主要原因之一。     

RigiScan阴茎硬度测量仪对54例高脂血症患者阴茎夜间勃起指标的检测观察

目的分析RigiScan阴茎硬度测量仪对高脂血症患者阴茎夜间勃起指标的检测结果。方法选取2019年4月1日至2020年3月31日浙江中医药大学附属温州中西医结合医院筛选的符合条件的54例高脂血症患者纳入高脂血症组,另选同期30例血脂正常男性纳入血脂正常组。均采用RigiScan阴茎硬度测量仪进行夜间阴茎勃起功能试验(NPT),测试2晚。并于测试前进行国际勃起功能指数-5(IIEF-5)问卷评估。观察两组测量后夜间阴茎勃起次数、阴茎勃起总持续时间,头部、根部硬度≥60%持续时间,头部、根部的周径变化及时间强度测量值(TAU),并进行比较。结果高脂血症组IIEF-5评分[(16.81±6.41)分]低于血脂正常组[(20.73±3.54)分],差异具有统计学意义(P0.05)。高脂血症组夜间阴茎勃起次数[(4.0±1.3)次]、阴茎勃起总持续时间[(98.21±24.86)min]与血脂正常组[(4.3±1.5)次,(103.55±20.85)min]比较,差异无统计学意义(P0.05)。高脂血症组夜间阴茎头部硬度≥60%持续时间[(46.7±14.4)min]、根部硬度≥60%持续时间[(43.1±22.6)min]均低于血脂正常组[(75.3±17.6)min,(79.2±20.1)min],差异具有统计学意义(P0.01)。高脂血症组阴茎头部的周径变化[(49.69±21.23)%]、根部的周径变化[(40.21±16.16)%]均低于血脂正常组[(73.33±15.57)%,(62.69±17.85)%],差异具有统计学意义(P0.05);头部TAU(43.37±15.17)、根部TAU(49.10±13.58)均低于血脂正常组(75.72±13.61,68.20±10.15),差异具有统计学意义(P0.01)。结论高脂血症能显著影响男性勃起功能,是导致男性器质性勃起功能障碍的重要致病因素之一。     

过敏性紫癜患儿血清中IGF-1,PTX3和GAL-3水平及临床意义

目的:探讨过敏性紫癜(HSP)患儿血清中胰岛素生长因子(IGF-1)、五正聚蛋白(PTX)3及半乳糖蛋白(GAL)-3的水平变化及其临床意义.方法:采用酶联免疫吸附法(ELISA)检测30例HSP患儿急性期(急性期组)和恢复期(恢复期组)及20例正常健康儿童(正常对照组)血清中IGF-1、PTX3和GAL-3水平.结果:①正常对照组、HSP急性期组、恢复期组患儿血清IGF-1,PTX3和GAL-3水平分别为(68.73±28.87)μg/L、(176.17±149.91)μg/L与(89.56±33.27)μg/L、(10.55±3.79)μg/L、(18.14±15.10)μg/L与(10.71±4.82)μg/L、(19.31±5.79)μg/L、(32.80±25.30)μg/L与(18.15±6.76)μg/L.急性期组与正常对照组比较,差异均有统计学意义(P < 0.01,P < 0.05,P < 0.05).急性期组与恢复期组比较差异亦,有统计学意义(P < 0.001).恢复期组与正常对照组比较,仅IGF-1差异亦有统计学意义(P < 0.01),而PTX3和GAL-3差异无统计学意义(P > 0.1).②急性期肾损组与急性期无肾损组比较时,IGF-1,PTX3和GAL-3分别为(273.56±188.32)μg/L、(101.69±66.38)μg/L,P < 0.01;(19.62±18.99)μg/L、(16.94±13.16)μg/L,P > 0.1;(40.46±34.07)μg/L、(34.91±24.03)μg/L,P > 0.1,IGF-1.HSP患儿血清IGF-1与24 h尿蛋白水平成正相关(r = 0.85,P < 0.01),血清PTX3与血清C反应蛋白(CRP)水平无关联(r = 0.09,P < 0.1).结论:IGF-1、PTX3和GAL-3可作为HSP疾病早期活动性指标,对判定其病情、预后和指导治疗均有一定意义.     

不同中医治法对功能性勃起功能障碍模型大鼠eNOS mRNA、Cx43 mRNA表达的影响

目的:基于中医"肝藏血、主疏泄"藏象理论,研究不同治法对功能性勃起功能障碍模型大鼠阴茎海绵体eNOS mRNA、Cx43 mRNA表达的影响。方法:选取经交配后证实有正常勃起功能的雄性SD大鼠50只,随机分为空白组、模型组、补血活血法组、疏肝理气法组、疏肝理气活血法组,共五组,每组10只,采用尾部放血和悬吊激惹方法配合低铁饮食制备肝郁血虚勃起功能障碍模型,造模的第1d开始,在悬吊结束后1h,养血法组、疏肝法组与疏肝养血法组分别给予灌胃四物汤煎剂3.75g/kg、逍遥散煎剂9.22g/kg、四物汤与逍遥散合方煎剂12.96g/kg,空白组、模型组给予灌胃等体积生理盐水,1次/d,连续14d,末次灌胃24h后处死大鼠,取阴茎海绵体组织,HE染色观察阴茎海绵体组织病理学,PCR观察阴茎海绵体eNOS mRNA、Cx43 mRNA。结果:各组阴茎海绵体组织病理学改变均不明显;与空白组相比,模型组阴茎平滑肌组织Cx43mRNA、eNOS mRNA表达明显较低(P0.05);与模型组相比,疏肝理气活血法组能显著提高阴茎平滑肌组织中Cx43 mRNA、eNOS mRNA表达(P0.05),疏肝理气法组可显著提高阴茎平滑肌组织中Cx43 mRNA的表达(P0.05),补血活血法组阴茎平滑肌组织中Cx43 mRNA、eNOS mRNA表达无统计学意义(P0.05)。结论:疏肝理气活血和疏肝理气治法方药能够提高Cx43、eNOS活性,而补血活血治法方药不能提高Cx43、eNOS活性。     

HIF-1,GLUY-1在恶性肿瘤组织中表达的研究进展

缺氧诱导因子-1(HIF-1)是缺氧条件下广泛存在于哺乳动物和人体内的一种核转录因子,能与缺氧反应基因上特定位点结合,启动靶基因的转录,使机体对缺氧产生一系列适应性反应。葡萄糖转运蛋白-1(GLUT-1)是葡萄糖转运蛋白家族成员之一,主要介导细胞内外的跨膜转运,调节葡萄糖摄取,对糖代谢的调节等功能起到关键的作用。二者与一些恶性肿瘤的发生、发展、甚至转移及预后都有密切关系。     

The morphologic spectrum of germline‐mutated BAP1‐inactivated melanocytic tumors includes lesions with conventional nevic melanocytes: A case report and review of literature

Germline mutations in BRCA1‐associated protein 1 (BAP1) are associated with several neoplasms, including BAP1‐inactivated melanocytic tumors (BIMTs). BIMTs are classically described as biphenotypic melanocytic proliferations with BAP1‐deficient large epithelioid and rhabdoid melanocytes showing various degrees of cytologic atypia. This morphology has been traditionally classified as “spitzoid” despite the various differences between these lesions and the more classic Spitz nevi. Herein, we report a case of an otherwise healthy 11‐year‐old female patient with a family history of several malignancies who presented with multiple pink to brown papules. Histologic and immunohistochemical evaluation identified three lesions with loss of nuclear BAP1 staining. The histologic spectrum of these lesions included junctional spitzoid cells within a triphenotypic proliferation and a separate lesion composed entirely of dermal small to medium‐sized epithelioid melanocytes with maturation. BAP1 gene sequencing revealed a germline frameshift pathogenic BAP1 mutation, denoted c.1717delC. This case provides further evidence that not all BIMTs conform to classic morphological criteria and that the morphologic spectrum includes lesions resembling conventional nevi. As BIMTs can serve as an early marker of the BAP1 hereditary tumor predisposition syndrome, we believe a need exists for a more comprehensive combined clinical and pathological approach for BIMT identification.     

Assessment of gene expression levels of proopiomelanocortin (POMC) and melanocortin‐1 receptor (MC1R) in vitiligo

Proopiomelanocortin (POMC) and melanocortin 1 receptor (MC1R) are regulators of melanogenesis and pigmentation. Our objective was to estimate their levels, searching for a possible role of the melanocortin system in vitiligo. This study included 40 vitiligo patients and 40 controls. Skin biopsies were taken from lesional and non‐lesional skin of patients and from the non‐sun exposed skin of controls to detect the expression of POMC and MC1R using quantitative real‐time polymerase chain reaction. Both factors were significantly lower in lesional than non‐lesional skin and controls, while they were significantly higher in non‐lesional skin than in controls. There was a statistically significant positive correlation between lesional levels of POMC and MC1R, as well as between non‐lesional levels of POMC and MC1R in the patients. On the other hand, we found a statistically significant negative correlation between the lesional and non‐lesional levels of POMC, as well as between the lesional and non‐lesional levels of MC1R in the patients. As a conclusion, the melanocortin system could play a role in the pathogenesis of vitiligo or could be affected as the end result of the disease.     

他克莫司对UVA照射后HaCaT细胞表达ICAM-1的影响

目的研究他克莫司(TM)对长波紫外线(UVA)照射后HaCaT细胞表达细胞间黏附分子-1(ICAM-1)的影响,以探讨TM治疗光敏性皮肤病的作用机制。方法采用ELASI和免疫组化法检测在不同强度UVA照射后,HaCaT细胞表达ICAM-1的情况。结果与未经UVA照射组细胞比较,未加TM组经UVA照射24h后,HaCaT细胞表达ICAM-1水平明显升高(P<0.01),而加入TM组的ICAM-1表达明显受到抑制(P<0.01)。结论TM对UVA照射后HaCaT细胞表达ICAM-1有抑制作用,提示对UVA引起的炎症有抑制作用。     

Intercellular Adhesion Molecule-1 on Cultured Human Melanoma Cells: Influence of Cytokines

Adhesion molecules such as intercellular adhesion molecule-1 (ICAM-1) and its counter-receptor, lymphocyte function associated antigen-1 (LFA-1), play very important roles in immune responses. In this study, the effects of cytokines on cultured human melanoma cells (MMG2) were examined, especially focussing on the expression of ICAM-1 on MMG2 and lymphocyte adhesion to MMG2. Both the expression of ICAM-1 and HLA-DR on MMG2 increased after treatment with IFN-gamma. ICAM-1 expression began to increase earlier than HLA-DR expression. TNF-alpha and IL-1 beta also increased the expression of ICAM-1 on MMG2. However, these cytokines did not increase the expression of HLA-DR. IFN-gamma had a dose dependent effect on lymphocyte adhesion to MMG2. Pretreatment of IFN-gamma treated MMG2 with 84H10 (anti-ICAM-1 antibody) or pretreatment of lymphocytes with either SPV-L7 (anti-LFA-1 alpha antibody) or IOT10 (anti-LFA-1 beta antibody) inhibited the lymphocyte adhesion to MMG2. These results suggest that ICAM-1 molecules induced on melanoma cells by IFN-gamma can interact with LFA-1 molecules on lymphocytes.     

9‐cis retinoic acid is the ALDH1A1 product that stimulates melanogenesis

Aldehyde dehydrogenase 1A1 (ALDH1A1), an enzyme that catalyses the conversion of lipid aldehydes to lipid carboxylic acids, plays pleiotropic roles in UV‐radiation resistance, melanogenesis and stem cell maintenance. In this study, a combination of RNAi and pharmacologic approaches were used to determine which ALDH1A1 substrates and products regulate melanogenesis. Initial studies revealed that neither the UV‐induced lipid aldehyde 4‐hydroxy‐2‐nonenal nor the ALDH1A1 product all‐trans retinoic acid appreciably induced melanogenesis. In contrast, both the ALDH1A1 substrate 9‐cis retinal and its corresponding product 9‐cis retinoic acid potently induced the accumulation of MITF mRNA, Tyrosinase mRNA and melanin. ALDH1A1 depletion inhibited the ability of 9‐cis retinal but not 9‐cis retinoic acid to stimulate melanogenesis, indicating that ALDH1A1 regulates melanogenesis by catalysing the conversion of 9‐cis retinal to 9‐cis retinoic acid. The addition of potent ALDH1A inhibitors (cyanamide or Angeli's salt) suppressed Tyrosinase and MITF mRNA accumulation in vitro and also melanin accumulation in skin equivalents, suggesting that 9‐cis retinoids regulate melanogenesis in the intact epidermis. Taken together, these studies not only identify cyanamide as a potential novel treatment for hyperpigmentary disorders, but also identify 9‐cis retinoic acid as a pigment stimulatory agent that may have clinical utility in the treatment of hypopigmentary disorders, such as vitiligo.     

子宫肌瘤患者体内MMP-9、TGF-β1和EGF水平变化特点及意义

目的探讨子宫肌瘤患者体内基质金属蛋白酶-9(MMP-9)、转化生长因子-β1(TGF-β1)和表皮生长因子(EGF)水平及意义。方法选取2017年1月至2018年10月湖南省妇幼保健院诊治的80例子宫肌瘤患者作为研究对象。将这80例患者设为观察组,同时选取80例子宫无异常女性作为对照组,检测两组血清MMP-9,TGF-β1和EGF水平。结果观察组患者血清MMP-9、TGF-β1和EGF明显高于对照组患者,差异具有统计学意义(P<0.05);黏膜下子宫肌瘤患者血清MMP-9、TGF-β1和EGF明显高于肌壁间子宫肌瘤患者,差异具有统计学意义(P<0.05);不同直径、分期及数量子宫肌瘤患者血清MMP-9、TGF-β1和EGF比较差异无统计学意义(P>0.05);子宫肌瘤患者MMP-9、TGF-β1和EGF呈正相关,差异具有统计学意义(r=0.5682和0.527,P<0.05),MMP-9和TGF-β1呈正相关,差异具有统计学意义(r=0.322,P<0.05)。结论子宫肌瘤患者体内MMP-9,TGF-β1和EGF水平明显升高,3者之间存在相关性,同时与子宫肌瘤位置有一定关系。     

血清抗Dsg1特异性抗体水平变化与落叶型天疱疮病情的关系

目的探讨落叶型天疱疮(PF)患者外周血血清IIF滴度、Dsg1-ELISA指数与病情变化的相关性。方法监测8例落叶型天疱疮患者不同时期病情变化及其相应时间点血清中特异抗体的间接免疫荧光(IIF)滴度和Dsg1-ELISA指数,并对结果进行分析。结果 7例(87.5%)患者病情评分与Dsg1-ELISA指数有显著性关联(P0.05),且与病情波动平行变化,4例(50%)患者病情评分与IIF滴度有显著性关联,与病情波动平行变化。结论 Dsg1-ELISA指数可作为PF病情的监测和调整治疗方案的重要参考。     

寻常性银屑病皮损表皮Dsg1 mRNA的表达

银屑病特征性的病理改变不仅与角质形成细胞(KC)异常增殖有关,也与KC凋亡异常有关[1-2].凋亡是由天冬氨酸特异性半胱氨酸蛋白酶家族(caspase)成员介导的蛋白酶级联反应过程,其中caspase-3是主要的凋亡效应分子.桥粒芯糖蛋白1(Dsg1)是caspase底物蛋白.我们采用RT-PCR方法从mRNA水平检测Dsg1与caspase-3在银屑病皮损中的表达,以探讨Dsg1在银屑病皮损表皮KC凋亡中的作用.