新型钛合金阳极氧化后对成骨细胞增殖、分化的影响

目的研究新型医用钛合金Ti-24Nb-4Zr-7.9Sn(TNZS)经过阳极氧化(AD)技术处理后对成骨细胞增殖和分化的影响。方法将人成骨样MG63细胞接种于Ti6Al4V、TNZS、AD-TNZS表面,采用MTT法检测成骨细胞的增殖情况,考马斯亮蓝G250染色法检测细胞总蛋白质含量,对硝基苯磷酸盐法检测碱性磷酸酶(ALP)活性。结果人成骨样MG63细胞在Ti6Al4V、TNZS以及AD-TNZS表面均能良好生长,细胞增殖率、细胞总蛋白含量未见明显差异(P>0.05);但培养至4、7d时,AD-TNZS组细胞ALP活性明显高于其他组(P<0.05)。结论阳极氧化处理的TNZS钛合金可促进成骨细胞的分化。     

阳极氧化处理后的新型钛合金体外诱导实验及对成骨细胞早期附着的影响

目的：研究新型医用钛合金Ti-24Nb-4Zr-7．9Sn（TNZS）经过阳极氧化（AD）技术处理后体外诱导活性及对人成骨样MG63细胞早期附着的影响。方法：采用阳极氧化处理TNZS合金，对其进行模拟体液（SBF）浸泡实验，分析其表面元素成分及相结构的变化；并将人成骨样MG63细胞接种于Ti6Al4V、TNZS、AD—TNZS表面，观察细胞的早期附着及形态学变化。结果：AD—TNZS在SBF中浸泡6d后，材料表面有羟基磷灰石形成。在MG63成骨样细胞接种60、120min时，人成骨样MG63细胞在AD—TNZS表面的附着率均明显高于Ti6Al4V和TNZS表面附着率（P〈0．05），并表现出良好的形态。结论：阳极氧化后的新型医用钛合金在模拟体液中可诱导羟基磷灰石形成；对成骨细胞的早期附着有一定的促进作用。     

中间普氏菌培养上清对牙龈成纤维细胞RANKL／OPG表达的影响

目的：探讨中间普氏菌培养上清对牙龈成纤维细胞核因子-κB受体活化因子配体（receptor activator for nuclear factor-κB ligand,RANKL）、骨保护素（osteoprotegerin,OPG）表达的影响。方法：将系列浓度的中间普氏菌培养上清作用于牙龈成纤维细胞,显微镜下观察细胞的变化。实时定量 RT-PCR法和 Western blot法分别检测 RANKL、OPG mRNA及蛋白水平的表达,并计算 RANKL/OPG的比值。结果：牙龈成纤维细胞的数量随中间普氏菌培养上清浓度的增加而减少,50μg/mL 时,显微镜下没有活细胞存在。在mRNA水平上和蛋白水平上,RANKL/OPG的比值在12．5μg/mL处理组均高于对照组,差异具有统计学意义（P＜0．01）。结论：中间普氏菌培养上清可诱导人牙龈成纤维细胞表达 RANKL 和 OPG,破坏 RANKL/OPG比例的平衡,影响破骨细胞分化的细胞因子微环境,在破骨细胞分化和牙周炎骨吸收中起着一定的调节作用。     

TNF-α和PGE2联合在人牙周膜成纤维细胞RANKL/OPG mRNA表达中的作用

目的:探讨不同浓度组合的肿瘤坏死因子-α(TNF-α)和前列腺素E2(PGE2)在人牙周膜成纤维细胞(HPDLFs)的核因子-κB受体活化因子配体(RANKL)及其饵受体骨保护素(OPG)mRNA表达中的作用。方法:将体外培养的人牙周膜成纤维细胞用不同浓度组合的TNF-α和PGE2处理24h,通过荧光定量逆转录聚合酶链反应(FQ-RT-PCR)来观察人牙周膜成纤维细胞RANKL、OPG mRNA表达水平。结果:不同浓度组合对人牙周膜成纤维细胞RANKL/OPG mRNA表达有促进作用,尤以10ng/mL TNF-α 10-7mol/L PGE2组合对RANKL mRNA表达的促进作用及RANKL/OPG比值的增强作用最为明显。结论:10ng/mL TNF-α和10-7mol/L PGE2联合具有协同作用,通过促进人牙周膜成纤维细胞RNAKL/OPG表达量,可能在调节牙周炎引起的牙槽骨吸收中起到重要的作用。     

MT01/PEN复合物对人成骨样细胞MG63表达骨保护蛋白和核因子κB受体活化因子配体的影响

目的 应用聚乙烯亚胺阳离子聚合物（PEN）载体装载寡脱氧核苷酸MT01,制备MT01/PEN复合物,检测该复合物对人成骨样细胞MG63表达骨保护蛋白（OPG）和核因子κB受体活化因子配体（RANKL）的影响。方法 制备3种不同配比的MT01/PEN（质量比分别为1∶2、1∶4、1∶6）复合物,以全硫代化修饰MT01（MT01-s）和未修饰的MT01作阳性对照,分别转染MG63细胞。采用酶联免疫吸附测定法和real-time聚合酶链反应法分别检测培养24、48、72 h时各组上清液及细胞内OPG和RANKL的表达水平。结果 经MT01/PEN复合物转染后,上清液及细胞内OPG表达水平均升高（P<0.05）;多数组中RANKL表达水平降低,而OPG/RANKL比值呈升高趋势（P<0.05）;不同质量配比的MT01/PEN均对MG63细胞的成骨具有影响,其中质量比为1∶6时作用最明显。结论 应用PEN作为基因载体装载MT01可增强MT01对MG63细胞的促成骨作用。     

牙槽裂两侧正畸牙移动后RANKL和OPG表达的研究

目的：研究牙移动后牙槽裂隙两侧破骨细胞核因子κB受体活化因子配体（RANKL）和骨保护素（OPG）的表达和作用.方法：选用4只4月龄雄性Beagle犬建立牙槽裂模型,正畸牵引裂隙两侧牙齿向裂隙区移动,实时荧光定量聚合酶链反应（real-time PCR）检测裂隙两侧牙槽骨中RANKL和OPG mRNA的表达水平.结果：正畸牵引16～20周后,牙槽裂间隙缩小以至关闭,原裂隙处有新骨生成.裂隙两侧牙槽骨中RANKL和OPG mRNA表达增加.结论：牙槽裂两侧牙齿受正畸牵引后,RANKL与OPG参与骨重建,裂隙处骨改建活跃.此方法为修复牙槽裂提供新的思路.     

可降解型种植体支抗材料对人成骨样细胞OPG/RANKL mRNA表达的影响

目的:评价两种可降解型种植体支抗材料聚乳酸酯和羟基磷灰石对人成骨样细胞MG-63的细胞毒性,检测OPG/RANKL mRNA的表达量。方法:选择人成骨样细胞MG-63作为细胞毒性的实验对象。MTT法检测细胞活力,评价两种可降解型种植体支抗材料聚乳酸酯和羟基磷灰石微粒对人成骨样细胞增殖的影响。用realtime-PCR检测人成骨样细胞内OPG/RANKL mRNA的表达量。结果:钛粒子对细胞生长具有一定的抑制作用,羟基磷灰石和聚乳酸酯颗粒对细胞的增殖没有明显的副作用。钛粒子可以有效的诱导成骨样细胞OPG的表达,同时也增强了RANKL的表达,并随着粒子作用时间的增加,作用效果也相应增加。与钛粒子相比较,羟基磷灰石和聚乳酸酯颗粒对细胞OPG/RANKL mRNA表达无明显的影响,且随着作用时间的增加及在不同浓度的干预下,细胞OPG/RANKL mRNA表达也无明显的影响。结论:聚乳酸酯和羟基磷灰石对破骨细胞增殖和OPG/RANKL mRNA的表达无明显影响。     

降钙素基因相关肽对兔成骨细胞OPG/RANKL mRNA表达的影响

目的：探讨外源性降钙素基因相关肽(CGRP)对成骨细胞核因子-κB受体活化因子配体(RANKL)及其饵受体骨保护素(OPG)基因表达的影响，了解CGRP在破骨细胞性骨吸收调节中的作用机制。方法：将体外培养的兔成骨细胞用不同浓度的CGRP处理24h，通过半定量逆转录聚合酶链反应(RT-PCR)来观察成骨细胞两种目的基因(OPG，RANKL)的mRNA表达强度变化。结果：CGRP呈剂量依赖性的刺激成骨细胞OPG mRNA表达，同时亦下调RANKL mRNA表达。结论：CGRP通过调节成骨细胞OPG／RANKL的比值可间接地调节破骨细胞活性，从而影响骨代谢。     

骨保护素的生物学特性及其在口腔领域的研究进展

骨保护素(osteoprotegerin,OPG)是1997年Simonet等发现的一种能阻止破骨细胞分化,促进骨形成的关键因子。骨保护素及其配体核因子-κB受体活化因子配体(receptor activator of nuclear factorκB ligand,RANKL),阐明了破骨     

miR-223调控人牙周膜成纤维细胞中RANKL表达的功能初探

目的：探讨miR?223对人牙周膜成纤维细胞中核因子?κB受体活化因子配体（ RANKL）的调控作用。方法：构建过表达miR?223的人牙周膜成纤维细胞,Western blot及实时定量RT?PCR检测人牙周膜成纤维细胞内RANKL和骨保护素（ OPG）的表达,计算RANKL／OPG比值的改变。结果：过表达miR?223的人牙周膜成纤维细胞,RANKL的表达明显下调,RANKL／OPG比值下降。结论：miR?223可调控RANKL的表达,破坏RANKL／OPG比例的平衡,改变破骨细胞的微环境,影响破骨细胞分化。     

Oral cancer: molecular technologies for risk assessment and diagnosis

Purpose: The effective biomarkers related to diagnosis, metastasis, drug resistance and irradiation sensitivity of oral cancers will help the pathologist and oncologist to determine the molecular taxonomy diagnosis and design the individualization treatment for the patients with oral cancers.     

Stimulated whole saliva components in children with Down syndrome

The authors report on the components of stimulated whole saliva from children with Down syndrome—including pH, flow rate, sialic acid and protein concentrations, and amylase and peroxidase activity. Saliva samples were collected from 35 children aged 6–10 years. Of the participants, 17 had Down syndrome and 18 did not. To stimulate saliva production, the children chewed a piece of parafilm for 10 minutes before the sample was collected. Soon after collecting the saliva sample, the authors measured pH using a portable pH-meter. Sialic acid levels were determined with a thiobarbituric acid assay. Protein content was determined with Folin's phenol reagent. Amylase was assayed and the authors measured the maltose produced by the breakdown of starch and peroxidase using ortho-dianisidine.
No statistically significant difference was observed in levels of sialic acid (free and total) between the two groups. Protein concentration was about 36% higher in the group with Down syndrome. However, the salivary flow rate, pH, and amylase and peroxidase activities were lower among the children with Down syndrome.     

柠檬精油对牙周致病菌抑制作用及其安全性初步研究

目的:探讨柠檬精油对牙周致病菌的体外抗菌活性及对细胞增殖的影响。方法:采用微量液体稀释法测定柠檬精油对Pg、Fn、Aa、Pi的最小抑菌浓度(minimal inhibitory concentration,MIC)及最小杀菌浓度(minimum bactericidal concentration,MBC);以较低浓度的MIC为标准稀释LEO作为实验组,采用MTT法测定柠檬精油对HUVECs的毒性作用,明确抑菌浓度下LEO的安全性。结果:柠檬精油对牙周主要致病菌均有抑菌作用,Pg、Fn、Aa、Pi的MIC分别是9.0 g/L、4.5 g/L、4.5 g/L、9.0 g/L,Aa、Fn的 MBC是9.0 g/L,Pg、Pi的MBC未测得。1/2MIC、1/20MIC浓度的LEO能够抑制人脐静脉内皮细胞的生长,而低于1/200MIC浓度的LEO则对人脐静脉内皮细胞的生长没有影响,其中1/200MIC浓度的LEO作用明显优于0.02%的CHX。结论:体外环境中,柠檬精油对牙周致病菌Pg、Fn、Aa、Pi具有抗菌活性,低浓度应用对机体相对安全。     

Computer-assisted planning for maxillofacial prosthodontic rehabilitation

Computerized tomography (CT) planning and the use of CT derived surgical templates for implant placement have shown promise for restoring function within months after surgical reconstruction of acquired post-oncologic defects.     

牙髓再生的研究现状

目前,根管治疗术在临床上已得到了广泛的应用并成功的保存了患牙,但其仍有许多术后并发症。随着组织工程技术在口腔领域的应用,构建一种组织工程化牙髓进行牙髓-牙本质复合体的再生治疗成为可能。近年来国内外已有很多关于牙髓再生的研究,并且已经取得了一定成果。本文将对目前国内外牙髓再生的研究进展现状作一综述。     

Effect of Pingyangmycin on human venous malformation endothelial cells

Purpose: Venous malformations are common vascular anomalies with a propensity of the head and neck. Intralesional injection of Pingyangmycin (PYM, bleomycin A5 hydrochloride) is a widely used sclerotherapy method for the treatment of venous malformation.     

Clinical and pathological aspects on some odontogenic tumors

Odontogenic tumors constitute a very diverse group of lesions that reflects the complex processes of odontogenesis. Controversies over their classification/subtyping, terminology and diagnosis have been persisted, which has direct bearings on therapeutic and/or prognostic implications.     

Can you be too old for oral implants? An update on ageing and plasticity in the oro‐facial sensorimotor system

This review focuses on the capacity of the brain for plasticity and the utility and efficacy of oral implants in helping to restore oro‐facial sensorimotor functions, especially in elderly patients. The review first outlines the components of the oro‐facial sensorimotor system which encompasses both oro‐facial tissues and a number of brain regions. One such region is the sensorimotor cortex that controls the activity of the numerous oro‐facial skeletal muscles. These muscles are involved in a number of functions including reflexes and the more complex sensorimotor functions of mastication, swallowing and speech. The review outlines the use by the brain of sensory inputs from oro‐facial receptors in order to provide for exquisite sensorimotor control of the activity of the oro‐facial muscles. It highlights the role in this sensorimotor control played by periodontal mechanoreceptors and their sensory inputs to the brain, and how oral implants in concert with the plastic capacity of the brain may, at least in part, compensate for reduced sensorimotor functioning when teeth are lost. It outlines findings of ageing‐related decrements in oro‐facial sensorimotor functions and control. The changes in oro‐facial tissues and the brain that underlie these ageing‐related functional alterations are also considered, along with adaptive and compensatory processes that utilise the brain's capacity for plasticity. The review also notes the evidence t hat rehabilitation that incorporates adjunctive approaches such as sensorimotor training paradigms in addition to oral prostheses such as implants may enhance these processes and help maintain or facilitate recovery of sensorimotor functioning in the elderly.     

Tumor microenvironment in oral cancer: novel approaches for immunotherapy

Squamous cell carcinoma of the head and neck (SCCHN), which arises from the squamous mucosal epithelium of the oral cavity, pharynx and larynx, is a major health problem in the US and other parts of the world, especially in developing countries.